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Protein

Putative callose synthase 8

Gene

CALS8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals (By similarity).By similarity

Catalytic activityi

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G14570-MONOMER.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.
TCDBi9.B.119.1.3. the glycan synthase, fks1 (fks1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative callose synthase 8 (EC:2.4.1.34)
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 4
Gene namesi
Name:CALS8
Synonyms:GSL4
Ordered Locus Names:At3g14570
ORF Names:MIE1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14570.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 530CytoplasmicSequence analysisAdd BLAST530
Transmembranei531 – 551HelicalSequence analysisAdd BLAST21
Topological domaini552 – 565ExtracellularSequence analysisAdd BLAST14
Transmembranei566 – 586HelicalSequence analysisAdd BLAST21
Topological domaini587 – 602CytoplasmicSequence analysisAdd BLAST16
Transmembranei603 – 623HelicalSequence analysisAdd BLAST21
Topological domaini624 – 648ExtracellularSequence analysisAdd BLAST25
Transmembranei649 – 669HelicalSequence analysisAdd BLAST21
Topological domaini670 – 707CytoplasmicSequence analysisAdd BLAST38
Transmembranei708 – 728HelicalSequence analysisAdd BLAST21
Topological domaini729 – 759ExtracellularSequence analysisAdd BLAST31
Transmembranei760 – 780HelicalSequence analysisAdd BLAST21
Topological domaini781 – 1544CytoplasmicSequence analysisAdd BLAST764
Transmembranei1545 – 1565HelicalSequence analysisAdd BLAST21
Topological domaini1566 – 1595ExtracellularSequence analysisAdd BLAST30
Transmembranei1596 – 1616HelicalSequence analysisAdd BLAST21
Topological domaini1617 – 1620CytoplasmicSequence analysis4
Transmembranei1621 – 1641HelicalSequence analysisAdd BLAST21
Topological domaini1642 – 1688ExtracellularSequence analysisAdd BLAST47
Transmembranei1689 – 1709HelicalSequence analysisAdd BLAST21
Topological domaini1710 – 1715CytoplasmicSequence analysis6
Transmembranei1716 – 1736HelicalSequence analysisAdd BLAST21
Topological domaini1737 – 1790ExtracellularSequence analysisAdd BLAST54
Transmembranei1791 – 1811HelicalSequence analysisAdd BLAST21
Topological domaini1812 – 1819CytoplasmicSequence analysis8
Transmembranei1820 – 1840HelicalSequence analysisAdd BLAST21
Topological domaini1841 – 1856ExtracellularSequence analysisAdd BLAST16
Transmembranei1857 – 1877HelicalSequence analysisAdd BLAST21
Topological domaini1878 – 1884CytoplasmicSequence analysis7
Transmembranei1885 – 1905HelicalSequence analysisAdd BLAST21
Topological domaini1906 – 1928ExtracellularSequence analysisAdd BLAST23
Transmembranei1929 – 1949HelicalSequence analysisAdd BLAST21
Topological domaini1950 – 1976CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003345801 – 1976Putative callose synthase 8Add BLAST1976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi1676N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LUD7.
PRIDEiQ9LUD7.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LUD7. baseline and differential.
GenevisibleiQ9LUD7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G14570.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LUD7.
SMRiQ9LUD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1095 – 1098Poly-Leu4

Sequence similaritiesi

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ9LUD7.
KOiK11000.
OMAiIIMACHD.
OrthoDBiEOG0936004B.
PhylomeDBiQ9LUD7.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 2 hits.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LUD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHEIVPVDP IDVPSTSYSR PILGPREDSP ERATEFTRSL TFREHVSSEP
60 70 80 90 100
FDSERLPATL ASEIQRFLRI ANLVESEEPR IAYLCRFHAF EIAHHMDRNS
110 120 130 140 150
TGRGVRQFKT SLLQRLELDE EFTVRRRKEK SDVRELKRVY HAYKEYIIRH
160 170 180 190 200
GAAFNLDNSQ REKLINARRI ASVLYEVLKT VTSGAGPQAI ADRESIRAKS
210 220 230 240 250
EFYVPYNILP LDKGGVHQAI MHLPEIKAAV AIVRNTRGLP PPEEFQRHQP
260 270 280 290 300
FLDLFEFLQY AFGFQNGNVA NQREHLILLL SNTIIRQPQK QSSAPKSGDE
310 320 330 340 350
AVDALMKKFF KNYTNWCKFL GRKNNIRLPY VKQEALQYKT LYIGLYLLIW
360 370 380 390 400
GEASNLRFMP ECLCYIFHHM AYELHGVLTG AVSMITGEKV APAYGGGHES
410 420 430 440 450
FLADVVTPIY MVVQKEAEKN KNGTADHSMW RNYDDLNEFF WSLECFEIGW
460 470 480 490 500
PMRPEHDFFC VESSETSKPG RWRGMLRFRK QTKKTDEEIE DDEELGVLSE
510 520 530 540 550
EQPKPTSRWL GKTNFVETRS FWQIFRSFDR MWSFFVLSLQ ALIIMACHDV
560 570 580 590 600
GSPLQVFNAN IFEDVMSIFI TSAILKLIKG ILDIIFKWKA RNTMPINEKK
610 620 630 640 650
KRLVKLGFAA MWTIILPVLY SHSRRKYICY FTNYKTWLGE WCFSPYMVAV
660 670 680 690 700
TIYLTGSAIE LVLFFVPAIS KYIETSNHGI FKTLSWWGQP RLYVGRGMQE
710 720 730 740 750
TQVSQFKYTF FWILVLLTKF AFSYAFEIKP LIEPTRLIMK VGVRNYEWHE
760 770 780 790 800
IFPEVKSNAA AIVAVWAPIM VVYFMDTQIW YSVYCTIFGG LYGVLHHLGE
810 820 830 840 850
IRTLGMLRGR FHTLPSAFNA SLIPHSTKDE KRRKQRGFFP FNLGRGSDGQ
860 870 880 890 900
KNSMAKFVLV WNQVINSFRT EDLISNKELD LMTMPLSSEV LSGIIRWPIF
910 920 930 940 950
LLANKFSTAL SIAKDFVGKD EVLYRRIRKD EYMYYAVKEC YESLKYILQI
960 970 980 990 1000
LVVGDLEKKI ISGIINEIEE SIRQSSLLEE FKMAELPALH DKCIELVQLL
1010 1020 1030 1040 1050
VEGSAEQLQV EKSEELHGKL VKALQDIFEL VTNDMMVHGD RILDLLQSRE
1060 1070 1080 1090 1100
GSGEDTGIFM RVIEPQLFES YGEWRCIHFP LPDSASLSEQ IQRFLLLLTV
1110 1120 1130 1140 1150
KDSAMDIPEN LDARRRLSFF ATSLFMDMPD APKVRNMMSF SVLTPHYQED
1160 1170 1180 1190 1200
INYSTNELHS TKSSVSIIFY MQKIFPDEWK NFLERMGCDN LDALKKEGKE
1210 1220 1230 1240 1250
EELRNWASFR GQTLSRTVRG MMYCREALKL QAFLDMADDE DILEGYKDVE
1260 1270 1280 1290 1300
RSNRPLAAQL DALADMKFTY VVSCQMFGAQ KSSGDPHAQD ILDLMIKYPS
1310 1320 1330 1340 1350
LRVAYVEERE EIVLDVPKKV YYSILVKAVN GFDQEIYRVK LPGPPNIGEG
1360 1370 1380 1390 1400
KPENQNHAIV FTRGEALQTI DMNQDHYLEE AFKMRNLLQE FLRNRGRRPP
1410 1420 1430 1440 1450
TILGLREHIF TGSVSSLAWF MSYQETSFVT IGQRLLANPL RVRFHYGHPD
1460 1470 1480 1490 1500
VFDRIFHITR GGISKSSRTI NLSEDVFAGY NTTLRRGCIT YNEYLQVGKG
1510 1520 1530 1540 1550
RDVGLNQISK FEAKVANGNS EQTISRDIYR LGQRFDFFRM LSCYFTTIGF
1560 1570 1580 1590 1600
YFSSLISVIG IYIYLYGQLY LVLSGLQKTL ILEAKVKNIK SLETALASQS
1610 1620 1630 1640 1650
FIQLGLLTGL PMVMEIGLEK GFLIAFQDFI LMQLQLAAFF FTFSLGTKTH
1660 1670 1680 1690 1700
YFGRTILHGG AKYRPTGRKV VVFHANFSEN YRLYSRSHFI KGFELMILLV
1710 1720 1730 1740 1750
VYELFKHTSQ SNMAYSFITF SVWFMSFTWL CAPFLFNPSG FTWEIIVGDW
1760 1770 1780 1790 1800
RDWNRWIKEQ GGIGIQQDKS WQSWWNDEQA HLRGSGVGAR CLEIILSLRF
1810 1820 1830 1840 1850
FVYQYGLVYH LDITQSNTNI IVYALSWVVI LATFFTVKAV DLGRQLFSTR
1860 1870 1880 1890 1900
KHLVFRFFKV FVFVSILTII ITLANICHLS VKDLLVSCLA FLPTGWGLIL
1910 1920 1930 1940 1950
IAQAVRPKIE GTSLWEFTQV LARAYDYGMG VVLFAPMAIL AWLPIISAFQ
1960 1970
TRFLFNEAFN RRLQIQPILA GKKKNR
Length:1,976
Mass (Da):228,484
Last modified:May 20, 2008 - v2
Checksum:i2DEDE6CE91425957
GO

Sequence cautioni

The sequence BAB02389 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023038 Genomic DNA. Translation: BAB02389.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75539.1.
RefSeqiNP_188075.2. NM_112317.3. [Q9LUD7-1]

Genome annotation databases

EnsemblPlantsiAT3G14570.1; AT3G14570.1; AT3G14570. [Q9LUD7-1]
GeneIDi820683.
GrameneiAT3G14570.1; AT3G14570.1; AT3G14570.
KEGGiath:AT3G14570.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023038 Genomic DNA. Translation: BAB02389.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75539.1.
RefSeqiNP_188075.2. NM_112317.3. [Q9LUD7-1]

3D structure databases

ProteinModelPortaliQ9LUD7.
SMRiQ9LUD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G14570.1.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.
TCDBi9.B.119.1.3. the glycan synthase, fks1 (fks1) family.

Proteomic databases

PaxDbiQ9LUD7.
PRIDEiQ9LUD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14570.1; AT3G14570.1; AT3G14570. [Q9LUD7-1]
GeneIDi820683.
GrameneiAT3G14570.1; AT3G14570.1; AT3G14570.
KEGGiath:AT3G14570.

Organism-specific databases

TAIRiAT3G14570.

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ9LUD7.
KOiK11000.
OMAiIIMACHD.
OrthoDBiEOG0936004B.
PhylomeDBiQ9LUD7.

Enzyme and pathway databases

BioCyciARA:AT3G14570-MONOMER.

Miscellaneous databases

PROiQ9LUD7.

Gene expression databases

ExpressionAtlasiQ9LUD7. baseline and differential.
GenevisibleiQ9LUD7. AT.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 2 hits.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALS8_ARATH
AccessioniPrimary (citable) accession number: Q9LUD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: November 30, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.