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Q9LUD7 (CALS8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative callose synthase 8
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 4
Gene names
Name:CALS8
Synonyms:GSL4
Ordered Locus Names:At3g14570
ORF Names:MIE1.7
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1976 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence BAB02389.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LUD7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19761976Putative callose synthase 8
PRO_0000334580

Regions

Topological domain1 – 530530Cytoplasmic Potential
Transmembrane531 – 55121Helical; Potential
Topological domain552 – 56514Extracellular Potential
Transmembrane566 – 58621Helical; Potential
Topological domain587 – 60216Cytoplasmic Potential
Transmembrane603 – 62321Helical; Potential
Topological domain624 – 64825Extracellular Potential
Transmembrane649 – 66921Helical; Potential
Topological domain670 – 70738Cytoplasmic Potential
Transmembrane708 – 72821Helical; Potential
Topological domain729 – 75931Extracellular Potential
Transmembrane760 – 78021Helical; Potential
Topological domain781 – 1544764Cytoplasmic Potential
Transmembrane1545 – 156521Helical; Potential
Topological domain1566 – 159530Extracellular Potential
Transmembrane1596 – 161621Helical; Potential
Topological domain1617 – 16204Cytoplasmic Potential
Transmembrane1621 – 164121Helical; Potential
Topological domain1642 – 168847Extracellular Potential
Transmembrane1689 – 170921Helical; Potential
Topological domain1710 – 17156Cytoplasmic Potential
Transmembrane1716 – 173621Helical; Potential
Topological domain1737 – 179054Extracellular Potential
Transmembrane1791 – 181121Helical; Potential
Topological domain1812 – 18198Cytoplasmic Potential
Transmembrane1820 – 184021Helical; Potential
Topological domain1841 – 185616Extracellular Potential
Transmembrane1857 – 187721Helical; Potential
Topological domain1878 – 18847Cytoplasmic Potential
Transmembrane1885 – 190521Helical; Potential
Topological domain1906 – 192823Extracellular Potential
Transmembrane1929 – 194921Helical; Potential
Topological domain1950 – 197627Cytoplasmic Potential
Compositional bias1095 – 10984Poly-Leu

Amino acid modifications

Glycosylation16761N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 2DEDE6CE91425957

FASTA1,976228,484
        10         20         30         40         50         60 
MSHEIVPVDP IDVPSTSYSR PILGPREDSP ERATEFTRSL TFREHVSSEP FDSERLPATL 

        70         80         90        100        110        120 
ASEIQRFLRI ANLVESEEPR IAYLCRFHAF EIAHHMDRNS TGRGVRQFKT SLLQRLELDE 

       130        140        150        160        170        180 
EFTVRRRKEK SDVRELKRVY HAYKEYIIRH GAAFNLDNSQ REKLINARRI ASVLYEVLKT 

       190        200        210        220        230        240 
VTSGAGPQAI ADRESIRAKS EFYVPYNILP LDKGGVHQAI MHLPEIKAAV AIVRNTRGLP 

       250        260        270        280        290        300 
PPEEFQRHQP FLDLFEFLQY AFGFQNGNVA NQREHLILLL SNTIIRQPQK QSSAPKSGDE 

       310        320        330        340        350        360 
AVDALMKKFF KNYTNWCKFL GRKNNIRLPY VKQEALQYKT LYIGLYLLIW GEASNLRFMP 

       370        380        390        400        410        420 
ECLCYIFHHM AYELHGVLTG AVSMITGEKV APAYGGGHES FLADVVTPIY MVVQKEAEKN 

       430        440        450        460        470        480 
KNGTADHSMW RNYDDLNEFF WSLECFEIGW PMRPEHDFFC VESSETSKPG RWRGMLRFRK 

       490        500        510        520        530        540 
QTKKTDEEIE DDEELGVLSE EQPKPTSRWL GKTNFVETRS FWQIFRSFDR MWSFFVLSLQ 

       550        560        570        580        590        600 
ALIIMACHDV GSPLQVFNAN IFEDVMSIFI TSAILKLIKG ILDIIFKWKA RNTMPINEKK 

       610        620        630        640        650        660 
KRLVKLGFAA MWTIILPVLY SHSRRKYICY FTNYKTWLGE WCFSPYMVAV TIYLTGSAIE 

       670        680        690        700        710        720 
LVLFFVPAIS KYIETSNHGI FKTLSWWGQP RLYVGRGMQE TQVSQFKYTF FWILVLLTKF 

       730        740        750        760        770        780 
AFSYAFEIKP LIEPTRLIMK VGVRNYEWHE IFPEVKSNAA AIVAVWAPIM VVYFMDTQIW 

       790        800        810        820        830        840 
YSVYCTIFGG LYGVLHHLGE IRTLGMLRGR FHTLPSAFNA SLIPHSTKDE KRRKQRGFFP 

       850        860        870        880        890        900 
FNLGRGSDGQ KNSMAKFVLV WNQVINSFRT EDLISNKELD LMTMPLSSEV LSGIIRWPIF 

       910        920        930        940        950        960 
LLANKFSTAL SIAKDFVGKD EVLYRRIRKD EYMYYAVKEC YESLKYILQI LVVGDLEKKI 

       970        980        990       1000       1010       1020 
ISGIINEIEE SIRQSSLLEE FKMAELPALH DKCIELVQLL VEGSAEQLQV EKSEELHGKL 

      1030       1040       1050       1060       1070       1080 
VKALQDIFEL VTNDMMVHGD RILDLLQSRE GSGEDTGIFM RVIEPQLFES YGEWRCIHFP 

      1090       1100       1110       1120       1130       1140 
LPDSASLSEQ IQRFLLLLTV KDSAMDIPEN LDARRRLSFF ATSLFMDMPD APKVRNMMSF 

      1150       1160       1170       1180       1190       1200 
SVLTPHYQED INYSTNELHS TKSSVSIIFY MQKIFPDEWK NFLERMGCDN LDALKKEGKE 

      1210       1220       1230       1240       1250       1260 
EELRNWASFR GQTLSRTVRG MMYCREALKL QAFLDMADDE DILEGYKDVE RSNRPLAAQL 

      1270       1280       1290       1300       1310       1320 
DALADMKFTY VVSCQMFGAQ KSSGDPHAQD ILDLMIKYPS LRVAYVEERE EIVLDVPKKV 

      1330       1340       1350       1360       1370       1380 
YYSILVKAVN GFDQEIYRVK LPGPPNIGEG KPENQNHAIV FTRGEALQTI DMNQDHYLEE 

      1390       1400       1410       1420       1430       1440 
AFKMRNLLQE FLRNRGRRPP TILGLREHIF TGSVSSLAWF MSYQETSFVT IGQRLLANPL 

      1450       1460       1470       1480       1490       1500 
RVRFHYGHPD VFDRIFHITR GGISKSSRTI NLSEDVFAGY NTTLRRGCIT YNEYLQVGKG 

      1510       1520       1530       1540       1550       1560 
RDVGLNQISK FEAKVANGNS EQTISRDIYR LGQRFDFFRM LSCYFTTIGF YFSSLISVIG 

      1570       1580       1590       1600       1610       1620 
IYIYLYGQLY LVLSGLQKTL ILEAKVKNIK SLETALASQS FIQLGLLTGL PMVMEIGLEK 

      1630       1640       1650       1660       1670       1680 
GFLIAFQDFI LMQLQLAAFF FTFSLGTKTH YFGRTILHGG AKYRPTGRKV VVFHANFSEN 

      1690       1700       1710       1720       1730       1740 
YRLYSRSHFI KGFELMILLV VYELFKHTSQ SNMAYSFITF SVWFMSFTWL CAPFLFNPSG 

      1750       1760       1770       1780       1790       1800 
FTWEIIVGDW RDWNRWIKEQ GGIGIQQDKS WQSWWNDEQA HLRGSGVGAR CLEIILSLRF 

      1810       1820       1830       1840       1850       1860 
FVYQYGLVYH LDITQSNTNI IVYALSWVVI LATFFTVKAV DLGRQLFSTR KHLVFRFFKV 

      1870       1880       1890       1900       1910       1920 
FVFVSILTII ITLANICHLS VKDLLVSCLA FLPTGWGLIL IAQAVRPKIE GTSLWEFTQV 

      1930       1940       1950       1960       1970 
LARAYDYGMG VVLFAPMAIL AWLPIISAFQ TRFLFNEAFN RRLQIQPILA GKKKNR

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[4]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB023038 Genomic DNA. Translation: BAB02389.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75539.1.
IPIIPI00517860.
RefSeqNP_188075.2. NM_112317.2.

3D structure databases

ProteinModelPortalQ9LUD7.
ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9LUD7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G14570.1; AT3G14570.1; AT3G14570.
GeneID820683.
GenomeReviewsGene locus AT3G14570 in contig BA000014_GR.
KEGGath:AT3G14570.

Organism-specific databases

TAIRAt3g14570.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
InParanoidQ9LUD7.
PhylomeDBQ9LUD7.

Gene expression databases

GenevestigatorQ9LUD7.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1_N.
[Graphical view]
Gene3DG3DSA:1.25.40.270. G3DSA:1.25.40.270. 1 hit.
KOK11000.
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS8_ARATH
AccessionPrimary (citable) accession number: Q9LUD7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents