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Protein

Protein OBERON 2

Gene

OBE2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with OBE1, required for the maintenance and/or establishment of both the shoot and root meristems, probably by controlling the expression of the meristem genes such as WUS, PLT1 and PLT2 and of genes required for auxin responses. Promotes cell meristematic activity via the WUSCHEL-CLAVATA pathway. Involved in the development of the basal pole and in auxin-mediated root and vascular development in the embryo. Confers sensitivity to turnip mosaic virus (TuMV) probably by promoting viral movement and multiplication via interaction with TuMV VPg.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri226 – 29166PHD-typeAdd
BLAST

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • maintenance of root meristem identity Source: TAIR
  • maintenance of shoot apical meristem identity Source: TAIR
  • primary root development Source: TAIR
  • regulation of gene expression Source: TAIR
  • root meristem specification Source: TAIR
  • transport of virus in host, cell to cell Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein OBERON 2
Alternative name(s):
Potyvirus VPg-interacting protein 1
Gene namesi
Name:OBE2
Synonyms:PVIP1
Ordered Locus Names:At5g48160
ORF Names:MIF21.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48160.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. When associated with OBE1 disruption, plants exhibit premature termination of the shoot meristem and impaired root apical meristem establishment, leading to a diminutive phenotype characterized by an absence of roots and defective development of the vasculature.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Protein OBERON 2PRO_0000399747Add
BLAST

Proteomic databases

PaxDbiQ9LUB7.
PRIDEiQ9LUB7.

PTM databases

iPTMnetiQ9LUB7.

Expressioni

Tissue specificityi

Expressed in roots, seedlings, stems, leaves, flowers and siliques, especially in the vasculature.2 Publications

Developmental stagei

First observed in the embryo proper at the four-cell stage. Later expressed throughout the embryo from the eight-cell to the bent-cotyledon stages. Until the torpedo stage of development, mostly concentrated at the root pole. Hardly detected in the suspensor. Present in seedling roots. In mature and fully differentiated young roots, restricted to root tips.2 Publications

Inductioni

By auxin in the root elongation zone.1 Publication

Interactioni

Subunit structurei

Self-interacts. Interacts with OBE1, OBE3 and OBE4. Binds to VPg of pea seed borne mosaic virus (PSbMV), turnip mosaic virus (TuMV) and lettuce mosaic virus (LMV), but not with VPg of tobacco etch virus (TEV), cowpea mosaic virus (CPMV), tomato black ring virus (TBRV) and grapevine fan leaf virus (GFLV).2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi20115. 5 interactions.
STRINGi3702.AT5G48160.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LUB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili416 – 524109Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 PHD-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri226 – 29166PHD-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGMY. Eukaryota.
ENOG4110PA9. LUCA.
HOGENOMiHOG000029805.
InParanoidiQ9LUB7.
OMAiEEKMRLY.
PhylomeDBiQ9LUB7.

Family and domain databases

InterProiIPR004082. OBERON.
IPR032535. Oberon_cc.
IPR032881. Oberon_PHD.
IPR001965. Znf_PHD.
[Graphical view]
PfamiPF16312. Oberon_cc. 1 hit.
PF07227. PHD_Oberon. 1 hit.
[Graphical view]
PIRSFiPIRSF025218. DUF1423_pln. 1 hit.
PRINTSiPR01544. ARATH130DUF.
SMARTiSM00249. PHD. 1 hit.
[Graphical view]
PROSITEiPS01359. ZF_PHD_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTSSGSNHP HQMLPPRQQQ RSGGGLETAL SLVSSDQEPR RESPAESASS
60 70 80 90 100
QETWPLGDTV AGKKSMSQKT EPDSMEQTVN VMHHVSNADK VSVRDIARER
110 120 130 140 150
VELVAERMHR LPDEFLDELK NGLKSILEGN VAQSVDEFMF LQKVVQSRTD
160 170 180 190 200
LSSVTLVRAH RVQLEILVAI NTGIQAFLHP NISLSQPSLI EIFVYKRCRN
210 220 230 240 250
IACQNQLPAD DCYCDICTNR KGFCNLCMCT ICNKFDFSVN TCRWIGCDLC
260 270 280 290 300
SHWTHTDCAI RDGQITTGSS AKNNTSGPGE IVFKCRACNR TSELLGWVKD
310 320 330 340 350
VFQHCAPNWD RESLMKELDF VSRIFRGSED QRGRKLFWKC EELIDKIKGG
360 370 380 390 400
LAEATAAKLI LMFFQEIESD SAKSFENGEG GRLMAPQDAC NRIAEVVQET
410 420 430 440 450
LRKMEIVAEE KMRMFKKARM ALETCDRELE DKAKEVSELK AERQKKKLQI
460 470 480 490 500
DELERIVRLK QAEADMFQLK ANEAKREADR LQRIVLAKMD KSEEEYASNY
510 520 530 540 550
LKQRLSEAEA EKQYLFEKIK LQENSRVASQ SSGGGGDPSQ VMMYSKIRDL
560 570
LQGYNLSPKV DPQLNERNPF RSNP
Length:574
Mass (Da):65,084
Last modified:October 1, 2000 - v1
Checksum:iA12D0462A0439442
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361D → G in BAE99299 (Ref. 4) Curated
Sequence conflicti361 – 3611L → F in BAH20018 (PubMed:19423640).Curated
Sequence conflicti493 – 4931E → G in BAE99299 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023039 Genomic DNA. Translation: BAA96996.1.
CP002688 Genomic DNA. Translation: AED95626.1.
CP002688 Genomic DNA. Translation: AED95627.1.
AK317346 mRNA. Translation: BAH20018.1.
AK317672 mRNA. Translation: BAH20332.1.
AK220595 mRNA. Translation: BAD94916.1.
AK227275 mRNA. Translation: BAE99299.1.
RefSeqiNP_001032031.1. NM_001036954.1.
NP_199627.1. NM_124190.3.
UniGeneiAt.28240.

Genome annotation databases

EnsemblPlantsiAT5G48160.1; AT5G48160.1; AT5G48160.
AT5G48160.2; AT5G48160.2; AT5G48160.
GeneIDi834868.
GrameneiAT5G48160.1; AT5G48160.1; AT5G48160.
AT5G48160.2; AT5G48160.2; AT5G48160.
KEGGiath:AT5G48160.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023039 Genomic DNA. Translation: BAA96996.1.
CP002688 Genomic DNA. Translation: AED95626.1.
CP002688 Genomic DNA. Translation: AED95627.1.
AK317346 mRNA. Translation: BAH20018.1.
AK317672 mRNA. Translation: BAH20332.1.
AK220595 mRNA. Translation: BAD94916.1.
AK227275 mRNA. Translation: BAE99299.1.
RefSeqiNP_001032031.1. NM_001036954.1.
NP_199627.1. NM_124190.3.
UniGeneiAt.28240.

3D structure databases

ProteinModelPortaliQ9LUB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20115. 5 interactions.
STRINGi3702.AT5G48160.1.

PTM databases

iPTMnetiQ9LUB7.

Proteomic databases

PaxDbiQ9LUB7.
PRIDEiQ9LUB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48160.1; AT5G48160.1; AT5G48160.
AT5G48160.2; AT5G48160.2; AT5G48160.
GeneIDi834868.
GrameneiAT5G48160.1; AT5G48160.1; AT5G48160.
AT5G48160.2; AT5G48160.2; AT5G48160.
KEGGiath:AT5G48160.

Organism-specific databases

TAIRiAT5G48160.

Phylogenomic databases

eggNOGiENOG410IGMY. Eukaryota.
ENOG4110PA9. LUCA.
HOGENOMiHOG000029805.
InParanoidiQ9LUB7.
OMAiEEKMRLY.
PhylomeDBiQ9LUB7.

Miscellaneous databases

PROiQ9LUB7.

Family and domain databases

InterProiIPR004082. OBERON.
IPR032535. Oberon_cc.
IPR032881. Oberon_PHD.
IPR001965. Znf_PHD.
[Graphical view]
PfamiPF16312. Oberon_cc. 1 hit.
PF07227. PHD_Oberon. 1 hit.
[Graphical view]
PIRSFiPIRSF025218. DUF1423_pln. 1 hit.
PRINTSiPR01544. ARATH130DUF.
SMARTiSM00249. PHD. 1 hit.
[Graphical view]
PROSITEiPS01359. ZF_PHD_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "A cysteine-rich plant protein potentiates Potyvirus movement through an interaction with the virus genome-linked protein VPg."
    Dunoyer P., Thomas C., Harrison S., Revers F., Maule A.
    J. Virol. 78:2301-2309(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POTYVIRUS VPG PROTEIN.
  6. "The Arabidopsis OBERON1 and OBERON2 genes encode plant homeodomain finger proteins and are required for apical meristem maintenance."
    Saiga S., Furumizu C., Yokoyama R., Kurata T., Sato S., Kato T., Tabata S., Suzuki M., Komeda Y.
    Development 135:1751-1759(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DIMERIZATION.
  7. "Arabidopsis plant homeodomain finger proteins operate downstream of auxin accumulation in specifying the vasculature and primary root meristem."
    Thomas C.L., Schmidt D., Bayer E.M., Dreos R., Maule A.J.
    Plant J. 59:426-436(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY AUXIN, SUBUNIT, INTERACTION WITH OBE1; OBE3 AND OBE4.

Entry informationi

Entry nameiOBE2_ARATH
AccessioniPrimary (citable) accession number: Q9LUB7
Secondary accession number(s): B9DH01, Q0WU99, Q570W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.