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Protein

Mitotic spindle checkpoint protein MAD2

Gene

MAD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: TAIR
  • regulation of mitotic cell cycle Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-ATH-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-ATH-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-ATH-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-179409. APC-Cdc20 mediated degradation of Nek2A.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint protein MAD21 Publication
Alternative name(s):
Mitotic arrest deficient protein 2
Gene namesi
Name:MAD21 Publication
Ordered Locus Names:At3g25980Imported
ORF Names:MPE11.15Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G25980.

Subcellular locationi

GO - Cellular componenti

  • chromocenter Source: TAIR
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
  • kinetochore Source: TAIR
  • kinetochore microtubule Source: UniProtKB
  • nuclear envelope Source: TAIR
  • nucleus Source: TAIR
  • spindle microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal growth conditions and stunted roots when grown in vitro in absence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Mitotic spindle checkpoint protein MAD2PRO_0000126121Add
BLAST

Proteomic databases

PaxDbiQ9LU93.
PRIDEiQ9LU93.

PTM databases

iPTMnetiQ9LU93.

Expressioni

Tissue specificityi

Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems.1 Publication

Inductioni

Cell cycle regulated expression with a distinct expression peak at the G2/M boundary.1 Publication

Gene expression databases

ExpressionAtlasiQ9LU93. baseline and differential.
GenevisibleiQ9LU93. AT.

Interactioni

Subunit structurei

Part of the mitotic checkpoint complex (MCC); interacts with MAD1, CDC20-1, CDC20-2 and CDC20-5. Interacts with BUBR1 at chromocenters and with BUB3.1. Interacts with EIF4B3 (PubMed:20706207).3 Publications

Protein-protein interaction databases

BioGridi7526. 34 interactions.
IntActiQ9LU93. 26 interactions.
STRINGi3702.AT3G25980.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LU93.
SMRiQ9LU93. Positions 12-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 198184HORMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAD2 family.Curated
Contains 1 HORMA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3285. Eukaryota.
ENOG410XSD7. LUCA.
HOGENOMiHOG000199586.
InParanoidiQ9LU93.
OMAiCLETNEV.
PhylomeDBiQ9LU93.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LU93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFVKVKKY
60 70 80 90 100
GLPMLLIEDE SVKSFMSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER
110 120 130 140 150
WNFRIETDNE VVDKGVSREK SDKEIMREIQ AIMRQVASSV TYLPCLDETC
160 170 180 190 200
VFDVLAYTDT DVAVPFTWIE SDPKLIANPQ MVKLHGFDTK IHKVDTLVSY

KNDEWDEEE
Length:209
Mass (Da):23,731
Last modified:October 1, 2000 - v1
Checksum:i91473FD5334C7A99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA. Translation: BAB01061.1.
CP002686 Genomic DNA. Translation: AEE77097.1.
BT010608 mRNA. Translation: AAQ89630.1.
AK175296 mRNA. Translation: BAD43059.1.
AK176363 mRNA. Translation: BAD44126.1.
RefSeqiNP_189227.1. NM_113502.3. [Q9LU93-1]
UniGeneiAt.37293.

Genome annotation databases

EnsemblPlantsiAT3G25980.1; AT3G25980.1; AT3G25980. [Q9LU93-1]
GeneIDi822195.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Arabidopsis APC/C subunits

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA. Translation: BAB01061.1.
CP002686 Genomic DNA. Translation: AEE77097.1.
BT010608 mRNA. Translation: AAQ89630.1.
AK175296 mRNA. Translation: BAD43059.1.
AK176363 mRNA. Translation: BAD44126.1.
RefSeqiNP_189227.1. NM_113502.3. [Q9LU93-1]
UniGeneiAt.37293.

3D structure databases

ProteinModelPortaliQ9LU93.
SMRiQ9LU93. Positions 12-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7526. 34 interactions.
IntActiQ9LU93. 26 interactions.
STRINGi3702.AT3G25980.1.

PTM databases

iPTMnetiQ9LU93.

Proteomic databases

PaxDbiQ9LU93.
PRIDEiQ9LU93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G25980.1; AT3G25980.1; AT3G25980. [Q9LU93-1]
GeneIDi822195.

Organism-specific databases

TAIRiAT3G25980.

Phylogenomic databases

eggNOGiKOG3285. Eukaryota.
ENOG410XSD7. LUCA.
HOGENOMiHOG000199586.
InParanoidiQ9LU93.
OMAiCLETNEV.
PhylomeDBiQ9LU93.

Enzyme and pathway databases

ReactomeiR-ATH-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-ATH-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-ATH-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-179409. APC-Cdc20 mediated degradation of Nek2A.

Miscellaneous databases

PROiQ9LU93.

Gene expression databases

ExpressionAtlasiQ9LU93. baseline and differential.
GenevisibleiQ9LU93. AT.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
IPR027097. Mad2.
[Graphical view]
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M., Shinozaki K., Ecker J.R.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division."
    Caillaud M.-C., Paganelli L., Lecomte P., Deslandes L., Quentin M., Pecrix Y., Le Bris M., Marfaing N., Abad P., Favery B.
    PLoS ONE 4:E6757-E6757(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BUBR1 AND BUB3.1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  6. Cited for: INTERACTION WITH EIF4B3.
  7. "Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana."
    Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K., Mergaert P., Kondorosi E.
    PLoS ONE 6:E20618-E20618(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC20-1; CDC20-2 AND CDC20-5.
  8. "Functional interaction between the Arabidopsis orthologs of spindle assembly checkpoint proteins MAD1 and MAD2 and the nucleoporin NUA."
    Ding D., Muthuswamy S., Meier I.
    Plant Mol. Biol. 79:203-216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD1, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMAD2_ARATH
AccessioniPrimary (citable) accession number: Q9LU93
Secondary accession number(s): Q67YV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.