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Protein

Mitotic spindle checkpoint protein MAD2

Gene

MAD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: TAIR
  • regulation of mitotic cell cycle Source: TAIR

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-ATH-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-ATH-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle checkpoint protein MAD21 Publication
Alternative name(s):
Mitotic arrest deficient protein 2
Gene namesi
Name:MAD21 Publication
Ordered Locus Names:At3g25980Imported
ORF Names:MPE11.15Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G25980.
TAIRilocus:2092125. AT3G25980.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal growth conditions and stunted roots when grown in vitro in absence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001261211 – 209Mitotic spindle checkpoint protein MAD2Add BLAST209

Proteomic databases

PaxDbiQ9LU93.

Expressioni

Tissue specificityi

Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems.1 Publication

Inductioni

Cell cycle regulated expression with a distinct expression peak at the G2/M boundary.1 Publication

Gene expression databases

ExpressionAtlasiQ9LU93. baseline and differential.
GenevisibleiQ9LU93. AT.

Interactioni

Subunit structurei

Part of the mitotic checkpoint complex (MCC); interacts with MAD1, CDC20-1, CDC20-2 and CDC20-5. Interacts with BUBR1 at chromocenters and with BUB3.1. Interacts with EIF4B3 (PubMed:20706207).3 Publications

Protein-protein interaction databases

BioGridi7526. 34 interactors.
IntActiQ9LU93. 26 interactors.
STRINGi3702.AT3G25980.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LU93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 198HORMAAdd BLAST184

Sequence similaritiesi

Belongs to the MAD2 family.Curated

Phylogenomic databases

eggNOGiKOG3285. Eukaryota.
ENOG410XSD7. LUCA.
HOGENOMiHOG000199586.
InParanoidiQ9LU93.
KOiK02537.
OMAiSEVPVEW.
OrthoDBiEOG09360MC5.
PhylomeDBiQ9LU93.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiView protein in InterPro
IPR003511. HORMA_dom.
IPR036570. HORMA_dom_sf.
IPR027097. Mad2.
PANTHERiPTHR11842:SF11. PTHR11842:SF11. 1 hit.
PfamiView protein in Pfam
PF02301. HORMA. 1 hit.
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiView protein in PROSITE
PS50815. HORMA. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LU93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFVKVKKY
60 70 80 90 100
GLPMLLIEDE SVKSFMSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER
110 120 130 140 150
WNFRIETDNE VVDKGVSREK SDKEIMREIQ AIMRQVASSV TYLPCLDETC
160 170 180 190 200
VFDVLAYTDT DVAVPFTWIE SDPKLIANPQ MVKLHGFDTK IHKVDTLVSY

KNDEWDEEE
Length:209
Mass (Da):23,731
Last modified:October 1, 2000 - v1
Checksum:i91473FD5334C7A99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023041 Genomic DNA. Translation: BAB01061.1.
CP002686 Genomic DNA. Translation: AEE77097.1.
BT010608 mRNA. Translation: AAQ89630.1.
AK175296 mRNA. Translation: BAD43059.1.
AK176363 mRNA. Translation: BAD44126.1.
RefSeqiNP_189227.1. NM_113502.3. [Q9LU93-1]
UniGeneiAt.37293.

Genome annotation databases

EnsemblPlantsiAT3G25980.1; AT3G25980.1; AT3G25980. [Q9LU93-1]
GeneIDi822195.
GrameneiAT3G25980.1; AT3G25980.1; AT3G25980.
KEGGiath:AT3G25980.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMAD2_ARATH
AccessioniPrimary (citable) accession number: Q9LU93
Secondary accession number(s): Q67YV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families