Q9LU91 (SKI14_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein SKIP14

Alternative name(s):
SKP1-interacting partner 14

Gene names

Name:	SKIP14
Ordered Locus Names:	At3g26000
ORF Names:	MPE11.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with CUL1, SKP1A/ASK1 and SPK1B/ASK2. Ref.5
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	F-box protein SKIP14		PRO_0000375237
Regions
	Domain	34 – 104	71	F-box; degenerate
	Compositional bias	368 – 444	77	Cys-rich
Experimental info
	Sequence conflict	402	1	K → E in AAM98151. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LU91 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CCD32A1DBA9039C4	FASTA	453	50,543
	10 20 30 40 50 60 MALNFSHRPF SSHLSEEPMM IANGNWCSSF DNGRKNTGGD ASSVDILDVL PSDPFGMDIN 70 80 90 100 110 120 NTFTAITGWL EDLEDDYNNQ YGRRRRDDIW IGDGNRQQLF AGLSFFWNNA MQFQSSGYSY 130 140 150 160 170 180 GSESLFGGAF DGSLFSTCKF PESSGENNGF GGALDGDGSC HGAFISASSV DEVLSHENAR 190 200 210 220 230 240 NGEVVGSSDR CNNGEEDAYV HPAIGFCLYH LRGKDLLSVS MVCKSLHTTV CDDTLLWKHI 250 260 270 280 290 300 HICRPLNEKI TEEALLHLTE RAQGTMQCLR IVDCCRITDD CLKRVVARNR QVVKIGVPGC 310 320 330 340 350 360 TRITIDGILS VLRDLKSAGK LQVKHLQLRG LFGVTKDHYD ELIDLLNIDN KVKQTIQKPR 370 380 390 400 410 420 FYHRGEACVS CDDDRALDIE MCPKCQNFKL VYDCPAEDCK GKKKGSEECR ACSLCIQRCY 430 440 450 HCGRCIIDTE YEEMFCLELL CAVCSKPTPK LTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH CUL1; SKP1A/ASK1 AND SPK1B/ASK2.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023041 Genomic DNA. Translation: BAB01063.1. CP002686 Genomic DNA. Translation: AEE77100.1. AY058077 mRNA. Translation: AAL24185.1. AY140009 mRNA. Translation: AAM98151.1. BT015945 mRNA. Translation: AAV34775.1.
IPI	IPI00529135.
RefSeq	NP_566787.1. NM_113504.2.
UniGene	At.23470. At.37291.
3D structure databases
ProteinModelPortal	Q9LU91.
SMR	Q9LU91. Positions 198-230.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LU91. 8 interactions.
STRING	Q9LU91.
Proteomic databases
PRIDE	Q9LU91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G26000.1; AT3G26000.1; AT3G26000.
GeneID	822197.
GenomeReviews	Gene locus AT3G26000 in contig BA000014_GR.
KEGG	ath:AT3G26000.
NMPDR	fig|3702.1.peg.14863.
Organism-specific databases
TAIR	At3g26000.
Phylogenomic databases
GeneTree	EPGT00050000018293.
InParanoid	Q9LU91.
OMA	ALDIEMC.
PhylomeDB	Q9LU91.
ProtClustDB	CLSN2687660.
Gene expression databases
Genevestigator	Q9LU91.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI14_ARATH

Accession

Primary (citable) accession number: Q9LU91
Secondary accession number(s): Q8L720

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program