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Protein

Calcium-transporting ATPase 9, plasma membrane-type

Gene

ACA9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei495 – 49514-aspartylphosphate intermediateBy similarity
Metal bindingi802 – 8021MagnesiumBy similarity
Metal bindingi806 – 8061MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • calmodulin binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • pollen development Source: TAIR
  • single fertilization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G21180-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.14. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 9, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 9
Gene namesi
Name:ACA9
Ordered Locus Names:At3g21180
ORF Names:MXL8.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G21180.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 194194CytoplasmicSequence analysisAdd
BLAST
Transmembranei195 – 21521HelicalSequence analysisAdd
BLAST
Topological domaini216 – 23318LumenalSequence analysisAdd
BLAST
Transmembranei234 – 25421HelicalSequence analysisAdd
BLAST
Topological domaini255 – 382128CytoplasmicSequence analysisAdd
BLAST
Transmembranei383 – 40220HelicalSequence analysisAdd
BLAST
Topological domaini403 – 43937LumenalSequence analysisAdd
BLAST
Transmembranei440 – 45718HelicalSequence analysisAdd
BLAST
Topological domaini458 – 857400CytoplasmicSequence analysisAdd
BLAST
Transmembranei858 – 87619HelicalSequence analysisAdd
BLAST
Topological domaini877 – 88711LumenalSequence analysisAdd
BLAST
Transmembranei888 – 90821HelicalSequence analysisAdd
BLAST
Topological domaini909 – 92820CytoplasmicSequence analysisAdd
BLAST
Transmembranei929 – 95123HelicalSequence analysisAdd
BLAST
Topological domaini952 – 96312LumenalSequence analysisAdd
BLAST
Transmembranei964 – 98825HelicalSequence analysisAdd
BLAST
Topological domaini989 – 100618CytoplasmicSequence analysisAdd
BLAST
Transmembranei1007 – 102822HelicalSequence analysisAdd
BLAST
Topological domaini1029 – 103810LumenalSequence analysis
Transmembranei1039 – 106022HelicalSequence analysisAdd
BLAST
Topological domaini1061 – 108626CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Calcium-transporting ATPase 9, plasma membrane-typePRO_0000046415Add
BLAST

Proteomic databases

PaxDbiQ9LU41.
PRIDEiQ9LU41.

PTM databases

iPTMnetiQ9LU41.

Expressioni

Gene expression databases

GenevisibleiQ9LU41. AT.

Interactioni

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

STRINGi3702.AT3G21180.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LU41.
SMRiQ9LU41. Positions 53-106, 151-930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 6812Interaction with calmodulinBy similarityAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LU41.
KOiK01537.
OMAiHENHAHA.
PhylomeDBiQ9LU41.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LU41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSSSNGLL LTSMSGRHDD MEAGSAKTEE HSDHEELQHD PDDPFDIDNT
60 70 80 90 100
KNASVESLRR WRQAALVLNA SRRFRYTLDL NKEEHYDNRR RMIRAHAQVI
110 120 130 140 150
RAALLFKLAG EQQIAFGSST PAASTGNFDI DLEKLVSMTR NQNMSNLQQY
160 170 180 190 200
GGVKGVAEKL KSNMEQGINE DEKEVIDRKN AFGSNTYPKK KGKNFFMFLW
210 220 230 240 250
EAWQDLTLII LIIAAVTSLA LGIKTEGLKE GWLDGGSIAF AVLLVIVVTA
260 270 280 290 300
VSDYRQSLQF QNLNDEKRNI QLEVMRGGRT VKISIYDVVV GDVIPLRIGD
310 320 330 340 350
QVPADGVLIS GHSLAIDESS MTGESKIVHK DQKSPFLMSG CKVADGVGNM
360 370 380 390 400
LVTGVGINTE WGLLMASISE DTGEETPLQV RLNGLATFIG IVGLSVALVV
410 420 430 440 450
LVALLVRYFT GTTQDTNGAT QFIKGTTSIS DIVDDCVKIF TIAVTIVVVA
460 470 480 490 500
VPEGLPLAVT LTLAYSMRKM MADKALVRRL SACETMGSAT TICSDKTGTL
510 520 530 540 550
TLNQMTVVET YAGGSKMDVA DNPSGLHPKL VALISEGVAQ NTTGNIFHPK
560 570 580 590 600
DGGEVEISGS PTEKAILSWA YKLGMKFDTI RSESAIIHAF PFNSEKKRGG
610 620 630 640 650
VAVLRGDSEV FIHWKGAAEI VLACCTQYMD SNGTLQSIES QKEFFRVAID
660 670 680 690 700
SMAKNSLRCV AIACRTQELN QVPKEQEDLD KWALPEDELI LLAIVGIKDP
710 720 730 740 750
CRPGVREAVR ICTSAGVKVR MVTGDNLQTA KAIALECGIL SSDTEAVEPT
760 770 780 790 800
IIEGKVFREL SEKEREQVAK KITVMGRSSP NDKLLLVQAL RKNGDVVAVT
810 820 830 840 850
GDGTNDAPAL HEADIGLSMG ISGTEVAKES SDIIILDDNF ASVVKVVRWG
860 870 880 890 900
RSVYANIQKF IQFQLTVNVA ALIINVVAAM SSGDVPLKAV QLLWVNLIMD
910 920 930 940 950
TLGALALATE PPTDHLMHRT PVGRREPLIT NIMWRNLLVQ SFYQVAVLLV
960 970 980 990 1000
LNFAGLSILG LNHENHAHAV EVKNTMIFNA FVMCQIFNEF NARKPDEMNV
1010 1020 1030 1040 1050
FRGVNKNPLF VAIVGVTFIL QIIIVTFLGK FAHTVRLGWQ LWLASIIIGL
1060 1070 1080
VSWPLAIVGK LIPVPKTPMS VYFKKPFRKY KASRNA
Length:1,086
Mass (Da):118,768
Last modified:June 26, 2007 - v2
Checksum:iEC317264EE1C3098
GO

Sequence cautioni

The sequence BAB01709.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023045 Genomic DNA. Translation: BAB01709.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE76473.1.
AK227228 mRNA. Translation: BAE99265.1.
RefSeqiNP_188755.2. NM_113013.4.
UniGeneiAt.38102.

Genome annotation databases

EnsemblPlantsiAT3G21180.1; AT3G21180.1; AT3G21180.
GeneIDi821671.
GrameneiAT3G21180.1; AT3G21180.1; AT3G21180.
KEGGiath:AT3G21180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023045 Genomic DNA. Translation: BAB01709.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE76473.1.
AK227228 mRNA. Translation: BAE99265.1.
RefSeqiNP_188755.2. NM_113013.4.
UniGeneiAt.38102.

3D structure databases

ProteinModelPortaliQ9LU41.
SMRiQ9LU41. Positions 53-106, 151-930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G21180.1.

Protein family/group databases

TCDBi3.A.3.2.14. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiQ9LU41.

Proteomic databases

PaxDbiQ9LU41.
PRIDEiQ9LU41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G21180.1; AT3G21180.1; AT3G21180.
GeneIDi821671.
GrameneiAT3G21180.1; AT3G21180.1; AT3G21180.
KEGGiath:AT3G21180.

Organism-specific databases

TAIRiAT3G21180.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LU41.
KOiK01537.
OMAiHENHAHA.
PhylomeDBiQ9LU41.

Enzyme and pathway databases

BioCyciARA:AT3G21180-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9LU41.

Gene expression databases

GenevisibleiQ9LU41. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiACA9_ARATH
AccessioniPrimary (citable) accession number: Q9LU41
Secondary accession number(s): Q0WUD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 26, 2007
Last modified: April 13, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.