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Protein

Protease Do-like 8, chloroplastic

Gene

DEGP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine protease.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Charge relay systemSequence analysis
Active sitei214 – 2141Charge relay systemSequence analysis
Active sitei292 – 2921Charge relay systemSequence analysis

GO - Molecular functioni

  • peptidase activity Source: TAIR
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • photosystem II repair Source: TAIR
  • proteolysis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciARA:GQT-1789-MONOMER.
ARA:GQT-337-MONOMER.

Protein family/group databases

MEROPSiS01.474.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 8, chloroplastic (EC:3.4.21.-)
Gene namesi
Name:DEGP8
Ordered Locus Names:At5g39830
ORF Names:K13H13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G39830.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid lumen Source: TAIR
  • thylakoid Source: TAIR
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei? – 90Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
Chaini91 – 448358Protease Do-like 8, chloroplasticPRO_0000026942Add
BLAST

Proteomic databases

PaxDbiQ9LU10.
PRIDEiQ9LU10.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LU10. baseline and differential.
GenevisibleiQ9LU10. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G39830.1.

Structurei

Secondary structure

1
448
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi114 – 12714Combined sources
Beta strandi130 – 1356Combined sources
Beta strandi155 – 1595Combined sources
Beta strandi165 – 1684Combined sources
Helixi170 – 1734Combined sources
Helixi174 – 1785Combined sources
Beta strandi186 – 1938Combined sources
Beta strandi199 – 21012Combined sources
Turni211 – 2144Combined sources
Beta strandi215 – 2195Combined sources
Turni224 – 2263Combined sources
Helixi235 – 2373Combined sources
Beta strandi243 – 2486Combined sources
Beta strandi250 – 2534Combined sources
Beta strandi256 – 27116Combined sources
Beta strandi274 – 28512Combined sources
Turni289 – 2935Combined sources
Beta strandi294 – 2985Combined sources
Beta strandi303 – 3075Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi321 – 3255Combined sources
Helixi326 – 33914Combined sources
Helixi354 – 3596Combined sources
Beta strandi364 – 3707Combined sources
Helixi376 – 3805Combined sources
Beta strandi398 – 4025Combined sources
Helixi410 – 4178Combined sources
Beta strandi425 – 4328Combined sources
Beta strandi435 – 4428Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IC6X-ray2.00A/B/C91-448[»]
ProteinModelPortaliQ9LU10.
SMRiQ9LU10. Positions 104-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini336 – 43398PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 333182Serine proteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiQ9LU10.
OMAiIQFGKVR.
PhylomeDBiQ9LU10.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LU10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVIASFCSK PNENEFVGRR QLLSSVCSKI SQGDVVSHPP VSSVKVTQDW
60 70 80 90 100
KSNLHELAVK SVPSTTRRIL LTSLFMNLCF NPSRYLSALA LGDPSVATVE
110 120 130 140 150
DVSPTVFPAG PLFPTEGRIV QLFEKNTYSV VNIFDVTLRP QLKMTGVVEI
160 170 180 190 200
PEGNGSGVVW DGQGYIVTNY HVIGNALSRN PSPGDVVGRV NILASDGVQK
210 220 230 240 250
NFEGKLVGAD RAKDLAVLKV DAPETLLKPI KVGQSNSLKV GQQCLAIGNP
260 270 280 290 300
FGFDHTLTVG VISGLNRDIF SQTGVTIGGG IQTDAAINPG NSGGPLLDSK
310 320 330 340 350
GNLIGINTAI FTQTGTSAGV GFAIPSSTVL KIVPQLIQFS KVLRAGINIE
360 370 380 390 400
LAPDPVANQL NVRNGALVLQ VPGKSLAEKA GLHPTSRGFA GNIVLGDIIV
410 420 430 440
AVDDKPVKNK AELMKILDEY SVGDKVTLKI KRGNEDLELK ISLEEKSS
Length:448
Mass (Da):47,493
Last modified:October 1, 2000 - v1
Checksum:iA986FC1387670AFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024023 Genomic DNA. Translation: BAA98101.1.
CP002688 Genomic DNA. Translation: AED94480.1.
AY056381 mRNA. Translation: AAL08237.1.
AY090319 mRNA. Translation: AAL90980.1.
RefSeqiNP_568575.1. NM_123346.4. [Q9LU10-1]
UniGeneiAt.22069.

Genome annotation databases

EnsemblPlantsiAT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
GeneIDi833979.
KEGGiath:AT5G39830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024023 Genomic DNA. Translation: BAA98101.1.
CP002688 Genomic DNA. Translation: AED94480.1.
AY056381 mRNA. Translation: AAL08237.1.
AY090319 mRNA. Translation: AAL90980.1.
RefSeqiNP_568575.1. NM_123346.4. [Q9LU10-1]
UniGeneiAt.22069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IC6X-ray2.00A/B/C91-448[»]
ProteinModelPortaliQ9LU10.
SMRiQ9LU10. Positions 104-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G39830.1.

Protein family/group databases

MEROPSiS01.474.

Proteomic databases

PaxDbiQ9LU10.
PRIDEiQ9LU10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
GeneIDi833979.
KEGGiath:AT5G39830.

Organism-specific databases

TAIRiAT5G39830.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiQ9LU10.
OMAiIQFGKVR.
PhylomeDBiQ9LU10.

Enzyme and pathway databases

BioCyciARA:GQT-1789-MONOMER.
ARA:GQT-337-MONOMER.

Miscellaneous databases

PROiQ9LU10.

Gene expression databases

ExpressionAtlasiQ9LU10. baseline and differential.
GenevisibleiQ9LU10. AT.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: PROTEIN SEQUENCE OF 91-113, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  5. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiDEGP8_ARATH
AccessioniPrimary (citable) accession number: Q9LU10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.