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Protein

Protease Do-like 8, chloroplastic

Gene

DEGP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable serine protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Charge relay systemSequence analysis1
Active sitei214Charge relay systemSequence analysis1
Active sitei292Charge relay systemSequence analysis1

GO - Molecular functioni

  • peptidase activity Source: TAIR
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • photosystem II repair Source: TAIR
  • proteolysis Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.474

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 8, chloroplastic (EC:3.4.21.-)
Gene namesi
Name:DEGP8
Ordered Locus Names:At5g39830
ORF Names:K13H13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G39830
TAIRilocus:2151916 AT5G39830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 90Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000002694291 – 448Protease Do-like 8, chloroplasticAdd BLAST358

Proteomic databases

PaxDbiQ9LU10

Expressioni

Gene expression databases

ExpressionAtlasiQ9LU10 baseline and differential
GenevisibleiQ9LU10 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G39830.1

Structurei

Secondary structure

1448
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi114 – 127Combined sources14
Beta strandi130 – 135Combined sources6
Beta strandi155 – 159Combined sources5
Beta strandi165 – 168Combined sources4
Helixi170 – 173Combined sources4
Helixi174 – 178Combined sources5
Beta strandi186 – 193Combined sources8
Beta strandi199 – 210Combined sources12
Turni211 – 214Combined sources4
Beta strandi215 – 219Combined sources5
Turni224 – 226Combined sources3
Helixi235 – 237Combined sources3
Beta strandi243 – 248Combined sources6
Beta strandi250 – 253Combined sources4
Beta strandi256 – 271Combined sources16
Beta strandi274 – 285Combined sources12
Turni289 – 293Combined sources5
Beta strandi294 – 298Combined sources5
Beta strandi303 – 307Combined sources5
Beta strandi313 – 315Combined sources3
Beta strandi321 – 325Combined sources5
Helixi326 – 339Combined sources14
Helixi354 – 359Combined sources6
Beta strandi364 – 370Combined sources7
Helixi376 – 380Combined sources5
Beta strandi398 – 402Combined sources5
Helixi410 – 417Combined sources8
Beta strandi425 – 432Combined sources8
Beta strandi435 – 442Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IC6X-ray2.00A/B/C91-448[»]
ProteinModelPortaliQ9LU10
SMRiQ9LU10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini336 – 433PDZAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 333Serine proteaseAdd BLAST182

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320 Eukaryota
COG0265 LUCA
HOGENOMiHOG000223641
InParanoidiQ9LU10
OMAiCFCLHSS
OrthoDBiEOG09360AWI
PhylomeDBiQ9LU10

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR009003 Peptidase_S1_PA
IPR001940 Peptidase_S1C
PfamiView protein in Pfam
PF13180 PDZ_2, 1 hit
PRINTSiPR00834 PROTEASES2C
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF50494 SSF50494, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LU10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVIASFCSK PNENEFVGRR QLLSSVCSKI SQGDVVSHPP VSSVKVTQDW
60 70 80 90 100
KSNLHELAVK SVPSTTRRIL LTSLFMNLCF NPSRYLSALA LGDPSVATVE
110 120 130 140 150
DVSPTVFPAG PLFPTEGRIV QLFEKNTYSV VNIFDVTLRP QLKMTGVVEI
160 170 180 190 200
PEGNGSGVVW DGQGYIVTNY HVIGNALSRN PSPGDVVGRV NILASDGVQK
210 220 230 240 250
NFEGKLVGAD RAKDLAVLKV DAPETLLKPI KVGQSNSLKV GQQCLAIGNP
260 270 280 290 300
FGFDHTLTVG VISGLNRDIF SQTGVTIGGG IQTDAAINPG NSGGPLLDSK
310 320 330 340 350
GNLIGINTAI FTQTGTSAGV GFAIPSSTVL KIVPQLIQFS KVLRAGINIE
360 370 380 390 400
LAPDPVANQL NVRNGALVLQ VPGKSLAEKA GLHPTSRGFA GNIVLGDIIV
410 420 430 440
AVDDKPVKNK AELMKILDEY SVGDKVTLKI KRGNEDLELK ISLEEKSS
Length:448
Mass (Da):47,493
Last modified:October 1, 2000 - v1
Checksum:iA986FC1387670AFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024023 Genomic DNA Translation: BAA98101.1
CP002688 Genomic DNA Translation: AED94480.1
AY056381 mRNA Translation: AAL08237.1
AY090319 mRNA Translation: AAL90980.1
RefSeqiNP_568575.1, NM_123346.5 [Q9LU10-1]
UniGeneiAt.22069

Genome annotation databases

EnsemblPlantsiAT5G39830.1; AT5G39830.1; AT5G39830 [Q9LU10-1]
GeneIDi833979
GrameneiAT5G39830.1; AT5G39830.1; AT5G39830 [Q9LU10-1]
KEGGiath:AT5G39830

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDEGP8_ARATH
AccessioniPrimary (citable) accession number: Q9LU10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome