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Protein

Protease Do-like 8, chloroplastic

Gene

DEGP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Charge relay systemSequence analysis1
Active sitei214Charge relay systemSequence analysis1
Active sitei292Charge relay systemSequence analysis1

GO - Molecular functioni

  • peptidase activity Source: TAIR
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • photosystem II repair Source: TAIR
  • proteolysis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciARA:GQT-1789-MONOMER.

Protein family/group databases

MEROPSiS01.474.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 8, chloroplastic (EC:3.4.21.-)
Gene namesi
Name:DEGP8
Ordered Locus Names:At5g39830
ORF Names:K13H13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G39830.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid lumen Source: TAIR
  • thylakoid Source: TAIR
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 90Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000002694291 – 448Protease Do-like 8, chloroplasticAdd BLAST358

Proteomic databases

PaxDbiQ9LU10.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LU10. baseline and differential.
GenevisibleiQ9LU10. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G39830.1.

Structurei

Secondary structure

1448
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi114 – 127Combined sources14
Beta strandi130 – 135Combined sources6
Beta strandi155 – 159Combined sources5
Beta strandi165 – 168Combined sources4
Helixi170 – 173Combined sources4
Helixi174 – 178Combined sources5
Beta strandi186 – 193Combined sources8
Beta strandi199 – 210Combined sources12
Turni211 – 214Combined sources4
Beta strandi215 – 219Combined sources5
Turni224 – 226Combined sources3
Helixi235 – 237Combined sources3
Beta strandi243 – 248Combined sources6
Beta strandi250 – 253Combined sources4
Beta strandi256 – 271Combined sources16
Beta strandi274 – 285Combined sources12
Turni289 – 293Combined sources5
Beta strandi294 – 298Combined sources5
Beta strandi303 – 307Combined sources5
Beta strandi313 – 315Combined sources3
Beta strandi321 – 325Combined sources5
Helixi326 – 339Combined sources14
Helixi354 – 359Combined sources6
Beta strandi364 – 370Combined sources7
Helixi376 – 380Combined sources5
Beta strandi398 – 402Combined sources5
Helixi410 – 417Combined sources8
Beta strandi425 – 432Combined sources8
Beta strandi435 – 442Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IC6X-ray2.00A/B/C91-448[»]
ProteinModelPortaliQ9LU10.
SMRiQ9LU10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini336 – 433PDZAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 333Serine proteaseAdd BLAST182

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiQ9LU10.
OMAiIQFGKVR.
OrthoDBiEOG09360AWI.
PhylomeDBiQ9LU10.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LU10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVIASFCSK PNENEFVGRR QLLSSVCSKI SQGDVVSHPP VSSVKVTQDW
60 70 80 90 100
KSNLHELAVK SVPSTTRRIL LTSLFMNLCF NPSRYLSALA LGDPSVATVE
110 120 130 140 150
DVSPTVFPAG PLFPTEGRIV QLFEKNTYSV VNIFDVTLRP QLKMTGVVEI
160 170 180 190 200
PEGNGSGVVW DGQGYIVTNY HVIGNALSRN PSPGDVVGRV NILASDGVQK
210 220 230 240 250
NFEGKLVGAD RAKDLAVLKV DAPETLLKPI KVGQSNSLKV GQQCLAIGNP
260 270 280 290 300
FGFDHTLTVG VISGLNRDIF SQTGVTIGGG IQTDAAINPG NSGGPLLDSK
310 320 330 340 350
GNLIGINTAI FTQTGTSAGV GFAIPSSTVL KIVPQLIQFS KVLRAGINIE
360 370 380 390 400
LAPDPVANQL NVRNGALVLQ VPGKSLAEKA GLHPTSRGFA GNIVLGDIIV
410 420 430 440
AVDDKPVKNK AELMKILDEY SVGDKVTLKI KRGNEDLELK ISLEEKSS
Length:448
Mass (Da):47,493
Last modified:October 1, 2000 - v1
Checksum:iA986FC1387670AFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024023 Genomic DNA. Translation: BAA98101.1.
CP002688 Genomic DNA. Translation: AED94480.1.
AY056381 mRNA. Translation: AAL08237.1.
AY090319 mRNA. Translation: AAL90980.1.
RefSeqiNP_568575.1. NM_123346.5. [Q9LU10-1]
UniGeneiAt.22069.

Genome annotation databases

EnsemblPlantsiAT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
GeneIDi833979.
GrameneiAT5G39830.1; AT5G39830.1; AT5G39830.
KEGGiath:AT5G39830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024023 Genomic DNA. Translation: BAA98101.1.
CP002688 Genomic DNA. Translation: AED94480.1.
AY056381 mRNA. Translation: AAL08237.1.
AY090319 mRNA. Translation: AAL90980.1.
RefSeqiNP_568575.1. NM_123346.5. [Q9LU10-1]
UniGeneiAt.22069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IC6X-ray2.00A/B/C91-448[»]
ProteinModelPortaliQ9LU10.
SMRiQ9LU10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G39830.1.

Protein family/group databases

MEROPSiS01.474.

Proteomic databases

PaxDbiQ9LU10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
GeneIDi833979.
GrameneiAT5G39830.1; AT5G39830.1; AT5G39830.
KEGGiath:AT5G39830.

Organism-specific databases

TAIRiAT5G39830.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiQ9LU10.
OMAiIQFGKVR.
OrthoDBiEOG09360AWI.
PhylomeDBiQ9LU10.

Enzyme and pathway databases

BioCyciARA:GQT-1789-MONOMER.

Miscellaneous databases

PROiQ9LU10.

Gene expression databases

ExpressionAtlasiQ9LU10. baseline and differential.
GenevisibleiQ9LU10. AT.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDEGP8_ARATH
AccessioniPrimary (citable) accession number: Q9LU10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.