Q9LU10 (DEGP8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease Do-like 8, chloroplastic
EC=3.4.21.-

Gene names

Name:	DEGP8
Ordered Locus Names:	At5g39830
ORF Names:	K13H13.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable serine protease.
Subcellular location	Plastid › chloroplast thylakoid lumen Probable.
Sequence similarities	Belongs to the peptidase S1B family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid Thylakoid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	photosystem II repair Inferred from mutant phenotype PubMed 17449806. Source: TAIR proteolysis Inferred from direct assay PubMed 17449806. Source: TAIR
Cellular_component	chloroplast thylakoid lumen Inferred from direct assay PubMed 17449806. Source: TAIR
Molecular_function	peptidase activity Inferred from direct assay PubMed 17449806. Source: TAIR serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LU10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Transit peptide	? – 90		Thylakoid Ref.4
	Chain	91 – 448	358	Protease Do-like 8, chloroplastic		PRO_0000026942
Regions
	Domain	336 – 433	98	PDZ
	Region	152 – 333	182	Serine protease
Sites
	Active site	171	1	Charge relay system Potential
	Active site	214	1	Charge relay system Potential
	Active site	292	1	Charge relay system Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A986FC1387670AFF	FASTA	448	47,493
	10 20 30 40 50 60 MQVIASFCSK PNENEFVGRR QLLSSVCSKI SQGDVVSHPP VSSVKVTQDW KSNLHELAVK 70 80 90 100 110 120 SVPSTTRRIL LTSLFMNLCF NPSRYLSALA LGDPSVATVE DVSPTVFPAG PLFPTEGRIV 130 140 150 160 170 180 QLFEKNTYSV VNIFDVTLRP QLKMTGVVEI PEGNGSGVVW DGQGYIVTNY HVIGNALSRN 190 200 210 220 230 240 PSPGDVVGRV NILASDGVQK NFEGKLVGAD RAKDLAVLKV DAPETLLKPI KVGQSNSLKV 250 260 270 280 290 300 GQQCLAIGNP FGFDHTLTVG VISGLNRDIF SQTGVTIGGG IQTDAAINPG NSGGPLLDSK 310 320 330 340 350 360 GNLIGINTAI FTQTGTSAGV GFAIPSSTVL KIVPQLIQFS KVLRAGINIE LAPDPVANQL 370 380 390 400 410 420 NVRNGALVLQ VPGKSLAEKA GLHPTSRGFA GNIVLGDIIV AVDDKPVKNK AELMKILDEY 430 440 SVGDKVTLKI KRGNEDLELK ISLEEKSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Proteome map of the chloroplast lumen of Arabidopsis thaliana." Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P., Kieselbach T. J. Biol. Chem. 277:8354-8365(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 91-113 AND 345-361. Strain: cv. Columbia.
[5]	"Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature." Adam Z., Adamska I., Nakabayashi K., Ostersetzer O., Haussuhl K., Manuell A., Zheng B., Vallon O., Rodermel S.R., Shinozaki K., Clarke A.K. Plant Physiol. 125:1912-1918(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024023 Genomic DNA. Translation: BAA98101.1. CP002688 Genomic DNA. Translation: AED94480.1. AY056381 mRNA. Translation: AAL08237.1. AY090319 mRNA. Translation: AAL90980.1.
IPI	IPI00517605.
RefSeq	NP_568575.1. NM_123346.4.
UniGene	At.22069.
3D structure databases
ProteinModelPortal	Q9LU10.
SMR	Q9LU10. Positions 120-446.
ModBase	Search...
Protein family/group databases
MEROPS	S01.474.
Proteomic databases
PaxDb	Q9LU10.
PRIDE	Q9LU10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G39830.1; AT5G39830.1; AT5G39830.
GeneID	833979.
KEGG	ath:AT5G39830.
Organism-specific databases
GeneFarm	2272. 200.
TAIR	At5g39830.
Phylogenomic databases
eggNOG	COG0265.
HOGENOM	HOG000223641.
InParanoid	Q9LU10.
KO	K01362.
OMA	HMIGINT.
PhylomeDB	Q9LU10.
ProtClustDB	CLSN2689900.
Gene expression databases
ArrayExpress	Q9LU10.
Genevestigator	Q9LU10.
Family and domain databases
InterPro	IPR001478. PDZ. IPR001940. Peptidase_S1C. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF13180. PDZ_2. 1 hit. [Graphical view]
PRINTS	PR00834. PROTEASES2C.
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEGP8_ARATH

Accession

Primary (citable) accession number: Q9LU10

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program