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Protein

Heat shock 70 kDa protein 7, chloroplastic

Gene

HSP70-7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts redundantly with HSP70-6 in the thermotolerance of germinating seeds. Plays an important role in the protein precursor import into chloroplasts.3 Publications
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • protein folding Source: InterPro
  • protein targeting to chloroplast Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to heat Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Stress response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 7, chloroplastic
Alternative name(s):
Chloroplast heat shock protein 70-2
Short name:
cpHsc70-2
Heat shock protein 70-7
Short name:
AtHsp70-7
Gene namesi
Name:HSP70-7
Synonyms:CPHSC70-2, HSC70-7
Ordered Locus Names:At5g49910
ORF Names:K9P8.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G49910.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • plastid stroma Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Defective in protein import into chloroplasts during early developmental stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 9292ChloroplastSequence analysisAdd
BLAST
Chaini93 – 718626Heat shock 70 kDa protein 7, chloroplasticPRO_0000415426Add
BLAST

Proteomic databases

PaxDbiQ9LTX9.
PRIDEiQ9LTX9.

2D gel databases

World-2DPAGE0003:Q9LTX9.

PTM databases

iPTMnetiQ9LTX9.

Expressioni

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevisibleiQ9LTX9. AT.

Interactioni

Protein-protein interaction databases

BioGridi20300. 1 interaction.
IntActiQ9LTX9. 1 interaction.
STRINGi3702.AT5G49910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LTX9.
SMRiQ9LTX9. Positions 79-673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0102. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ9LTX9.
KOiK03283.
OMAiSVAYQVE.
PhylomeDBiQ9LTX9.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LTX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSAAQIHI LGGIGFPTSS SSSSTKNLDN KTNSIPRSVF FGNRTSPFTT
60 70 80 90 100
PTSAFLRMGR RNNNASRYTV GPVRVVNEKV VGIDLGTTNS AVAAMEGGKP
110 120 130 140 150
TIVTNAEGQR TTPSVVAYTK SKDRLVGQIA KRQAVVNPEN TFFSVKRFIG
160 170 180 190 200
RRMNEVAEES KQVSYRVIKD ENGNVKLDCP AIGKQFAAEE ISAQVLRKLV
210 220 230 240 250
DDASRFLNDK VTKAVITVPA YFNDSQRTAT KDAGRIAGLE VLRIINEPTA
260 270 280 290 300
ASLAYGFERK SNETILVFDL GGGTFDVSVL EVGDGVFEVL STSGDTHLGG
310 320 330 340 350
DDFDKRVVDW LASTFKKDEG IDLLKDKQAL QRLTEAAEKA KIELSSLTQT
360 370 380 390 400
NMSLPFITAT ADGPKHIETT LTRGKFEELC SDLLDRVRTP VENSLRDAKL
410 420 430 440 450
SFKDIDEVIL VGGSTRIPAV QDLVRKLTGK EPNVSVNPDE VVALGAAVQA
460 470 480 490 500
GVLSGDVSDI VLLDVTPLSL GLETLGGVMT KIIPRNTTLP TSKSEVFSTA
510 520 530 540 550
ADGQTSVEIN VLQGEREFVR DNKSIGSFRL DGIPPAPRGV PQIEVKFDID
560 570 580 590 600
ANGILSVSAS DKGTGKKQDI TITGASTLPK DEVDTMVQEA ERFAKEDKEK
610 620 630 640 650
RDAIDTKNQA DSVVYQTEKQ LKELGEKIPG PVKEKVEAKL QELKEKIASG
660 670 680 690 700
STQEIKDTMA ALNQEVMQIG QSLYNQPQPG GADSPPGGEA SSSSDTSSSA
710
KGGDNGGDVI DADFTDSN
Length:718
Mass (Da):76,997
Last modified:October 1, 2000 - v1
Checksum:iFFD96CC29E3A463B
GO
Isoform 2 (identifier: Q9LTX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-371: SLPFITATADGPKHIETTL → RFTFPSQIYLPRSHWYVGY
     372-718: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:371
Mass (Da):40,539
Checksum:iBDEB1F7F9A8F2ED7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 214TSSS → NSFC in AAF27639 (PubMed:11402207).Curated
Sequence conflicti49 – 491T → S in AAF27639 (PubMed:11402207).Curated
Sequence conflicti400 – 4001L → F in AAF27639 (PubMed:11402207).Curated
Sequence conflicti562 – 5621K → Q in AAF27639 (PubMed:11402207).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei353 – 37119SLPFI…IETTL → RFTFPSQIYLPRSHWYVGY in isoform 2. 1 PublicationVSP_042242Add
BLAST
Alternative sequencei372 – 718347Missing in isoform 2. 1 PublicationVSP_042243Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217459 mRNA. Translation: AAF27639.1.
AB024032 Genomic DNA. Translation: BAA97012.1.
CP002688 Genomic DNA. Translation: AED95870.1.
BT000919 mRNA. Translation: AAN41319.1.
AY081331 mRNA. Translation: AAL91220.1.
BT008452 mRNA. Translation: AAP37811.1.
RefSeqiNP_199802.1. NM_124369.3. [Q9LTX9-1]
UniGeneiAt.21829.
At.70293.

Genome annotation databases

EnsemblPlantsiAT5G49910.1; AT5G49910.1; AT5G49910. [Q9LTX9-1]
GeneIDi835054.
KEGGiath:AT5G49910.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217459 mRNA. Translation: AAF27639.1.
AB024032 Genomic DNA. Translation: BAA97012.1.
CP002688 Genomic DNA. Translation: AED95870.1.
BT000919 mRNA. Translation: AAN41319.1.
AY081331 mRNA. Translation: AAL91220.1.
BT008452 mRNA. Translation: AAP37811.1.
RefSeqiNP_199802.1. NM_124369.3. [Q9LTX9-1]
UniGeneiAt.21829.
At.70293.

3D structure databases

ProteinModelPortaliQ9LTX9.
SMRiQ9LTX9. Positions 79-673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20300. 1 interaction.
IntActiQ9LTX9. 1 interaction.
STRINGi3702.AT5G49910.1.

PTM databases

iPTMnetiQ9LTX9.

2D gel databases

World-2DPAGE0003:Q9LTX9.

Proteomic databases

PaxDbiQ9LTX9.
PRIDEiQ9LTX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G49910.1; AT5G49910.1; AT5G49910. [Q9LTX9-1]
GeneIDi835054.
KEGGiath:AT5G49910.

Organism-specific databases

TAIRiAT5G49910.

Phylogenomic databases

eggNOGiKOG0102. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ9LTX9.
KOiK03283.
OMAiSVAYQVE.
PhylomeDBiQ9LTX9.

Miscellaneous databases

PROiQ9LTX9.

Gene expression databases

GenevisibleiQ9LTX9. AT.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
    Sung D.Y., Vierling E., Guy C.L.
    Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.
  5. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
    Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
    Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts."
    Ratnayake R.M., Inoue H., Nonami H., Akita M.
    Biosci. Biotechnol. Biochem. 72:2926-2935(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Arabidopsis stromal 70-kD heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds."
    Su P.H., Li H.M.
    Plant Physiol. 146:1231-1241(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development."
    Latijnhouwers M., Xu X.M., Moeller S.G.
    Planta 232:567-578(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts."
    Su P.H., Li H.M.
    Plant Cell 22:1516-1531(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiHSP7G_ARATH
AccessioniPrimary (citable) accession number: Q9LTX9
Secondary accession number(s): Q8RXD1, Q9M637
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: October 1, 2000
Last modified: January 20, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.