Q9LTR9 (NMT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase 1
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase 1
Short name=NMT 1
Short name=Type I N-myristoyltransferase 1
Peptide N-myristoyltransferase 1

Gene names

Name:	NMT1
Ordered Locus Names:	At5g57020
ORF Names:	MHM17.15

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins. Can also use decanoyl-CoA and lauroyl-CoA as substrates. Ref.1 Ref.2
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subcellular location	Cytoplasm. Note: Mostly associated to ribosomes. Present to a lower extent in mitochondria. Ref.1
Tissue specificity	Expressed ubiquitously, with higher levels in young tissues (at protein level). Ref.1
Sequence similarities	Belongs to the NMT family.
Biophysicochemical properties	Kinetic parameters: KM=3.7 µM for myristoyl-CoA, in the presence of CPK3 Ref.1 pH dependence: Optimum pH is 7.8. Active from pH 5.5 to 8.5.
Sequence caution	The sequence BAA97032.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from direct assay Ref.1. Source: TAIR embryonic shoot morphogenesis Inferred from mutant phenotype. Source: TAIR growth Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR ribosome Inferred from direct assay Ref.1. Source: TAIR
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Glycylpeptide N-tetradecanoyltransferase 1		PRO_0000064231
Regions
	Region	48 – 51	4	Myristoyl CoA-binding By similarity
	Region	181 – 217	37	Myristoyl CoA-binding By similarity
Sites
	Active site	434	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LTR9 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 778138CBF91525D4	FASTA	434	49,799
	10 20 30 40 50 60 MADNNSPPGS VEQKADQIVE ANPLVKDDTS LETIVRRFQD SMSEAKTHKF WETQPVGQFK 70 80 90 100 110 120 DIGDTSLPEG PIEPATPLSE VKQEPYNLPS VYEWTTCDMN SDDMCSEVYN LLKNNYVEDD 130 140 150 160 170 180 ENMFRFNYSK EFLRWALRPP GYYQSWHIGV RAKTSKKLVA FISGVPARIR VRDEVVKMAE 190 200 210 220 230 240 INFLCVHKKL RSKRLAPVMI KEVTRRVHLE NIWQAAYTAG VILPTPITTC QYWHRSLNPK 250 260 270 280 290 300 KLIDVGFSRL GARMTMSRTI KLYKLPDAPI TPGFRKMEPR DVPAVTRLLR NYLSQFGVAT 310 320 330 340 350 360 DFDENDVEHW LLPREDVVDS YLVESPETHD VTDFCSFYTL PSTILGNPNY TTLKAAYSYY 370 380 390 400 410 420 NVATQTSFLQ LMNDALIVSK QKGFDVFNAL DVMHNESFLK ELKFGPGDGQ LHYYLYNYRL 430 KSALKPAELG LVLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, genomic organization, and biochemical characterization of myristoyl-CoA:protein N-myristoyltransferase from Arabidopsis thaliana." Qi Q., Rajala R.V.S., Anderson W., Jiang C., Rozwadowski K., Selvaraj G., Sharma R., Datla R. J. Biol. Chem. 275:9673-9683(2000) [PubMed: 10734119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[2]	"Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote." Boisson B., Giglione C., Meinnel T. J. Biol. Chem. 278:43418-43429(2003) [PubMed: 12912986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF193616 mRNA. Translation: AAF60968.1. AF250956 mRNA. Translation: AAK49037.1. AB024035 Genomic DNA. Translation: BAA97032.1. Sequence problems. CP002688 Genomic DNA. Translation: AED96835.1. AY045854 mRNA. Translation: AAK76528.1. AY091380 mRNA. Translation: AAM14319.1. AY087547 mRNA. Translation: AAM65089.1.
IPI	IPI00517570.
RefSeq	NP_568846.1. NM_125084.2.
UniGene	At.25387.
3D structure databases
ProteinModelPortal	Q9LTR9.
SMR	Q9LTR9. Positions 46-433.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LTR9.
Proteomic databases
PRIDE	Q9LTR9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G57020.1; AT5G57020.1; AT5G57020.
GeneID	835805.
GenomeReviews	Gene locus AT5G57020 in contig BA000015_GR.
KEGG	ath:AT5G57020.
NMPDR	fig|3702.1.peg.27644.
Organism-specific databases
GeneFarm	5182.
TAIR	At5g57020.
Phylogenomic databases
GeneTree	EPGT00050000010543.
HOGENOM	HBG314670.
InParanoid	Q9LTR9.
OMA	EHAKGSP.
PhylomeDB	Q9LTR9.
ProtClustDB	CLSN2690031.
Gene expression databases
Genevestigator	Q9LTR9.
GermOnline	AT5G57020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
KO	K00671.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT1_ARATH

Accession

Primary (citable) accession number: Q9LTR9
Secondary accession number(s): Q9M6E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	November 16, 2011
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program