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Q9LTG5 (CALS4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 4
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 9
Gene names
Name:CALS4
Synonyms:GSL9
Ordered Locus Names:At5g36870
ORF Names:F5H8.14
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1871 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence BAA98065.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18711871Callose synthase 4
PRO_0000334576

Regions

Topological domain1 – 491491Cytoplasmic Potential
Transmembrane492 – 51221Helical; Potential
Topological domain513 – 5219Extracellular Potential
Transmembrane522 – 54221Helical; Potential
Topological domain543 – 55816Cytoplasmic Potential
Transmembrane559 – 57921Helical; Potential
Topological domain580 – 5834Extracellular Potential
Transmembrane584 – 60421Helical; Potential
Topological domain605 – 64036Cytoplasmic Potential
Transmembrane641 – 66121Helical; Potential
Topological domain662 – 70140Extracellular Potential
Transmembrane702 – 72423Helical; Potential
Topological domain725 – 1441717Cytoplasmic Potential
Transmembrane1442 – 146221Helical; Potential
Topological domain1463 – 148523Extracellular Potential
Transmembrane1486 – 150621Helical; Potential
Topological domain1507 – 151610Cytoplasmic Potential
Transmembrane1517 – 153721Helical; Potential
Topological domain1538 – 158043Extracellular Potential
Transmembrane1581 – 160121Helical; Potential
Transmembrane1602 – 162221Helical; Potential
Topological domain1623 – 167553Extracellular Potential
Transmembrane1676 – 169621Helical; Potential
Topological domain1697 – 170812Cytoplasmic Potential
Transmembrane1709 – 172921Helical; Potential
Topological domain1730 – 174112Extracellular Potential
Transmembrane1742 – 176221Helical; Potential
Topological domain1763 – 177210Cytoplasmic Potential
Transmembrane1773 – 179321Helical; Potential
Topological domain1794 – 181522Extracellular Potential
Transmembrane1816 – 183621Helical; Potential
Topological domain1837 – 187135Cytoplasmic Potential
Compositional bias146 – 1494Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
Q9LTG5 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 3BDD16A01AD5DA44

FASTA1,871216,138
        10         20         30         40         50         60 
MNQPNRGQIL QTVFSHFFPV ASPDSELVPS SLHEDITPIL RVAKDVEDTN PRSLFLQDLD 

        70         80         90        100        110        120 
IKSVDDSINI LSGHSHALDK ANELDPTSSG RDVRQFKNTI LQWLEKNNES TLKARQKSSD 

       130        140        150        160        170        180 
AHEMQSFYQQ YGDEGINDLL NAGAGSSSSQ RTKIYQTAVV LYDVLDAVHR KANIKVAAKI 

       190        200        210        220        230        240 
LESHAEVEAK NKIYVPYNIL PLDPDSKNHA MMRDPKIVAV LKAIRYTSDL TWQIGHKIND 

       250        260        270        280        290        300 
DEDVLDWLKT MFRFQKDNVS NQREHLILLL ANVQMRQTQR QPNLLDDRAL DTVMEKLLGN 

       310        320        330        340        350        360 
YNKWCNHVGL ESSLRFPKDK QQKVVQQRKL LYTGLYLLIW GEAANLRFMP ECLCYIYHHM 

       370        380        390        400        410        420 
AFELFEMLES KGSKKKYKPK NPTYSGKDED FLTKVVTPVY KTIAEEAKKS GEGKHSEWRN 

       430        440        450        460        470        480 
YDDLNEYFWS KQYLDKLGWP MKANADFFCK TSQQLGLNKS EKKPDLGDGC VGKVNFVEIR 

       490        500        510        520        530        540 
TFWHLFRSFD RMWSFYILSL QAMIIIAWNE TSESGGAVFH KVLSVFITAA KLNLFQAFLD 

       550        560        570        580        590        600 
IALSWKARHS MSTHVRQRYI FKAVAAAVWV LLMPLTYAYS HTSIFIVAIL IYLSPNMLPE 

       610        620        630        640        650        660 
MLLLIPSIRR TLEKSDFRPV KLIMWWSQPE LYIGRGMHES AWSIYKYMMF WIVLLTSKLA 

       670        680        690        700        710        720 
FSYYVEQIKP LMGPTKEIMS VPMPGYWLPE FFPHVKNNRG VVITLWSPVI LVYFMDTQIW 

       730        740        750        760        770        780 
YAIVSTLVGG LYGAFRHIGE IQTLGMLRSR FQSLPGAFNA CLIPNENTKE KGIKLAFSRK 

       790        800        810        820        830        840 
CHKIPNTNGK EAKQFSQMWN TIINSFREED LISNRELELL LMSCWAYPDL DFIRWPIFLL 

       850        860        870        880        890        900 
ASKIPIAVDI AKKRNGKHRE LKNILAEDNC MSCAVRECYA SIKKLLNTLV TGNSDLMLIT 

       910        920        930        940        950        960 
TVFTIIDTHI EKDTLLTELN LSVLPDLHGH FVKLTEYVLQ NKDKDKIQIV NVLLKILEMV 

       970        980        990       1000       1010       1020 
TKDILKEEIK RLHLLLTVKE SAMDVPSNLE ARRRLTFFSN SLFMEMPGAP KIQNMLSFSA 

      1030       1040       1050       1060       1070       1080 
LTPYYSEDVL FSTFDLEKEN DGVSILFYLQ KIFPDEWKNF LERVKCGTEE ELDAIDYLKE 

      1090       1100       1110       1120       1130       1140 
EIRLWASYRG QTLTKTVRGM MYYQKALELQ AFFDLANERE LMKGYKSAEA SSSGSSLWAE 

      1150       1160       1170       1180       1190       1200 
CQALADIKFT YVVACQQYSI HKRSGDQRAK DILTLMTTYP SLRVAYIDEV EQTHIYSKGT 

      1210       1220       1230       1240       1250       1260 
SENFYYSALV KAAPQTYSTD SSDSGHMLDQ VIYQIKLPGP PIIGEGKPEN QNNAIIFTRG 

      1270       1280       1290       1300       1310       1320 
EALQTIDMNQ DYYIEEAFKM RNLLQEFLEK NGGVRYPTIL GLREHIFTRS VSCLAWFMSN 

      1330       1340       1350       1360       1370       1380 
QEHSFVTIGQ RVLANPLKVR FHYGHPDVFD RVFHLTRGGV SKASKVINLS EDIFAGFNST 

      1390       1400       1410       1420       1430       1440 
LREGTVSHHE YIQVGKGRDV GLNQISMFEA KIANGSGEQT LSRDLYRLGH QFDFFRMLSC 

      1450       1460       1470       1480       1490       1500 
YFTTVGFYFC SMLTVLTVYV FLYGRLYLVL SGVEKELGNK PMMMEIILAS QSFVQIVFLM 

      1510       1520       1530       1540       1550       1560 
AMPMIMEIGL ERGFYDALFD FVLMQLQLAS VFFTFQLGTK FHYYCKTLLH GGAEYRGTGR 

      1570       1580       1590       1600       1610       1620 
GFVVFHAKFA ENYRFYSRSH FVKATELGIL LLVYHIFGPT YIGLFTISIW FMVGTWLFAP 

      1630       1640       1650       1660       1670       1680 
FLFNPSGFEW HEIVEDWADW KKWIEYDNGG IGVPPEKSWE SWWEKDIEHL QHSGKWGIVV 

      1690       1700       1710       1720       1730       1740 
EIFFALRFFI FQYGLVYQLS AFKNKYSSLW VFGASWLLIL ILLLTVTVLD YARRRLGTEF 

      1750       1760       1770       1780       1790       1800 
QLLFRIIKVS LFLAFMAIFI TLMTCRLILP QDVFLCMLAL IPTGWGLLLI AQSCKPLIQQ 

      1810       1820       1830       1840       1850       1860 
PGIWSWVMTL AWVYDLVMGS LLFIPIAFMA WFPFISEFQT RMLFNQAFSR GLHISRILSG 

      1870 
QRKHRSSKNK D

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[4]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB025605 Genomic DNA. Translation: BAA98065.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94119.1.
IPIIPI00535125.
RefSeqNP_198503.3. NM_123045.3.

3D structure databases

ProteinModelPortalQ9LTG5.
ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9LTG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G36870.1; AT5G36870.1; AT5G36870.
GeneID833654.
GenomeReviewsGene locus AT5G36870 in contig BA000015_GR.
KEGGath:AT5G36870.

Organism-specific databases

TAIRAt5g36870.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000029319.
HOGENOMHBG316390.
PhylomeDBQ9LTG5.

Gene expression databases

GenevestigatorQ9LTG5.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
KOK11000.
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS4_ARATH
AccessionPrimary (citable) accession number: Q9LTG5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents