Reviewed, UniProtKB/Swiss-Prot Q9LT08 (PSDE_ARATH)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 14
    EC=3.1.2.15
Alternative name(s):
    26S proteasome regulatory subunit rpn11

Gene names

Name:	RPN11
Ordered Locus Names:	At5g23540
ORF Names:	MQM1.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of the 19S regulatory cap of the 26S proteasome.
Sequence similarities	Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	nucleus Traceable author statement. Source: TAIR proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LT08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	26S proteasome non-ATPase regulatory subunit 14		PRO_0000213956
Regions
	Motif	112 – 125	14	JAMM motif
Sites
	Metal binding	112	1	Zinc; catalytic By similarity
	Metal binding	114	1	Zinc; catalytic By similarity
	Metal binding	125	1	Zinc; catalytic By similarity
Experimental info
	Sequence conflict	171	1	Q → H in AAM64349. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1AF64751FA29819A	FASTA	308	34,353
	10 20 30 40 50 60 MERLQRIFGA GGGLGHASPD SPTLDTSEQV YISSLALLKM LKHGRAGVPM EVMGLMLGEF 70 80 90 100 110 120 VDEYTVRVVD VFAMPQSGTG VSVEAVDHVF QTNMLDMLKQ TGRPEMVVGW YHSHPGFGCW 130 140 150 160 170 180 LSGVDINTQQ SFEALNQRAV AVVVDPIQSV KGKVVIDAFR SINPQTIMLG QEPRQTTSNL 190 200 210 220 230 240 GHLNKPSIQA LIHGLNRHYY SIAINYRKNE LEEKMLLNLH KKKWTDGLTL RRFDTHSKTN 250 260 270 280 290 300 EQTVQEMLSL AAKYNKAVQE EDELSPEKLA IVNVGRQDAK KHLEEHVSNL MSSNIVQTLG TMLDTVVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms." Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D. J. Biol. Chem. 279:6401-6413(2004) [PubMed: 14623884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY230843 mRNA. Translation: AAP86670.1. AY230844 mRNA. Translation: AAP86671.1. AY230845 mRNA. Translation: AAP86672.1. AB025633 Genomic DNA. Translation: BAA97246.1. AY070073 mRNA. Translation: AAL49768.1. AY091329 mRNA. Translation: AAM14268.1. AY086277 mRNA. Translation: AAM64349.1.
IPI	IPI00538427.
RefSeq	NP_197745.1.
UniGene	At.22926
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LT08.
Protein family/group databases
MEROPS	M67.001.
Proteomic databases
PRIDE	Q9LT08.
Genome annotation databases
GeneID	832420.
GenomeReviews	Gene locus AT5G23540 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.24532.
Organism-specific databases
TAIR	At5g23540.
Phylogenomic databases
OMA	QDPKKHL.
Gene expression databases
ArrayExpress	Q9LT08.
Genevestigator	Q9LT08.
GermOnline	AT5G23540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
ProDom	PD363422. Mov34-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSDE_ARATH

Accession

Primary (citable) accession number: Q9LT08
Secondary accession number(s): Q8LD11

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)