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Protein

Probable manganese-transporting ATPase PDR2

Gene

PDR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis (By similarity). Plays an important role in pollen and root development through its impact on protein secretion and transport processes. Functions together with LPR1 and LPR2 in a common pathway that adjusts root meristem activity to phosphate availability. Under phosphate limitation, restricts SHR movement in root meristem and is required for maintaining SCR expression in the root meristem stem-cell niche as well as for proximal meristem activity. Can complement the yeast spf1 mutant.By similarity3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei491 – 49114-aspartylphosphate intermediateBy similarity
Metal bindingi812 – 8121MagnesiumBy similarity
Metal bindingi816 – 8161MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • cation transport Source: InterPro
  • cellular calcium ion homeostasis Source: GO_Central
  • cellular metal ion homeostasis Source: TAIR
  • cellular response to phosphate starvation Source: TAIR
  • meristem maintenance Source: TAIR
  • pollen germination Source: TAIR
  • pollen maturation Source: TAIR
  • stem cell fate determination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable manganese-transporting ATPase PDR2 (EC:3.6.3.-)
Alternative name(s):
Protein MALE GAMETOGENESIS IMPAIRED ANTHERS
Protein PHOSPHATE DEFICIENCY RESPONSE 2
Gene namesi
Name:PDR2
Synonyms:MIA
Ordered Locus Names:At5g23630
ORF Names:MQM1.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23630.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence analysisAdd
BLAST
Transmembranei21 – 4222HelicalSequence analysisAdd
BLAST
Topological domaini43 – 508LumenalSequence analysis
Transmembranei51 – 7121HelicalSequence analysisAdd
BLAST
Topological domaini72 – 192121CytoplasmicSequence analysisAdd
BLAST
Transmembranei193 – 21523HelicalSequence analysisAdd
BLAST
Topological domaini216 – 2183LumenalSequence analysis
Transmembranei219 – 23820HelicalSequence analysisAdd
BLAST
Topological domaini239 – 402164CytoplasmicSequence analysisAdd
BLAST
Transmembranei403 – 42220HelicalSequence analysisAdd
BLAST
Topological domaini423 – 43513LumenalSequence analysisAdd
BLAST
Transmembranei436 – 45318HelicalSequence analysisAdd
BLAST
Topological domaini454 – 947494CytoplasmicSequence analysisAdd
BLAST
Transmembranei948 – 96720HelicalSequence analysisAdd
BLAST
Topological domaini968 – 97912LumenalSequence analysisAdd
BLAST
Transmembranei980 – 99718HelicalSequence analysisAdd
BLAST
Topological domaini998 – 101316CytoplasmicSequence analysisAdd
BLAST
Transmembranei1014 – 103421HelicalSequence analysisAdd
BLAST
Topological domaini1035 – 105925LumenalSequence analysisAdd
BLAST
Transmembranei1060 – 107920HelicalSequence analysisAdd
BLAST
Topological domaini1080 – 109213CytoplasmicSequence analysisAdd
BLAST
Transmembranei1093 – 111018HelicalSequence analysisAdd
BLAST
Topological domaini1111 – 112818LumenalSequence analysisAdd
BLAST
Transmembranei1129 – 114820HelicalSequence analysisAdd
BLAST
Topological domaini1149 – 117931CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Imbalances in cation homeostasis and severe reduction in fertility. Increased inhibition of primary root growth in low inorganic phosphate conditions.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi699 – 6991T → I in pdr2-1; increased inhibition of primary root growth in low inorganic phosphate conditions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11791179Probable manganese-transporting ATPase PDR2PRO_0000046420Add
BLAST

Proteomic databases

PaxDbiQ9LT02.
PRIDEiQ9LT02.

Expressioni

Tissue specificityi

Highly expressed in root meristem. Expressed in pavement cells of trichomes, stipules, stamens and pollen grains.1 Publication

Gene expression databases

GenevisibleiQ9LT02. AT.

Interactioni

Protein-protein interaction databases

BioGridi17703. 1 interaction.
STRINGi3702.AT5G23630.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LT02.
SMRiQ9LT02. Positions 255-843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1175 – 11784Poly-Lys

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0209. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000199432.
InParanoidiQ9LT02.
KOiK14950.
OMAiYLYSEAQ.
PhylomeDBiQ9LT02.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF82. PTHR24093:SF82. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LT02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFRVGGKV VEKVDLCRKK QLVWRLDVWP FAILYTVWLT TIVPSIDFSD
60 70 80 90 100
ACIALGGLSA FHILVLLFTT WSVDFKCFVQ FSKVNSIDQA DACKVTPAKF
110 120 130 140 150
SGSKEVVPLH FRSQMTDSAS SGDMEEIFFD FRKQRFIYSK ELGAFSKLPY
160 170 180 190 200
PTKETFGHYL KCTGHGTEAK IATATEKWGR NVFDYPQPTF QKLMKENCME
210 220 230 240 250
PFFVFQVFCV GLWCLDEFWY YSVFTLFMLF MFESTMAKSR LKTLTDLRSV
260 270 280 290 300
RVDSQTVMVY RSGKWVKLLG TDLLPGDVVS IGRPSTQTGG EDKTVPADML
310 320 330 340 350
LLVGSAIVNE AILTGESTPQ WKVPIVGQRS DEKLSIKRNK NHVLFGGTKI
360 370 380 390 400
LQHSPDKSFS LKTPDGGCLA VVLRTGFETS QGKLMRTILF STERVTANSW
410 420 430 440 450
ESGLFILFLV VFAVIAAGYV LVKGLEDPTR SKYKLLLGCS LIITSVIPPE
460 470 480 490 500
LPMELSIAVN TSLLALVRRG IFCTEPFRIP FAGKVDLCCF DKTGTLTSDD
510 520 530 540 550
MEFRGVGGLS NCEEAETDMS KVPVRTLEIL ASCHALVFVE NKLVGDPLEK
560 570 580 590 600
AALKGIDWSY KADEKALPRR GNGNSVQIMQ RYHFASHLKR MSVIVRIQEE
610 620 630 640 650
YLAFVKGAPE TIQERLVDVP AQYIETYKRY TRQGSRVLAL AYKRLPDMMV
660 670 680 690 700
SEARDMDRDA VESDLTFAGF AVFNCPIRPD SAPVLLELKN SSHDLVMITG
710 720 730 740 750
DQALTACHVA GQVHIVSNPV LILGRSGSGN EYKWVSPDEK EIIPYSEKEI
760 770 780 790 800
ETLAETHDLC IGGDSIEMLQ ATSAVLRVIP FVKVFARVAP QQKELILTTF
810 820 830 840 850
KAVGRGTLMC GDGTNDVGAL KQAHVGVALL NNKLPLSPSD SSKDDKSKSK
860 870 880 890 900
KSKLPLEPAS KTITQNGEGS SKGKIPPQNR HLTAAELQRQ KLKKIMDDLN
910 920 930 940 950
NDEGDGRSAP LVKLGDASMA SPFTAKHASV APVTDIIRQG RSTLVTTLQM
960 970 980 990 1000
FKILGLNCLA TAYVLSVMYL DGVKLGDVQA TISGVLTAAF FLFISHARPL
1010 1020 1030 1040 1050
QTLSAERPHP SVFSVYLFLS LIGQFAVHLT FLVYSVKEAE KHMPEECIEP
1060 1070 1080 1090 1100
DASFHPNLVN TVSYMVSMML QVATFAVNYM GHPFNQSIRE NKPFFYALIA
1110 1120 1130 1140 1150
GAGFFTVIAS DLFRDLNDSL KLVPLPQGLR DKLLIWASLM FIICYSWERL
1160 1170
LRWAFPGKIS SWKHKQRAVT ANLEKKKKV
Length:1,179
Mass (Da):131,115
Last modified:October 1, 2000 - v1
Checksum:i4A3E82D2222A4D78
GO

Sequence cautioni

The sequence BX831910 differs from that shown. Reason: Frameshift at position 1100. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti935 – 9351D → N in BX831910 (PubMed:14993207).Curated
Sequence conflicti995 – 9951S → F in BX831910 (PubMed:14993207).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025633 Genomic DNA. Translation: BAA97238.1.
CP002688 Genomic DNA. Translation: AED93192.1.
BX831910 mRNA. No translation available.
RefSeqiNP_197752.1. NM_122268.4.
UniGeneiAt.25308.

Genome annotation databases

EnsemblPlantsiAT5G23630.1; AT5G23630.1; AT5G23630.
GeneIDi832428.
GrameneiAT5G23630.1; AT5G23630.1; AT5G23630.
KEGGiath:AT5G23630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025633 Genomic DNA. Translation: BAA97238.1.
CP002688 Genomic DNA. Translation: AED93192.1.
BX831910 mRNA. No translation available.
RefSeqiNP_197752.1. NM_122268.4.
UniGeneiAt.25308.

3D structure databases

ProteinModelPortaliQ9LT02.
SMRiQ9LT02. Positions 255-843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17703. 1 interaction.
STRINGi3702.AT5G23630.1.

Proteomic databases

PaxDbiQ9LT02.
PRIDEiQ9LT02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23630.1; AT5G23630.1; AT5G23630.
GeneIDi832428.
GrameneiAT5G23630.1; AT5G23630.1; AT5G23630.
KEGGiath:AT5G23630.

Organism-specific databases

TAIRiAT5G23630.

Phylogenomic databases

eggNOGiKOG0209. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000199432.
InParanoidiQ9LT02.
KOiK14950.
OMAiYLYSEAQ.
PhylomeDBiQ9LT02.

Enzyme and pathway databases

ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9LT02.

Gene expression databases

GenevisibleiQ9LT02. AT.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006544. P-type_TPase_V.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF82. PTHR24093:SF82. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 2 hits.
TIGR01657. P-ATPase-V. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 702-1179.
    Strain: cv. Columbia.
  4. "Arabidopsis pdr2 reveals a phosphate-sensitive checkpoint in root development."
    Ticconi C.A., Delatorre C.A., Lahner B., Salt D.E., Abel S.
    Plant J. 37:801-814(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a type V P-type ATPase."
    Jakobsen M.K., Poulsen L.R., Schulz A., Fleurat-Lessard P., Moller A., Husted S., Schiott M., Amtmann A., Palmgren M.G.
    Genes Dev. 19:2757-2769(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. "ER-resident proteins PDR2 and LPR1 mediate the developmental response of root meristems to phosphate availability."
    Ticconi C.A., Lucero R.D., Sakhonwasee S., Adamson A.W., Creff A., Nussaume L., Desnos T., Abel S.
    Proc. Natl. Acad. Sci. U.S.A. 106:14174-14179(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF THR-699.

Entry informationi

Entry nameiPDR2_ARATH
AccessioniPrimary (citable) accession number: Q9LT02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.