Q9LSY9 (U71B1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 71B1
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71B1
EC=2.4.1.91

Gene names

Name:	UGT71B1
Ordered Locus Names:	At3g21750
ORF Names:	MSD21.6, MSD21.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase activity in vitro. Also active in vitro on benzoates and benzoate derivatives. Ref.6 Ref.7
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Sequence caution	The sequence AAK32764.1 differs from that shown. Reason: Frameshift at position 455.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	UDP-glycosyltransferase 71B1		PRO_0000409047
Regions
	Region	342 – 344	3	UDP-glucose binding By similarity
	Region	359 – 367	9	UDP-glucose binding By similarity
	Region	381 – 384	4	UDP-glucose binding By similarity
Sites
	Binding site	273	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LSY9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 42B7925984C350B0	FASTA	473	52,666
	10 20 30 40 50 60 MKVELVFIPS PGVGHIRATT ALAKLLVASD NRLSVTLIVI PSRVSDDASS SVYTNSEDRL 70 80 90 100 110 120 RYILLPARDQ TTDLVSYIDS QKPQVRAVVS KVAGDVSTRS DSRLAGIVVD MFCTSMIDIA 130 140 150 160 170 180 DEFNLSAYIF YTSNASYLGL QFHVQSLYDE KELDVSEFKD TEMKFDVPTL TQPFPAKCLP 190 200 210 220 230 240 SVMLNKKWFP YVLGRARSFR ATKGILVNSV ADMEPQALSF FSGGNGNTNI PPVYAVGPIM 250 260 270 280 290 300 DLESSGDEEK RKEILHWLKE QPTKSVVFLC FGSMGGFSEE QAREIAVALE RSGHRFLWSL 310 320 330 340 350 360 RRASPVGNKS NPPPGEFTNL EEILPKGFLD RTVEIGKIIS WAPQVDVLNS PAIGAFVTHC 370 380 390 400 410 420 GWNSILESLW FGVPMAAWPI YAEQQFNAFH MVDELGLAAE VKKEYRRDFL VEEPEIVTAD 430 440 450 460 470 EIERGIKCAM EQDSKMRKRV MEMKDKLHVA LVDGGSSNCA LKKFVQDVVD NVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates." Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J. J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025634 Genomic DNA. Translation: BAB02837.1. CP002686 Genomic DNA. Translation: AEE76548.1. AF361596 mRNA. Translation: AAK32764.1. Frameshift. AK227147 mRNA. Translation: BAE99192.1.
IPI	IPI00536194.
RefSeq	NP_188812.1. NM_113070.2.
UniGene	At.19110. At.66536.
3D structure databases
ProteinModelPortal	Q9LSY9.
SMR	Q9LSY9. Positions 4-468.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G21750.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q9LSY9.
PRIDE	Q9LSY9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21750.1; AT3G21750.1; AT3G21750.
GeneID	821729.
KEGG	ath:AT3G21750.
Organism-specific databases
TAIR	At3g21750.
Phylogenomic databases
eggNOG	KOG1192.
HOGENOM	HOG000237568.
InParanoid	Q9LSY9.
OMA	GHIRATT.
PhylomeDB	Q9LSY9.
ProtClustDB	PLN02554.
Enzyme and pathway databases
BioCyc	ARA:AT3G21750-MONOMER. MetaCyc:AT3G21750-MONOMER.
Gene expression databases
Genevestigator	Q9LSY9.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U71B1_ARATH

Accession

Primary (citable) accession number: Q9LSY9
Secondary accession number(s): Q9ASY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program