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Protein

Electron transfer flavoprotein subunit beta, mitochondrial

Gene

ETFB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity). Involved in leucine catabolism and in phytol degradation.By similarity1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per dimer.By similarity
  • AMPBy similarityNote: Binds 1 AMP per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

  • chlorophyll catabolic process Source: TAIR
  • leucine catabolic process Source: TAIR
  • oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G43430-MONOMER.
ReactomeiR-ATH-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit beta, mitochondrial
Short name:
Beta-ETF
Gene namesi
Name:ETFB
Ordered Locus Names:At5g43430
ORF Names:MWF20.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G43430.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Reduced plant viability on extended exposure to darkness and a reduced reproductive performance under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 251Electron transfer flavoprotein subunit beta, mitochondrialPRO_0000324180
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ9LSW8.
PRIDEiQ9LSW8.

PTM databases

iPTMnetiQ9LSW8.

Expressioni

Inductioni

Expressed constitutively. Not induced by dark treatment or sucrose starvation.1 Publication

Gene expression databases

ExpressionAtlasiQ9LSW8. baseline and differential.
GenevisibleiQ9LSW8. AT.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Protein-protein interaction databases

IntActiQ9LSW8. 1 interaction.
STRINGi3702.AT5G43430.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LSW8.
SMRiQ9LSW8. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETF beta-subunit/FixA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3180. Eukaryota.
COG2086. LUCA.
HOGENOMiHOG000247877.
InParanoidiQ9LSW8.
OMAiNPFCDIA.
PhylomeDBiQ9LSW8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LSW8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILVAVKRV VDYAVKIRVK PDKTGVETQN VKMSMNPFCE IALEEALRIK
60 70 80 90 100
EAGFAKEVIA VSIGPSQCVD TLRTGLAMGA DRGIHVETNS IFLPLTIAKI
110 120 130 140 150
LKSLADVENP GLIFLGKQAI DDDCNQTGQM VAALLGWPQA TFASKVVLDK
160 170 180 190 200
DKNVATVDRE VDGGLETLNV DLPAVITTDL RLNQPRYASL PNIMKAKSKP
210 220 230 240 250
IKKMTVQDLK VDIKSDIEIL EVTEPPKRKS GVMVSSVDEL IDKLKNEAHV

V
Length:251
Mass (Da):27,400
Last modified:October 1, 2000 - v1
Checksum:i5C5B8146CA02AF7F
GO
Isoform 2 (identifier: Q9LSW8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: T → S
     179-251: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:178
Mass (Da):19,134
Checksum:i0FA666D9F8565449
GO
Isoform 3 (identifier: Q9LSW8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:219
Mass (Da):23,792
Checksum:i3CC7A09A21B4C790
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911I → S in AAM61464 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3232Missing in isoform 3. CuratedVSP_032173Add
BLAST
Alternative sequencei178 – 1781T → S in isoform 2. CuratedVSP_032174
Alternative sequencei179 – 25173Missing in isoform 2. CuratedVSP_032175Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025638 Genomic DNA. Translation: BAA97422.1.
CP002688 Genomic DNA. Translation: AED94960.1.
CP002688 Genomic DNA. Translation: AED94961.1.
CP002688 Genomic DNA. Translation: AED94962.1.
AY045657 mRNA. Translation: AAK74015.1.
AF446867 mRNA. Translation: AAL38600.1.
AY084901 mRNA. Translation: AAM61464.1.
RefSeqiNP_001032002.1. NM_001036925.2. [Q9LSW8-2]
NP_001078699.1. NM_001085230.2. [Q9LSW8-3]
NP_199156.1. NM_123709.3. [Q9LSW8-1]
UniGeneiAt.66670.
At.7156.

Genome annotation databases

EnsemblPlantsiAT5G43430.1; AT5G43430.1; AT5G43430. [Q9LSW8-1]
GeneIDi834363.
KEGGiath:AT5G43430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025638 Genomic DNA. Translation: BAA97422.1.
CP002688 Genomic DNA. Translation: AED94960.1.
CP002688 Genomic DNA. Translation: AED94961.1.
CP002688 Genomic DNA. Translation: AED94962.1.
AY045657 mRNA. Translation: AAK74015.1.
AF446867 mRNA. Translation: AAL38600.1.
AY084901 mRNA. Translation: AAM61464.1.
RefSeqiNP_001032002.1. NM_001036925.2. [Q9LSW8-2]
NP_001078699.1. NM_001085230.2. [Q9LSW8-3]
NP_199156.1. NM_123709.3. [Q9LSW8-1]
UniGeneiAt.66670.
At.7156.

3D structure databases

ProteinModelPortaliQ9LSW8.
SMRiQ9LSW8. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9LSW8. 1 interaction.
STRINGi3702.AT5G43430.1.

PTM databases

iPTMnetiQ9LSW8.

Proteomic databases

PaxDbiQ9LSW8.
PRIDEiQ9LSW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G43430.1; AT5G43430.1; AT5G43430. [Q9LSW8-1]
GeneIDi834363.
KEGGiath:AT5G43430.

Organism-specific databases

TAIRiAT5G43430.

Phylogenomic databases

eggNOGiKOG3180. Eukaryota.
COG2086. LUCA.
HOGENOMiHOG000247877.
InParanoidiQ9LSW8.
OMAiNPFCDIA.
PhylomeDBiQ9LSW8.

Enzyme and pathway databases

BioCyciARA:AT5G43430-MONOMER.
ReactomeiR-ATH-611105. Respiratory electron transport.

Miscellaneous databases

PROiQ9LSW8.

Gene expression databases

ExpressionAtlasiQ9LSW8. baseline and differential.
GenevisibleiQ9LSW8. AT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR000049. ET-Flavoprotein_bsu_CS.
IPR014730. ETF_a/b_N.
IPR012255. ETF_b.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR21294. PTHR21294. 1 hit.
PfamiPF01012. ETF. 1 hit.
[Graphical view]
PIRSFiPIRSF000090. Beta-ETF. 1 hit.
SMARTiSM00893. ETF. 1 hit.
[Graphical view]
PROSITEiPS01065. ETF_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  6. "The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness."
    Ishizaki K., Schauer N., Larson T.R., Graham I.A., Fernie A.R., Leaver C.J.
    Plant J. 47:751-760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, INTERACTION WITH ETFA, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiETFB_ARATH
AccessioniPrimary (citable) accession number: Q9LSW8
Secondary accession number(s): A8MRD3, Q2V317, Q8LFE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.