Reviewed, UniProtKB/Swiss-Prot Q9LSU1 (PSA5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-5
    EC=3.4.25.1
Alternative name(s):
    20S proteasome alpha subunit E
    20S proteasome subunit alpha-5

Gene names

Name:	PAE1
Ordered Locus Names:	Os11g0615700, LOC_Os11g40140

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.
Sequence caution	The sequence ABA94697.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Proteasome subunit alpha type-5		PRO_0000124126

Sequences

	Sequence		Length	Mass (Da)
	Q9LSU1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E4EB5319CC409FA1	FASTA	237	25,993
	10 20 30 40 50 60 MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGL KTKDGVVLAV EKRVTSPLLE 70 80 90 100 110 120 PSSVEKIMEI DEHIGCAMSG LIADARTLVE HARVETQNHR FSYGEPMTVE STTQAICDLA 130 140 150 160 170 180 LRFGEGDEES MSRPFGVSLL IAGHDENGPS LYYTDPSGTF WQCNAKAIGS GSEGADSSLQ 190 200 210 220 230 EQYNKELTLQ EAETIALSIL KQVMEEKVTP NNVDIAKVSP NYHLYTPAEV EAVIARL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)." Sassa H., Oguchi S., Inoue T., Hirano H. Gene 250:61-66(2000) [PubMed: 10854779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026561 mRNA. Translation: BAA96832.1. DP000010 Genomic DNA. Translation: ABA94696.1. DP000010 Genomic DNA. Translation: ABA94697.1. Sequence problems.
RefSeq	NP_001068278.1.
UniGene	Os.3705
3D structure databases
SMR	Q9LSU1. Positions 8-234.
ModBase	Search...
Protein family/group databases
MEROPS	T01.975.
Genome annotation databases
GeneID	4350922.
KEGG	osa:4350922.
Organism-specific databases
Gramene	Q9LSU1.
Phylogenomic databases
PhylomeDB	Q9LSU1.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA5_ORYSJ

Accession

Primary (citable) accession number: Q9LSU1
Secondary accession number(s): Q2R171, Q2R172

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)