Q9LSU0 (PSA3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-3
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit G
20S proteasome subunit alpha-7

Gene names

Name:	PAG1
Ordered Locus Names:	Os01g0811100, LOC_Os01g59600
ORF Names:	P0425G02.8, P0468B07.45

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Proteasome subunit alpha type-3		PRO_0000124099

Sequences

	Sequence		Length	Mass (Da)
	Q9LSU0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E6D37062AC03E31E	FASTA	249	27,238
	10 20 30 40 50 60 MSSIGTGYDL SVTTFSPDGR VFQVEYATKA VDNSGTVVGI KCKDGIVLGV EKLVTSKMML 70 80 90 100 110 120 EGSNRRIHSV HWHSGLAVAG LAADGRQIVS RAKSEAASYE KVYGEPISVK ELADRVASYV 130 140 150 160 170 180 HLCTLYWWLR PFGCGVILGG YDRDGPQLYM IEPSGVSYKY FGAALGKGRQ AAKTEIEKLK 190 200 210 220 230 240 LSELTCREGI VEVAKIIYGV HDEAKDKAFE LELSWICDES NRQHQKVPAD LLEQAKVAAQ AALEEMDAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)." Sassa H., Oguchi S., Inoue T., Hirano H. Gene 250:61-66(2000) [PubMed: 10854779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026562 mRNA. Translation: BAA96833.1. AP003247 Genomic DNA. Translation: BAD68202.1. AP003260 Genomic DNA. Translation: BAD68244.1.
RefSeq	NP_001044588.1. NM_001051123.1.
UniGene	Os.3706.
3D structure databases
ProteinModelPortal	Q9LSU0.
SMR	Q9LSU0. Positions 2-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LSU0.
Protein family/group databases
MEROPS	T01.977.
Proteomic databases
PRIDE	Q9LSU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g59600.1; LOC_Os01g59600.1; LOC_Os01g59600. LOC_Os01g59600.2; LOC_Os01g59600.2; LOC_Os01g59600.
GeneID	4327357.
KEGG	osa:4327357.
NMPDR	fig|39947.1.peg.7997.
Organism-specific databases
Gramene	Q9LSU0.
Phylogenomic databases
GeneTree	EPGT00050000005272.
OMA	DIPLKHL.
PhylomeDB	Q9LSU0.
ProtClustDB	CLSN2683434.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02727.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA3_ORYSJ

Accession

Primary (citable) accession number: Q9LSU0
Secondary accession number(s): Q5VQP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program