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Q9LSS3 (SEN22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit Sen2-2

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease Sen2-2
Short name=AtSen2
Gene names
Name:SEN2
Ordered Locus Names:At5g60230
ORF Names:F15L12.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
   Biological processmRNA processing
tRNA processing
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LSS3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA-splicing endonuclease subunit Sen2-2
PRO_0000109457

Sites

Active site1481 By similarity
Active site1561 By similarity
Active site1891 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: DFAEC30162214014

FASTA25028,519
        10         20         30         40         50         60 
MAPRWKWKGA EAKALAEPIS KSVSELQLSL AETESSGTLS SCNVLLAVEP EQAELLDRCC 

        70         80         90        100        110        120 
FGRLVLSAEK IKKWIQLSFE EAFFLHYNLK CIKISLQGRC LENEVDTWLY MKSKRPNFPM 

       130        140        150        160        170        180 
FFKAYSHLRS KNWVLRSGLQ YGVDFVAYRH HPSLVHSEYS VLVQSGDSDR LRVWSDIHCA 

       190        200        210        220        230        240 
VRLSGSVAKT LLTLYVNGNF KGEDVNLLVC LENFTVEEQT ISRWSPELSR EDQSTNSKQH 

       250 
VPNVSNLNTL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
Akama K., Junker V., Beier H.
Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia and cv. Landsberg erecta.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026632 Genomic DNA. Translation: BAA97504.1.
CP002688 Genomic DNA. Translation: AED97296.1.
BT004112 mRNA. Translation: AAO42135.1.
BT004920 mRNA. Translation: AAO50453.1.
RefSeqNP_200831.1. NM_125416.3.
UniGeneAt.29175.

3D structure databases

ProteinModelPortalQ9LSS3.
SMRQ9LSS3. Positions 35-226.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9LSS3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G60230.1; AT5G60230.1; AT5G60230. [Q9LSS3-1]
GeneID836145.
KEGGath:AT5G60230.

Organism-specific databases

GeneFarm5165. 493.
TAIRAT5G60230.

Phylogenomic databases

eggNOGNOG305469.
HOGENOMHOG000030044.
InParanoidQ9LSS3.
KOK15322.
PhylomeDBQ9LSS3.
ProtClustDBCLSN2684758.

Enzyme and pathway databases

BioCycARA:AT5G60230-MONOMER.
ARA:GQT-2328-MONOMER.

Gene expression databases

GenevestigatorQ9LSS3.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Other

PROQ9LSS3.

Entry information

Entry nameSEN22_ARATH
AccessionPrimary (citable) accession number: Q9LSS3
Secondary accession number(s): Q3E887
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names