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Protein

tRNA-splicing endonuclease subunit Sen2-2

Gene

SEN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148By similarity1
Active sitei156By similarity1
Active sitei189By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2-2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2-2
Short name:
AtSen2
Gene namesi
Name:SEN2
Ordered Locus Names:At5g60230
ORF Names:F15L12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G60230

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094571 – 250tRNA-splicing endonuclease subunit Sen2-2Add BLAST250

Proteomic databases

PaxDbiQ9LSS3
PRIDEiQ9LSS3

Expressioni

Gene expression databases

ExpressionAtlasiQ9LSS3 baseline and differential
GenevisibleiQ9LSS3 AT

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G60230.2

Structurei

3D structure databases

ProteinModelPortaliQ9LSS3
SMRiQ9LSS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4685 Eukaryota
COG1676 LUCA
HOGENOMiHOG000030044
InParanoidiQ9LSS3
KOiK15322
PhylomeDBiQ9LSS3

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR006676 tRNA_splic
PANTHERiPTHR21227 PTHR21227, 2 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LSS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRWKWKGA EAKALAEPIS KSVSELQLSL AETESSGTLS SCNVLLAVEP
60 70 80 90 100
EQAELLDRCC FGRLVLSAEK IKKWIQLSFE EAFFLHYNLK CIKISLQGRC
110 120 130 140 150
LENEVDTWLY MKSKRPNFPM FFKAYSHLRS KNWVLRSGLQ YGVDFVAYRH
160 170 180 190 200
HPSLVHSEYS VLVQSGDSDR LRVWSDIHCA VRLSGSVAKT LLTLYVNGNF
210 220 230 240 250
KGEDVNLLVC LENFTVEEQT ISRWSPELSR EDQSTNSKQH VPNVSNLNTL
Length:250
Mass (Da):28,519
Last modified:October 1, 2000 - v1
Checksum:iDFAEC30162214014
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026632 Genomic DNA Translation: BAA97504.1
CP002688 Genomic DNA Translation: AED97296.1
BT004112 mRNA Translation: AAO42135.1
BT004920 mRNA Translation: AAO50453.1
RefSeqiNP_200831.1, NM_125416.4 [Q9LSS3-1]
UniGeneiAt.29175

Genome annotation databases

EnsemblPlantsiAT5G60230.1; AT5G60230.1; AT5G60230 [Q9LSS3-1]
AT5G60230.2; AT5G60230.2; AT5G60230
GeneIDi836145
GrameneiAT5G60230.1; AT5G60230.1; AT5G60230 [Q9LSS3-1]
AT5G60230.2; AT5G60230.2; AT5G60230
KEGGiath:AT5G60230

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSEN22_ARATH
AccessioniPrimary (citable) accession number: Q9LSS3
Secondary accession number(s): Q3E887
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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