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Q9LSS3

- SEN22_ARATH

UniProt

Q9LSS3 - SEN22_ARATH

Protein

tRNA-splicing endonuclease subunit Sen2-2

Gene

SEN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei148 – 1481By similarity
    Active sitei156 – 1561By similarity
    Active sitei189 – 1891By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Enzyme and pathway databases

    BioCyciARA:AT5G60230-MONOMER.
    ARA:GQT-2328-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen2-2 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease Sen2-2
    Short name:
    AtSen2
    Gene namesi
    Name:SEN2
    Ordered Locus Names:At5g60230
    ORF Names:F15L12.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G60230.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250tRNA-splicing endonuclease subunit Sen2-2PRO_0000109457Add
    BLAST

    Proteomic databases

    PRIDEiQ9LSS3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LSS3.

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LSS3.
    SMRiQ9LSS3. Positions 67-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiNOG305469.
    HOGENOMiHOG000030044.
    InParanoidiQ9LSS3.
    KOiK15322.
    PhylomeDBiQ9LSS3.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9LSS3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MAPRWKWKGA EAKALAEPIS KSVSELQLSL AETESSGTLS SCNVLLAVEP    50
    EQAELLDRCC FGRLVLSAEK IKKWIQLSFE EAFFLHYNLK CIKISLQGRC 100
    LENEVDTWLY MKSKRPNFPM FFKAYSHLRS KNWVLRSGLQ YGVDFVAYRH 150
    HPSLVHSEYS VLVQSGDSDR LRVWSDIHCA VRLSGSVAKT LLTLYVNGNF 200
    KGEDVNLLVC LENFTVEEQT ISRWSPELSR EDQSTNSKQH VPNVSNLNTL 250
    Length:250
    Mass (Da):28,519
    Last modified:October 1, 2000 - v1
    Checksum:iDFAEC30162214014
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026632 Genomic DNA. Translation: BAA97504.1.
    CP002688 Genomic DNA. Translation: AED97296.1.
    BT004112 mRNA. Translation: AAO42135.1.
    BT004920 mRNA. Translation: AAO50453.1.
    RefSeqiNP_200831.1. NM_125416.3. [Q9LSS3-1]
    UniGeneiAt.29175.

    Genome annotation databases

    EnsemblPlantsiAT5G60230.1; AT5G60230.1; AT5G60230. [Q9LSS3-1]
    AT5G60230.2; AT5G60230.2; AT5G60230.
    GeneIDi836145.
    KEGGiath:AT5G60230.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026632 Genomic DNA. Translation: BAA97504.1 .
    CP002688 Genomic DNA. Translation: AED97296.1 .
    BT004112 mRNA. Translation: AAO42135.1 .
    BT004920 mRNA. Translation: AAO50453.1 .
    RefSeqi NP_200831.1. NM_125416.3. [Q9LSS3-1 ]
    UniGenei At.29175.

    3D structure databases

    ProteinModelPortali Q9LSS3.
    SMRi Q9LSS3. Positions 67-209.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9LSS3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G60230.1 ; AT5G60230.1 ; AT5G60230 . [Q9LSS3-1 ]
    AT5G60230.2 ; AT5G60230.2 ; AT5G60230 .
    GeneIDi 836145.
    KEGGi ath:AT5G60230.

    Organism-specific databases

    GeneFarmi 5165. 493.
    TAIRi AT5G60230.

    Phylogenomic databases

    eggNOGi NOG305469.
    HOGENOMi HOG000030044.
    InParanoidi Q9LSS3.
    KOi K15322.
    PhylomeDBi Q9LSS3.

    Enzyme and pathway databases

    BioCyci ARA:AT5G60230-MONOMER.
    ARA:GQT-2328-MONOMER.

    Miscellaneous databases

    PROi Q9LSS3.

    Gene expression databases

    Genevestigatori Q9LSS3.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
      Akama K., Junker V., Beier H.
      Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia and cv. Landsberg erecta.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
      Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiSEN22_ARATH
    AccessioniPrimary (citable) accession number: Q9LSS3
    Secondary accession number(s): Q3E887
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3