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Q9LSS3

- SEN22_ARATH

UniProt

Q9LSS3 - SEN22_ARATH

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Protein
tRNA-splicing endonuclease subunit Sen2-2
Gene
SEN2, At5g60230, F15L12.18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei148 – 1481 By similarity
Active sitei156 – 1561 By similarity
Active sitei189 – 1891 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

mRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciARA:AT5G60230-MONOMER.
ARA:GQT-2328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2-2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2-2
Short name:
AtSen2
Gene namesi
Name:SEN2
Ordered Locus Names:At5g60230
ORF Names:F15L12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G60230.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250tRNA-splicing endonuclease subunit Sen2-2
PRO_0000109457Add
BLAST

Proteomic databases

PRIDEiQ9LSS3.

Expressioni

Gene expression databases

GenevestigatoriQ9LSS3.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9LSS3.
SMRiQ9LSS3. Positions 67-209.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG305469.
HOGENOMiHOG000030044.
InParanoidiQ9LSS3.
KOiK15322.
PhylomeDBiQ9LSS3.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LSS3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAPRWKWKGA EAKALAEPIS KSVSELQLSL AETESSGTLS SCNVLLAVEP    50
EQAELLDRCC FGRLVLSAEK IKKWIQLSFE EAFFLHYNLK CIKISLQGRC 100
LENEVDTWLY MKSKRPNFPM FFKAYSHLRS KNWVLRSGLQ YGVDFVAYRH 150
HPSLVHSEYS VLVQSGDSDR LRVWSDIHCA VRLSGSVAKT LLTLYVNGNF 200
KGEDVNLLVC LENFTVEEQT ISRWSPELSR EDQSTNSKQH VPNVSNLNTL 250
Length:250
Mass (Da):28,519
Last modified:October 1, 2000 - v1
Checksum:iDFAEC30162214014
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026632 Genomic DNA. Translation: BAA97504.1.
CP002688 Genomic DNA. Translation: AED97296.1.
BT004112 mRNA. Translation: AAO42135.1.
BT004920 mRNA. Translation: AAO50453.1.
RefSeqiNP_200831.1. NM_125416.3. [Q9LSS3-1]
UniGeneiAt.29175.

Genome annotation databases

EnsemblPlantsiAT5G60230.1; AT5G60230.1; AT5G60230. [Q9LSS3-1]
AT5G60230.2; AT5G60230.2; AT5G60230.
GeneIDi836145.
KEGGiath:AT5G60230.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026632 Genomic DNA. Translation: BAA97504.1 .
CP002688 Genomic DNA. Translation: AED97296.1 .
BT004112 mRNA. Translation: AAO42135.1 .
BT004920 mRNA. Translation: AAO50453.1 .
RefSeqi NP_200831.1. NM_125416.3. [Q9LSS3-1 ]
UniGenei At.29175.

3D structure databases

ProteinModelPortali Q9LSS3.
SMRi Q9LSS3. Positions 67-209.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9LSS3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G60230.1 ; AT5G60230.1 ; AT5G60230 . [Q9LSS3-1 ]
AT5G60230.2 ; AT5G60230.2 ; AT5G60230 .
GeneIDi 836145.
KEGGi ath:AT5G60230.

Organism-specific databases

GeneFarmi 5165. 493.
TAIRi AT5G60230.

Phylogenomic databases

eggNOGi NOG305469.
HOGENOMi HOG000030044.
InParanoidi Q9LSS3.
KOi K15322.
PhylomeDBi Q9LSS3.

Enzyme and pathway databases

BioCyci ARA:AT5G60230-MONOMER.
ARA:GQT-2328-MONOMER.

Miscellaneous databases

PROi Q9LSS3.

Gene expression databases

Genevestigatori Q9LSS3.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana."
    Akama K., Junker V., Beier H.
    Gene 257:177-185(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiSEN22_ARATH
AccessioniPrimary (citable) accession number: Q9LSS3
Secondary accession number(s): Q3E887
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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