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Q9LSP7 (UBC32_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 32

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 32
Gene names
Name:UBC32
Ordered Locus Names:At3g17000
ORF Names:K14A17.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Ubiquitin-conjugating enzyme E2 32
PRO_0000345197

Regions

Transmembrane275 – 29521Helical; Potential

Sites

Active site931Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LSP7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B4C77186E1294D51

FASTA30934,322
        10         20         30         40         50         60 
MADERYNRKN PAVKRILQEV KEMQANPSDD FMSLPLEENI FEWQFAIRGP GDTEFEGGIY 

        70         80         90        100        110        120 
HGRIQLPADY PFKPPSFMLL TPNGRFETNT KICLSISNYH PEHWQPSWSV RTALVALIAF 

       130        140        150        160        170        180 
MPTSPNGALG SVDYPKDERR TLAIKSRETP PKYGSPERQK IIDEIHQYIL SKATVVPKPL 

       190        200        210        220        230        240 
PLECSQAPSI VSEAHSQVEP QEAITVVEER SIATTDTIVD DQIIEETAEA VNTAASVVPA 

       250        260        270        280        290        300 
AAPLPAVEVV VKASVSGEQR MARRAAQKPV DDRLFTWAAV GLTIAIMVLL LKKFIKSNGY 


STGFMDDQS 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-273, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026636 Genomic DNA. Translation: BAA94978.1.
CP002686 Genomic DNA. Translation: AEE75893.1.
AF386935 mRNA. Translation: AAK62380.1.
BT020530 mRNA. Translation: AAW52556.1.
DQ027045 mRNA. Translation: AAY44871.1.
RefSeqNP_566563.1. NM_112576.2.
UniGeneAt.6347.

3D structure databases

ProteinModelPortalQ9LSP7.
SMRQ9LSP7. Positions 10-154.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9LSP7.
PRIDEQ9LSP7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G17000.1; AT3G17000.1; AT3G17000.
GeneID820956.
KEGGath:AT3G17000.

Organism-specific databases

TAIRAT3G17000.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000167871.
InParanoidQ9LSP7.
KOK10578.
OMAFMALPLE.
PhylomeDBQ9LSP7.
ProtClustDBCLSN2688465.

Enzyme and pathway databases

BioCycARA:AT3G17000-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9LSP7.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9LSP7.

Entry information

Entry nameUBC32_ARATH
AccessionPrimary (citable) accession number: Q9LSP7
Secondary accession number(s): Q4TYX9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names