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Protein

Ubiquitin-conjugating enzyme E2 32

Gene

UBC32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 32 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 32
Ubiquitin carrier protein 32
Gene namesi
Name:UBC32
Ordered Locus Names:At3g17000
ORF Names:K14A17.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G17000
TAIRilocus:2086067 AT3G17000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451971 – 309Ubiquitin-conjugating enzyme E2 32Add BLAST309

Proteomic databases

PaxDbiQ9LSP7
PRIDEiQ9LSP7

Expressioni

Gene expression databases

ExpressionAtlasiQ9LSP7 baseline and differential
GenevisibleiQ9LSP7 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi6289, 244 interactors
STRINGi3702.AT3G17000.1

Structurei

3D structure databases

ProteinModelPortaliQ9LSP7
SMRiQ9LSP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0428 Eukaryota
COG5078 LUCA
HOGENOMiHOG000167871
InParanoidiQ9LSP7
KOiK10578
OMAiEASHREN
OrthoDBiEOG09360JM4
PhylomeDBiQ9LSP7

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LSP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADERYNRKN PAVKRILQEV KEMQANPSDD FMSLPLEENI FEWQFAIRGP
60 70 80 90 100
GDTEFEGGIY HGRIQLPADY PFKPPSFMLL TPNGRFETNT KICLSISNYH
110 120 130 140 150
PEHWQPSWSV RTALVALIAF MPTSPNGALG SVDYPKDERR TLAIKSRETP
160 170 180 190 200
PKYGSPERQK IIDEIHQYIL SKATVVPKPL PLECSQAPSI VSEAHSQVEP
210 220 230 240 250
QEAITVVEER SIATTDTIVD DQIIEETAEA VNTAASVVPA AAPLPAVEVV
260 270 280 290 300
VKASVSGEQR MARRAAQKPV DDRLFTWAAV GLTIAIMVLL LKKFIKSNGY

STGFMDDQS
Length:309
Mass (Da):34,322
Last modified:October 1, 2000 - v1
Checksum:iB4C77186E1294D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026636 Genomic DNA Translation: BAA94978.1
CP002686 Genomic DNA Translation: AEE75893.1
AF386935 mRNA Translation: AAK62380.1
BT020530 mRNA Translation: AAW52556.1
DQ027045 mRNA Translation: AAY44871.1
RefSeqiNP_566563.1, NM_112576.3
UniGeneiAt.6347

Genome annotation databases

EnsemblPlantsiAT3G17000.1; AT3G17000.1; AT3G17000
GeneIDi820956
GrameneiAT3G17000.1; AT3G17000.1; AT3G17000
KEGGiath:AT3G17000

Similar proteinsi

Entry informationi

Entry nameiUBC32_ARATH
AccessioniPrimary (citable) accession number: Q9LSP7
Secondary accession number(s): Q4TYX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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