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Q9LSP7

- UBC32_ARATH

UniProt

Q9LSP7 - UBC32_ARATH

Protein

Ubiquitin-conjugating enzyme E2 32

Gene

UBC32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei93 – 931Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G17000-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 32 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 32
    Gene namesi
    Name:UBC32
    Ordered Locus Names:At3g17000
    ORF Names:K14A17.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G17000.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: TAIR
    4. perinuclear region of cytoplasm Source: TAIR

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Ubiquitin-conjugating enzyme E2 32PRO_0000345197Add
    BLAST

    Proteomic databases

    PaxDbiQ9LSP7.
    PRIDEiQ9LSP7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LSP7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LSP7.
    SMRiQ9LSP7. Positions 14-143.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei275 – 29521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000167871.
    InParanoidiQ9LSP7.
    KOiK10578.
    OMAiFMALPLE.
    PhylomeDBiQ9LSP7.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LSP7-1 [UniParc]FASTAAdd to Basket

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    MADERYNRKN PAVKRILQEV KEMQANPSDD FMSLPLEENI FEWQFAIRGP    50
    GDTEFEGGIY HGRIQLPADY PFKPPSFMLL TPNGRFETNT KICLSISNYH 100
    PEHWQPSWSV RTALVALIAF MPTSPNGALG SVDYPKDERR TLAIKSRETP 150
    PKYGSPERQK IIDEIHQYIL SKATVVPKPL PLECSQAPSI VSEAHSQVEP 200
    QEAITVVEER SIATTDTIVD DQIIEETAEA VNTAASVVPA AAPLPAVEVV 250
    VKASVSGEQR MARRAAQKPV DDRLFTWAAV GLTIAIMVLL LKKFIKSNGY 300
    STGFMDDQS 309
    Length:309
    Mass (Da):34,322
    Last modified:October 1, 2000 - v1
    Checksum:iB4C77186E1294D51
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026636 Genomic DNA. Translation: BAA94978.1.
    CP002686 Genomic DNA. Translation: AEE75893.1.
    AF386935 mRNA. Translation: AAK62380.1.
    BT020530 mRNA. Translation: AAW52556.1.
    DQ027045 mRNA. Translation: AAY44871.1.
    RefSeqiNP_566563.1. NM_112576.2.
    UniGeneiAt.6347.

    Genome annotation databases

    EnsemblPlantsiAT3G17000.1; AT3G17000.1; AT3G17000.
    GeneIDi820956.
    KEGGiath:AT3G17000.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026636 Genomic DNA. Translation: BAA94978.1 .
    CP002686 Genomic DNA. Translation: AEE75893.1 .
    AF386935 mRNA. Translation: AAK62380.1 .
    BT020530 mRNA. Translation: AAW52556.1 .
    DQ027045 mRNA. Translation: AAY44871.1 .
    RefSeqi NP_566563.1. NM_112576.2.
    UniGenei At.6347.

    3D structure databases

    ProteinModelPortali Q9LSP7.
    SMRi Q9LSP7. Positions 14-143.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9LSP7.
    PRIDEi Q9LSP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G17000.1 ; AT3G17000.1 ; AT3G17000 .
    GeneIDi 820956.
    KEGGi ath:AT3G17000.

    Organism-specific databases

    TAIRi AT3G17000.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000167871.
    InParanoidi Q9LSP7.
    KOi K10578.
    OMAi FMALPLE.
    PhylomeDBi Q9LSP7.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT3G17000-MONOMER.

    Miscellaneous databases

    PROi Q9LSP7.

    Gene expression databases

    Genevestigatori Q9LSP7.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-273, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiUBC32_ARATH
    AccessioniPrimary (citable) accession number: Q9LSP7
    Secondary accession number(s): Q4TYX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3