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Protein

Ubiquitin-conjugating enzyme E2 32

Gene

UBC32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-like protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central
  6. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. protein ubiquitination Source: GO_Central
  2. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G17000-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 32 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 32
Gene namesi
Name:UBC32
Ordered Locus Names:At3g17000
ORF Names:K14A17.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17000.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei275 – 29521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: TAIR
  4. perinuclear region of cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Ubiquitin-conjugating enzyme E2 32PRO_0000345197Add
BLAST

Proteomic databases

PaxDbiQ9LSP7.
PRIDEiQ9LSP7.

Expressioni

Gene expression databases

GenevestigatoriQ9LSP7.

Structurei

3D structure databases

SMRiQ9LSP7. Positions 14-143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000167871.
InParanoidiQ9LSP7.
KOiK10578.
OMAiDEHAHKA.
PhylomeDBiQ9LSP7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LSP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADERYNRKN PAVKRILQEV KEMQANPSDD FMSLPLEENI FEWQFAIRGP
60 70 80 90 100
GDTEFEGGIY HGRIQLPADY PFKPPSFMLL TPNGRFETNT KICLSISNYH
110 120 130 140 150
PEHWQPSWSV RTALVALIAF MPTSPNGALG SVDYPKDERR TLAIKSRETP
160 170 180 190 200
PKYGSPERQK IIDEIHQYIL SKATVVPKPL PLECSQAPSI VSEAHSQVEP
210 220 230 240 250
QEAITVVEER SIATTDTIVD DQIIEETAEA VNTAASVVPA AAPLPAVEVV
260 270 280 290 300
VKASVSGEQR MARRAAQKPV DDRLFTWAAV GLTIAIMVLL LKKFIKSNGY

STGFMDDQS
Length:309
Mass (Da):34,322
Last modified:October 1, 2000 - v1
Checksum:iB4C77186E1294D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026636 Genomic DNA. Translation: BAA94978.1.
CP002686 Genomic DNA. Translation: AEE75893.1.
AF386935 mRNA. Translation: AAK62380.1.
BT020530 mRNA. Translation: AAW52556.1.
DQ027045 mRNA. Translation: AAY44871.1.
RefSeqiNP_566563.1. NM_112576.2.
UniGeneiAt.6347.

Genome annotation databases

EnsemblPlantsiAT3G17000.1; AT3G17000.1; AT3G17000.
GeneIDi820956.
KEGGiath:AT3G17000.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026636 Genomic DNA. Translation: BAA94978.1.
CP002686 Genomic DNA. Translation: AEE75893.1.
AF386935 mRNA. Translation: AAK62380.1.
BT020530 mRNA. Translation: AAW52556.1.
DQ027045 mRNA. Translation: AAY44871.1.
RefSeqiNP_566563.1. NM_112576.2.
UniGeneiAt.6347.

3D structure databases

SMRiQ9LSP7. Positions 14-143.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9LSP7.
PRIDEiQ9LSP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17000.1; AT3G17000.1; AT3G17000.
GeneIDi820956.
KEGGiath:AT3G17000.

Organism-specific databases

TAIRiAT3G17000.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000167871.
InParanoidiQ9LSP7.
KOiK10578.
OMAiDEHAHKA.
PhylomeDBiQ9LSP7.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT3G17000-MONOMER.

Miscellaneous databases

PROiQ9LSP7.

Gene expression databases

GenevestigatoriQ9LSP7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-273, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiUBC32_ARATH
AccessioniPrimary (citable) accession number: Q9LSP7
Secondary accession number(s): Q4TYX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.