Q9LSH2 (DCE5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate decarboxylase 5
Short name=GAD 5
EC=4.1.1.15

Gene names

Name:	GAD5
Ordered Locus Names:	At3g17760
ORF Names:	MIG5.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis By similarity.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homohexamer. Interacts with clamodulin By similarity.
Tissue specificity	Expressed in flowers. Ref.4
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Ligand	Calmodulin-binding Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Glutamate decarboxylase 5		PRO_0000416956
Amino acid modifications
	Modified residue	276	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LSH2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7985F175E54DF262	FASTA	494	55,770
	10 20 30 40 50 60 MVLATNSDSD EHLHSTFASR YVRAVVPRFK MPDHCMPKDA AYQVINDELM LDGNPRLNLA 70 80 90 100 110 120 SFVTTWMEPE CDKLIMDSVN KNYVDMDEYP VTTELQNRCV NMIANLFHAP VGEDEAAIGC 130 140 150 160 170 180 GTVGSSEAIM LAGLAFKRKW QHRRKAQGLP IDKPNIVTGA NVQVCWEKFA RYFEVELKEV 190 200 210 220 230 240 KLSEDYYVMD PAKAVEMVDE NTICVAAILG STLTGEFEDV KQLNDLLAEK NAETGWETPI 250 260 270 280 290 300 HVDAASGGFI APFLYPDLEW DFRLPWVKSI NVSGHKYGLV YAGVGWVVWR TKDDLPEELV 310 320 330 340 350 360 FHINYLGADQ PTFTLNFSKG SSQIIAQYYQ FIRLGFEGYK NIMENCMDNA RRLREGIEMT 370 380 390 400 410 420 GKFNIVSKDI GVPLVAFSLK DSSKHTVFEI AESLRKFGWI IPAYTMPADA QHIAVLRVVI 430 440 450 460 470 480 REDFSRGLAD RLITHIIQVL KEIEGLPSRI AHLAAAAAVS GDDEEVKVKT AKMSLEDITK 490 YWKRLVEHKR NIVC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Metabolism and functions of gamma-aminobutyric acid." Shelp B.J., Bown A.W., McLean M.D. Trends Plant Sci. 4:446-452(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"Contribution of the GABA shunt to hypoxia-induced alanine accumulation in roots of Arabidopsis thaliana." Miyashita Y., Good A.G. Plant Cell Physiol. 49:92-102(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026646 Genomic DNA. Translation: BAB02870.1. CP002686 Genomic DNA. Translation: AEE76003.1. CP002686 Genomic DNA. Translation: AEE76004.1.
IPI	IPI00531135.
RefSeq	NP_001154621.1. NM_001161149.1. NP_188403.1. NM_112657.1.
UniGene	At.38660.
3D structure databases
HSSP	HSSP built from PDB template 1PMM based on UniProtKB P28302.
ProteinModelPortal	Q9LSH2.
SMR	Q9LSH2. Positions 13-447.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LSH2. 1 interaction.
Proteomic databases
PRIDE	Q9LSH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G17760.1; AT3G17760.1; AT3G17760. AT3G17760.2; AT3G17760.2; AT3G17760.
GeneID	821044.
KEGG	ath:AT3G17760.
Organism-specific databases
TAIR	At3g17760.
Phylogenomic databases
InParanoid	Q9LSH2.
KO	K01580.
OMA	IELREVP.
PhylomeDB	Q9LSH2.
ProtClustDB	CLSN2683665.
Gene expression databases
Genevestigator	Q9LSH2.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCE5_ARATH

Accession

Primary (citable) accession number: Q9LSH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program