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Protein

Serine/threonine-protein kinase OXI1

Gene

OXI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxidative burst-mediated signaling. Required for basal resistance to P.parasitica infection and root hair growth. Partly required for the activation of MPK3 and MPK6 by hydrogen peroxide and cellulase elicitor.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated in response to hydrogen peroxide and cellulase elicitor. Activated by PDK1 in a phosphatidic acid dependent manner.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 31ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein kinase activity Source: TAIR
  • protein kinase binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • intracellular signal transduction Source: GO_Central
  • protein phosphorylation Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase OXI1 (EC:2.7.11.1)
Alternative name(s):
AGC serine/threonine-protein kinase subfamily 2 member 11 Publication
Protein OXIDATIVE SIGNAL-INDUCIBLE 11 Publication
Gene namesi
Name:OXI11 Publication
Synonyms:AGC2-11 Publication
Ordered Locus Names:At3g25250
ORF Names:MJL12.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G25250.
TAIRilocus:2090310. AT3G25250.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Plants have enhanced susceptibility to virulent P.parasitica pathogen and reduced root hair length.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45K → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi235S → A: Strongly reduces catalytic activity. 1 Publication1
Mutagenesisi418F → A: Loss of interaction with PDPK1 and PDK2; when associated with A-421. 1 Publication1
Mutagenesisi421F → A: Loss of interaction with PDPK1 and PDK2; when associated with A-418. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458271 – 421Serine/threonine-protein kinase OXI1Add BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235Phosphoserine; by PDPK1Curated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LSF1.

Expressioni

Tissue specificityi

Expressed in roots and root hair cells.2 Publications

Developmental stagei

Highly expressed in fast-growing organs and dividing cells.

Inductioni

By wounding, hydrogen peroxide and cellulase elicitor.1 Publication

Gene expression databases

GenevisibleiQ9LSF1. AT.

Interactioni

Subunit structurei

Interacts with PDK1 and PDK2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At2g47060O807194EBI-8574783,EBI-4436376

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi7450. 7 interactors.
ELMiQ9LSF1.
IntActiQ9LSF1. 4 interactors.
MINTiMINT-8085713.
STRINGi3702.AT3G25250.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LSF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 329Protein kinasePROSITE-ProRule annotationAdd BLAST313
Domaini330 – 421AGC-kinase C-terminalAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 246Activation loopBy similarityAdd BLAST80

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi418 – 421PIF4

Domaini

The activation loop within the kinase domain is the target of phosphorylation.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0610. Eukaryota.
ENOG410XQ0C. LUCA.
HOGENOMiHOG000233027.
InParanoidiQ9LSF1.
KOiK20714.
OMAiEYKRVSF.
OrthoDBiEOG09360GG4.
PhylomeDBiQ9LSF1.

Family and domain databases

InterProiView protein in InterPro
IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 2 hits.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS51285. AGC_KINASE_CTER. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LSF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEGDEKQSR ALDFNRLEVL SLLGRGAKGV VFLVRDDDAK LLALKVILKE
60 70 80 90 100
AIEKKKKGRE SEDDEYKRVS FEQGVLSRFD HPLFPSLHGV LATDKVIGYA
110 120 130 140 150
IDYCPGQNLN SLRKMQSESM FSDEIIRFYA AELVLALDYL HNQGIVYRDL
160 170 180 190 200
KPDNVMIQEN GHLMLIDFDL STNLAPRTPQ PSPSLSKPSP TMKRKKRLFR
210 220 230 240 250
FTSFCNSGIS PQESISVHSS STLAVSDSSG EKSNSFVGTE EYVAPEVISG
260 270 280 290 300
DGHDFAVDWW SLGVVLYEML YGATPFRGSN RKETFYRILS KPPNLTGETT
310 320 330 340 350
SLRDLIRRLL EKDPSRRINV EEIKGHDFFR GVDWEKVILV SRPPYIPAPD
360 370 380 390 400
DGGDKGTDVN TKMDVENIVQ EIFAARQERE KQSGDNNKNA NMKIKDNTSG
410 420
EWVKGLNNNH DLESDNNFLV F
Length:421
Mass (Da):47,560
Last modified:October 1, 2000 - v1
Checksum:i6855585F4DC14C4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026647 Genomic DNA. Translation: BAB02084.1.
CP002686 Genomic DNA. Translation: AEE77000.1.
BT029732 mRNA. Translation: ABM06002.1.
RefSeqiNP_189162.1. NM_113431.6.
UniGeneiAt.37401.

Genome annotation databases

EnsemblPlantsiAT3G25250.1; AT3G25250.1; AT3G25250.
GeneIDi822119.
GrameneiAT3G25250.1; AT3G25250.1; AT3G25250.
KEGGiath:AT3G25250.

Similar proteinsi

Entry informationi

Entry nameiOXI1_ARATH
AccessioniPrimary (citable) accession number: Q9LSF1
Secondary accession number(s): A1L4U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families