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Protein

Ribonucleoside-diphosphate reductase small chain C

Gene

TSO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Involved in DNA damage repair and programmed cell death inhibition.1 Publication

Miscellaneous

'TSO' means 'ugly' in Chinese.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Iron 1PROSITE-ProRule annotation1
Metal bindingi107Iron 1PROSITE-ProRule annotation1
Metal bindingi107Iron 2By similarity1
Metal bindingi110Iron 1PROSITE-ProRule annotation1
Active sitei114PROSITE-ProRule annotation1
Metal bindingi169Iron 2By similarity1
Metal bindingi203Iron 2By similarity1
Metal bindingi206Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleotide biosynthetic process Source: InterPro
  • DNA repair Source: TAIR
  • DNA replication Source: TAIR
  • multicellular organism development Source: TAIR
  • programmed cell death Source: TAIR
  • regulation of cell cycle Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G27060-MONOMER
BRENDAi1.17.4.1 399
1.17.4.2 399
ReactomeiR-ATH-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain C (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase TSO2 subunit
Ribonucleotide reductase small subunit C
Gene namesi
Name:TSO2
Ordered Locus Names:At3g27060
ORF Names:MOJ10.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G27060
TAIRilocus:2092030 AT3G27060

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryo lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49D → N in tso2-1,4; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication1
Mutagenesisi97R → C in tso2-3; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication1
Mutagenesisi170G → S in tso2-2; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002541951 – 332Ribonucleoside-diphosphate reductase small chain CAdd BLAST332

Proteomic databases

PaxDbiQ9LSD0
PRIDEiQ9LSD0

Expressioni

Tissue specificityi

Expressed in roots, cauline and rosette leaves, stems and flowers.2 Publications

Developmental stagei

Expressed predominantly at the S-phase of the cell cycle.1 Publication

Gene expression databases

ExpressionAtlasiQ9LSD0 baseline and differential
GenevisibleiQ9LSD0 AT

Interactioni

Subunit structurei

Homodimer and heterodimer with RNR2A. Heterotetramer of two R1 and two R2 chains (By similarity). Interacts with CSN7 (via C-terminal tail).By similarity1 Publication

Protein-protein interaction databases

BioGridi7654, 1 interactor
STRINGi3702.AT3G27060.1

Structurei

3D structure databases

ProteinModelPortaliQ9LSD0
SMRiQ9LSD0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
InParanoidiQ9LSD0
KOiK10808
OMAiFYVGFVQ
OrthoDBiEOG09360F7K
PhylomeDBiQ9LSD0

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LSD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSMPEEPLL TPTPDRFCMF PIHYPQIWEM YKKAEASFWT AEEVDLSQDN
60 70 80 90 100
RDWENSLNDG ERHFIKHVLA FFAASDGIVL ENLASRFMSD VQVSEARAFY
110 120 130 140 150
GFQIAIENIH SEMYSLLLDT YIKDNKERDH LFRAIETIPC VAKKAQWAMK
160 170 180 190 200
WIDGSQTFAE RIIAFACVEG IFFSGSFCSI FWLKKRGLMP GLTFSNELIS
210 220 230 240 250
RDEGLHCDFA CLLYTLLKTK LSEERVKSIV CDAVEIEREF VCDALPCALV
260 270 280 290 300
GMNRDLMSQY IEFVADRLLG ALGYGKVYGV TNPFDWMELI SLQGKTNFFE
310 320 330
KRVGDYQKAS VMSSVNGNGA FDNHVFSLDE DF
Length:332
Mass (Da):38,034
Last modified:October 1, 2000 - v1
Checksum:i9EADC6E82B4DFCE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026649 Genomic DNA Translation: BAB01087.1
CP002686 Genomic DNA Translation: AEE77261.1
AY063837 mRNA Translation: AAL36193.1
AY117212 mRNA Translation: AAM51287.1
RefSeqiNP_189342.1, NM_113620.4
UniGeneiAt.24911
At.74191

Genome annotation databases

EnsemblPlantsiAT3G27060.1; AT3G27060.1; AT3G27060
GeneIDi822324
GrameneiAT3G27060.1; AT3G27060.1; AT3G27060
KEGGiath:AT3G27060

Similar proteinsi

Entry informationi

Entry nameiRIR2C_ARATH
AccessioniPrimary (citable) accession number: Q9LSD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health