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Q9LSD0

- RIR2C_ARATH

UniProt

Q9LSD0 - RIR2C_ARATH

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Protein

Ribonucleoside-diphosphate reductase small chain C

Gene

TSO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Involved in DNA damage repair and programmed cell death inhibition.1 Publication

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Iron 1PROSITE-ProRule annotation
Metal bindingi107 – 1071Iron 1PROSITE-ProRule annotation
Metal bindingi107 – 1071Iron 2By similarity
Metal bindingi110 – 1101Iron 1PROSITE-ProRule annotation
Active sitei114 – 1141PROSITE-ProRule annotation
Metal bindingi169 – 1691Iron 2By similarity
Metal bindingi203 – 2031Iron 2By similarity
Metal bindingi206 – 2061Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA repair Source: TAIR
  3. DNA replication Source: TAIR
  4. multicellular organismal development Source: TAIR
  5. programmed cell death Source: TAIR
  6. regulation of cell cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G27060-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain C (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase TSO2 subunit
Ribonucleotide reductase small subunit C
Gene namesi
Name:TSO2
Ordered Locus Names:At3g27060
ORF Names:MOJ10.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G27060.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Cytoplasmic in adult cells, and nuclear in meristematic regions and in all tissues following DNA damage. The nuclear exclusion is dependent on CSN7.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryo lethality when homozygous.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491D → N in tso2-1,4; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication
Mutagenesisi97 – 971R → C in tso2-3; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication
Mutagenesisi170 – 1701G → S in tso2-2; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Ribonucleoside-diphosphate reductase small chain CPRO_0000254195Add
BLAST

Proteomic databases

PRIDEiQ9LSD0.

Expressioni

Tissue specificityi

Expressed in roots, cauline and rosette leaves, stems and flowers.2 Publications

Developmental stagei

Expressed predominantly at the S-phase of the cell cycle.1 Publication

Gene expression databases

ExpressionAtlasiQ9LSD0. baseline and differential.
GenevestigatoriQ9LSD0.

Interactioni

Subunit structurei

Homodimer and heterodimer with RNR2A. Heterotetramer of two R1 and two R2 chains (By similarity). Interacts with CSN7 (via C-terminal tail).By similarity1 Publication

Protein-protein interaction databases

BioGridi7654. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9LSD0.
SMRiQ9LSD0. Positions 6-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ9LSD0.
KOiK10808.
OMAiGVMNSTK.
PhylomeDBiQ9LSD0.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LSD0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSMPEEPLL TPTPDRFCMF PIHYPQIWEM YKKAEASFWT AEEVDLSQDN
60 70 80 90 100
RDWENSLNDG ERHFIKHVLA FFAASDGIVL ENLASRFMSD VQVSEARAFY
110 120 130 140 150
GFQIAIENIH SEMYSLLLDT YIKDNKERDH LFRAIETIPC VAKKAQWAMK
160 170 180 190 200
WIDGSQTFAE RIIAFACVEG IFFSGSFCSI FWLKKRGLMP GLTFSNELIS
210 220 230 240 250
RDEGLHCDFA CLLYTLLKTK LSEERVKSIV CDAVEIEREF VCDALPCALV
260 270 280 290 300
GMNRDLMSQY IEFVADRLLG ALGYGKVYGV TNPFDWMELI SLQGKTNFFE
310 320 330
KRVGDYQKAS VMSSVNGNGA FDNHVFSLDE DF
Length:332
Mass (Da):38,034
Last modified:October 1, 2000 - v1
Checksum:i9EADC6E82B4DFCE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026649 Genomic DNA. Translation: BAB01087.1.
CP002686 Genomic DNA. Translation: AEE77261.1.
AY063837 mRNA. Translation: AAL36193.1.
AY117212 mRNA. Translation: AAM51287.1.
RefSeqiNP_189342.1. NM_113620.3.
UniGeneiAt.24911.
At.74191.

Genome annotation databases

EnsemblPlantsiAT3G27060.1; AT3G27060.1; AT3G27060.
GeneIDi822324.
KEGGiath:AT3G27060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026649 Genomic DNA. Translation: BAB01087.1 .
CP002686 Genomic DNA. Translation: AEE77261.1 .
AY063837 mRNA. Translation: AAL36193.1 .
AY117212 mRNA. Translation: AAM51287.1 .
RefSeqi NP_189342.1. NM_113620.3.
UniGenei At.24911.
At.74191.

3D structure databases

ProteinModelPortali Q9LSD0.
SMRi Q9LSD0. Positions 6-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7654. 1 interaction.

Proteomic databases

PRIDEi Q9LSD0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G27060.1 ; AT3G27060.1 ; AT3G27060 .
GeneIDi 822324.
KEGGi ath:AT3G27060.

Organism-specific databases

GeneFarmi 2091. 386.
TAIRi AT3G27060.

Phylogenomic databases

InParanoidi Q9LSD0.
KOi K10808.
OMAi GVMNSTK.
PhylomeDBi Q9LSD0.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci ARA:AT3G27060-MONOMER.

Gene expression databases

ExpressionAtlasi Q9LSD0. baseline and differential.
Genevestigatori Q9LSD0.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development."
    Wang C., Liu Z.
    Plant Cell 18:350-365(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF ASP-49; ARG-97 AND GLY-170, DEVELOPMENTAL STAGE.
    Strain: cv. Landsberg erecta.
  5. "COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)."
    Halimi Y., Dessau M., Pollak S., Ast T., Erez T., Livnat-Levanon N., Karniol B., Hirsch J.A., Chamovitz D.A.
    Plant Mol. Biol. 77:77-89(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CSN7, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRIR2C_ARATH
AccessioniPrimary (citable) accession number: Q9LSD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'TSO' means 'ugly' in Chinese.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3