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Q9LSD0

- RIR2C_ARATH

UniProt

Q9LSD0 - RIR2C_ARATH

Protein

Ribonucleoside-diphosphate reductase small chain C

Gene

TSO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Involved in DNA damage repair and programmed cell death inhibition.1 Publication

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761Iron 1PROSITE-ProRule annotation
    Metal bindingi107 – 1071Iron 1PROSITE-ProRule annotation
    Metal bindingi107 – 1071Iron 2By similarity
    Metal bindingi110 – 1101Iron 1PROSITE-ProRule annotation
    Active sitei114 – 1141PROSITE-ProRule annotation
    Metal bindingi169 – 1691Iron 2By similarity
    Metal bindingi203 – 2031Iron 2By similarity
    Metal bindingi206 – 2061Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA repair Source: TAIR
    3. DNA replication Source: TAIR
    4. multicellular organismal development Source: TAIR
    5. programmed cell death Source: TAIR
    6. regulation of cell cycle Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G27060-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain C (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase TSO2 subunit
    Ribonucleotide reductase small subunit C
    Gene namesi
    Name:TSO2
    Ordered Locus Names:At3g27060
    ORF Names:MOJ10.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G27060.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Cytoplasmic in adult cells, and nuclear in meristematic regions and in all tissues following DNA damage. The nuclear exclusion is dependent on CSN7.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethality when homozygous.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi49 – 491D → N in tso2-1,4; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication
    Mutagenesisi97 – 971R → C in tso2-3; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication
    Mutagenesisi170 – 1701G → S in tso2-2; white sectors in green organs, uneven thickness, rough surfaces, and irregular margins of leaves or floral organs. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Ribonucleoside-diphosphate reductase small chain CPRO_0000254195Add
    BLAST

    Proteomic databases

    PRIDEiQ9LSD0.

    Expressioni

    Tissue specificityi

    Expressed in roots, cauline and rosette leaves, stems and flowers.2 Publications

    Developmental stagei

    Expressed predominantly at the S-phase of the cell cycle.1 Publication

    Gene expression databases

    ArrayExpressiQ9LSD0.
    GenevestigatoriQ9LSD0.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer with RNR2A. Heterotetramer of two R1 and two R2 chains By similarity. Interacts with CSN7 (via C-terminal tail).By similarity1 Publication

    Protein-protein interaction databases

    BioGridi7654. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LSD0.
    SMRiQ9LSD0. Positions 6-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    InParanoidiQ9LSD0.
    KOiK10808.
    OMAiGVMNSTK.
    PhylomeDBiQ9LSD0.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LSD0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSMPEEPLL TPTPDRFCMF PIHYPQIWEM YKKAEASFWT AEEVDLSQDN    50
    RDWENSLNDG ERHFIKHVLA FFAASDGIVL ENLASRFMSD VQVSEARAFY 100
    GFQIAIENIH SEMYSLLLDT YIKDNKERDH LFRAIETIPC VAKKAQWAMK 150
    WIDGSQTFAE RIIAFACVEG IFFSGSFCSI FWLKKRGLMP GLTFSNELIS 200
    RDEGLHCDFA CLLYTLLKTK LSEERVKSIV CDAVEIEREF VCDALPCALV 250
    GMNRDLMSQY IEFVADRLLG ALGYGKVYGV TNPFDWMELI SLQGKTNFFE 300
    KRVGDYQKAS VMSSVNGNGA FDNHVFSLDE DF 332
    Length:332
    Mass (Da):38,034
    Last modified:October 1, 2000 - v1
    Checksum:i9EADC6E82B4DFCE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026649 Genomic DNA. Translation: BAB01087.1.
    CP002686 Genomic DNA. Translation: AEE77261.1.
    AY063837 mRNA. Translation: AAL36193.1.
    AY117212 mRNA. Translation: AAM51287.1.
    RefSeqiNP_189342.1. NM_113620.3.
    UniGeneiAt.24911.
    At.74191.

    Genome annotation databases

    EnsemblPlantsiAT3G27060.1; AT3G27060.1; AT3G27060.
    GeneIDi822324.
    KEGGiath:AT3G27060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026649 Genomic DNA. Translation: BAB01087.1 .
    CP002686 Genomic DNA. Translation: AEE77261.1 .
    AY063837 mRNA. Translation: AAL36193.1 .
    AY117212 mRNA. Translation: AAM51287.1 .
    RefSeqi NP_189342.1. NM_113620.3.
    UniGenei At.24911.
    At.74191.

    3D structure databases

    ProteinModelPortali Q9LSD0.
    SMRi Q9LSD0. Positions 6-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 7654. 1 interaction.

    Proteomic databases

    PRIDEi Q9LSD0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G27060.1 ; AT3G27060.1 ; AT3G27060 .
    GeneIDi 822324.
    KEGGi ath:AT3G27060.

    Organism-specific databases

    GeneFarmi 2091. 386.
    TAIRi AT3G27060.

    Phylogenomic databases

    InParanoidi Q9LSD0.
    KOi K10808.
    OMAi GVMNSTK.
    PhylomeDBi Q9LSD0.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci ARA:AT3G27060-MONOMER.

    Gene expression databases

    ArrayExpressi Q9LSD0.
    Genevestigatori Q9LSD0.

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development."
      Wang C., Liu Z.
      Plant Cell 18:350-365(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF ASP-49; ARG-97 AND GLY-170, DEVELOPMENTAL STAGE.
      Strain: cv. Landsberg erecta.
    5. "COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)."
      Halimi Y., Dessau M., Pollak S., Ast T., Erez T., Livnat-Levanon N., Karniol B., Hirsch J.A., Chamovitz D.A.
      Plant Mol. Biol. 77:77-89(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSN7, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRIR2C_ARATH
    AccessioniPrimary (citable) accession number: Q9LSD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    'TSO' means 'ugly' in Chinese.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3