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Protein

Protein ASPARTIC PROTEASE IN GUARD CELL 1

Gene

ASPG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Aspartic protease involved in drought avoidance through abscisic acid signaling.1 Publication

Enzyme regulationi

Inhibited by pepstatin A.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801PROSITE-ProRule annotation
Active sitei379 – 3791PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • protein catabolic process Source: GO_Central
  • response to abscisic acid Source: TAIR
  • response to water deprivation Source: TAIR
  • systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciARA:AT3G18490-MONOMER.

Protein family/group databases

MEROPSiA01.A09.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ASPARTIC PROTEASE IN GUARD CELL 1 (EC:3.4.23.-)
Short name:
AtASPG1
Gene namesi
Name:ASPG1
Ordered Locus Names:At3g18490
ORF Names:MYF24.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G18490.

Subcellular locationi

  • Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

No effect on stomatal closure.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi180 – 1801D → N: Loss of protease activity. Loss of protease activity; when associated with N-379. 1 Publication
Mutagenesisi379 – 3791D → N: Loss of protease activity. Loss of protease activity; when associated with N-180. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 500476Protein ASPARTIC PROTEASE IN GUARD CELL 1PRO_0000417495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi190 ↔ 193By similarity
Disulfide bondi196 ↔ 271By similarity
Disulfide bondi217 ↔ 235By similarity
Disulfide bondi222 ↔ 230By similarity
Disulfide bondi307 ↔ 500By similarity
Disulfide bondi419 ↔ 461By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9LS40.
PRIDEiQ9LS40.

PTM databases

iPTMnetiQ9LS40.
SwissPalmiQ9LS40.

Expressioni

Tissue specificityi

Expressed in young seedlings, leaves, guard-cells, stems, flowers and siliques, but not in roots or mesophyll cells.1 Publication

Inductioni

Up-regulated by abscisic acid and drought.1 Publication

Gene expression databases

GenevisibleiQ9LS40. AT.

Interactioni

Protein-protein interaction databases

BioGridi6712. 1 interaction.
IntActiQ9LS40. 1 interaction.
STRINGi3702.AT3G18490.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LS40.
SMRiQ9LS40. Positions 161-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 496335Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000237482.
InParanoidiQ9LS40.
OMAiVASQHKD.
OrthoDBiEOG093609K8.
PhylomeDBiQ9LS40.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033148. ASPG1.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 3 hits.
PTHR13683:SF377. PTHR13683:SF377. 3 hits.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LS40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPRFLSLL AVVTLSLFLT TTDASSRSLS TPPKTNVLDV VSSLQQTQTI
60 70 80 90 100
LSLDPTRSSL TTTKPESLSD PVFFNSSSPL SLELHSRDTF VASQHKDYKS
110 120 130 140 150
LTLSRLERDS SRVAGIVAKI RFAVEGVDRS DLKPVYNEDT RYQTEDLTTP
160 170 180 190 200
VVSGASQGSG EYFSRIGVGT PAKEMYLVLD TGSDVNWIQC EPCADCYQQS
210 220 230 240 250
DPVFNPTSSS TYKSLTCSAP QCSLLETSAC RSNKCLYQVS YGDGSFTVGE
260 270 280 290 300
LATDTVTFGN SGKINNVALG CGHDNEGLFT GAAGLLGLGG GVLSITNQMK
310 320 330 340 350
ATSFSYCLVD RDSGKSSSLD FNSVQLGGGD ATAPLLRNKK IDTFYYVGLS
360 370 380 390 400
GFSVGGEKVV LPDAIFDVDA SGSGGVILDC GTAVTRLQTQ AYNSLRDAFL
410 420 430 440 450
KLTVNLKKGS SSISLFDTCY DFSSLSTVKV PTVAFHFTGG KSLDLPAKNY
460 470 480 490 500
LIPVDDSGTF CFAFAPTSSS LSIIGNVQQQ GTRITYDLSK NVIGLSGNKC
Length:500
Mass (Da):53,234
Last modified:October 1, 2000 - v1
Checksum:iF5DF2E1CF9CB306C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741E → D in AAL87345 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026658 Genomic DNA. Translation: BAB01116.1.
CP002686 Genomic DNA. Translation: AEE76104.1.
AY150497 mRNA. Translation: AAN13013.1.
AY080874 mRNA. Translation: AAL87345.1.
RefSeqiNP_188478.1. NM_112734.2.
UniGeneiAt.22647.
At.71546.

Genome annotation databases

EnsemblPlantsiAT3G18490.1; AT3G18490.1; AT3G18490.
GeneIDi821379.
GrameneiAT3G18490.1; AT3G18490.1; AT3G18490.
KEGGiath:AT3G18490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026658 Genomic DNA. Translation: BAB01116.1.
CP002686 Genomic DNA. Translation: AEE76104.1.
AY150497 mRNA. Translation: AAN13013.1.
AY080874 mRNA. Translation: AAL87345.1.
RefSeqiNP_188478.1. NM_112734.2.
UniGeneiAt.22647.
At.71546.

3D structure databases

ProteinModelPortaliQ9LS40.
SMRiQ9LS40. Positions 161-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6712. 1 interaction.
IntActiQ9LS40. 1 interaction.
STRINGi3702.AT3G18490.1.

Protein family/group databases

MEROPSiA01.A09.

PTM databases

iPTMnetiQ9LS40.
SwissPalmiQ9LS40.

Proteomic databases

PaxDbiQ9LS40.
PRIDEiQ9LS40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G18490.1; AT3G18490.1; AT3G18490.
GeneIDi821379.
GrameneiAT3G18490.1; AT3G18490.1; AT3G18490.
KEGGiath:AT3G18490.

Organism-specific databases

TAIRiAT3G18490.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000237482.
InParanoidiQ9LS40.
OMAiVASQHKD.
OrthoDBiEOG093609K8.
PhylomeDBiQ9LS40.

Enzyme and pathway databases

BioCyciARA:AT3G18490-MONOMER.

Miscellaneous databases

PROiQ9LS40.

Gene expression databases

GenevisibleiQ9LS40. AT.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033148. ASPG1.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 3 hits.
PTHR13683:SF377. PTHR13683:SF377. 3 hits.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASPG1_ARATH
AccessioniPrimary (citable) accession number: Q9LS40
Secondary accession number(s): Q8RXI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.