Q9LS02 (AOC2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allene oxide cyclase 2, chloroplastic
EC=5.3.99.6

Gene names

Name:	AOC2
Ordered Locus Names:	At3g25770
ORF Names:	K13N2.11, K13N2_9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the production of 12-oxo-phytodienoic acid (OPDA), a precursor of jasmonic acid.
Catalytic activity	(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate.
Subcellular location	Plastid › chloroplast Ref.1.
Tissue specificity	Highly expressed in fully developed leaves. Ref.1
Induction	High local and systemic induction by wounding. Ref.1
Miscellaneous	The four allene oxide cyclase proteins (AOC1, AOC2, AOC3 and AOC4) are encoded by duplicated genes. They are very similar, and most experiments involving antibodies do not discriminate between the different members.
Sequence similarities	Belongs to the allene oxide cyclase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	jasmonic acid biosynthetic process Traceable author statement PubMed 11891244. Source: TAIR response to cold Inferred from expression pattern PubMed 16923014. Source: TAIR
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931PubMed 20061580. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 18633119PubMed 20061580. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR stromule Inferred from direct assay PubMed 16923014. Source: TAIR
Molecular_function	allene-oxide cyclase activity Inferred from direct assay PubMed 17085685. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 77

77

Chloroplast Potential

Chain

78 – 253

176

Allene oxide cyclase 2, chloroplastic

PRO_0000001703

Secondary structure

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253

Helix Strand Turn

Details...

Beta strand

82 – 89

8

Beta strand

104 – 108

5

Beta strand

113 – 116

4

Beta strand

119 – 122

4

Beta strand

128 – 142

15

Helix

143 – 145

3

Beta strand

147 – 157

11

Helix

159 – 161

3

Beta strand

162 – 171

10

Beta strand

176 – 185

10

Turn

186 – 189

4

Beta strand

191 – 200

10

Turn

201 – 203

3

Beta strand

204 – 212

9

Helix

220 – 222

3

Helix

237 – 240

4

Helix

244 – 246

3

Sequences

	Sequence		Length	Mass (Da)
	Q9LS02 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1E735E6AF9EBDBAC	FASTA	253	27,635
	10 20 30 40 50 60 MASSAVSLQS ISMTTLNNLS CNQQFHRSSL LGSSKSFQNL GISSNGSDFS YPSSFTAKKN 70 80 90 100 110 120 LTASRALSQN GNIENPRPSK VQELSVYEIN ELDRHSPKIL KNAFSLMFGL GDLVPFTNKL 130 140 150 160 170 180 YTGDLKKRVG ITAGLCVVIE HVPEKKGERF EATYSFYFGD YGHLSVQGPY LTYEDSFLAI 190 200 210 220 230 240 TGGAGIFEGA YGQVKLQQLV YPTKLFYTFY LKGLANDLPL ELTGTPVPPS KDIEPAPEAK 250 ALEPSGVISN YTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana." Stenzel I., Hause B., Miersch O., Kurz T., Maucher H., Weichart H., Ziegler J., Feussner I., Wasternack C. Plant Mol. Biol. 51:895-911(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"X-ray structure of allene oxide cyclase from Arabidopsis thaliana At3g25770." Center for eukaryotic structural genomics (CESG) Submitted (APR-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS) OF 62-253.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ308484 mRNA. Translation: CAC83762.1.
AB028607 Genomic DNA. Translation: BAA95764.1.
CP002686 Genomic DNA. Translation: AEE77066.1.
AY054131 mRNA. Translation: AAL06792.1.
AF380630 mRNA. Translation: AAK55711.1.

IPI

IPI00520951.

RefSeq

NP_566776.1. NM_113476.3.

UniGene

At.6411.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z8K	X-ray	1.71	A/B/C	62-253	[»]
2BRJ	X-ray	1.50	A/B/C	78-253	[»]
2DIO	X-ray	1.70	A/B/C	78-253	[»]
2GIN	X-ray	1.80	A/B/C/D/E/F	78-253	[»]
2Q4I	X-ray	1.71	A/B/C	62-253	[»]

ProteinModelPortal

Q9LS02.

SMR

Q9LS02. Positions 80-253.

ModBase

Search...

Protein-protein interaction databases

STRING

3702.AT3G25770.1-P.

Proteomic databases

PaxDb

Q9LS02.

PRIDE

Q9LS02.

Protocols and materials databases

DNASU

822168.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G25770.1; AT3G25770.1; AT3G25770.

GeneID

822168.

KEGG

ath:AT3G25770.

Organism-specific databases

GeneFarm

2623. 242.

TAIR

At3g25770.

Phylogenomic databases

eggNOG

NOG312289.

HOGENOM

HOG000240167.

InParanoid

Q9LS02.

KO

K10525.

OMA

NIENPRP.

PhylomeDB

Q9LS02.

ProtClustDB

PLN02343.

Enzyme and pathway databases

BioCyc

MetaCyc:AT3G25770-MONOMER.

Gene expression databases

Genevestigator

Q9LS02.

GermOnline

AT3G25770. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR009410. Allene_ox_cyc.
[Graphical view]

Pfam

PF06351. Allene_ox_cyc. 1 hit.
[Graphical view]

ProDom

PD329160. Allene_ox_cyc. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9LS02.

Entry information

Entry name

AOC2_ARATH

Accession

Primary (citable) accession number: Q9LS02

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program