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Protein

Phospholipase D zeta 1

Gene

PLDZETA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Phosphatidylcholine-selective (PubMed:11891260). Regulates root-hair morphogenesis (PubMed:12775839). Contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis in phosphorus-starved roots (PubMed:16891548). Involved in root elongation during phosphate limitation (PubMed:16384909).4 Publications

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.1 Publication

Cofactori

Note: Does not require Ca2+ or any other cation for activity.1 Publication

Enzyme regulationi

Calcium-independent and PIP2-dependent.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei482PROSITE-ProRule annotation1
Active sitei484PROSITE-ProRule annotation1
Active sitei489PROSITE-ProRule annotation1
Active sitei897PROSITE-ProRule annotation1
Active sitei899PROSITE-ProRule annotation1
Active sitei904PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G16785-MONOMER.
MetaCyc:AT3G16785-MONOMER.
BRENDAi3.1.4.4. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-2029485. Role of phospholipids in phagocytosis.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D zeta 11 Publication (EC:3.1.4.41 Publication)
Short name:
PLDzeta11 Publication
Alternative name(s):
Phospholipase D p1
Short name:
AtPLDp1
Phospholipase D1 PHOX and PX-containing domain protein
Gene namesi
Name:PLDZETA11 Publication
Synonyms:PLDP1
Ordered Locus Names:At3g16785, At3g16790Imported
ORF Names:K20I9.1Imported, MGL6.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G16785.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions (PubMed:16384909). No effect on root hair patterning or root hair growth (PubMed:16384909). No effect on the concentration of phospholipids and galactolipids in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show a smaller decrease in phosphatidylcholine and a smaller increase in digalactosyldiacylglycerol in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show reduced primary root elongation and increased lateral root elongation under low-phosphate conditions (PubMed:16384909).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002188182 – 1096Phospholipase D zeta 1Add BLAST1095

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LRZ5.

PTM databases

iPTMnetiQ9LRZ5.

Expressioni

Tissue specificityi

Expressed in inflorescences, flowers, siliques, stems, leaves, and roots. Highest expression in roots.1 Publication

Inductioni

Transcriptionally regulated by GL2 (PubMed:12775839). Up-regulated by phosphate limitation (PubMed:16384909).2 Publications

Gene expression databases

GenevisibleiQ9LRZ5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G16785.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LRZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 204PXAdd BLAST155
Domaini234 – 342PHPROSITE-ProRule annotationAdd BLAST109
Domaini477 – 504PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST28
Domaini892 – 919PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000246972.
InParanoidiQ9LRZ5.
KOiK01115.
OMAiDWVASIN.
OrthoDBiEOG093600S3.
PhylomeDBiQ9LRZ5.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 3 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LRZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEQLMSPA SGGGRYFQMQ PEQFPSMVSS LFSFAPAPTQ ETNRIFEELP
60 70 80 90 100
KAVIVSVSRP DAGDISPVLL SYTIECQYKQ FKWQLVKKAS QVFYLHFALK
110 120 130 140 150
KRAFIEEIHE KQEQVKEWLQ NLGIGDHPPV VQDEDADEVP LHQDESAKNR
160 170 180 190 200
DVPSSAALPV IRPLGRQQSI SVRGKHAMQE YLNHFLGNLD IVNSREVCRF
210 220 230 240 250
LEVSMLSFSP EYGPKLKEDY IMVKHLPKFS KSDDDSNRCC GCCWFCCCND
260 270 280 290 300
NWQKVWGVLK PGFLALLEDP FDAKLLDIIV FDVLPVSNGN DGVDISLAVE
310 320 330 340 350
LKDHNPLRHA FKVTSGNRSI RIRAKNSAKV KDWVASINDA ALRPPEGWCH
360 370 380 390 400
PHRFGSYAPP RGLTDDGSQA QWFVDGGAAF AAIAAAIENA KSEIFICGWW
410 420 430 440 450
VCPELYLRRP FDPHTSSRLD NLLENKAKQG VQIYILIYKE VALALKINSV
460 470 480 490 500
YSKRRLLGIH ENVRVLRYPD HFSSGVYLWS HHEKLVIVDN QVCFIGGLDL
510 520 530 540 550
CFGRYDTFEH KVGDNPSVTW PGKDYYNPRE SEPNTWEDAL KDELERKKHP
560 570 580 590 600
RMPWHDVHCA LWGPPCRDVA RHFVQRWNYA KRNKAPYEDS IPLLMPQHHM
610 620 630 640 650
VIPHYMGRQE ESDIESKKEE DSIRGIRRDD SFSSRSSLQD IPLLLPHEPV
660 670 680 690 700
DQDGSSGGHK ENGTNNRNGP FSFRKSKIEP VDGDTPMRGF VDDRNGLDLP
710 720 730 740 750
VAKRGSNAID SEWWETQDHD YQVGSPDETG QVGPRTSCRC QIIRSVSQWS
760 770 780 790 800
AGTSQVEESI HSAYRSLIDK AEHFIYIENQ FFISGLSGDD TVKNRVLEAL
810 820 830 840 850
YKRILRAHNE KKIFRVVVVI PLLPGFQGGI DDSGAASVRA IMHWQYRTIY
860 870 880 890 900
RGHNSILTNL YNTIGVKAHD YISFYGLRAY GKLSEDGPVA TSQVYVHSKI
910 920 930 940 950
MIVDDRAALI GSANINDRSL LGSRDSEIGV LIEDTELVDS RMAGKPWKAG
960 970 980 990 1000
KFSSSLRLSL WSEHLGLRTG EIDQIIDPVS DSTYKEIWMA TAKTNTMIYQ
1010 1020 1030 1040 1050
DVFSCVPNDL IHSRMAFRQS LSYWKEKLGH TTIDLGIAPE KLESYHNGDI
1060 1070 1080 1090
KRSDPMDRLK AIKGHLVSFP LDFMCKEDLR PVFNESEYYA SPQVFH
Length:1,096
Mass (Da):124,505
Last modified:October 1, 2000 - v1
Checksum:i8B71D9D0DDEAEA45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411833 mRNA. Translation: AAL06337.1.
AB028608, AB022217 Genomic DNA. Translation: BAA95772.2.
CP002686 Genomic DNA. Translation: AEE75865.1.
RefSeqiNP_188302.2. NM_112553.4.
UniGeneiAt.21958.

Genome annotation databases

EnsemblPlantsiAT3G16785.1; AT3G16785.1; AT3G16785.
GeneIDi820932.
GrameneiAT3G16785.1; AT3G16785.1; AT3G16785.
KEGGiath:AT3G16785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411833 mRNA. Translation: AAL06337.1.
AB028608, AB022217 Genomic DNA. Translation: BAA95772.2.
CP002686 Genomic DNA. Translation: AEE75865.1.
RefSeqiNP_188302.2. NM_112553.4.
UniGeneiAt.21958.

3D structure databases

ProteinModelPortaliQ9LRZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G16785.1.

PTM databases

iPTMnetiQ9LRZ5.

Proteomic databases

PaxDbiQ9LRZ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G16785.1; AT3G16785.1; AT3G16785.
GeneIDi820932.
GrameneiAT3G16785.1; AT3G16785.1; AT3G16785.
KEGGiath:AT3G16785.

Organism-specific databases

TAIRiAT3G16785.

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000246972.
InParanoidiQ9LRZ5.
KOiK01115.
OMAiDWVASIN.
OrthoDBiEOG093600S3.
PhylomeDBiQ9LRZ5.

Enzyme and pathway databases

BioCyciARA:AT3G16785-MONOMER.
MetaCyc:AT3G16785-MONOMER.
BRENDAi3.1.4.4. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-2029485. Role of phospholipids in phagocytosis.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9LRZ5.

Gene expression databases

GenevisibleiQ9LRZ5. AT.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 3 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLDZ1_ARATH
AccessioniPrimary (citable) accession number: Q9LRZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.