Q9LRZ4 (P2C41_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 41
Short name=AtPP2C41
EC=3.1.3.16

Gene names

Ordered Locus Names:	At3g16800
ORF Names:	K20I9.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from direct assay PubMed 15610358. Source: TAIR nucleus Inferred from direct assay PubMed 15610358. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LRZ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LRZ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.
     295-315: MWDVMTNNEAVEIVRGVKERR → VIYQTYSAFKSKSRLINHIIV
     316-351: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Probable protein phosphatase 2C 41		PRO_0000367965
Regions
	Domain	169 – 309	141	PP2C-like
Sites
	Metal binding	98	1	Manganese 1 By similarity
	Metal binding	98	1	Manganese 2 By similarity
	Metal binding	99	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	293	1	Manganese 2 By similarity
	Metal binding	339	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	1 – 95	95	Missing in isoform 2.		VSP_036767
	Alternative sequence	295 – 315	21	MWDVM…VKERR → VIYQTYSAFKSKSRLINHII V in isoform 2.		VSP_036768
	Alternative sequence	316 – 351	36	Missing in isoform 2.		VSP_036769

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5161F2EA4314BFD6	FASTA	351	38,601
	10 20 30 40 50 60 MVLLPAFLDG LARTVSTKKG KKLSEDEDGG REIAKSMIKD SKKNSTLLGT SGFVSSESSK 70 80 90 100 110 120 RFTSICSNRG EKGINQDRAI VWEGFGCQED ITFCGMFDGH GPWGHVIAKR VKKSFPSSLL 130 140 150 160 170 180 CQWQQTLASL SSSPECSSPF DLWKQACLKT FSIIDLDLKI SPSIDSYCSG CTALTAVLQG 190 200 210 220 230 240 DHLVIANAGD SRAVIATTSD DGNGLVPVQL SVDFKPNIPE EAERIKQSDG RLFCLDDEPG 250 260 270 280 290 300 VYRVGMPNGG SLGLAVSRAF GDYCLKDFGL VSEPEVTYRK ITDKDQFLIL ATDGMWDVMT 310 320 330 340 350 NNEAVEIVRG VKERRKSAKR LVERAVTLWR RKRRSIAMDD ISVLCLFFRP S
	Isoform 2 [UniParc]. Checksum: 8581675EA34F603A	FASTA	220	23,983
	10 20 30 40 50 60 MFDGHGPWGH VIAKRVKKSF PSSLLCQWQQ TLASLSSSPE CSSPFDLWKQ ACLKTFSIID 70 80 90 100 110 120 LDLKISPSID SYCSGCTALT AVLQGDHLVI ANAGDSRAVI ATTSDDGNGL VPVQLSVDFK 130 140 150 160 170 180 PNIPEEAERI KQSDGRLFCL DDEPGVYRVG MPNGGSLGLA VSRAFGDYCL KDFGLVSEPE 190 200 210 220 VTYRKITDKD QFLILATDGV IYQTYSAFKS KSRLINHIIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028608 Genomic DNA. Translation: BAA95773.1. CP002686 Genomic DNA. Translation: AEE75866.1. CP002686 Genomic DNA. Translation: AEE75867.1. AY099831 mRNA. Translation: AAM20682.1. BT000321 mRNA. Translation: AAN15640.1. AK317121 mRNA. Translation: BAH19809.1. AY088376 mRNA. Translation: AAM65915.1.
IPI	IPI00528853. IPI00545994.
RefSeq	NP_188303.1. NM_112554.2. NP_850599.2. NM_180268.3.
UniGene	At.38836.
3D structure databases
ProteinModelPortal	Q9LRZ4.
SMR	Q9LRZ4. Positions 62-349.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G16800.1; AT3G16800.1; AT3G16800. AT3G16800.2; AT3G16800.2; AT3G16800.
GeneID	820933.
KEGG	ath:AT3G16800.
Organism-specific databases
TAIR	At3g16800.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233897.
InParanoid	Q9LRZ4.
OMA	FGCQEDI.
PhylomeDB	Q9LRZ4.
ProtClustDB	CLSN2684170.
Gene expression databases
ArrayExpress	Q9LRZ4.
Genevestigator	Q9LRZ4.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C41_ARATH

Accession

Primary (citable) accession number: Q9LRZ4
Secondary accession number(s): Q8LPG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program