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Protein

(S)-2-hydroxy-acid oxidase GLO2

Gene

GLO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24SubstratePROSITE-ProRule annotation1
Binding sitei106FMNPROSITE-ProRule annotation1
Binding sitei127FMNPROSITE-ProRule annotation1
Binding sitei129SubstratePROSITE-ProRule annotation1
Binding sitei155FMNPROSITE-ProRule annotation1
Binding sitei164SubstratePROSITE-ProRule annotation1
Binding sitei230FMNPROSITE-ProRule annotation1
Active sitei254Proton acceptorPROSITE-ProRule annotation1
Binding sitei257SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 309FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolate pathway, Photorespiration
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14415-MONOMER
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
R-ATH-390918 Peroxisomal lipid metabolism
UniPathwayiUPA00951; UER00912

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-hydroxy-acid oxidase GLO2 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 1
Short name:
AtGLO2
Short name:
GOX 1
Short chain alpha-hydroxy acid oxidase GLO2
Gene namesi
Name:GLO2
Synonyms:GOX1
Ordered Locus Names:At3g14415
ORF Names:MLN21.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G14415

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002063231 – 367(S)-2-hydroxy-acid oxidase GLO2Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LRS0
PRIDEiQ9LRS0

PTM databases

iPTMnetiQ9LRS0

Expressioni

Gene expression databases

ExpressionAtlasiQ9LRS0 baseline and differential
GenevisibleiQ9LRS0 AT

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

BioGridi5998, 5 interactors
IntActiQ9LRS0, 1 interactor
STRINGi3702.AT3G14415.2

Structurei

3D structure databases

ProteinModelPortaliQ9LRS0
SMRiQ9LRS0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST359

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538 Eukaryota
COG1304 LUCA
HOGENOMiHOG000217463
InParanoidiQ9LRS0
KOiK11517
PhylomeDBiQ9LRS0

Family and domain databases

CDDicd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012133 Alpha-hydoxy_acid_DH_FMN
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
PfamiView protein in Pfam
PF01070 FMN_dh, 1 hit
PIRSFiPIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEiView protein in PROSITE
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LRS0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEITNVTEYD AIAKAKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR
60 70 80 90 100
ILIDVNKIDM ATTVLGFKIS MPIMVAPTAF QKMAHPDGEY ATARAASAAG
110 120 130 140 150
TIMTLSSWAT SSVEEVASTG PGIRFFQLYV YKNRKVVEQL VRRAEKAGFK
160 170 180 190 200
AIALTVDTPR LGRRESDIKN RFTLPPNLTL KNFEGLDLGK MDEANDSGLA
210 220 230 240 250
SYVAGQIDRT LSWKDIQWLQ TITNMPILVK GVLTGEDARI AIQAGAAGII
260 270 280 290 300
VSNHGARQLD YVPATISALE EVVKATQGRV PVFLDGGVRR GTDVFKALAL
310 320 330 340 350
GASGIFIGRP VVFALAAEGE AGVKKVLQML RDEFELTMAL SGCRSLSEIT
360
RNHIVTEWDT PRHLPRL
Length:367
Mass (Da):40,307
Last modified:October 1, 2000 - v1
Checksum:iC0B0F9B083F1B6E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028617, AB022220 Genomic DNA Translation: BAB01333.1
CP002686 Genomic DNA Translation: AEE75515.1
CP002686 Genomic DNA Translation: AEE75517.1
AY058095 mRNA Translation: AAL24203.1
AY136402 mRNA Translation: AAM97068.1
BT002129 mRNA Translation: AAN72140.1
AK221716 mRNA Translation: BAD95441.1
RefSeqiNP_001189892.1, NM_001202963.1 [Q9LRS0-1]
NP_188059.1, NM_112301.3 [Q9LRS0-1]
UniGeneiAt.20363
At.67927

Genome annotation databases

EnsemblPlantsiAT3G14415.1; AT3G14415.1; AT3G14415 [Q9LRS0-1]
AT3G14415.3; AT3G14415.3; AT3G14415 [Q9LRS0-1]
GeneIDi820664
GrameneiAT3G14415.1; AT3G14415.1; AT3G14415 [Q9LRS0-1]
AT3G14415.3; AT3G14415.3; AT3G14415 [Q9LRS0-1]
KEGGiath:AT3G14415

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLO2_ARATH
AccessioniPrimary (citable) accession number: Q9LRS0
Secondary accession number(s): Q56XF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health