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Q9LRS0

- GLO2_ARATH

UniProt

Q9LRS0 - GLO2_ARATH

Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO2

Gene

GLO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241SubstratePROSITE-ProRule annotation
    Binding sitei106 – 1061FMNPROSITE-ProRule annotation
    Binding sitei127 – 1271FMNPROSITE-ProRule annotation
    Binding sitei129 – 1291SubstratePROSITE-ProRule annotation
    Binding sitei155 – 1551FMNPROSITE-ProRule annotation
    Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
    Binding sitei230 – 2301FMNPROSITE-ProRule annotation
    Active sitei254 – 2541Proton acceptorPROSITE-ProRule annotation
    Binding sitei257 – 2571SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi285 – 30925FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    3. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    4. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolate pathway, Photorespiration

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciARA:AT3G14415-MONOMER.
    ARA:GQT-740-MONOMER.
    ARA:GQT-741-MONOMER.
    UniPathwayiUPA00951; UER00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (EC:1.1.3.15)
    Alternative name(s):
    Glycolate oxidase 1
    Short name:
    AtGLO2
    Short name:
    GOX 1
    Short chain alpha-hydroxy acid oxidase GLO2
    Gene namesi
    Name:GLO2
    Synonyms:GOX1
    Ordered Locus Names:At3g14415
    ORF Names:MLN21.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G14415.

    Subcellular locationi

    Peroxisome 2 Publications

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Peroxisomal (S)-2-hydroxy-acid oxidase GLO2PRO_0000206323Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9LRS0.
    PRIDEiQ9LRS0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LRS0.

    Interactioni

    Subunit structurei

    Homotetramer or homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi5998. 5 interactions.
    IntActiQ9LRS0. 1 interaction.
    STRINGi3702.AT3G14415.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LRS0.
    SMRiQ9LRS0. Positions 1-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 359359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi365 – 3673Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    HOGENOMiHOG000217463.
    InParanoidiQ9LRS0.
    KOiK11517.
    PhylomeDBiQ9LRS0.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9LRS0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEITNVTEYD AIAKAKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR    50
    ILIDVNKIDM ATTVLGFKIS MPIMVAPTAF QKMAHPDGEY ATARAASAAG 100
    TIMTLSSWAT SSVEEVASTG PGIRFFQLYV YKNRKVVEQL VRRAEKAGFK 150
    AIALTVDTPR LGRRESDIKN RFTLPPNLTL KNFEGLDLGK MDEANDSGLA 200
    SYVAGQIDRT LSWKDIQWLQ TITNMPILVK GVLTGEDARI AIQAGAAGII 250
    VSNHGARQLD YVPATISALE EVVKATQGRV PVFLDGGVRR GTDVFKALAL 300
    GASGIFIGRP VVFALAAEGE AGVKKVLQML RDEFELTMAL SGCRSLSEIT 350
    RNHIVTEWDT PRHLPRL 367
    Length:367
    Mass (Da):40,307
    Last modified:October 1, 2000 - v1
    Checksum:iC0B0F9B083F1B6E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028617, AB022220 Genomic DNA. Translation: BAB01333.1.
    CP002686 Genomic DNA. Translation: AEE75515.1.
    CP002686 Genomic DNA. Translation: AEE75517.1.
    AY058095 mRNA. Translation: AAL24203.1.
    AY136402 mRNA. Translation: AAM97068.1.
    BT002129 mRNA. Translation: AAN72140.1.
    AK221716 mRNA. Translation: BAD95441.1.
    RefSeqiNP_001189892.1. NM_001202963.1. [Q9LRS0-1]
    NP_188059.1. NM_112301.2. [Q9LRS0-1]
    UniGeneiAt.20363.
    At.67927.

    Genome annotation databases

    EnsemblPlantsiAT3G14415.1; AT3G14415.1; AT3G14415. [Q9LRS0-1]
    AT3G14415.3; AT3G14415.3; AT3G14415. [Q9LRS0-1]
    GeneIDi820664.
    KEGGiath:AT3G14415.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028617 , AB022220 Genomic DNA. Translation: BAB01333.1 .
    CP002686 Genomic DNA. Translation: AEE75515.1 .
    CP002686 Genomic DNA. Translation: AEE75517.1 .
    AY058095 mRNA. Translation: AAL24203.1 .
    AY136402 mRNA. Translation: AAM97068.1 .
    BT002129 mRNA. Translation: AAN72140.1 .
    AK221716 mRNA. Translation: BAD95441.1 .
    RefSeqi NP_001189892.1. NM_001202963.1. [Q9LRS0-1 ]
    NP_188059.1. NM_112301.2. [Q9LRS0-1 ]
    UniGenei At.20363.
    At.67927.

    3D structure databases

    ProteinModelPortali Q9LRS0.
    SMRi Q9LRS0. Positions 1-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5998. 5 interactions.
    IntActi Q9LRS0. 1 interaction.
    STRINGi 3702.AT3G14415.1-P.

    Proteomic databases

    PaxDbi Q9LRS0.
    PRIDEi Q9LRS0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G14415.1 ; AT3G14415.1 ; AT3G14415 . [Q9LRS0-1 ]
    AT3G14415.3 ; AT3G14415.3 ; AT3G14415 . [Q9LRS0-1 ]
    GeneIDi 820664.
    KEGGi ath:AT3G14415.

    Organism-specific databases

    TAIRi AT3G14415.

    Phylogenomic databases

    eggNOGi COG1304.
    HOGENOMi HOG000217463.
    InParanoidi Q9LRS0.
    KOi K11517.
    PhylomeDBi Q9LRS0.

    Enzyme and pathway databases

    UniPathwayi UPA00951 ; UER00912 .
    BioCyci ARA:AT3G14415-MONOMER.
    ARA:GQT-740-MONOMER.
    ARA:GQT-741-MONOMER.

    Gene expression databases

    Genevestigatori Q9LRS0.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms."
      Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O.
      Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 240-257 AND 280-289, SUBCELLULAR LOCATION.
    6. "Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana."
      Fukao Y., Hayashi M., Nishimura M.
      Plant Cell Physiol. 43:689-696(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
      Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
      J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiGLO2_ARATH
    AccessioniPrimary (citable) accession number: Q9LRS0
    Secondary accession number(s): Q56XF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3