Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9LRS0 (GOX1_ARATH)

Last modified February 9, 2010. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable peroxisomal (S)-2-hydroxy-acid oxidase 1
    EC=1.1.3.15
Alternative name(s):
    Glycolate oxidase 1
      Short name=GOX 1
    Short chain alpha-hydroxy acid oxidase 1

Gene names

Ordered Locus Names:	At3g14415
ORF Names:	MLN21.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	(S)-2-hydroxy acid + O₂ = 2-oxo acid + H₂O₂.
Cofactor	FMN By similarity.
Pathway	Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.
Subunit structure	Homotetramer or homooctamer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

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Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC FMN binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

Probable peroxisomal (S)-2-hydroxy-acid oxidase 1

PRO_0000206323

Regions

Domain

1 – 359

359

FMN hydroxy acid dehydrogenase

Nucleotide binding

285 – 309

FMN By similarity

Motif

365 – 367

Microbody targeting signal Potential

Sites

Active site

254

Proton acceptor By similarity

Binding site

Substrate Potential

Binding site

106

FMN By similarity

Binding site

127

FMN By similarity

Binding site

129

Substrate By similarity

Binding site

155

FMN By similarity

Binding site

164

Substrate By similarity

Binding site

230

FMN By similarity

Binding site

257

Substrate Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9LRS0-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C0B0F9B083F1B6E6
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FASTA

367

40,307

        10         20         30         40         50         60 
MEITNVTEYD AIAKAKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR ILIDVNKIDM 

        70         80         90        100        110        120 
ATTVLGFKIS MPIMVAPTAF QKMAHPDGEY ATARAASAAG TIMTLSSWAT SSVEEVASTG 

       130        140        150        160        170        180 
PGIRFFQLYV YKNRKVVEQL VRRAEKAGFK AIALTVDTPR LGRRESDIKN RFTLPPNLTL 

       190        200        210        220        230        240 
KNFEGLDLGK MDEANDSGLA SYVAGQIDRT LSWKDIQWLQ TITNMPILVK GVLTGEDARI 

       250        260        270        280        290        300 
AIQAGAAGII VSNHGARQLD YVPATISALE EVVKATQGRV PVFLDGGVRR GTDVFKALAL 

       310        320        330        340        350        360 
GASGIFIGRP VVFALAAEGE AGVKKVLQML RDEFELTMAL SGCRSLSEIT RNHIVTEWDT 


PRHLPRL

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References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028617, AB022220 Genomic DNA. Translation: BAB01333.1. AY058095 mRNA. Translation: AAL24203.1. AY136402 mRNA. Translation: AAM97068.1. BT002129 mRNA. Translation: AAN72140.1.
IPI	IPI00518426.
RefSeq	NP_188059.1.
UniGene	At.20363 At.67927 Rra.4802
3D structure databases
SMR	Q9LRS0. Positions 1-359.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LRS0.
Proteomic databases
PRIDE	Q9LRS0.
ProMEX	Q9LRS0.
Genome annotation databases
GeneID	820664.
GenomeReviews	Gene locus AT3G14415 in contig BA000014_GR.
KEGG	ath:AT3G14415.
NMPDR	fig\|3702.1.peg.13578.
Organism-specific databases
TAIR	At3g14415.
Phylogenomic databases
HOGENOM	HBG517781.
InParanoid	Q9LRS0.
OMA	MITCREN.
PhylomeDB	Q9LRS0.
Enzyme and pathway databases
BRENDA	1.1.3.15. 302.
Gene expression databases
ArrayExpress	Q9LRS0.
Genevestigator	Q9LRS0.
GermOnline	AT3G14415. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GOX1_ARATH

Accession

Primary (citable) accession number: Q9LRS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents