Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 - Arabidopsis thaliana (Mouse-ear cress)

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Q9LRS0 (GLO2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO2
EC=1.1.3.15

Alternative name(s):
Glycolate oxidase 1
Short name=AtGLO2
Short name=GOX 1
Short chain alpha-hydroxy acid oxidase GLO2

Gene names

Name:	GLO2
Synonyms:	GOX1
Ordered Locus Names:	At3g14415
ORF Names:	MLN21.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-2-hydroxy acid + O₂ = 2-oxo acid + H₂O₂.
Cofactor	FMN By similarity.
Pathway	Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.
Subunit structure	Homotetramer or homooctamer By similarity.
Subcellular location	Peroxisome Ref.5 Ref.6.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay Ref.5. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC FMN binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LRS0-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

Peroxisomal (S)-2-hydroxy-acid oxidase GLO2

PRO_0000206323

Regions

Domain

1 – 359

359

FMN hydroxy acid dehydrogenase

Nucleotide binding

285 – 309

FMN By similarity

Motif

365 – 367

Microbody targeting signal Potential

Sites

Active site

254

Proton acceptor By similarity

Binding site

Substrate Potential

Binding site

106

FMN By similarity

Binding site

127

FMN By similarity

Binding site

129

Substrate By similarity

Binding site

155

FMN By similarity

Binding site

164

Substrate By similarity

Binding site

230

FMN By similarity

Binding site

257

Substrate Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C0B0F9B083F1B6E6
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FASTA

367

40,307

        10         20         30         40         50         60 
MEITNVTEYD AIAKAKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR ILIDVNKIDM 

        70         80         90        100        110        120 
ATTVLGFKIS MPIMVAPTAF QKMAHPDGEY ATARAASAAG TIMTLSSWAT SSVEEVASTG 

       130        140        150        160        170        180 
PGIRFFQLYV YKNRKVVEQL VRRAEKAGFK AIALTVDTPR LGRRESDIKN RFTLPPNLTL 

       190        200        210        220        230        240 
KNFEGLDLGK MDEANDSGLA SYVAGQIDRT LSWKDIQWLQ TITNMPILVK GVLTGEDARI 

       250        260        270        280        290        300 
AIQAGAAGII VSNHGARQLD YVPATISALE EVVKATQGRV PVFLDGGVRR GTDVFKALAL 

       310        320        330        340        350        360 
GASGIFIGRP VVFALAAEGE AGVKKVLQML RDEFELTMAL SGCRSLSEIT RNHIVTEWDT 


PRHLPRL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms." Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O. Plant Cell 19:3170-3193(2007) [PubMed: 17951448] [Abstract] Cited for: PROTEIN SEQUENCE OF 240-257 AND 280-289, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana." Fukao Y., Hayashi M., Nishimura M. Plant Cell Physiol. 43:689-696(2002) [PubMed: 12154131] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice." Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X. J. Exp. Bot. 60:1799-1809(2009) [PubMed: 19264754] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028617, AB022220 Genomic DNA. Translation: BAB01333.1. CP002686 Genomic DNA. Translation: AEE75515.1. CP002686 Genomic DNA. Translation: AEE75517.1. AY058095 mRNA. Translation: AAL24203.1. AY136402 mRNA. Translation: AAM97068.1. BT002129 mRNA. Translation: AAN72140.1. AK221716 mRNA. Translation: BAD95441.1.
IPI	IPI00518426.
RefSeq	NP_001189892.1. NM_001202963.1. NP_188059.1. NM_112301.2.
UniGene	At.20363. At.67927.
3D structure databases
ProteinModelPortal	Q9LRS0.
SMR	Q9LRS0. Positions 1-359.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LRS0. 1 interaction.
STRING	Q9LRS0.
Proteomic databases
PRIDE	Q9LRS0.
ProMEX	Q9LRS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G14415.1; AT3G14415.1; AT3G14415. AT3G14415.3; AT3G14415.3; AT3G14415.
GeneID	820664.
GenomeReviews	Gene locus AT3G14415 in contig BA000014_GR.
KEGG	ath:AT3G14415.
NMPDR	fig\|3702.1.peg.13578.
Organism-specific databases
TAIR	At3g14415.
Phylogenomic databases
GeneTree	EPGT00050000011059.
HOGENOM	HBG517781.
InParanoid	Q9LRS0.
OMA	SEITRNH.
PhylomeDB	Q9LRS0.
ProtClustDB	PLN02493.
Gene expression databases
ArrayExpress	Q9LRS0.
Genevestigator	Q9LRS0.
GermOnline	AT3G14415. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K11517.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO2_ARATH

Accession

Primary (citable) accession number: Q9LRS0
Secondary accession number(s): Q56XF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents