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Q9LRR9

- GLO1_ARATH

UniProt

Q9LRR9 - GLO1_ARATH

Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO1

Gene

GLO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241SubstratePROSITE-ProRule annotation
    Binding sitei106 – 1061FMNPROSITE-ProRule annotation
    Binding sitei127 – 1271FMNPROSITE-ProRule annotation
    Binding sitei129 – 1291SubstratePROSITE-ProRule annotation
    Binding sitei155 – 1551FMNPROSITE-ProRule annotation
    Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
    Binding sitei230 – 2301FMNPROSITE-ProRule annotation
    Active sitei254 – 2541Proton acceptorPROSITE-ProRule annotation
    Binding sitei257 – 2571SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi285 – 30925FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. glycolate oxidase activity Source: TAIR
    3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response to bacterium Source: TAIR
    2. hydrogen peroxide biosynthetic process Source: TAIR
    3. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolate pathway, Photorespiration

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciARA:AT3G14420-MONOMER.
    ARA:GQT-2237-MONOMER.
    ARA:GQT-2238-MONOMER.
    ARA:GQT-2239-MONOMER.
    ARA:GQT-2240-MONOMER.
    ARA:GQT-2241-MONOMER.
    MetaCyc:AT3G14420-MONOMER.
    UniPathwayiUPA00951; UER00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (EC:1.1.3.15)
    Alternative name(s):
    Glycolate oxidase 1
    Short name:
    AtGLO1
    Short name:
    GOX 1
    Short chain alpha-hydroxy acid oxidase GLO1
    Gene namesi
    Name:GLO1
    Ordered Locus Names:At3g14420
    ORF Names:MAO2.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G14420.

    Subcellular locationi

    Peroxisome 2 Publications

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. chloroplast stroma Source: TAIR
    4. cytosolic ribosome Source: TAIR
    5. membrane Source: TAIR
    6. nucleus Source: TAIR
    7. peroxisome Source: TAIR
    8. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Peroxisomal (S)-2-hydroxy-acid oxidase GLO1PRO_0000206324Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9LRR9.
    PRIDEiQ9LRR9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LRR9.

    Interactioni

    Subunit structurei

    Homotetramer or homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi5999. 4 interactions.
    IntActiQ9LRR9. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LRR9.
    SMRiQ9LRR9. Positions 1-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 359359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi365 – 3673Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    HOGENOMiHOG000217463.
    InParanoidiQ9LRR9.
    KOiK11517.
    OMAiISMPICA.
    PhylomeDBiQ9LRR9.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9LRR9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEITNVTEYD AIAKQKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR    50
    ILIDVSKIDM TTTVLGFKIS MPIMVAPTAM QKMAHPDGEY ATARAASAAG 100
    TIMTLSSWAT SSVEEVASTG PGIRFFQLYV YKNRNVVEQL VRRAERAGFK 150
    AIALTVDTPR LGRRESDIKN RFTLPPNLTL KNFEGLDLGK MDEANDSGLA 200
    SYVAGQIDRT LSWKDVQWLQ TITKLPILVK GVLTGEDARI AIQAGAAGII 250
    VSNHGARQLD YVPATISALE EVVKATQGRI PVFLDGGVRR GTDVFKALAL 300
    GASGIFIGRP VVFSLAAEGE AGVRKVLQML RDEFELTMAL SGCRSLKEIS 350
    RNHITTEWDT PRPSARL 367
    Length:367
    Mass (Da):40,341
    Last modified:October 1, 2000 - v1
    Checksum:iB107AD7AC983A04C
    GO
    Isoform 2 (identifier: Q9LRR9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-19: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:348
    Mass (Da):38,167
    Checksum:i8A5904F8787FC515
    GO

    Sequence cautioni

    The sequence AAL38298.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551V → A in AAL16258. (PubMed:14593172)Curated
    Sequence conflicti363 – 3631P → L in AAL38298. (PubMed:14593172)Curated
    Sequence conflicti363 – 3631P → L in AAM10128. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1919Missing in isoform 2. 1 PublicationVSP_040387Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028617 Genomic DNA. Translation: BAB01334.1.
    CP002686 Genomic DNA. Translation: AEE75518.1.
    CP002686 Genomic DNA. Translation: AEE75520.1.
    CP002686 Genomic DNA. Translation: AEE75521.1.
    AY053412 mRNA. Translation: AAK96642.1.
    AF428396 mRNA. Translation: AAL16164.1.
    AF428328 mRNA. Translation: AAL16258.1.
    AY065122 mRNA. Translation: AAL38298.1. Different initiation.
    AY074830 mRNA. Translation: AAL69528.1.
    AY081566 mRNA. Translation: AAM10128.1.
    AK317539 mRNA. Translation: BAH20203.1.
    RefSeqiNP_001030694.1. NM_001035617.1. [Q9LRR9-2]
    NP_188060.1. NM_112302.3. [Q9LRR9-1]
    NP_850584.1. NM_180253.1. [Q9LRR9-1]
    UniGeneiAt.21768.
    At.67007.
    At.71586.

    Genome annotation databases

    EnsemblPlantsiAT3G14420.1; AT3G14420.1; AT3G14420. [Q9LRR9-1]
    AT3G14420.2; AT3G14420.2; AT3G14420. [Q9LRR9-1]
    GeneIDi820665.
    KEGGiath:AT3G14420.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028617 Genomic DNA. Translation: BAB01334.1 .
    CP002686 Genomic DNA. Translation: AEE75518.1 .
    CP002686 Genomic DNA. Translation: AEE75520.1 .
    CP002686 Genomic DNA. Translation: AEE75521.1 .
    AY053412 mRNA. Translation: AAK96642.1 .
    AF428396 mRNA. Translation: AAL16164.1 .
    AF428328 mRNA. Translation: AAL16258.1 .
    AY065122 mRNA. Translation: AAL38298.1 . Different initiation.
    AY074830 mRNA. Translation: AAL69528.1 .
    AY081566 mRNA. Translation: AAM10128.1 .
    AK317539 mRNA. Translation: BAH20203.1 .
    RefSeqi NP_001030694.1. NM_001035617.1. [Q9LRR9-2 ]
    NP_188060.1. NM_112302.3. [Q9LRR9-1 ]
    NP_850584.1. NM_180253.1. [Q9LRR9-1 ]
    UniGenei At.21768.
    At.67007.
    At.71586.

    3D structure databases

    ProteinModelPortali Q9LRR9.
    SMRi Q9LRR9. Positions 1-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5999. 4 interactions.
    IntActi Q9LRR9. 3 interactions.

    Proteomic databases

    PaxDbi Q9LRR9.
    PRIDEi Q9LRR9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G14420.1 ; AT3G14420.1 ; AT3G14420 . [Q9LRR9-1 ]
    AT3G14420.2 ; AT3G14420.2 ; AT3G14420 . [Q9LRR9-1 ]
    GeneIDi 820665.
    KEGGi ath:AT3G14420.

    Organism-specific databases

    TAIRi AT3G14420.

    Phylogenomic databases

    eggNOGi COG1304.
    HOGENOMi HOG000217463.
    InParanoidi Q9LRR9.
    KOi K11517.
    OMAi ISMPICA.
    PhylomeDBi Q9LRR9.

    Enzyme and pathway databases

    UniPathwayi UPA00951 ; UER00912 .
    BioCyci ARA:AT3G14420-MONOMER.
    ARA:GQT-2237-MONOMER.
    ARA:GQT-2238-MONOMER.
    ARA:GQT-2239-MONOMER.
    ARA:GQT-2240-MONOMER.
    ARA:GQT-2241-MONOMER.
    MetaCyc:AT3G14420-MONOMER.

    Gene expression databases

    Genevestigatori Q9LRR9.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms."
      Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O.
      Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 240-257, SUBCELLULAR LOCATION.
    6. "Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana."
      Fukao Y., Hayashi M., Nishimura M.
      Plant Cell Physiol. 43:689-696(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
      Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
      J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiGLO1_ARATH
    AccessioniPrimary (citable) accession number: Q9LRR9
    Secondary accession number(s): B9DHI6, Q8VZA4, Q944K6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3