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Q9LRR9

- GLO1_ARATH

UniProt

Q9LRR9 - GLO1_ARATH

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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO1

Gene

GLO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMN.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241SubstratePROSITE-ProRule annotation
Binding sitei106 – 1061FMNPROSITE-ProRule annotation
Binding sitei127 – 1271FMNPROSITE-ProRule annotation
Binding sitei129 – 1291SubstratePROSITE-ProRule annotation
Binding sitei155 – 1551FMNPROSITE-ProRule annotation
Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
Binding sitei230 – 2301FMNPROSITE-ProRule annotation
Active sitei254 – 2541Proton acceptorPROSITE-ProRule annotation
Binding sitei257 – 2571SubstratePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi285 – 30925FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. glycolate oxidase activity Source: TAIR
  3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
  4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
  5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response to bacterium Source: TAIR
  2. hydrogen peroxide biosynthetic process Source: TAIR
  3. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
  4. response to cytokinin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14420-MONOMER.
ARA:GQT-2237-MONOMER.
ARA:GQT-2238-MONOMER.
ARA:GQT-2239-MONOMER.
ARA:GQT-2240-MONOMER.
ARA:GQT-2241-MONOMER.
MetaCyc:AT3G14420-MONOMER.
UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 1
Short name:
AtGLO1
Short name:
GOX 1
Short chain alpha-hydroxy acid oxidase GLO1
Gene namesi
Name:GLO1
Ordered Locus Names:At3g14420
ORF Names:MAO2.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G14420.

Subcellular locationi

Peroxisome 2 Publications

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. cytosolic ribosome Source: TAIR
  5. membrane Source: TAIR
  6. nucleus Source: TAIR
  7. peroxisome Source: TAIR
  8. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Peroxisomal (S)-2-hydroxy-acid oxidase GLO1PRO_0000206324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LRR9.
PRIDEiQ9LRR9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LRR9. baseline.
GenevestigatoriQ9LRR9.

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

BioGridi5999. 4 interactions.
IntActiQ9LRR9. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9LRR9.
SMRiQ9LRR9. Positions 1-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 359359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi365 – 3673Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1304.
HOGENOMiHOG000217463.
InParanoidiQ9LRR9.
KOiK11517.
OMAiISMPICA.
PhylomeDBiQ9LRR9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LRR9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEITNVTEYD AIAKQKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR
60 70 80 90 100
ILIDVSKIDM TTTVLGFKIS MPIMVAPTAM QKMAHPDGEY ATARAASAAG
110 120 130 140 150
TIMTLSSWAT SSVEEVASTG PGIRFFQLYV YKNRNVVEQL VRRAERAGFK
160 170 180 190 200
AIALTVDTPR LGRRESDIKN RFTLPPNLTL KNFEGLDLGK MDEANDSGLA
210 220 230 240 250
SYVAGQIDRT LSWKDVQWLQ TITKLPILVK GVLTGEDARI AIQAGAAGII
260 270 280 290 300
VSNHGARQLD YVPATISALE EVVKATQGRI PVFLDGGVRR GTDVFKALAL
310 320 330 340 350
GASGIFIGRP VVFSLAAEGE AGVRKVLQML RDEFELTMAL SGCRSLKEIS
360
RNHITTEWDT PRPSARL
Length:367
Mass (Da):40,341
Last modified:October 1, 2000 - v1
Checksum:iB107AD7AC983A04C
GO
Isoform 2 (identifier: Q9LRR9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Note: No experimental confirmation available.

Show »
Length:348
Mass (Da):38,167
Checksum:i8A5904F8787FC515
GO

Sequence cautioni

The sequence AAL38298.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551V → A in AAL16258. (PubMed:14593172)Curated
Sequence conflicti363 – 3631P → L in AAL38298. (PubMed:14593172)Curated
Sequence conflicti363 – 3631P → L in AAM10128. (PubMed:14593172)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform 2. 1 PublicationVSP_040387Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028617 Genomic DNA. Translation: BAB01334.1.
CP002686 Genomic DNA. Translation: AEE75518.1.
CP002686 Genomic DNA. Translation: AEE75520.1.
CP002686 Genomic DNA. Translation: AEE75521.1.
AY053412 mRNA. Translation: AAK96642.1.
AF428396 mRNA. Translation: AAL16164.1.
AF428328 mRNA. Translation: AAL16258.1.
AY065122 mRNA. Translation: AAL38298.1. Different initiation.
AY074830 mRNA. Translation: AAL69528.1.
AY081566 mRNA. Translation: AAM10128.1.
AK317539 mRNA. Translation: BAH20203.1.
RefSeqiNP_001030694.1. NM_001035617.1. [Q9LRR9-2]
NP_188060.1. NM_112302.3. [Q9LRR9-1]
NP_850584.1. NM_180253.1. [Q9LRR9-1]
UniGeneiAt.21768.
At.67007.
At.71586.

Genome annotation databases

EnsemblPlantsiAT3G14420.1; AT3G14420.1; AT3G14420. [Q9LRR9-1]
AT3G14420.2; AT3G14420.2; AT3G14420. [Q9LRR9-1]
GeneIDi820665.
KEGGiath:AT3G14420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028617 Genomic DNA. Translation: BAB01334.1 .
CP002686 Genomic DNA. Translation: AEE75518.1 .
CP002686 Genomic DNA. Translation: AEE75520.1 .
CP002686 Genomic DNA. Translation: AEE75521.1 .
AY053412 mRNA. Translation: AAK96642.1 .
AF428396 mRNA. Translation: AAL16164.1 .
AF428328 mRNA. Translation: AAL16258.1 .
AY065122 mRNA. Translation: AAL38298.1 . Different initiation.
AY074830 mRNA. Translation: AAL69528.1 .
AY081566 mRNA. Translation: AAM10128.1 .
AK317539 mRNA. Translation: BAH20203.1 .
RefSeqi NP_001030694.1. NM_001035617.1. [Q9LRR9-2 ]
NP_188060.1. NM_112302.3. [Q9LRR9-1 ]
NP_850584.1. NM_180253.1. [Q9LRR9-1 ]
UniGenei At.21768.
At.67007.
At.71586.

3D structure databases

ProteinModelPortali Q9LRR9.
SMRi Q9LRR9. Positions 1-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5999. 4 interactions.
IntActi Q9LRR9. 3 interactions.

Proteomic databases

PaxDbi Q9LRR9.
PRIDEi Q9LRR9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G14420.1 ; AT3G14420.1 ; AT3G14420 . [Q9LRR9-1 ]
AT3G14420.2 ; AT3G14420.2 ; AT3G14420 . [Q9LRR9-1 ]
GeneIDi 820665.
KEGGi ath:AT3G14420.

Organism-specific databases

TAIRi AT3G14420.

Phylogenomic databases

eggNOGi COG1304.
HOGENOMi HOG000217463.
InParanoidi Q9LRR9.
KOi K11517.
OMAi ISMPICA.
PhylomeDBi Q9LRR9.

Enzyme and pathway databases

UniPathwayi UPA00951 ; UER00912 .
BioCyci ARA:AT3G14420-MONOMER.
ARA:GQT-2237-MONOMER.
ARA:GQT-2238-MONOMER.
ARA:GQT-2239-MONOMER.
ARA:GQT-2240-MONOMER.
ARA:GQT-2241-MONOMER.
MetaCyc:AT3G14420-MONOMER.

Gene expression databases

ExpressionAtlasi Q9LRR9. baseline.
Genevestigatori Q9LRR9.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view ]
Pfami PF01070. FMN_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms."
    Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O.
    Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 240-257, SUBCELLULAR LOCATION.
  6. "Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana."
    Fukao Y., Hayashi M., Nishimura M.
    Plant Cell Physiol. 43:689-696(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
    Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
    J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiGLO1_ARATH
AccessioniPrimary (citable) accession number: Q9LRR9
Secondary accession number(s): B9DHI6, Q8VZA4, Q944K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3