Q9LRR9 (GLO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO1
EC=1.1.3.15

Alternative name(s):
Glycolate oxidase 1
Short name=AtGLO1
Short name=GOX 1
Short chain alpha-hydroxy acid oxidase GLO1

Gene names

Name:	GLO1
Ordered Locus Names:	At3g14420
ORF Names:	MAO2.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.
Cofactor	FMN By similarity.
Pathway	Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.
Subunit structure	Homotetramer or homooctamer By similarity.
Subcellular location	Peroxisome Ref.5 Ref.6.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.
Sequence caution	The sequence AAL38298.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from mutant phenotype PubMed 22286136. Source: TAIR hydrogen peroxide biosynthetic process Inferred from mutant phenotype PubMed 22286136. Source: TAIR oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 18633119. Source: TAIR cytosolic ribosome Inferred from direct assay PubMed 15821981. Source: TAIR membrane Inferred from direct assay PubMed 17432890. Source: TAIR nucleus Inferred from direct assay PubMed 14617066. Source: TAIR peroxisome Inferred from direct assay Ref.5. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro glycolate oxidase activity Inferred from mutant phenotype PubMed 18685041. Source: TAIR long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Inferred from electronic annotation. Source: EC medium-chain-(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC very-long-chain-(S)-2-hydroxy-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LRR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LRR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Peroxisomal (S)-2-hydroxy-acid oxidase GLO1		PRO_0000206324
Regions
	Domain	1 – 359	359	FMN hydroxy acid dehydrogenase
	Nucleotide binding	285 – 309	25	FMN By similarity
	Motif	365 – 367	3	Microbody targeting signal Potential
Sites
	Active site	254	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	230	1	FMN By similarity
	Binding site	257	1	Substrate Potential
Natural variations
	Alternative sequence	1 – 19	19	Missing in isoform 2.		VSP_040387
Experimental info
	Sequence conflict	55	1	V → A in AAL16258. Ref.3
	Sequence conflict	363	1	P → L in AAL38298. Ref.3
	Sequence conflict	363	1	P → L in AAM10128. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B107AD7AC983A04C	FASTA	367	40,341
	10 20 30 40 50 60 MEITNVTEYD AIAKQKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR ILIDVSKIDM 70 80 90 100 110 120 TTTVLGFKIS MPIMVAPTAM QKMAHPDGEY ATARAASAAG TIMTLSSWAT SSVEEVASTG 130 140 150 160 170 180 PGIRFFQLYV YKNRNVVEQL VRRAERAGFK AIALTVDTPR LGRRESDIKN RFTLPPNLTL 190 200 210 220 230 240 KNFEGLDLGK MDEANDSGLA SYVAGQIDRT LSWKDVQWLQ TITKLPILVK GVLTGEDARI 250 260 270 280 290 300 AIQAGAAGII VSNHGARQLD YVPATISALE EVVKATQGRI PVFLDGGVRR GTDVFKALAL 310 320 330 340 350 360 GASGIFIGRP VVFSLAAEGE AGVRKVLQML RDEFELTMAL SGCRSLKEIS RNHITTEWDT PRPSARL
	Isoform 2 [UniParc]. Checksum: 8A5904F8787FC515	FASTA	348	38,167
	10 20 30 40 50 60 MVYDYYASGA EDQWTLQENR NAFARILFRP RILIDVSKID MTTTVLGFKI SMPIMVAPTA 70 80 90 100 110 120 MQKMAHPDGE YATARAASAA GTIMTLSSWA TSSVEEVAST GPGIRFFQLY VYKNRNVVEQ 130 140 150 160 170 180 LVRRAERAGF KAIALTVDTP RLGRRESDIK NRFTLPPNLT LKNFEGLDLG KMDEANDSGL 190 200 210 220 230 240 ASYVAGQIDR TLSWKDVQWL QTITKLPILV KGVLTGEDAR IAIQAGAAGI IVSNHGARQL 250 260 270 280 290 300 DYVPATISAL EEVVKATQGR IPVFLDGGVR RGTDVFKALA LGASGIFIGR PVVFSLAAEG 310 320 330 340 EAGVRKVLQM LRDEFELTMA LSGCRSLKEI SRNHITTEWD TPRPSARL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms." Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O. Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 240-257, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana." Fukao Y., Hayashi M., Nishimura M. Plant Cell Physiol. 43:689-696(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice." Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X. J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028617 Genomic DNA. Translation: BAB01334.1. CP002686 Genomic DNA. Translation: AEE75518.1. CP002686 Genomic DNA. Translation: AEE75520.1. CP002686 Genomic DNA. Translation: AEE75521.1. AY053412 mRNA. Translation: AAK96642.1. AF428396 mRNA. Translation: AAL16164.1. AF428328 mRNA. Translation: AAL16258.1. AY065122 mRNA. Translation: AAL38298.1. Different initiation. AY074830 mRNA. Translation: AAL69528.1. AY081566 mRNA. Translation: AAM10128.1. AK317539 mRNA. Translation: BAH20203.1.
IPI	IPI00528534. IPI00656816.
RefSeq	NP_001030694.1. NM_001035617.1. NP_188060.1. NM_112302.3. NP_850584.1. NM_180253.1.
UniGene	At.21768. At.67007. At.71586.
3D structure databases
ProteinModelPortal	Q9LRR9.
SMR	Q9LRR9. Positions 1-359.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LRR9. 2 interactions.
Proteomic databases
PaxDb	Q9LRR9.
PRIDE	Q9LRR9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G14420.1; AT3G14420.1; AT3G14420. AT3G14420.2; AT3G14420.2; AT3G14420.
GeneID	820665.
KEGG	ath:AT3G14420.
Organism-specific databases
TAIR	At3g14420.
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HOG000217463.
InParanoid	Q9LRR9.
KO	K11517.
OMA	ESPTMST.
PhylomeDB	Q9LRR9.
ProtClustDB	PLN02493.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G14420-MONOMER.
UniPathway	UPA00951; UER00912.
Gene expression databases
ArrayExpress	Q9LRR9.
Genevestigator	Q9LRR9.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO1_ARATH

Accession

Primary (citable) accession number: Q9LRR9
Secondary accession number(s): B9DHI6, Q8VZA4, Q944K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program