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Protein

(S)-2-hydroxy-acid oxidase GLO1

Gene

GLO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), (S)-2-hydroxy-acid oxidase GLO2 (GLO2), (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24SubstratePROSITE-ProRule annotation1
Binding sitei106FMNPROSITE-ProRule annotation1
Binding sitei127FMNPROSITE-ProRule annotation1
Binding sitei129SubstratePROSITE-ProRule annotation1
Binding sitei155FMNPROSITE-ProRule annotation1
Binding sitei164SubstratePROSITE-ProRule annotation1
Binding sitei230FMNPROSITE-ProRule annotation1
Active sitei254Proton acceptorPROSITE-ProRule annotation1
Binding sitei257SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 309FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolate pathway, Photorespiration
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14420-MONOMER.
MetaCyc:AT3G14420-MONOMER.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-390918. Peroxisomal lipid metabolism.
UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-hydroxy-acid oxidase GLO1 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 1
Short name:
AtGLO1
Short name:
GOX 1
Short chain alpha-hydroxy acid oxidase GLO1
Gene namesi
Name:GLO1
Ordered Locus Names:At3g14420
ORF Names:MAO2.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G14420.
TAIRilocus:2091642. AT3G14420.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002063241 – 367(S)-2-hydroxy-acid oxidase GLO1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LRR9.
PRIDEiQ9LRR9.

PTM databases

iPTMnetiQ9LRR9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LRR9. baseline and differential.
GenevisibleiQ9LRR9. AT.

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

BioGridi5999. 4 interactors.
IntActiQ9LRR9. 3 interactors.
STRINGi3702.AT3G14420.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LRR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST359

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
HOGENOMiHOG000217463.
InParanoidiQ9LRR9.
KOiK11517.
OMAiMQLYIYK.
PhylomeDBiQ9LRR9.

Family and domain databases

CDDicd02809. alpha_hydroxyacid_oxid_FMN. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
PfamiView protein in Pfam
PF01070. FMN_dh. 1 hit.
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiView protein in PROSITE
PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LRR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEITNVTEYD AIAKQKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR
60 70 80 90 100
ILIDVSKIDM TTTVLGFKIS MPIMVAPTAM QKMAHPDGEY ATARAASAAG
110 120 130 140 150
TIMTLSSWAT SSVEEVASTG PGIRFFQLYV YKNRNVVEQL VRRAERAGFK
160 170 180 190 200
AIALTVDTPR LGRRESDIKN RFTLPPNLTL KNFEGLDLGK MDEANDSGLA
210 220 230 240 250
SYVAGQIDRT LSWKDVQWLQ TITKLPILVK GVLTGEDARI AIQAGAAGII
260 270 280 290 300
VSNHGARQLD YVPATISALE EVVKATQGRI PVFLDGGVRR GTDVFKALAL
310 320 330 340 350
GASGIFIGRP VVFSLAAEGE AGVRKVLQML RDEFELTMAL SGCRSLKEIS
360
RNHITTEWDT PRPSARL
Length:367
Mass (Da):40,341
Last modified:October 1, 2000 - v1
Checksum:iB107AD7AC983A04C
GO
Isoform 2 (identifier: Q9LRR9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Note: No experimental confirmation available.
Show »
Length:348
Mass (Da):38,167
Checksum:i8A5904F8787FC515
GO

Sequence cautioni

Q9LRR9: The sequence AAL38298 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55V → A in AAL16258 (PubMed:14593172).Curated1
Sequence conflicti363P → L in AAL38298 (PubMed:14593172).Curated1
Sequence conflicti363P → L in AAM10128 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0403871 – 19Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028617 Genomic DNA. Translation: BAB01334.1.
CP002686 Genomic DNA. Translation: AEE75518.1.
CP002686 Genomic DNA. Translation: AEE75520.1.
CP002686 Genomic DNA. Translation: AEE75521.1.
AY053412 mRNA. Translation: AAK96642.1.
AF428396 mRNA. Translation: AAL16164.1.
AF428328 mRNA. Translation: AAL16258.1.
AY065122 mRNA. Translation: AAL38298.1. Different initiation.
AY074830 mRNA. Translation: AAL69528.1.
AY081566 mRNA. Translation: AAM10128.1.
AK317539 mRNA. Translation: BAH20203.1.
RefSeqiNP_001030694.1. NM_001035617.1. [Q9LRR9-2]
NP_188060.1. NM_112302.4. [Q9LRR9-1]
NP_850584.1. NM_180253.2. [Q9LRR9-1]
UniGeneiAt.21768.
At.67007.
At.71586.

Genome annotation databases

EnsemblPlantsiAT3G14420.1; AT3G14420.1; AT3G14420. [Q9LRR9-1]
AT3G14420.2; AT3G14420.2; AT3G14420. [Q9LRR9-1]
AT3G14420.4; AT3G14420.4; AT3G14420. [Q9LRR9-2]
GeneIDi820665.
GrameneiAT3G14420.1; AT3G14420.1; AT3G14420.
AT3G14420.2; AT3G14420.2; AT3G14420.
AT3G14420.4; AT3G14420.4; AT3G14420.
KEGGiath:AT3G14420.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLO1_ARATH
AccessioniPrimary (citable) accession number: Q9LRR9
Secondary accession number(s): B9DHI6, Q8VZA4, Q944K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families