Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9LRR9 (GOX2_ARATH)

Last modified June 16, 2009. Version 66. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable peroxisomal (S)-2-hydroxy-acid oxidase 2
    EC=1.1.3.15
Alternative name(s):
    Glycolate oxidase 2
      Short name=GOX 2
    Short chain alpha-hydroxy acid oxidase 2

Gene names

Ordered Locus Names:	At3g14420
ORF Names:	MAO2.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	(S)-2-hydroxy acid + O₂ = 2-oxo acid + H₂O₂.
Cofactor	FMN By similarity.
Pathway	Photosynthesis; photorespiration; glycine from 2-phosphoglycolic acid: step 2/3.
Subunit structure	Homotetramer or homooctamer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

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Ontologies

Keywords
Biological process	Glycolate pathway Photorespiration
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxidative photosynthetic carbon pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR cytosolic ribosome Inferred from direct assay. Source: TAIR membrane Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay. Source: TAIR
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glycolate oxidase activity Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

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Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LRR9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

Probable peroxisomal (S)-2-hydroxy-acid oxidase 2

PRO_0000206324

Regions

Domain

1 – 359

359

FMN hydroxy acid dehydrogenase

Nucleotide binding

285 – 309

FMN By similarity

Motif

365 – 367

Microbody targeting signal Potential

Sites

Active site

254

Proton acceptor By similarity

Binding site

Substrate Potential

Binding site

106

FMN By similarity

Binding site

127

FMN By similarity

Binding site

129

Substrate By similarity

Binding site

155

FMN By similarity

Binding site

164

Substrate By similarity

Binding site

230

FMN By similarity

Binding site

257

Substrate Potential

Experimental info

Sequence conflict

V → A in AAL16258. Ref.2

Sequence conflict

363

P → L in AAL38298. Ref.2

Sequence conflict

363

P → L in AAM10128. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B107AD7AC983A04C
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FASTA

367

40,341

        10         20         30         40         50         60 
MEITNVTEYD AIAKQKLPKM VYDYYASGAE DQWTLQENRN AFARILFRPR ILIDVSKIDM 

        70         80         90        100        110        120 
TTTVLGFKIS MPIMVAPTAM QKMAHPDGEY ATARAASAAG TIMTLSSWAT SSVEEVASTG 

       130        140        150        160        170        180 
PGIRFFQLYV YKNRNVVEQL VRRAERAGFK AIALTVDTPR LGRRESDIKN RFTLPPNLTL 

       190        200        210        220        230        240 
KNFEGLDLGK MDEANDSGLA SYVAGQIDRT LSWKDVQWLQ TITKLPILVK GVLTGEDARI 

       250        260        270        280        290        300 
AIQAGAAGII VSNHGARQLD YVPATISALE EVVKATQGRI PVFLDGGVRR GTDVFKALAL 

       310        320        330        340        350        360 
GASGIFIGRP VVFSLAAEGE AGVRKVLQML RDEFELTMAL SGCRSLKEIS RNHITTEWDT 


PRPSARL

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References

[1]

"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

[2]

"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.

Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Columbia.

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Cross-references

Sequence databases
	AB028617 Genomic DNA. Translation: BAB01334.1. AY053412 mRNA. Translation: AAK96642.1. AF428396 mRNA. Translation: AAL16164.1. AF428328 mRNA. Translation: AAL16258.1. AY065122 mRNA. Translation: AAL38298.1. Different initiation. AY074830 mRNA. Translation: AAL69528.1. AY081566 mRNA. Translation: AAM10128.1.
IPI	IPI00528534.
RefSeq	NP_188060.1. NP_850584.1.
UniGene	At.21768 At.67007 At.71586
3D structure databases
HSSP	HSSP built from PDB template 1AL8 based on UniProtKB P05414.
SMR	Q9LRR9. Positions 1-359.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LRR9. 1 interaction.
Proteomic databases
PRIDE	Q9LRR9.
ProMEX	Q9LRR9.
Genome annotation databases
GeneID	820665.
GenomeReviews	Gene locus AT3G14420 in contig BA000014_GR.
NMPDR	fig\|3702.1.peg.13579.
Organism-specific databases
TAIR	At3g14420.
Phylogenomic databases
OMA	Q9LRR9. ADITRNH.
Enzyme and pathway databases
BRENDA	1.1.3.15. 302.
Family and domain databases
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR017934. FMN-dep_OHA_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GOX2_ARATH

Accession

Primary (citable) accession number: Q9LRR9
Secondary accession number(s): Q8VZA4, Q944K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents