9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic precursor

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Q9LRR7 (NCED3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic
Short name=AtNCED3
EC=1.13.11.51

Alternative name(s):
Protein SALT TOLERANT 1

Gene names

Name:	NCED3
Synonyms:	STO1
Ordered Locus Names:	At3g14440
ORF Names:	MOA2.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids, in response to water stress. Ref.1 Ref.6
Catalytic activity	A 9-cis-epoxycarotenoid + O₂ = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal. 9-cis-violaxanthin + O₂ = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al. 9'-cis-neoxanthin + O₂ = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
Cofactor	Binds 1 Fe²⁺ ion per subunit By similarity.
Enzyme regulation	Inhibited by abamine and abamineSG. Ref.7 Ref.8
Subcellular location	Plastid › chloroplast stroma. Note: Partially bound to the thylakoid. Ref.5
Tissue specificity	Localized in roots, leaves, stems, empty silique envelopes and seeds. Expressed at the point of organ attachment and the abscission zones in the plant. Ref.5 Ref.6 Ref.9
Induction	By salt or drought stress and pathogen. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10
Disruption phenotype	Plants show enhanced germination on salt and hypersensitivity to desication and LiCl. Ref.1
Miscellaneous	Overexpresion of NCED3 results in increased accumulation of abscisic acid and resistance to water stress.
Sequence similarities	Belongs to the carotenoid oxygenase family.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis Stress response
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hyperosmotic salinity response Inferred from mutant phenotype Ref.6. Source: TAIR response to water deprivation Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay Ref.5. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay Ref.5. Source: TAIR
Molecular function	9-cis-epoxycarotenoid dioxygenase activity Inferred from direct assay Ref.1. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 40

Chloroplast Potential

Chain

41 – 599

559

9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic

PRO_0000285991

Regions

Compositional bias

92 – 96

Poly-Ala

Sites

Metal binding

297

Iron By similarity

Metal binding

346

Iron By similarity

Metal binding

411

Iron By similarity

Metal binding

585

Iron By similarity

Experimental info

Sequence conflict

371

R → L in AAL07104. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9LRR7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 7D513F39945E0CF3
Show »

FASTA

599

65,857

        10         20         30         40         50         60 
MASFTATAAV SGRWLGGNHT QPPLSSSQSS DLSYCSSLPM ASRVTRKLNV SSALHTPPAL 

        70         80         90        100        110        120 
HFPKQSSNSP AIVVKPKAKE SNTKQMNLFQ RAAAAALDAA EGFLVSHEKL HPLPKTADPS 

       130        140        150        160        170        180 
VQIAGNFAPV NEQPVRRNLP VVGKLPDSIK GVYVRNGANP LHEPVTGHHF FDGDGMVHAV 

       190        200        210        220        230        240 
KFEHGSASYA CRFTQTNRFV QERQLGRPVF PKAIGELHGH TGIARLMLFY ARAAAGIVDP 

       250        260        270        280        290        300 
AHGTGVANAG LVYFNGRLLA MSEDDLPYQV QITPNGDLKT VGRFDFDGQL ESTMIAHPKV 

       310        320        330        340        350        360 
DPESGELFAL SYDVVSKPYL KYFRFSPDGT KSPDVEIQLD QPTMMHDFAI TENFVVVPDQ 

       370        380        390        400        410        420 
QVVFKLPEMI RGGSPVVYDK NKVARFGILD KYAEDSSNIK WIDAPDCFCF HLWNAWEEPE 

       430        440        450        460        470        480 
TDEVVVIGSC MTPPDSIFNE SDENLKSVLS EIRLNLKTGE STRRPIISNE DQQVNLEAGM 

       490        500        510        520        530        540 
VNRNMLGRKT KFAYLALAEP WPKVSGFAKV DLTTGEVKKH LYGDNRYGGE PLFLPGEGGE 

       550        560        570        580        590 
EDEGYILCFV HDEKTWKSEL QIVNAVSLEV EATVKLPSRV PYGFHGTFIG ADDLAKQVV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis." Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K. Plant J. 27:325-333(2001) [PubMed: 11532178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION BY DROUGHT STRESS, DISRUPTION PHENOTYPE.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family." Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R. Plant J. 35:44-56(2003) [PubMed: 12834401] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY DROUGHT STRESS.
[6]	"Uncoupling the effects of abscisic acid on plant growth and water relations. Analysis of sto1/nced3, an abscisic acid-deficient but salt stress-tolerant mutant in Arabidopsis." Ruggiero B., Koiwa H., Manabe Y., Quist T.M., Inan G., Saccardo F., Joly R.J., Hasegawa P.M., Bressan R.A., Maggio A. Plant Physiol. 136:3134-3147(2004) [PubMed: 15466233] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY SALT STRESS.
[7]	"A novel inhibitor of 9-cis-epoxycarotenoid dioxygenase in abscisic acid biosynthesis in higher plants." Han S.Y., Kitahata N., Sekimata K., Saito T., Kobayashi M., Nakashima K., Yamaguchi-Shinozaki K., Shinozaki K., Yoshida S., Asami T. Plant Physiol. 135:1574-1582(2004) [PubMed: 15247398] [Abstract] Cited for: ENZYME REGULATION.
[8]	"A 9-cis-epoxycarotenoid dioxygenase inhibitor for use in the elucidation of abscisic acid action mechanisms." Kitahata N., Han S.Y., Noji N., Saito T., Kobayashi M., Nakano T., Kuchitsu K., Shinozaki K., Yoshida S., Matsumoto S., Tsujimoto M., Asami T. Bioorg. Med. Chem. 14:5555-5561(2006) [PubMed: 16682205] [Abstract] Cited for: ENZYME REGULATION.
[9]	"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy." Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A. Plant J. 45:309-319(2006) [PubMed: 16412079] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease." de Torres-Zabala M., Truman W., Bennett M.H., Lafforgue G., Mansfield J.W., Rodriguez Egea P., Bogre L., Grant M. EMBO J. 26:1434-1443(2007) [PubMed: 17304219] [Abstract] Cited for: INDUCTION BY PATHOGEN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026549 Genomic DNA. Translation: BAB70609.1. AB028617 Genomic DNA. Translation: BAB01336.1. CP002686 Genomic DNA. Translation: AEE75526.1. AY056255 mRNA. Translation: AAL07104.1.
IPI	IPI00528622.
RefSeq	NP_188062.1. NM_112304.2.
UniGene	At.26346.
3D structure databases
ProteinModelPortal	Q9LRR7.
SMR	Q9LRR7. Positions 85-597.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LRR7.
Proteomic databases
PRIDE	Q9LRR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G14440.1; AT3G14440.1; AT3G14440.
GeneID	820667.
GenomeReviews	Gene locus AT3G14440 in contig BA000014_GR.
KEGG	ath:AT3G14440.
NMPDR	fig\|3702.1.peg.13583.
Organism-specific databases
TAIR	At3g14440.
Phylogenomic databases
eggNOG	KOG1285.
GeneTree	EPGT00050000007653.
HOGENOM	HBG559594.
InParanoid	Q9LRR7.
OMA	KFAYLAL.
PhylomeDB	Q9LRR7.
ProtClustDB	PLN02258.
Enzyme and pathway databases
BioCyc	ARA:AT3G14440-MONOMER. MetaCyc:AT3G14440-MONOMER.
Gene expression databases
Genevestigator	Q9LRR7.
Family and domain databases
InterPro	IPR004294. Carotenoid_Oase. [Graphical view]
KO	K09840.
PANTHER	PTHR10543. Carotenoid_Oase. 1 hit.
Pfam	PF03055. RPE65. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCED3_ARATH

Accession

Primary (citable) accession number: Q9LRR7
Secondary accession number(s): Q93ZU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents