Q9LRP1 (NPS13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Novel plant SNARE 13
Short name=AtNPSN13

Gene names

Name:	NPSN13
Ordered Locus Names:	At3g17440
ORF Names:	MTO12.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vesicle trafficking protein that functions in the secretory pathway By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the novel plant SNARE family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 15060130PubMed 17317660PubMed 17644812PubMed 22923678. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LRP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Novel plant SNARE 13		PRO_0000213618
Regions
	Topological domain	1 – 217	217	Cytoplasmic Potential
	Transmembrane	218 – 238	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	239 – 269	31	Vesicular Potential
	Domain	146 – 208	63	t-SNARE coiled-coil homology
	Coiled coil	33 – 94	62	Potential
Amino acid modifications
	Modified residue	74	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	188 – 189	2	VN → D in AAM20473. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2238C876A48ACAAC	FASTA	269	30,414
	10 20 30 40 50 60 MASNLPMSPQ LEQIHGEIRD HFRALANGFQ RLDKIKDSTR QSKQLEELTD KMRECKRLVK 70 80 90 100 110 120 EFDRELKDEE ARNSPEVNKQ LNDEKQSMIK ELNSYVALRK TYMSTLGNKK VELFDMGAGV 130 140 150 160 170 180 SGEPTAEENV QVASSMSNQE LVDAGMKRMD ETDQAIERSK QVVEQTLEVG TQTAANLKGQ 190 200 210 220 230 240 TDQMGRVVNH LDTIQFSIKK ASQLVKEIGR QVATDKCIMG FLFLIVCGVV AIIIVKIVNP 250 260 NNKDIRDIPG LAPPAQSRKL LYLRNQDYM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028620 Genomic DNA. Translation: BAB02920.1. CP002686 Genomic DNA. Translation: AEE75953.1. AY099622 mRNA. Translation: AAM20473.1. BT001249 mRNA. Translation: AAN65136.1. AY084645 mRNA. Translation: AAM61208.1.
IPI	IPI00536090.
RefSeq	NP_566578.1. NM_112623.4.
UniGene	At.5879.
3D structure databases
ProteinModelPortal	Q9LRP1.
SMR	Q9LRP1. Positions 149-207.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LRP1. 3 interactions.
Proteomic databases
PaxDb	Q9LRP1.
PRIDE	Q9LRP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G17440.1; AT3G17440.1; AT3G17440.
GeneID	821008.
KEGG	ath:AT3G17440.
Organism-specific databases
GeneFarm	4291. 432.
TAIR	At3g17440.
Phylogenomic databases
eggNOG	NOG314799.
HOGENOM	HOG000240078.
InParanoid	Q9LRP1.
OMA	PQLEQIH.
PhylomeDB	Q9LRP1.
ProtClustDB	CLSN2688488.
Gene expression databases
ArrayExpress	Q9LRP1.
Genevestigator	Q9LRP1.
Family and domain databases
InterPro	IPR000727. T_SNARE_dom. [Graphical view]
PROSITE	PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPS13_ARATH

Accession

Primary (citable) accession number: Q9LRP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program