Q9LRM7 (NCED6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic
Short name=AtNCED6
EC=1.13.11.51

Gene names

Name:	NCED6
Ordered Locus Names:	At3g24220
ORF Names:	MUJ8.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy. Ref.3 Ref.5
Catalytic activity	A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal. 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al. 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subcellular location	Plastid › chloroplast stroma. Note: Partially bound to the thylakoid. Ref.4
Tissue specificity	Expressed before fertilization in male and female gametophytes, and then immediately after pollination, restricted to seed endosperm. Ref.4 Ref.5
Developmental stage	Expressed in seeds at early and mid-maturation stages. Ref.5
Induction	Low induction by drought stress. Ref.3 Ref.4
Disruption phenotype	Plants exhibit abscisic-acid-deficient phenotypes in seeds, but not in vegetative tissues. Ref.3
Sequence similarities	Belongs to the carotenoid oxygenase family.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Traceable author statement Ref.4. Source: TAIR response to red light Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay Ref.4. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay Ref.4. Source: TAIR
Molecular function	9-cis-epoxycarotenoid dioxygenase activity Inferred from direct assay Ref.3. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 577		9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic		PRO_0000285995
Sites
	Metal binding	276	1	Iron By similarity
	Metal binding	325	1	Iron By similarity
	Metal binding	390	1	Iron By similarity
	Metal binding	563	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LRM7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 515E0A1B321B4FE1	FASTA	577	63,821
	10 20 30 40 50 60 MQHSLRSDLL PTKTSPRSHL LPQPKNANIS RRILINPFKI PTLPDLTSPV PSPVKLKPTY 70 80 90 100 110 120 PNLNLLQKLA ATMLDKIESS IVIPMEQNRP LPKPTDPAVQ LSGNFAPVNE CPVQNGLEVV 130 140 150 160 170 180 GQIPSCLKGV YIRNGANPMF PPLAGHHLFD GDGMIHAVSI GFDNQVSYSC RYTKTNRLVQ 190 200 210 220 230 240 ETALGRSVFP KPIGELHGHS GLARLALFTA RAGIGLVDGT RGMGVANAGV VFFNGRLLAM 250 260 270 280 290 300 SEDDLPYQVK IDGQGDLETI GRFGFDDQID SSVIAHPKVD ATTGDLHTLS YNVLKKPHLR 310 320 330 340 350 360 YLKFNTCGKK TRDVEITLPE PTMIHDFAIT ENFVVIPDQQ MVFKLSEMIR GGSPVIYVKE 370 380 390 400 410 420 KMARFGVLSK QDLTGSDINW VDVPDCFCFH LWNAWEERTE EGDPVIVVIG SCMSPPDTIF 430 440 450 460 470 480 SESGEPTRVE LSEIRLNMRT KESNRKVIVT GVNLEAGHIN RSYVGRKSQF VYIAIADPWP 490 500 510 520 530 540 KCSGIAKVDI QNGTVSEFNY GPSRFGGEPC FVPEGEGEED KGYVMGFVRD EEKDESEFVV 550 560 570 VDATDMKQVA AVRLPERVPY GFHGTFVSEN QLKEQVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis." Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K. Plant J. 27:325-333(2001) [PubMed: 11532178] [Abstract] Cited for: FUNCTION, INDUCTION BY DROUGHT STRESS, DISRUPTION PHENOTYPE.
[4]	"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family." Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R. Plant J. 35:44-56(2003) [PubMed: 12834401] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY DROUGHT STRESS.
[5]	"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy." Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A. Plant J. 45:309-319(2006) [PubMed: 16412079] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028621 Genomic DNA. Translation: BAB01363.1. CP002686 Genomic DNA. Translation: AEE76875.1.
IPI	IPI00526624.
RefSeq	NP_189064.1. NM_113327.2.
UniGene	At.37572.
3D structure databases
ProteinModelPortal	Q9LRM7.
SMR	Q9LRM7. Positions 62-576.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LRM7.
Proteomic databases
PRIDE	Q9LRM7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G24220.1; AT3G24220.1; AT3G24220.
GeneID	822008.
GenomeReviews	Gene locus AT3G24220 in contig BA000014_GR.
KEGG	ath:AT3G24220.
NMPDR	fig|3702.1.peg.14682.
Organism-specific databases
TAIR	At3g24220.
Phylogenomic databases
eggNOG	KOG1285.
GeneTree	EPGT00050000007653.
HOGENOM	HBG559594.
InParanoid	Q9LRM7.
OMA	PKCSGIA.
PhylomeDB	Q9LRM7.
ProtClustDB	PLN02258.
Gene expression databases
Genevestigator	Q9LRM7.
Family and domain databases
InterPro	IPR004294. Carotenoid_Oase. [Graphical view]
KO	K09840.
PANTHER	PTHR10543. Carotenoid_Oase. 1 hit.
Pfam	PF03055. RPE65. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCED6_ARATH

Accession

Primary (citable) accession number: Q9LRM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program