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Q9LRM7 (NCED6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic

Short name=AtNCED6
EC=1.13.11.51
Gene names
Name:NCED6
Ordered Locus Names:At3g24220
ORF Names:MUJ8.12
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy. Ref.3 Ref.5

Catalytic activity

A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal.

9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al.

9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Subcellular location

Plastidchloroplast stroma. Note: Partially bound to the thylakoid. Ref.4

Tissue specificity

Expressed before fertilization in male and female gametophytes, and then immediately after pollination, restricted to seed endosperm. Ref.4 Ref.5

Developmental stage

Expressed in seeds at early and mid-maturation stages. Ref.5

Induction

Low induction by drought stress. Ref.3 Ref.4

Disruption phenotype

Plants exhibit abscisic-acid-deficient phenotypes in seeds, but not in vegetative tissues. Ref.3

Sequence similarities

Belongs to the carotenoid oxygenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 5779-cis-epoxycarotenoid dioxygenase NCED6, chloroplasticPRO_0000285995

Sites

Metal binding2761Iron By similarity
Metal binding3251Iron By similarity
Metal binding3901Iron By similarity
Metal binding5631Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LRM7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 515E0A1B321B4FE1

FASTA57763,821
        10         20         30         40         50         60 
MQHSLRSDLL PTKTSPRSHL LPQPKNANIS RRILINPFKI PTLPDLTSPV PSPVKLKPTY 

        70         80         90        100        110        120 
PNLNLLQKLA ATMLDKIESS IVIPMEQNRP LPKPTDPAVQ LSGNFAPVNE CPVQNGLEVV 

       130        140        150        160        170        180 
GQIPSCLKGV YIRNGANPMF PPLAGHHLFD GDGMIHAVSI GFDNQVSYSC RYTKTNRLVQ 

       190        200        210        220        230        240 
ETALGRSVFP KPIGELHGHS GLARLALFTA RAGIGLVDGT RGMGVANAGV VFFNGRLLAM 

       250        260        270        280        290        300 
SEDDLPYQVK IDGQGDLETI GRFGFDDQID SSVIAHPKVD ATTGDLHTLS YNVLKKPHLR 

       310        320        330        340        350        360 
YLKFNTCGKK TRDVEITLPE PTMIHDFAIT ENFVVIPDQQ MVFKLSEMIR GGSPVIYVKE 

       370        380        390        400        410        420 
KMARFGVLSK QDLTGSDINW VDVPDCFCFH LWNAWEERTE EGDPVIVVIG SCMSPPDTIF 

       430        440        450        460        470        480 
SESGEPTRVE LSEIRLNMRT KESNRKVIVT GVNLEAGHIN RSYVGRKSQF VYIAIADPWP 

       490        500        510        520        530        540 
KCSGIAKVDI QNGTVSEFNY GPSRFGGEPC FVPEGEGEED KGYVMGFVRD EEKDESEFVV 

       550        560        570 
VDATDMKQVA AVRLPERVPY GFHGTFVSEN QLKEQVF 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis."
Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.
Plant J. 27:325-333(2001) [PubMed: 11532178] [Abstract]
Cited for: FUNCTION, INDUCTION BY DROUGHT STRESS, DISRUPTION PHENOTYPE.
[4]"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family."
Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R.
Plant J. 35:44-56(2003) [PubMed: 12834401] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY DROUGHT STRESS.
[5]"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy."
Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A.
Plant J. 45:309-319(2006) [PubMed: 16412079] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB028621 Genomic DNA. Translation: BAB01363.1.
CP002686 Genomic DNA. Translation: AEE76875.1.
IPIIPI00526624.
RefSeqNP_189064.1. NM_113327.2.
UniGeneAt.37572.

3D structure databases

ProteinModelPortalQ9LRM7.
SMRQ9LRM7. Positions 62-576.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9LRM7.

Proteomic databases

PRIDEQ9LRM7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G24220.1; AT3G24220.1; AT3G24220.
GeneID822008.
GenomeReviewsGene locus AT3G24220 in contig BA000014_GR.
KEGGath:AT3G24220.
NMPDRfig|3702.1.peg.14682.

Organism-specific databases

TAIRAt3g24220.

Phylogenomic databases

eggNOGKOG1285.
GeneTreeEPGT00050000007653.
HOGENOMHBG559594.
InParanoidQ9LRM7.
OMAPKCSGIA.
PhylomeDBQ9LRM7.
ProtClustDBPLN02258.

Gene expression databases

GenevestigatorQ9LRM7.

Family and domain databases

InterProIPR004294. Carotenoid_Oase.
[Graphical view]
KOK09840.
PANTHERPTHR10543. Carotenoid_Oase. 1 hit.
PfamPF03055. RPE65. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCED6_ARATH
AccessionPrimary (citable) accession number: Q9LRM7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2000
Last modified: November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families