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Protein

Serine/threonine-protein phosphatase BSU1

Gene

BSU1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase that acts as a positive regulator of brassinosteroid (BR) signaling (PubMed:14977918, PubMed:21855796). Dephosphorylates BES1, a transcription factor that regulates the expression of BR-response genes, thereby playing an important role in the regulation of response to BRs (PubMed:14977918). Inactivates the negative regulator of BR signaling ASK7/BIN2 by dephosphorylation at 'Tyr-200' (PubMed:21855796).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by phosphorylation at Ser-764 by CDG1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi510Manganese 1By similarity1
Metal bindingi512Manganese 1By similarity1
Metal bindingi544Manganese 1By similarity1
Metal bindingi544Manganese 2By similarity1
Metal bindingi576Manganese 2By similarity1
Active sitei577Proton donorBy similarity1
Metal bindingi629Manganese 2By similarity1
Metal bindingi707Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: InterPro
  • positive regulation of brassinosteroid mediated signaling pathway Source: UniProtKB
  • regulation of protein localization Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase BSU1 (EC:3.1.3.16)
Alternative name(s):
Bri1 suppressor protein 1
Gene namesi
Name:BSU1
Ordered Locus Names:At1g03445
ORF Names:F21B7.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03445.
TAIRilocus:2825042. AT1G03445.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589041 – 793Serine/threonine-protein phosphatase BSU1Add BLAST793

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395Phosphoserine1 Publication1
Modified residuei444Phosphoserine1 Publication1
Modified residuei764Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated at Ser-395 and Ser-444. Phosphorylated at Ser-764 by CDG1 and CDL1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LR78.

PTM databases

iPTMnetiQ9LR78.

Expressioni

Tissue specificityi

Mainly expressed in young, elongating tissues. In young seedlings, it is expressed at the base of the hypocotyl, at the tip and most peripheral cell layers of cotyledons, and in the vascular cylinder of roots, particularly in the elongation zone and at the point of emergence of lateral roots. In mature plants, it is still present in the root vasculature, but almost completely absent in fully expanded stems and leaves. In flowers, it is mainly expressed in sepal veins, anther filaments, and in the style, suggesting that BSU1 is expressed in actively growing regions and apparently enriched in vascular tissues.1 Publication

Gene expression databases

ExpressionAtlasiQ9LR78. baseline and differential.
GenevisibleiQ9LR78. AT.

Interactioni

Subunit structurei

Interacts with CDG1, CDL1 and ASK7/BIN2.1 Publication

Protein-protein interaction databases

BioGridi24043. 4 interactors.
STRINGi3702.AT1G03445.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LR78.
SMRiQ9LR78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 109Kelch 1Add BLAST57
Repeati110 – 160Kelch 2Add BLAST51
Repeati214 – 262Kelch 3Add BLAST49
Repeati264 – 314Kelch 4Add BLAST51
Repeati329 – 388Kelch 5Add BLAST60

Sequence similaritiesi

Belongs to the PPP phosphatase family. BSU subfamily.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
KOG0379. Eukaryota.
ENOG410Z3NZ. LUCA.
HOGENOMiHOG000246464.
InParanoidiQ9LR78.
KOiK14501.
OMAiHECVIDG.
OrthoDBiEOG09361248.
PhylomeDBiQ9LR78.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.130.10.80. 1 hit.
3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR015916. Gal_Oxidase_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR012391. Ser/Thr_prot_Pase_BSU1.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PIRSFiPIRSF036363. PPP_BSU1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LR78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPDQSYQYP SPSYESIQTF YDTDEDWPGP RCGHTLTAVF VNNSHQLILF
60 70 80 90 100
GGSTTAVANH NSSLPEISLD GVTNSVHSFD VLTRKWTRLN PIGDVPSPRA
110 120 130 140 150
CHAAALYGTL ILIQGGIGPS GPSDGDVYML DMTNNKWIKF LVGGETPSPR
160 170 180 190 200
YGHVMDIAAQ RWLVIFSGNN GNEILDDTWA LDTRGPFSWD RLNPSGNQPS
210 220 230 240 250
GRMYASGSSR EDGIFLLCGG IDHSGVTLGD TYGLKMDSDN VWTPVPAVAP
260 270 280 290 300
SPRYQHTAVF GGSKLHVIGG ILNRARLIDG EAVVAVLDTE TGEWVDTNQP
310 320 330 340 350
ETSASGANRQ NQYQLMRRCH HAAASFGSHL YVHGGIREDV LLDDLLVAET
360 370 380 390 400
SQSSSPEPEE DNPDNYMLLD DYLMDEPKPL SSEPEASSFI MRSTSEIAMD
410 420 430 440 450
RLAEAHNLPT IENAFYDSAI EGYVPLQHGA ETVGNRGGLV RTASLDQSTQ
460 470 480 490 500
DLHKKVISTL LRPKTWTPPA NRDFFLSYLE VKHLCDEVEK IFMNEPTLLQ
510 520 530 540 550
LKVPIKVFGD IHGQYGDLMR LFHEYGHPSV EGDITHIDYL FLGDYVDRGQ
560 570 580 590 600
HSLEIIMLLF ALKIEYPKNI HLIRGNHESL AMNRIYGFLT ECEERMGESY
610 620 630 640 650
GFEAWLKINQ VFDYLPLAAL LEKKVLCVHG GIGRAVTIEE IENIERPAFP
660 670 680 690 700
DTGSMVLKDI LWSDPTMNDT VLGIVDNARG EGVVSFGPDI VKAFLERNGL
710 720 730 740 750
EMILRAHECV IDGFERFADG RLITVFSATN YCGTAQNAGA ILVIGRDMVI
760 770 780 790
YPKLIHPHPP PISSSEEDYT DKAWMQELNI EMPPTPARGE SSE
Length:793
Mass (Da):87,789
Last modified:March 15, 2004 - v2
Checksum:i07A083BD780EAA79
GO

Sequence cautioni

The sequence AAF86539 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY372269 mRNA. Translation: AAR19789.1.
AC002560 Genomic DNA. Translation: AAF86539.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27572.1.
RefSeqiNP_171844.6. NM_100227.6.
UniGeneiAt.49845.

Genome annotation databases

EnsemblPlantsiAT1G03445.1; AT1G03445.1; AT1G03445.
GeneIDi838804.
GrameneiAT1G03445.1; AT1G03445.1; AT1G03445.
KEGGiath:AT1G03445.

Similar proteinsi

Entry informationi

Entry nameiBSU1_ARATH
AccessioniPrimary (citable) accession number: Q9LR78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: August 30, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families