Reviewed, UniProtKB/Swiss-Prot Q9LR78 (BSU1_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase BSU1
    EC=3.1.3.16
Alternative name(s):
    Bri1 suppressor protein 1

Gene names

Name:	BSU1
Ordered Locus Names:	At1g03445, At1g03450
ORF Names:	F21B7.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphatase that acts as a positive regulator of brassinolide signaling. Dephosphorylates BES1, a transcription factor that regulates the expression of brassinolide-response genes, thereby playing an important role in the regulation of response to brassinosteroids. Ref.1
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate. Ref.1
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subcellular location	Nucleus Ref.1.
Tissue specificity	Mainly expressed in young, elongating tissues. In young seedlings, it is expressed at the base of the hypocotyl, at the tip and most peripheral cell layers of cotyledons, and in the vascular cylinder of roots, particularly in the elongation zone and at the point of emergence of lateral roots. In mature plants, it is still present in the root vasculature, but almost completely absent in fully expanded stems and leaves. In flowers, it is mainly expressed in sepal veins, anther filaments, and in the style, suggesting that BSU1 is expressed in actively growing regions and apparently enriched in vascular tissues. Ref.1
Sequence similarities	Belongs to the PPP phosphatase family. BSU subfamily. Contains 5 Kelch repeats.
Sequence caution	The sequence AAF86539.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Kelch repeat Repeat
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	brassinosteroid mediated signaling pathway Ref.1 Inferred from genetic interaction. Source: TAIR regulation of protein localization Inferred from direct assay. Source: TAIR
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: TAIR protein serine/threonine phosphatase activity Ref.1 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 793	793	Serine/threonine-protein phosphatase BSU1		PRO_0000058904
Regions
	Repeat	53 – 109	57	Kelch 1
	Repeat	110 – 160	51	Kelch 2
	Repeat	214 – 262	49	Kelch 3
	Repeat	264 – 314	51	Kelch 4
	Repeat	329 – 388	60	Kelch 5
Sites
	Active site	577	1	Proton donor By similarity
	Metal binding	510	1	Iron By similarity
	Metal binding	512	1	Iron By similarity
	Metal binding	544	1	Iron By similarity
	Metal binding	544	1	Manganese By similarity
	Metal binding	576	1	Manganese By similarity
	Metal binding	629	1	Manganese By similarity
	Metal binding	707	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LR78-1 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 07A083BD780EAA79	FASTA	793	87,789
	10 20 30 40 50 60 MAPDQSYQYP SPSYESIQTF YDTDEDWPGP RCGHTLTAVF VNNSHQLILF GGSTTAVANH 70 80 90 100 110 120 NSSLPEISLD GVTNSVHSFD VLTRKWTRLN PIGDVPSPRA CHAAALYGTL ILIQGGIGPS 130 140 150 160 170 180 GPSDGDVYML DMTNNKWIKF LVGGETPSPR YGHVMDIAAQ RWLVIFSGNN GNEILDDTWA 190 200 210 220 230 240 LDTRGPFSWD RLNPSGNQPS GRMYASGSSR EDGIFLLCGG IDHSGVTLGD TYGLKMDSDN 250 260 270 280 290 300 VWTPVPAVAP SPRYQHTAVF GGSKLHVIGG ILNRARLIDG EAVVAVLDTE TGEWVDTNQP 310 320 330 340 350 360 ETSASGANRQ NQYQLMRRCH HAAASFGSHL YVHGGIREDV LLDDLLVAET SQSSSPEPEE 370 380 390 400 410 420 DNPDNYMLLD DYLMDEPKPL SSEPEASSFI MRSTSEIAMD RLAEAHNLPT IENAFYDSAI 430 440 450 460 470 480 EGYVPLQHGA ETVGNRGGLV RTASLDQSTQ DLHKKVISTL LRPKTWTPPA NRDFFLSYLE 490 500 510 520 530 540 VKHLCDEVEK IFMNEPTLLQ LKVPIKVFGD IHGQYGDLMR LFHEYGHPSV EGDITHIDYL 550 560 570 580 590 600 FLGDYVDRGQ HSLEIIMLLF ALKIEYPKNI HLIRGNHESL AMNRIYGFLT ECEERMGESY 610 620 630 640 650 660 GFEAWLKINQ VFDYLPLAAL LEKKVLCVHG GIGRAVTIEE IENIERPAFP DTGSMVLKDI 670 680 690 700 710 720 LWSDPTMNDT VLGIVDNARG EGVVSFGPDI VKAFLERNGL EMILRAHECV IDGFERFADG 730 740 750 760 770 780 RLITVFSATN YCGTAQNAGA ILVIGRDMVI YPKLIHPHPP PISSSEEDYT DKAWMQELNI 790 EMPPTPARGE SSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis." Mora-Garcia S., Vert G., Yin Y., Cano-Delgado A., Cheong H., Chory J. Genes Dev. 18:448-460(2004) [PubMed: 14977918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed: 17368080] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY372269 mRNA. Translation: AAR19789.1. AC002560 Genomic DNA. Translation: AAF86539.1. Sequence problems.
IPI	IPI00517992.
RefSeq	NP_171844.6.
UniGene	At.49845
3D structure databases
SMR	Q9LR78. Positions 455-758.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LR78.
Genome annotation databases
GeneID	838804.
GenomeReviews	Gene locus AT1G03445 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g03445.
Phylogenomic databases
eggNOG	KOG0379.
HOGENOM	HBG719569.
InParanoid	Q9LR78.
PhylomeDB	Q9LR78.
Enzyme and pathway databases
BRENDA	3.1.3.16. 302.
Gene expression databases
ArrayExpress	Q9LR78.
Genevestigator	Q9LR78.
GermOnline	AT1G03445. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011043. Gal_Oxase/kelch_b-propeller. IPR015915. Kelch-typ_b-propeller. IPR004843. M-pesterase. IPR006186. Ser/Thr-sp_prot-phosphatase. IPR012391. Ser/Thr_prot_Pase_BSU1. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 2 hits.
PANTHER	PTHR11668. T_phtase_apaH. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF036363. PPP_BSU1. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BSU1_ARATH

Accession

Primary (citable) accession number: Q9LR78

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 15, 2004
Last modified:	February 9, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)