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Reviewed, UniProtKB/Swiss-Prot Q9LR75 (HEM6_ARATH)

Last modified February 9, 2010. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Coproporphyrinogen-III oxidase, chloroplastic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3
Alternative name(s):
    Protein LESION INITIATION 2
Gene names
Name: CPX
Synonyms: CPO, HEMF, LIN2
Ordered Locus Names: At1g03475
ORF Names: F21B7.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity.

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast Probable.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast Potential
Chain49 – 386338Coproporphyrinogen-III oxidase, chloroplastic
PRO_0000006031

Regions

Region125 – 13410Important for dimerization By similarity
Region190 – 1923Substrate binding By similarity
Region326 – 36136Important for dimerization By similarity
Region344 – 3496Substrate binding By similarity

Sites

Active site1881Proton donor By similarity
Binding site1741Substrate By similarity
Site2611Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LR75-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B82BD6787643D0B8

FASTA38643,796
        10         20         30         40         50         60 
MASHSSTLLS SPTFAPFSSH RLHYSPNPST LRFSRPIRNK PNLALRCSVS IEKEVPETER 

        70         80         90        100        110        120 
PFTFLRDSDD VTPSSSSSSV RARFETMIRA AQDSVCDAIE AIEGGPKFKE DVWSRPGGGG 

       130        140        150        160        170        180 
GISRVLQDGN VFEKAGVNVS VVYGVMPPEA YRAAKGSASD QKPGPVPFFA AGVSSVLHPK 

       190        200        210        220        230        240 
NPFAPTLHFN YRYFETDAPK DVPGAPRQWW FGGGTDFTPA YIFEEDVKHF HSIQKQACDK 

       250        260        270        280        290        300 
FDPSFYPRFK KWCDDYFYIK HRDERRGLGG IFFDDLNDYD QEMLLSFATE CANSVVPAYI 

       310        320        330        340        350        360 
PIVEKRKDME FTEQHKAWQQ LRRGRYVEFN LVYDRGTTFG LKTGGRIESI LVSLPLSARW 

       370        380 
EYDHKPEEGT EEWKLLDACI NPKEWI 

« Hide

References

« Hide 'large scale' references
[1]"A deficiency of coproporphyrinogen III oxidase causes lesion formation in Arabidopsis."
Ishikawa A., Okamoto H., Iwasaki Y., Asahi T.
Plant J. 27:89-99(2001) [PubMed: 11489187] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation of genes and subcellular localization of tetrapyrrole biosynthetic enzymes from Arabidopsis."
Tan F.C.
Thesis (2001), University of Cambridge, United Kingdom
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
[3]"Molecular characterisation of coproporphyrinogen oxidase from Glycine max and Arabidopsis thaliana."
Santana M.A., Tan F.C., Smith A.G.
Plant Physiol. Biochem. 40:289-298(2002)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
[4]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-386.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB044394 mRNA. Translation: BAB61876.1.
AJ420796 mRNA. Translation: CAD12661.1.
AC002560 Genomic DNA. Translation: AAF86536.1.
AF375424 mRNA. Translation: AAK53008.1. Different initiation.
AY113166 mRNA. Translation: AAM47469.1. Different initiation.
IPIIPI00519439.
PIRC86166.
RefSeqNP_171847.4.
UniGeneAt.19140
Rra.9658

3D structure databases

SMRQ9LR75. Positions 52-386.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9LR75.

Proteomic databases

PRIDEQ9LR75.
ProMEXQ9LR75.

Genome annotation databases

GeneID839489.
GenomeReviewsGene locus AT1G03475 in contig CT485782_GR.

Organism-specific databases

TAIRAt1g03475.

Phylogenomic databases

eggNOGKOG1518.
HOGENOMHBG631180.
InParanoidQ9LR75.
PhylomeDBQ9LR75.

Enzyme and pathway databases

BioCycMetaCyc:AT1G03475-MONOMER.
BRENDA1.3.3.3. 302.

Gene expression databases

GenevestigatorQ9LR75.
GermOnlineAT1G03475. Arabidopsis thaliana.

Family and domain databases

InterProIPR001260. Coprogen_oxidas.
IPR018375. Coproporphyrinogen-3_ox_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_ARATH
AccessionPrimary (citable) accession number: Q9LR75
Secondary accession number(s): Q546I5, Q94JR5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents