Reviewed, UniProtKB/Swiss-Prot Q9LR75 (HEM6_ARATH)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, chloroplastic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3
Alternative name(s):
    Protein LESION INITIATION 2

Gene names

Name:	CPX
Synonyms:	CPO, HEMF, LIN2
Ordered Locus Names:	At1g03475
ORF Names:	F21B7.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.
Subcellular location	Plastid › chloroplast Probable.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 386	338	Coproporphyrinogen-III oxidase, chloroplastic		PRO_0000006031

Sequences

	Sequence		Length	Mass (Da)
	Q9LR75-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B82BD6787643D0B8	FASTA	386	43,796
	10 20 30 40 50 60 MASHSSTLLS SPTFAPFSSH RLHYSPNPST LRFSRPIRNK PNLALRCSVS IEKEVPETER 70 80 90 100 110 120 PFTFLRDSDD VTPSSSSSSV RARFETMIRA AQDSVCDAIE AIEGGPKFKE DVWSRPGGGG 130 140 150 160 170 180 GISRVLQDGN VFEKAGVNVS VVYGVMPPEA YRAAKGSASD QKPGPVPFFA AGVSSVLHPK 190 200 210 220 230 240 NPFAPTLHFN YRYFETDAPK DVPGAPRQWW FGGGTDFTPA YIFEEDVKHF HSIQKQACDK 250 260 270 280 290 300 FDPSFYPRFK KWCDDYFYIK HRDERRGLGG IFFDDLNDYD QEMLLSFATE CANSVVPAYI 310 320 330 340 350 360 PIVEKRKDME FTEQHKAWQQ LRRGRYVEFN LVYDRGTTFG LKTGGRIESI LVSLPLSARW 370 380 EYDHKPEEGT EEWKLLDACI NPKEWI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A deficiency of coproporphyrinogen III oxidase causes lesion formation in Arabidopsis." Ishikawa A., Okamoto H., Iwasaki Y., Asahi T. Plant J. 27:89-99(2001) [PubMed: 11489187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of genes and subcellular localization of tetrapyrrole biosynthetic enzymes from Arabidopsis." Tan F.C. Thesis (2001), University of Cambridge, United Kingdom Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[3]	"Molecular characterisation of coproporphyrinogen oxidase from Glycine max and Arabidopsis thaliana." Santana M.A., Tan F.C., Smith A.G. Plant Physiol. Biochem. 40:289-298(2002) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-386. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB044394 mRNA. Translation: BAB61876.1. AJ420796 mRNA. Translation: CAD12661.1. AC002560 Genomic DNA. Translation: AAF86536.1. AF375424 mRNA. Translation: AAK53008.1. Different initiation. AY113166 mRNA. Translation: AAM47469.1. Different initiation.
IPI	IPI00519439.
PIR	C86166.
RefSeq	NP_171847.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LR75.
Proteomic databases
PRIDE	Q9LR75.
ProMEX	Q9LR75.
Genome annotation databases
GeneID	839489.
GenomeReviews	Gene locus AT1G03475 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g03475.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G03475-MON.
BRENDA	1.3.3.3. 302.
Gene expression databases
Genevestigator	Q9LR75.
GermOnline	AT1G03475. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_ARATH

Accession

Primary (citable) accession number: Q9LR75
Secondary accession number(s): Q546I5, Q94JR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	October 13, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)