Q9LR65 (P2C01_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 1
Short name=AtPP2C01
EC=3.1.3.16

Alternative name(s):
AtPPC6;6

Gene names

Name:	PPC6-6
Ordered Locus Names:	At1g03590
ORF Names:	F21B7.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as negative regulator of GCN5. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Interacts with GCN5. Ref.4
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Probable protein phosphatase 2C 1		PRO_0000367935
Regions
	Domain	90 – 341	252	PP2C-like
Sites
	Metal binding	95	1	Manganese 1 By similarity
	Metal binding	95	1	Manganese 2 By similarity
	Metal binding	96	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	307	1	Manganese 2 By similarity
	Metal binding	353	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LR65 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F264E7DA32C7E472	FASTA	462	50,975
	10 20 30 40 50 60 MGGCISKTSW SNEEPMHRPC LGMGCCGSKM GKRGFSDRMV SLHNLVSIPN RIIGNGKSRS 70 80 90 100 110 120 SCIFTQQGRK GINQDAMIVW EDFMSKDVTF CGVFDGHGPH GHLVARKVRD SLPVKLLSLL 130 140 150 160 170 180 NSIKSKQNGP IGTRASKSDS LEAEKEESTE EDKLNFLWEE AFLKSFNAMD KELRSHPNLE 190 200 210 220 230 240 CFCSGCTAVT IIKQGSNLYM GNIGDSRAIL GSKDSNDSMI AVQLTVDLKP DLPREAERIK 250 260 270 280 290 300 QCKGRVFALQ DEPEVSRVWL PFDNAPGLAM ARAFGDFCLK DYGVISIPEF SHRVLTDRDQ 310 320 330 340 350 360 FIVLASDGVW DVLSNEEVVE VVASATSRAS AARLVVDSAV REWKLKYPTS KMDDCAVVCL 370 380 390 400 410 420 FLDGRMDSET SDNEEQCFSS ATNAVESDES QGAEPCLQRN VTVRSLSTDQ ENNSYGKVIA 430 440 450 460 EADNAEKEKT REGEQNWSGL EGVTRVNSLV QLPRFPGEEP KT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana." Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-462.
[4]	"Characterization of a phosphatase 2C protein as an interacting partner of the histone acetyltransferase GCN5 in Arabidopsis." Servet C., Benhamed M., Latrasse D., Kim W., Delarue M., Zhou D.-X. Biochim. Biophys. Acta 1779:376-382(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GCN5.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002560 Genomic DNA. Translation: AAF86530.1. CP002684 Genomic DNA. Translation: AEE27587.1. AB079670 mRNA. Translation: BAB84699.1.
IPI	IPI00518029.
PIR	T00901.
RefSeq	NP_171856.4. NM_100239.7.
UniGene	At.42536. At.44796.
3D structure databases
ProteinModelPortal	Q9LR65.
SMR	Q9LR65. Positions 67-358.
ModBase	Search...
Proteomic databases
PaxDb	Q9LR65.
PRIDE	Q9LR65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G03590.1; AT1G03590.1; AT1G03590.
GeneID	839447.
KEGG	ath:AT1G03590.
Organism-specific databases
TAIR	At1g03590.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233897.
InParanoid	Q9LR65.
OMA	DYGVISI.
PhylomeDB	Q9LR65.
ProtClustDB	CLSN2719829.
Gene expression databases
Genevestigator	Q9LR65.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C01_ARATH

Accession

Primary (citable) accession number: Q9LR65
Secondary accession number(s): Q8SBC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program