Reviewed, UniProtKB/Swiss-Prot Q9LR47 (IP5P3_ARATH)

Names and origin

Protein names

Recommended name:
    Type I inositol-1,4,5-trisphosphate 5-phosphatase CVP2
    EC=3.1.3.56
Alternative name(s):
    Protein COTYLEDON VASCULAR PATTERN 2

Gene names

Name:	CVP2
Ordered Locus Names:	At1g05470
ORF Names:	T25N20.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the patterning of procambium and during the differentiation of vascular tissues. Acts before the acquisition of preprocambial identity. Seems to be also involved in the abscisic acid (ABA) signaling pathway. Ref.2 Ref.3
Catalytic activity	D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate. 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate.
Tissue specificity	Broadly expressed in emerging organs. Mostly localized in procambium of growing organs. Restricted to vascular differentiating cells of young organs. Ref.3
Developmental stage	During early stages of embryogenesis, broadly expressed throughout globular and torpedo stages. At late torpedo stage, strongly expressed in developing vascular cells and weakly expressed in surrounding cells. By the walking-stick stage, restricted to incipient vascular cells of the apical loop, the midvein of the cotyledon and the root vasculature. In emerging leaves, strongly expressed in procambium and weakly expressed in areole cells. Limited to developing vascular cells in later stage of leaf development. In roots, expressed in procambium of the elongating zone and in immature vascular cells of the root differentiation zone. During inflorescence development, broadly expressed in young floral buds, and gradually restricted to procambium of cauline leaves, sepals, petals, gynoecia and anthers. Ref.3
Sequence similarities	Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type I family.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway Differentiation
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid mediated signaling Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cotyledon vascular tissue pattern formation Ref.3 Inferred from mutant phenotype. Source: TAIR inositol phosphate dephosphorylation Ref.3 Inferred from mutant phenotype. Source: TAIR inositol phosphate-mediated signaling Ref.3 Inferred by curator. Source: TAIR inositol trisphosphate metabolic process Ref.3 Inferred from mutant phenotype. Source: TAIR leaf vascular tissue pattern formation Ref.3 Inferred from mutant phenotype. Source: TAIR procambium histogenesis Ref.3 Inferred from mutant phenotype. Source: TAIR
Molecular function	inositol-polyphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Type I inositol-1,4,5-trisphosphate 5-phosphatase CVP2		PRO_0000209724
Regions
	Region	450 – 465	16	Catalytic 1 Potential
	Region	530 – 545	16	Catalytic 2 Potential
	Compositional bias	228 – 325	98	Ser-rich
Experimental info
	Mutagenesis	535	1	A → V in cvp2-2; induces an open vein network. Ref.3
	Mutagenesis	567	1	G → D in cvp2-1; induces an open vein network. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LR47-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 042A743C7FB00805	FASTA	609	69,880
	10 20 30 40 50 60 MKLAWSKKMV RKWFNIKSKT EEFQADDPSS AGIEVEHRSS FSAEKAPSTI KNTKTEKLSK 70 80 90 100 110 120 NWEQQARQRR MNYENPRIID VQNYSIFVAT WNVAGRSPPS DLNLDEWLHS SAPADIYVLG 130 140 150 160 170 180 FQEIVPLNAG NVLGAEDNGP AQKWLSLIRK TLNNRPGTSG TSGYHTPSPI PVPMAELDAD 190 200 210 220 230 240 FSGSTRQKNS TFFHRRSFQT PSSTWNDPSI PQPGLDRRFS VCDRVFFSHR PSDFDPSFRG 250 260 270 280 290 300 SSSSHRPSDY SRRPSDYSRR PSDYSRRPSD YSRRPSDSRP SDYSRPSDYY SRPSDYSRPS 310 320 330 340 350 360 DFSRSSDDDN GLGDSPSTVL YSPGSAANEN GYRIPWNSSQ YCLVASKQMV GVFLTIWVKS 370 380 390 400 410 420 ELREHVKNMK VSCVGRGLMG YLGNKGSISI SMLLHQTSFC FVCTHLTSGQ KEGDELKRNS 430 440 450 460 470 480 DVMEILKKTR FPRVKSSEEE KSPENILQHD RVIWLGDLNY RIALSYRSAK ALVEMQNWRA 490 500 510 520 530 540 LLENDQLRIE QKRGHVFKGW NEGKIYFPPT YKYSRNSDRY SGDDLHPKEK RRTPAWCDRI 550 560 570 580 590 600 LWFGEGLHQL SYVRGESRFS DHRPVYGIFC AEVESAHNRI KRTTSYSASR VQAEELLPYS RGYTELSFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Genetic regulation of vascular tissue patterning in Arabidopsis." Carland F.M., Berg B.L., FitzGerald J.N., Jinamornphongs S., Nelson T., Keith B. Plant Cell 11:2123-2137(1999) [PubMed: 10559439] [Abstract] Cited for: FUNCTION, MUTANT CVP2-1.
[3]	"COTYLEDON VASCULAR PATTERN2-mediated inositol (1,4,5) triphosphate signal transduction is essential for closed venation patterns of Arabidopsis foliar organs." Carland F.M., Nelson T. Plant Cell 16:1263-1275(2004) [PubMed: 15100402] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF ALA-535 AND GLY-567.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC005106 Genomic DNA. Translation: AAF79735.1.
IPI	IPI00517491.
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
ModBase	Search...
Proteomic databases
PRIDE	Q9LR47.
Genome annotation databases
GenomeReviews	Gene locus AT1G05470 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g05470.
Enzyme and pathway databases
BRENDA	3.1.3.56. 302.
Gene expression databases
GermOnline	AT1G05470. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IP5P3_ARATH

Accession

Primary (citable) accession number: Q9LR47

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 1, 2000
Last modified:	February 10, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)