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Protein

Tryptophan aminotransferase-related protein 1

Gene

TAR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably involved in auxin production. TAA1, TAR1 and TAR2 are required for proper embryo patterning.1 Publication

Catalytic activityi

L-tryptophan + 2-oxoglutarate = (indol-3-yl)pyruvate + L-glutamate.
L-tryptophan + pyruvate = indole-3-pyruvate + L-alanine.

Cofactori

Enzyme regulationi

Inhibited by L-kynurenine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591Pyridoxal phosphateBy similarity
Binding sitei169 – 1691Pyridoxal phosphateBy similarity
Binding sitei190 – 1901Pyridoxal phosphateBy similarity
Binding sitei224 – 2241Pyridoxal phosphateBy similarity

GO - Molecular functioni

  • carbon-sulfur lyase activity Source: InterPro
  • L-tryptophan:2-oxoglutarate aminotransferase activity Source: TAIR
  • L-tryptophan:pyruvate aminotransferase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Auxin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.27. 399.
UniPathwayiUPA00151.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan aminotransferase-related protein 1 (EC:2.6.1.27, EC:2.6.1.99)
Gene namesi
Name:TAR1
Ordered Locus Names:At1g23320
ORF Names:F26F24.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G23320.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Tryptophan aminotransferase-related protein 1PRO_0000411674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161N6-(pyridoxal phosphate)lysineSequence Analysis

Expressioni

Tissue specificityi

Very low expression in seedlings.1 Publication

Inductioni

Slightly up-regulated by ethylene.1 Publication

Gene expression databases

GenevestigatoriQ9LR29.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G23320.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LR29.
SMRiQ9LR29. Positions 21-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 1022Pyridoxal phosphate bindingBy similarity
Regioni190 – 1934Pyridoxal phosphate bindingBy similarity
Regioni213 – 2164Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the alliinase family.Curated

Phylogenomic databases

eggNOGiNOG284125.
HOGENOMiHOG000237549.
InParanoidiQ9LR29.
KOiK16903.
OMAiCENSKKS.

Family and domain databases

Gene3Di2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF04864. Alliinase_C. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LR29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVGCENSKK SDSGSNEDKS LSDDIINLDQ GDPTAFQEYW MKKKDRCTVV
60 70 80 90 100
IPAWDLMSYF SDTKNVCWFL EPELEKAIKA LHGAIGNAAT EERYIVVGTG
110 120 130 140 150
SSQLCQAALF ALSSLSEVKP VSIVAAVPYY STYVEEASYL QSTLYKWEGD
160 170 180 190 200
ARTFDKKGPY IELVTSPNNP DGIMREPVVN RREGGKVIHD LAYYWPHYTP
210 220 230 240 250
ITRRQDHDLM LFTFSKITGH AGSRIGWALV KDIEVAKKMV HYLTINSIGV
260 270 280 290 300
SKESQTRATT ILNELTKTCR TQSESFFEYG YEKMKSRWER LREVVESGDA
310 320 330 340 350
FTLPNYPQDF CNFFGKTLST SPAFAWLGYK EERDLGSLLK EKKVLTRGGD
360 370 380
RCGCNKRYVR VSMLSRDDDF DVSLQRLATI KDLKCVEP
Length:388
Mass (Da):44,064
Last modified:July 27, 2011 - v2
Checksum:i4B5FEA35566BBD12
GO

Sequence cautioni

The sequence AAF87014.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005292 Genomic DNA. Translation: AAF87014.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE30372.1.
RefSeqiNP_173746.1. NM_102181.1.
UniGeneiAt.51734.

Genome annotation databases

EnsemblPlantsiAT1G23320.1; AT1G23320.1; AT1G23320.
GeneIDi838941.
KEGGiath:AT1G23320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005292 Genomic DNA. Translation: AAF87014.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE30372.1.
RefSeqiNP_173746.1. NM_102181.1.
UniGeneiAt.51734.

3D structure databases

ProteinModelPortaliQ9LR29.
SMRiQ9LR29. Positions 21-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G23320.1-P.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G23320.1; AT1G23320.1; AT1G23320.
GeneIDi838941.
KEGGiath:AT1G23320.

Organism-specific databases

TAIRiAT1G23320.

Phylogenomic databases

eggNOGiNOG284125.
HOGENOMiHOG000237549.
InParanoidiQ9LR29.
KOiK16903.
OMAiCENSKKS.

Enzyme and pathway databases

UniPathwayiUPA00151.
BRENDAi2.6.1.27. 399.

Miscellaneous databases

PROiQ9LR29.

Gene expression databases

GenevestigatoriQ9LR29.

Family and domain databases

Gene3Di2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF04864. Alliinase_C. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development."
    Stepanova A.N., Robertson-Hoyt J., Yun J., Benavente L.M., Xie D.Y., Dolezal K., Schlereth A., Juergens G., Alonso J.M.
    Cell 133:177-191(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION BY ETHYLENE, GENE FAMILY, NOMENCLATURE.
  4. "Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants."
    Tao Y., Ferrer J.L., Ljung K., Pojer F., Hong F., Long J.A., Li L., Moreno J.E., Bowman M.E., Ivans L.J., Cheng Y., Lim J., Zhao Y., Ballare C.L., Sandberg G., Noel J.P., Chory J.
    Cell 133:164-176(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis."
    He W., Brumos J., Li H., Ji Y., Ke M., Gong X., Zeng Q., Li W., Zhang X., An F., Wen X., Li P., Chu J., Sun X., Yan C., Yan N., Xie D.Y., Raikhel N.
    , Yang Z., Stepanova A.N., Alonso J.M., Guo H.
    Plant Cell 23:3944-3960(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiTAR1_ARATH
AccessioniPrimary (citable) accession number: Q9LR29
Secondary accession number(s): F4I655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.