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Protein

DELLA protein GAI

Gene

GAI

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression. In contrast to RGA, it is less sensitive to GA. Its activity is probably regulated by other phytohormones such as auxin and ethylene.8 Publications

GO - Molecular functioni

GO - Biological processi

  • gibberellic acid mediated signaling pathway Source: TAIR
  • meiotic cytokinesis Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of developmental vegetative growth Source: CAFA
  • negative regulation of gene expression Source: CAFA
  • negative regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • negative regulation of leaf development Source: CAFA
  • negative regulation of trichome patterning Source: CAFA
  • phloem transport Source: TAIR
  • positive regulation of gene expression Source: CAFA
  • regulation of nitrogen utilization Source: TAIR
  • response to far red light Source: TAIR
  • response to gibberellin Source: CAFA
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processGibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein GAI
Alternative name(s):
GRAS family protein 3
Short name:
AtGRAS-3
Gibberellic acid-insensitive mutant protein
Restoration of growth on ammonia protein 2
Gene namesi
Name:GAI
Synonyms:RGA2
Ordered Locus Names:At1g14920
ORF Names:F10B6.34
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G14920.
TAIRilocus:2006747. AT1G14920.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28 – 44Missing in gai; causes a dwarf phenotype. 1 PublicationAdd BLAST17
Mutagenesisi135 – 138Missing : Does not affect nuclear localization. 1 Publication4
Mutagenesisi219 – 223Missing : Does not affect nuclear localization. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322351 – 533DELLA protein GAIAdd BLAST533

Post-translational modificationi

Phosphorylated.By similarity
Gibberellin (GA) induces dephosphorylation of GAI by TOPP4 and subsequent degradation by the proteasomal pathway.1 Publication
May be ubiquitinated, as suggested by its interaction with GID2. Ubiquitination is however unsure since in contrast to other DELLA proteins, it is not ubiquitinated and degraded upon GA application. Nevertheless, ubiquitination may be triggered by other processes.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9LQT8.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in rosette leaves, roots, stems and inflorescences of greenhouse grown.3 Publications

Gene expression databases

GenevisibleiQ9LQT8. AT.

Interactioni

Subunit structurei

Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1B (via N-terminus). Interacts with the BOI proteins BOI, BRG1, BRG2, BRG3 and NUP58 (PubMed:15155881, PubMed:15161962, PubMed:15173565, PubMed:16709201, PubMed:19037309, PubMed:23482857, PubMed:23840761). Interacts with TOPP4 (PubMed:25010794).8 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi23297. 102 interactors.
DIPiDIP-32985N.
IntActiQ9LQT8. 37 interactors.
STRINGi3702.AT1G14920.1.

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 32Combined sources4
Turni33 – 35Combined sources3
Helixi40 – 42Combined sources3
Helixi43 – 57Combined sources15
Helixi69 – 72Combined sources4
Helixi80 – 89Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZSHX-ray1.80B11-113[»]
2ZSIX-ray1.80B11-113[»]
DisProtiDP00724.
ProteinModelPortaliQ9LQT8.
SMRiQ9LQT8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LQT8.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi28 – 32DELLA motif5
Motifi50 – 54LEXLE motif5
Motifi73 – 77VHYNP motif5
Motifi271 – 275VHIID5
Motifi371 – 375LXXLL motif5

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH. Eukaryota.
ENOG410YBFY. LUCA.
HOGENOMiHOG000238577.
InParanoidiQ9LQT8.
KOiK14494.
OMAiRVEESDG.
OrthoDBiEOG0936075R.
PhylomeDBiQ9LQT8.

Family and domain databases

InterProiView protein in InterPro
IPR030006. TF_DELLA.
IPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
PANTHERiPTHR31636:SF47. PTHR31636:SF47. 1 hit.
PfamiView protein in Pfam
PF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
PROSITEiView protein in PROSITE
PS50985. GRAS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LQT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRDHHHHHH QDKKTMMMNE EDDGNGMDEL LAVLGYKVRS SEMADVAQKL
60 70 80 90 100
EQLEVMMSNV QEDDLSQLAT ETVHYNPAEL YTWLDSMLTD LNPPSSNAEY
110 120 130 140 150
DLKAIPGDAI LNQFAIDSAS SSNQGGGGDT YTTNKRLKCS NGVVETTTAT
160 170 180 190 200
AESTRHVVLV DSQENGVRLV HALLACAEAV QKENLTVAEA LVKQIGFLAV
210 220 230 240 250
SQIGAMRKVA TYFAEALARR IYRLSPSQSP IDHSLSDTLQ MHFYETCPYL
260 270 280 290 300
KFAHFTANQA ILEAFQGKKR VHVIDFSMSQ GLQWPALMQA LALRPGGPPV
310 320 330 340 350
FRLTGIGPPA PDNFDYLHEV GCKLAHLAEA IHVEFEYRGF VANTLADLDA
360 370 380 390 400
SMLELRPSEI ESVAVNSVFE LHKLLGRPGA IDKVLGVVNQ IKPEIFTVVE
410 420 430 440 450
QESNHNSPIF LDRFTESLHY YSTLFDSLEG VPSGQDKVMS EVYLGKQICN
460 470 480 490 500
VVACDGPDRV ERHETLSQWR NRFGSAGFAA AHIGSNAFKQ ASMLLALFNG
510 520 530
GEGYRVEESD GCLMLGWHTR PLIATSAWKL STN
Length:533
Mass (Da):58,927
Last modified:October 1, 2000 - v1
Checksum:i6464B8C129D79528
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10Missing in CAA72178 (PubMed:9237632).Curated1
Sequence conflicti10Missing in CAA75492 (PubMed:9389651).Curated1
Sequence conflicti208K → Q in CAA72178 (PubMed:9237632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11337 mRNA. Translation: CAA72178.1.
Y15193 Genomic DNA. Translation: CAA75492.1.
AC006917 Genomic DNA. Translation: AAF79228.1.
CP002684 Genomic DNA. Translation: AEE29242.1.
AY058194 mRNA. Translation: AAL25607.1.
AY142002 mRNA. Translation: AAM98266.1.
PIRiH86282.
RefSeqiNP_172945.1. NM_101361.3.
UniGeneiAt.15032.

Genome annotation databases

EnsemblPlantsiAT1G14920.1; AT1G14920.1; AT1G14920.
GeneIDi838057.
GrameneiAT1G14920.1; AT1G14920.1; AT1G14920.
KEGGiath:AT1G14920.

Similar proteinsi

Entry informationi

Entry nameiGAI_ARATH
AccessioniPrimary (citable) accession number: Q9LQT8
Secondary accession number(s): O23643, O23724
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families