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Q9LQQ9 (MPK13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 13

Short name=AtMPK13
Short name=MAP kinase 13
EC=2.7.11.24
Gene names
Name:MPK13
Ordered Locus Names:At1g07880
ORF Names:F24B9.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

MKK6-MPK13 module positively regulates lateral root formation. Ref.10

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated by the MAP kinase kinase MKK6 in vitro. Ref.6 Ref.8

Subunit structure

Interacts with MKK6. Ref.9

Tissue specificity

Expressed in roots, stems and flower buds. Ref.6

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Disruption phenotype

RNAi MPK13 displays fewer lateral roots. Ref.10

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MKK2Q9S7U92EBI-2358762,EBI-994350

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9LQQ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9LQQ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-254: LLGSPD → VSKLKP
     255-363: Missing.
Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Mitogen-activated protein kinase 13
PRO_0000245813

Regions

Domain33 – 319287Protein kinase
Nucleotide binding39 – 479ATP By similarity
Motif191 – 1933TXY

Sites

Active site1591Proton acceptor By similarity
Binding site621ATP By similarity

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity
Modified residue1961Phosphothreonine By similarity

Natural variations

Alternative sequence249 – 2546LLGSPD → VSKLKP in isoform 2.
VSP_036334
Alternative sequence255 – 363109Missing in isoform 2.
VSP_036335

Experimental info

Isoform 2:
Sequence conflict2431L → P in BX818168. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 88472CBA140DB739

FASTA36342,195
        10         20         30         40         50         60 
MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA TNSETNEEVA 

        70         80         90        100        110        120 
IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE LPEKERFEDV YIVYELMDTD 

       130        140        150        160        170        180 
LHQIIRSTQT LTDDHCQYFL YQILRGLKYI HSANVLHRDL KPSNLVLNTN CDLKICDFGL 

       190        200        210        220        230        240 
ARTSNETEIM TEYVVTRWYR APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV 

       250        260        270        280        290        300 
QQLKLITELL GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML 

       310        320        330        340        350        360 
VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK ELVWRESLHF 


KNM 

« Hide

Isoform 2 [UniParc].

Checksum: C4B014E91DA203A4
Show »

FASTA25429,454

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13."
Melikant B., Giuliani C., Halbmayer-Watzina S., Limmongkon A., Heberle-Bors E., Wilson C.
FEBS Lett. 576:5-8(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, TISSUE SPECIFICITY.
[7]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[8]"The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana."
Kosetsu K., Matsunaga S., Nakagami H., Colcombet J., Sasabe M., Soyano T., Takahashi Y., Hirt H., Machida Y.
Plant Cell 22:3778-3790(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[9]"AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
Zeng Q., Chen J.G., Ellis B.E.
Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK6.
[10]"AtMKK6 and AtMPK13 are required for lateral root formation in Arabidopsis."
Zeng Q., Sritubtim S., Ellis B.E.
Plant Signal. Behav. 6:1436-1439(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007583 Genomic DNA. Translation: AAF75067.1.
CP002684 Genomic DNA. Translation: AEE28197.1.
CP002684 Genomic DNA. Translation: AEE28198.1.
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1.
PIRC86214.
RefSeqNP_001030990.1. NM_001035913.1. [Q9LQQ9-1]
NP_172266.2. NM_100662.3. [Q9LQQ9-2]
UniGeneAt.49865.

3D structure databases

ProteinModelPortalQ9LQQ9.
SMRQ9LQQ9. Positions 8-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22544. 1 interaction.
IntActQ9LQQ9. 2 interactions.
MINTMINT-1206087.

Proteomic databases

PRIDEQ9LQQ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G07880.2; AT1G07880.2; AT1G07880. [Q9LQQ9-1]
GeneID837303.
KEGGath:AT1G07880.

Organism-specific databases

GeneFarm843. 89.
TAIRAT1G07880.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9LQQ9.
KOK04371.
OMANSETNEE.
PhylomeDBQ9LQQ9.

Enzyme and pathway databases

BioCycARA:AT1G07880-MONOMER.
ARA:GQT-2615-MONOMER.

Gene expression databases

GenevestigatorQ9LQQ9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK13_ARATH
AccessionPrimary (citable) accession number: Q9LQQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names