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Protein

Mitogen-activated protein kinase 13

Gene

MPK13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK6-MPK13 module positively regulates lateral root formation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated by the MAP kinase kinase MKK6 in vitro.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ATPPROSITE-ProRule annotation1
Active sitei159Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 47ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • lateral root formation Source: UniProtKB
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-375165. NCAM signaling for neurite out-growth.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-444257. RSK activation.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-881907. Gastrin-CREB signalling pathway via PKC and MAPK.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 13 (EC:2.7.11.24)
Short name:
AtMPK13
Short name:
MAP kinase 13
Gene namesi
Name:MPK13
Ordered Locus Names:At1g07880
ORF Names:F24B9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G07880.
TAIRilocus:2026484. AT1G07880.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

RNAi MPK13 displays fewer lateral roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458131 – 363Mitogen-activated protein kinase 13Add BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1
Modified residuei196PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LQQ9.
PRIDEiQ9LQQ9.

PTM databases

iPTMnetiQ9LQQ9.

Expressioni

Tissue specificityi

Expressed in roots, stems and flower buds.1 Publication

Gene expression databases

ExpressionAtlasiQ9LQQ9. baseline and differential.
GenevisibleiQ9LQQ9. AT.

Interactioni

Subunit structurei

Interacts with MKK6.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi22544. 2 interactors.
IntActiQ9LQQ9. 2 interactors.
MINTiMINT-1206087.
STRINGi3702.AT1G07880.2.

Structurei

3D structure databases

ProteinModelPortaliQ9LQQ9.
SMRiQ9LQQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LQQ9.
KOiK04371.
OMAiYLIQEYM.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ9LQQ9.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LQQ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA
60 70 80 90 100
TNSETNEEVA IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE
110 120 130 140 150
LPEKERFEDV YIVYELMDTD LHQIIRSTQT LTDDHCQYFL YQILRGLKYI
160 170 180 190 200
HSANVLHRDL KPSNLVLNTN CDLKICDFGL ARTSNETEIM TEYVVTRWYR
210 220 230 240 250
APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV QQLKLITELL
260 270 280 290 300
GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML
310 320 330 340 350
VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK
360
ELVWRESLHF KNM
Length:363
Mass (Da):42,195
Last modified:October 1, 2000 - v1
Checksum:i88472CBA140DB739
GO
Isoform 2 (identifier: Q9LQQ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-254: LLGSPD → VSKLKP
     255-363: Missing.

Note: May be due to an intron retention. No experimental confirmation available.Curated
Show »
Length:254
Mass (Da):29,454
Checksum:iC4B014E91DA203A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q9LQQ9-2)
Sequence conflicti243L → P in BX818168 (PubMed:14993207).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036334249 – 254LLGSPD → VSKLKP in isoform 2. 1 Publication6
Alternative sequenceiVSP_036335255 – 363Missing in isoform 2. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA. Translation: AAF75067.1.
CP002684 Genomic DNA. Translation: AEE28197.1.
CP002684 Genomic DNA. Translation: AEE28198.1.
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1.
PIRiC86214.
RefSeqiNP_001030990.1. NM_001035913.2. [Q9LQQ9-1]
NP_172266.2. NM_100662.4. [Q9LQQ9-2]
UniGeneiAt.49865.

Genome annotation databases

EnsemblPlantsiAT1G07880.1; AT1G07880.1; AT1G07880. [Q9LQQ9-2]
AT1G07880.2; AT1G07880.2; AT1G07880. [Q9LQQ9-1]
GeneIDi837303.
GrameneiAT1G07880.1; AT1G07880.1; AT1G07880.
AT1G07880.2; AT1G07880.2; AT1G07880.
KEGGiath:AT1G07880.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMPK13_ARATH
AccessioniPrimary (citable) accession number: Q9LQQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families