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Q9LQQ9

- MPK13_ARATH

UniProt

Q9LQQ9 - MPK13_ARATH

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Protein

Mitogen-activated protein kinase 13

Gene
MPK13, At1g07880, F24B9.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

MKK6-MPK13 module positively regulates lateral root formation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity. Activated by the MAP kinase kinase MKK6 in vitro.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ATP By similarity
Active sitei159 – 1591Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 479ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
  3. protein binding Source: IntAct

GO - Biological processi

  1. lateral root formation Source: UniProtKB
  2. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G07880-MONOMER.
ARA:GQT-2615-MONOMER.
ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 13 (EC:2.7.11.24)
Short name:
AtMPK13
Short name:
MAP kinase 13
Gene namesi
Name:MPK13
Ordered Locus Names:At1g07880
ORF Names:F24B9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G07880.

Pathology & Biotechi

Disruption phenotypei

RNAi MPK13 displays fewer lateral roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Mitogen-activated protein kinase 13PRO_0000245813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911Phosphothreonine By similarity
Modified residuei193 – 1931Phosphotyrosine By similarity
Modified residuei196 – 1961Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9LQQ9.

Expressioni

Tissue specificityi

Expressed in roots, stems and flower buds.1 Publication

Gene expression databases

GenevestigatoriQ9LQQ9.

Interactioni

Subunit structurei

Interacts with MKK6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK2Q9S7U92EBI-2358762,EBI-994350

Protein-protein interaction databases

BioGridi22544. 1 interaction.
IntActiQ9LQQ9. 2 interactions.
MINTiMINT-1206087.

Structurei

3D structure databases

ProteinModelPortaliQ9LQQ9.
SMRiQ9LQQ9. Positions 8-361.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 319287Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9LQQ9.
KOiK04371.
OMAiNSETNEE.
PhylomeDBiQ9LQQ9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9LQQ9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA    50
TNSETNEEVA IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE 100
LPEKERFEDV YIVYELMDTD LHQIIRSTQT LTDDHCQYFL YQILRGLKYI 150
HSANVLHRDL KPSNLVLNTN CDLKICDFGL ARTSNETEIM TEYVVTRWYR 200
APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV QQLKLITELL 250
GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML 300
VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK 350
ELVWRESLHF KNM 363
Length:363
Mass (Da):42,195
Last modified:October 1, 2000 - v1
Checksum:i88472CBA140DB739
GO
Isoform 2 (identifier: Q9LQQ9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-254: LLGSPD → VSKLKP
     255-363: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:254
Mass (Da):29,454
Checksum:iC4B014E91DA203A4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 2546LLGSPD → VSKLKP in isoform 2. VSP_036334
Alternative sequencei255 – 363109Missing in isoform 2. VSP_036335Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q9LQQ9-2)
Sequence conflicti243 – 2431L → P in BX818168. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007583 Genomic DNA. Translation: AAF75067.1.
CP002684 Genomic DNA. Translation: AEE28197.1.
CP002684 Genomic DNA. Translation: AEE28198.1.
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1.
PIRiC86214.
RefSeqiNP_001030990.1. NM_001035913.1. [Q9LQQ9-1]
NP_172266.2. NM_100662.3. [Q9LQQ9-2]
UniGeneiAt.49865.

Genome annotation databases

EnsemblPlantsiAT1G07880.2; AT1G07880.2; AT1G07880. [Q9LQQ9-1]
GeneIDi837303.
KEGGiath:AT1G07880.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007583 Genomic DNA. Translation: AAF75067.1 .
CP002684 Genomic DNA. Translation: AEE28197.1 .
CP002684 Genomic DNA. Translation: AEE28198.1 .
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1 .
PIRi C86214.
RefSeqi NP_001030990.1. NM_001035913.1. [Q9LQQ9-1 ]
NP_172266.2. NM_100662.3. [Q9LQQ9-2 ]
UniGenei At.49865.

3D structure databases

ProteinModelPortali Q9LQQ9.
SMRi Q9LQQ9. Positions 8-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22544. 1 interaction.
IntActi Q9LQQ9. 2 interactions.
MINTi MINT-1206087.

Proteomic databases

PRIDEi Q9LQQ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G07880.2 ; AT1G07880.2 ; AT1G07880 . [Q9LQQ9-1 ]
GeneIDi 837303.
KEGGi ath:AT1G07880.

Organism-specific databases

GeneFarmi 843. 89.
TAIRi AT1G07880.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q9LQQ9.
KOi K04371.
OMAi NSETNEE.
PhylomeDBi Q9LQQ9.

Enzyme and pathway databases

BioCyci ARA:AT1G07880-MONOMER.
ARA:GQT-2615-MONOMER.
Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
REACT_190946. KSRP destabilizes mRNA.
REACT_202229. ERK1 activation.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_216613. Signalling to ERK5.

Gene expression databases

Genevestigatori Q9LQQ9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13."
    Melikant B., Giuliani C., Halbmayer-Watzina S., Limmongkon A., Heberle-Bors E., Wilson C.
    FEBS Lett. 576:5-8(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, TISSUE SPECIFICITY.
  7. Cited for: GENE FAMILY.
  8. "The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana."
    Kosetsu K., Matsunaga S., Nakagami H., Colcombet J., Sasabe M., Soyano T., Takahashi Y., Hirt H., Machida Y.
    Plant Cell 22:3778-3790(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
    Zeng Q., Chen J.G., Ellis B.E.
    Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK6.
  10. "AtMKK6 and AtMPK13 are required for lateral root formation in Arabidopsis."
    Zeng Q., Sritubtim S., Ellis B.E.
    Plant Signal. Behav. 6:1436-1439(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMPK13_ARATH
AccessioniPrimary (citable) accession number: Q9LQQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi