Q9LQQ9 (MPK13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase 13
Short name=AtMPK13
Short name=MAP kinase 13
EC=2.7.11.24

Gene names

Name:	MPK13
Ordered Locus Names:	At1g07880
ORF Names:	F24B9.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity. Activated by the MAP kinase kinase MKK6 in vitro. Ref.6
Tissue specificity	Expressed in roots, stems and flower buds. Ref.6
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from sequence or structural similarity PubMed 11544109. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MKK2	Q9S7U9	2	EBI-2358762,EBI-994350

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LQQ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LQQ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-254: LLGSPD → VSKLKP
     255-363: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Mitogen-activated protein kinase 13		PRO_0000245813
Regions
	Domain	33 – 319	287	Protein kinase
	Nucleotide binding	39 – 47	9	ATP By similarity
	Motif	191 – 193	3	TXY
Sites
	Active site	159	1	Proton acceptor By similarity
	Binding site	62	1	ATP By similarity
Amino acid modifications
	Modified residue	191	1	Phosphothreonine By similarity
	Modified residue	193	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	249 – 254	6	LLGSPD → VSKLKP in isoform 2.		VSP_036334
	Alternative sequence	255 – 363	109	Missing in isoform 2.		VSP_036335
Experimental info
Isoform 2:
	Sequence conflict	243	1	L → P in BX818168. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 88472CBA140DB739	FASTA	363	42,195
	10 20 30 40 50 60 MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA TNSETNEEVA 70 80 90 100 110 120 IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE LPEKERFEDV YIVYELMDTD 130 140 150 160 170 180 LHQIIRSTQT LTDDHCQYFL YQILRGLKYI HSANVLHRDL KPSNLVLNTN CDLKICDFGL 190 200 210 220 230 240 ARTSNETEIM TEYVVTRWYR APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV 250 260 270 280 290 300 QQLKLITELL GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML 310 320 330 340 350 360 VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK ELVWRESLHF KNM
	Isoform 2 [UniParc]. Checksum: C4B014E91DA203A4	FASTA	254	29,454
	10 20 30 40 50 60 MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA TNSETNEEVA 70 80 90 100 110 120 IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE LPEKERFEDV YIVYELMDTD 130 140 150 160 170 180 LHQIIRSTQT LTDDHCQYFL YQILRGLKYI HSANVLHRDL KPSNLVLNTN CDLKICDFGL 190 200 210 220 230 240 ARTSNETEIM TEYVVTRWYR APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV 250 QQLKLITEVS KLKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[6]	"The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13." Melikant B., Giuliani C., Halbmayer-Watzina S., Limmongkon A., Heberle-Bors E., Wilson C. FEBS Lett. 576:5-8(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007583 Genomic DNA. Translation: AAF75067.1. CP002684 Genomic DNA. Translation: AEE28197.1. CP002684 Genomic DNA. Translation: AEE28198.1. BX818168 mRNA. No translation available. BT015822 mRNA. Translation: AAU94385.1.
IPI	IPI00519646. IPI00657259.
PIR	C86214.
RefSeq	NP_001030990.1. NM_001035913.1. NP_172266.2. NM_100662.3.
UniGene	At.49865.
3D structure databases
ProteinModelPortal	Q9LQQ9.
SMR	Q9LQQ9. Positions 13-359.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LQQ9. 2 interactions.
MINT	MINT-1206087.
Proteomic databases
PRIDE	Q9LQQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G07880.2; AT1G07880.2; AT1G07880.
GeneID	837303.
KEGG	ath:AT1G07880.
Organism-specific databases
GeneFarm	843. 89.
TAIR	At1g07880.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q9LQQ9.
KO	K04371.
OMA	AYGIVCC.
PhylomeDB	Q9LQQ9.
ProtClustDB	CLSN2918687.
Gene expression databases
Genevestigator	Q9LQQ9.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPK13_ARATH

Accession

Primary (citable) accession number: Q9LQQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program