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Protein

Mitogen-activated protein kinase 13

Gene

MPK13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK6-MPK13 module positively regulates lateral root formation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated by the MAP kinase kinase MKK6 in vitro.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ATPPROSITE-ProRule annotation
Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 479ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. lateral root formation Source: UniProtKB
  2. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G07880-MONOMER.
ARA:GQT-2615-MONOMER.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 13 (EC:2.7.11.24)
Short name:
AtMPK13
Short name:
MAP kinase 13
Gene namesi
Name:MPK13
Ordered Locus Names:At1g07880
ORF Names:F24B9.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G07880.

Pathology & Biotechi

Disruption phenotypei

RNAi MPK13 displays fewer lateral roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Mitogen-activated protein kinase 13PRO_0000245813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei193 – 1931PhosphotyrosineBy similarity
Modified residuei196 – 1961PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9LQQ9.

Expressioni

Tissue specificityi

Expressed in roots, stems and flower buds.1 Publication

Gene expression databases

GenevestigatoriQ9LQQ9.

Interactioni

Subunit structurei

Interacts with MKK6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK2Q9S7U92EBI-2358762,EBI-994350

Protein-protein interaction databases

BioGridi22544. 1 interaction.
IntActiQ9LQQ9. 2 interactions.
MINTiMINT-1206087.

Structurei

3D structure databases

ProteinModelPortaliQ9LQQ9.
SMRiQ9LQQ9. Positions 36-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 319287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9LQQ9.
KOiK04371.
OMAiFSPKKRI.
PhylomeDBiQ9LQQ9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LQQ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKREDGGIL TYDGRYVMYN VLGNIFELSS KYIPPIEPIG RGAYGIVCCA
60 70 80 90 100
TNSETNEEVA IKKIANAFDN RVDAKRTLRE IKLLSHMDHD NVIKIKDIIE
110 120 130 140 150
LPEKERFEDV YIVYELMDTD LHQIIRSTQT LTDDHCQYFL YQILRGLKYI
160 170 180 190 200
HSANVLHRDL KPSNLVLNTN CDLKICDFGL ARTSNETEIM TEYVVTRWYR
210 220 230 240 250
APELLLNSSE YTGAIDIWSV GCIFMEILRR ETLFPGKDYV QQLKLITELL
260 270 280 290 300
GSPDDSDLDF LRSDNARKYV KQLPHVQKQS FREKFPNISP MALDLAEKML
310 320 330 340 350
VFDPSKRITV DEALKQPYLA SLHEINEEPT CPTPFSFDFE ETALDEQDIK
360
ELVWRESLHF KNM
Length:363
Mass (Da):42,195
Last modified:October 1, 2000 - v1
Checksum:i88472CBA140DB739
GO
Isoform 2 (identifier: Q9LQQ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-254: LLGSPD → VSKLKP
     255-363: Missing.

Note: May be due to an intron retention. No experimental confirmation available.Curated

Show »
Length:254
Mass (Da):29,454
Checksum:iC4B014E91DA203A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q9LQQ9-2)
Sequence conflicti243 – 2431L → P in BX818168 (PubMed:14993207).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 2546LLGSPD → VSKLKP in isoform 2. 1 PublicationVSP_036334
Alternative sequencei255 – 363109Missing in isoform 2. 1 PublicationVSP_036335Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA. Translation: AAF75067.1.
CP002684 Genomic DNA. Translation: AEE28197.1.
CP002684 Genomic DNA. Translation: AEE28198.1.
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1.
PIRiC86214.
RefSeqiNP_001030990.1. NM_001035913.1. [Q9LQQ9-1]
NP_172266.2. NM_100662.3. [Q9LQQ9-2]
UniGeneiAt.49865.

Genome annotation databases

EnsemblPlantsiAT1G07880.2; AT1G07880.2; AT1G07880. [Q9LQQ9-1]
GeneIDi837303.
KEGGiath:AT1G07880.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA. Translation: AAF75067.1.
CP002684 Genomic DNA. Translation: AEE28197.1.
CP002684 Genomic DNA. Translation: AEE28198.1.
BX818168 mRNA. No translation available.
BT015822 mRNA. Translation: AAU94385.1.
PIRiC86214.
RefSeqiNP_001030990.1. NM_001035913.1. [Q9LQQ9-1]
NP_172266.2. NM_100662.3. [Q9LQQ9-2]
UniGeneiAt.49865.

3D structure databases

ProteinModelPortaliQ9LQQ9.
SMRiQ9LQQ9. Positions 36-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22544. 1 interaction.
IntActiQ9LQQ9. 2 interactions.
MINTiMINT-1206087.

Proteomic databases

PRIDEiQ9LQQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07880.2; AT1G07880.2; AT1G07880. [Q9LQQ9-1]
GeneIDi837303.
KEGGiath:AT1G07880.

Organism-specific databases

GeneFarmi843. 89.
TAIRiAT1G07880.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9LQQ9.
KOiK04371.
OMAiFSPKKRI.
PhylomeDBiQ9LQQ9.

Enzyme and pathway databases

BioCyciARA:AT1G07880-MONOMER.
ARA:GQT-2615-MONOMER.
ReactomeiREACT_202229. ERK1 activation.
REACT_206508. ERK/MAPK targets.
REACT_208246. ERKs are inactivated.
REACT_209747. ERK2 activation.
REACT_220111. Regulation of HSF1-mediated heat shock response.
REACT_245123. Signal transduction by L1.
REACT_247278. ISG15 antiviral mechanism.
REACT_251578. Signaling by FGFR.
REACT_268338. Signalling to ERK5.

Gene expression databases

GenevestigatoriQ9LQQ9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "The Arabidopsis thaliana MEK AtMKK6 activates the MAP kinase AtMPK13."
    Melikant B., Giuliani C., Halbmayer-Watzina S., Limmongkon A., Heberle-Bors E., Wilson C.
    FEBS Lett. 576:5-8(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, TISSUE SPECIFICITY.
  7. Cited for: GENE FAMILY.
  8. "The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana."
    Kosetsu K., Matsunaga S., Nakagami H., Colcombet J., Sasabe M., Soyano T., Takahashi Y., Hirt H., Machida Y.
    Plant Cell 22:3778-3790(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
    Zeng Q., Chen J.G., Ellis B.E.
    Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK6.
  10. "AtMKK6 and AtMPK13 are required for lateral root formation in Arabidopsis."
    Zeng Q., Sritubtim S., Ellis B.E.
    Plant Signal. Behav. 6:1436-1439(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMPK13_ARATH
AccessioniPrimary (citable) accession number: Q9LQQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.