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Protein

Protein NRT1/ PTR FAMILY 7.3

Gene

NPF7.3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent bidirectional nitrate transporter. Involved in nitrate loading into xylem and not in nitrate uptake. Not involved in histidine or dipeptides transport.1 Publication

Kineticsi

  1. KM=6 mM for nitrate1 Publication

    GO - Molecular functioni

    • nitrate transmembrane transporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • leaf senescence Source: TAIR
    • nitrate transport Source: TAIR
    • potassium ion homeostasis Source: TAIR
    • response to nitrate Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Nitrate assimilation, Symport, Transport

    Protein family/group databases

    TCDBi2.A.17.3.19. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 7.3
    Short name:
    AtNPF7.3
    Alternative name(s):
    Nitrate transporter 1.5
    Gene namesi
    Name:NPF7.3
    Synonyms:NRT1.5
    Ordered Locus Names:At1g32450
    ORF Names:F5D14.23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G32450.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
    Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
    Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
    Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
    Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
    Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
    Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
    Transmembranei515 – 535HelicalSequence analysisAdd BLAST21
    Transmembranei559 – 579HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype when grown under normal conditions. Lower nitrate concentration in xylem sap. Decreased long-distance root-to-shoot transport of nitrate but not of sulfate or phosphate.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999481 – 614Protein NRT1/ PTR FAMILY 7.3Add BLAST614

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei111PhosphothreonineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LQL2.

    Expressioni

    Tissue specificityi

    High expression in roots. Barely detected in shoots. Expressed in root pericycle cells close to the xylem.3 Publications

    Inductioni

    Up-regulated slowly by nitrate. Down-regulated by cadmium and high pH. Circadian-regulation. Expression increase during the dark phase and decrease during the light phase.2 Publications

    Gene expression databases

    GenevisibleiQ9LQL2. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi25373. 1 interactor.
    STRINGi3702.AT1G32450.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LQL2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ9LQL2.
    OMAiWRLCPIS.
    OrthoDBiEOG093605FC.
    PhylomeDBiQ9LQL2.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9LQL2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCLEIYNKD TMKKKEGEEE TRDGTVDYYG RPSIRSNSGQ WVAGIVILLN
    60 70 80 90 100
    QGLATLAFFG VGVNLVLFLT RVLQQNNADA ANNVSKWTGT VYIFSLVGAF
    110 120 130 140 150
    LSDSYWGRYK TCAIFQVIFV IGLSSLSLSS YMFLIRPRGC GDEVTPCGSH
    160 170 180 190 200
    SMMEITMFYF SIYLIALGYG GYQPNIATLG ADQFDEEHPK EGYSKIAFFS
    210 220 230 240 250
    YFYLALNLGS LFSNTILGYF EDEGMWALGF WASTGSAIIG LILFLVGTPR
    260 270 280 290 300
    YRYFKPTGNP LSRFCQVLVA ATKKSSVEAP LRGREEMYDG DSEGKNASVN
    310 320 330 340 350
    TGRRIVHTDE FKFLDKAAYI TARDLDDKKQ DSVNPWRLCP VTQVEEVKCI
    360 370 380 390 400
    LRLMPIWLCT IIYSVVFTQM ASLFVEQGAA MNTSVSDFKI PPASMSSFDI
    410 420 430 440 450
    LSVALFIFLY RRVLEPVANR FKKNGSKGIT ELHRMGIGLV IAVIAMIAAG
    460 470 480 490 500
    IVECYRLKYA DKSCTHCDGS SSLSIFWQAP QYSLIGASEV FMYVGQLEFF
    510 520 530 540 550
    NAQTPDGLKS FGSALCMMSM SMGNFVSSLL VTMVVKISTE DHMPGWIPRN
    560 570 580 590 600
    LNKGHLDRFY FLLAALTSID LVVYIACAKW YKPIQLEGKD EMQDMSDDDY
    610
    DTESEEEREK DSKV
    Length:614
    Mass (Da):68,699
    Last modified:November 2, 2010 - v2
    Checksum:i19D9C8FB1E975DFD
    GO

    Sequence cautioni

    The sequence AAF81343 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC007767 Genomic DNA. Translation: AAF81343.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31488.1.
    BX841204 mRNA. No translation available.
    PIRiG86449.
    RefSeqiNP_174523.2. NM_102980.4.
    UniGeneiAt.43846.

    Genome annotation databases

    EnsemblPlantsiAT1G32450.1; AT1G32450.1; AT1G32450.
    GeneIDi840139.
    GrameneiAT1G32450.1; AT1G32450.1; AT1G32450.
    KEGGiath:AT1G32450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC007767 Genomic DNA. Translation: AAF81343.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE31488.1.
    BX841204 mRNA. No translation available.
    PIRiG86449.
    RefSeqiNP_174523.2. NM_102980.4.
    UniGeneiAt.43846.

    3D structure databases

    ProteinModelPortaliQ9LQL2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi25373. 1 interactor.
    STRINGi3702.AT1G32450.1.

    Protein family/group databases

    TCDBi2.A.17.3.19. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiQ9LQL2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G32450.1; AT1G32450.1; AT1G32450.
    GeneIDi840139.
    GrameneiAT1G32450.1; AT1G32450.1; AT1G32450.
    KEGGiath:AT1G32450.

    Organism-specific databases

    TAIRiAT1G32450.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiQ9LQL2.
    OMAiWRLCPIS.
    OrthoDBiEOG093605FC.
    PhylomeDBiQ9LQL2.

    Miscellaneous databases

    PROiQ9LQL2.

    Gene expression databases

    GenevisibleiQ9LQL2. AT.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTR14_ARATH
    AccessioniPrimary (citable) accession number: Q9LQL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: November 2, 2010
    Last modified: November 30, 2016
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.