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Protein

Protein NRT1/ PTR FAMILY 7.3

Gene

NPF7.3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low-affinity proton-dependent bidirectional nitrate transporter. Involved in nitrate loading into xylem and not in nitrate uptake. Not involved in histidine or dipeptides transport.1 Publication

Kineticsi

  1. KM=6 mM for nitrate1 Publication

    GO - Molecular functioni

    • nitrate transmembrane transporter activity Source: TAIR
    • potassium:proton antiporter activity Source: TAIR
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • leaf senescence Source: TAIR
    • nitrate transport Source: TAIR
    • potassium ion homeostasis Source: TAIR
    • proton transmembrane transport Source: TAIR
    • response to nitrate Source: TAIR

    Keywordsi

    Biological processNitrate assimilation, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.19 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 7.3
    Short name:
    AtNPF7.3
    Alternative name(s):
    Nitrate transporter 1.5
    Gene namesi
    Name:NPF7.3
    Synonyms:NRT1.5
    Ordered Locus Names:At1g32450
    ORF Names:F5D14.23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G32450
    TAIRilocus:2033776 AT1G32450

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
    Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
    Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
    Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
    Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
    Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
    Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
    Transmembranei515 – 535HelicalSequence analysisAdd BLAST21
    Transmembranei559 – 579HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype when grown under normal conditions. Lower nitrate concentration in xylem sap. Decreased long-distance root-to-shoot transport of nitrate but not of sulfate or phosphate.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999481 – 614Protein NRT1/ PTR FAMILY 7.3Add BLAST614

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei111PhosphothreonineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LQL2
    PRIDEiQ9LQL2

    Expressioni

    Tissue specificityi

    High expression in roots. Barely detected in shoots. Expressed in root pericycle cells close to the xylem.3 Publications

    Inductioni

    Up-regulated slowly by nitrate. Down-regulated by cadmium and high pH. Circadian-regulation. Expression increase during the dark phase and decrease during the light phase.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9LQL2 baseline and differential
    GenevisibleiQ9LQL2 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi25373, 1 interactor
    STRINGi3702.AT1G32450.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LQL2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237400
    InParanoidiQ9LQL2
    OMAiCTHCEGS
    OrthoDBiEOG093605FC
    PhylomeDBiQ9LQL2

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q9LQL2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCLEIYNKD TMKKKEGEEE TRDGTVDYYG RPSIRSNSGQ WVAGIVILLN
    60 70 80 90 100
    QGLATLAFFG VGVNLVLFLT RVLQQNNADA ANNVSKWTGT VYIFSLVGAF
    110 120 130 140 150
    LSDSYWGRYK TCAIFQVIFV IGLSSLSLSS YMFLIRPRGC GDEVTPCGSH
    160 170 180 190 200
    SMMEITMFYF SIYLIALGYG GYQPNIATLG ADQFDEEHPK EGYSKIAFFS
    210 220 230 240 250
    YFYLALNLGS LFSNTILGYF EDEGMWALGF WASTGSAIIG LILFLVGTPR
    260 270 280 290 300
    YRYFKPTGNP LSRFCQVLVA ATKKSSVEAP LRGREEMYDG DSEGKNASVN
    310 320 330 340 350
    TGRRIVHTDE FKFLDKAAYI TARDLDDKKQ DSVNPWRLCP VTQVEEVKCI
    360 370 380 390 400
    LRLMPIWLCT IIYSVVFTQM ASLFVEQGAA MNTSVSDFKI PPASMSSFDI
    410 420 430 440 450
    LSVALFIFLY RRVLEPVANR FKKNGSKGIT ELHRMGIGLV IAVIAMIAAG
    460 470 480 490 500
    IVECYRLKYA DKSCTHCDGS SSLSIFWQAP QYSLIGASEV FMYVGQLEFF
    510 520 530 540 550
    NAQTPDGLKS FGSALCMMSM SMGNFVSSLL VTMVVKISTE DHMPGWIPRN
    560 570 580 590 600
    LNKGHLDRFY FLLAALTSID LVVYIACAKW YKPIQLEGKD EMQDMSDDDY
    610
    DTESEEEREK DSKV
    Length:614
    Mass (Da):68,699
    Last modified:November 2, 2010 - v2
    Checksum:i19D9C8FB1E975DFD
    GO

    Sequence cautioni

    The sequence AAF81343 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC007767 Genomic DNA Translation: AAF81343.1 Sequence problems.
    CP002684 Genomic DNA Translation: AEE31488.1
    BX841204 mRNA No translation available.
    PIRiG86449
    RefSeqiNP_174523.2, NM_102980.4
    UniGeneiAt.43846

    Genome annotation databases

    EnsemblPlantsiAT1G32450.1; AT1G32450.1; AT1G32450
    GeneIDi840139
    GrameneiAT1G32450.1; AT1G32450.1; AT1G32450
    KEGGiath:AT1G32450

    Similar proteinsi

    Entry informationi

    Entry nameiPTR14_ARATH
    AccessioniPrimary (citable) accession number: Q9LQL2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: November 2, 2010
    Last modified: May 23, 2018
    This is version 87 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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