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Protein

Glycine cleavage system H protein 3, mitochondrial

Gene

GDH3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The glycine decarboxylase (GDC) or glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).By similarity

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

Enzyme regulationi

Inhibited by harpin, S-nitrosoglutathione (GSNO), nitric oxide, N-ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoic acid).1 Publication

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein 3, mitochondrial
Gene namesi
Name:GDH3
Ordered Locus Names:At1g32470
ORF Names:F5D14.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G32470.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • glycine cleavage complex Source: InterPro
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionBy similarityAdd BLAST35
ChainiPRO_000001073036 – 166Glycine cleavage system H protein 3, mitochondrialAdd BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei141PhosphoserineBy similarity1

Post-translational modificationi

S-nitrosylated and/or glutathionylated at unknown positions in response to nitric oxide.1 Publication

Keywords - PTMi

Glutathionylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiQ9LQL0.
PRIDEiQ9LQL0.

PTM databases

iPTMnetiQ9LQL0.

Expressioni

Gene expression databases

GenevisibleiQ9LQL0. AT.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi25375. 1 interactor.
STRINGi3702.AT1G32470.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LQL0.
SMRiQ9LQL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 139Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST83

Sequence similaritiesi

Belongs to the GcvH family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
HOGENOMiHOG000239392.
InParanoidiQ9LQL0.
KOiK02437.
OMAiIKMTIEK.
OrthoDBiEOG09360PSV.
PhylomeDBiQ9LQL0.

Family and domain databases

CDDicd06848. GCS_H. 1 hit.
HAMAPiMF_00272. GcvH. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR033753. GCV_H/Fam206.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LQL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRMWASST ANALKLSSSA SKSHLLPAFS ISRCFSSVLE GLKYANSHEW
60 70 80 90 100
VKHEGSVATI GITDHAQDHL GEVVFVELPE ANSSVSKEKS FGAVESVKAT
110 120 130 140 150
SEILSPISGE VIEVNTKLTE SPGLINSSPY EDGWMIKVKP SSPAELEALM
160
GPKEYTKFCE EEDAAH
Length:166
Mass (Da):17,897
Last modified:October 1, 2000 - v1
Checksum:iC21658C0B9E75ADB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81345.1.
CP002684 Genomic DNA. Translation: AEE31490.1.
AF332465 mRNA. Translation: AAG48828.1.
AF385740 mRNA. Translation: AAK60330.1.
AY078028 mRNA. Translation: AAL77729.1.
PIRiA86450.
RefSeqiNP_174525.1. NM_102982.4.
UniGeneiAt.15531.
At.22333.
At.69604.

Genome annotation databases

EnsemblPlantsiAT1G32470.1; AT1G32470.1; AT1G32470.
GeneIDi840141.
GrameneiAT1G32470.1; AT1G32470.1; AT1G32470.
KEGGiath:AT1G32470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007767 Genomic DNA. Translation: AAF81345.1.
CP002684 Genomic DNA. Translation: AEE31490.1.
AF332465 mRNA. Translation: AAG48828.1.
AF385740 mRNA. Translation: AAK60330.1.
AY078028 mRNA. Translation: AAL77729.1.
PIRiA86450.
RefSeqiNP_174525.1. NM_102982.4.
UniGeneiAt.15531.
At.22333.
At.69604.

3D structure databases

ProteinModelPortaliQ9LQL0.
SMRiQ9LQL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25375. 1 interactor.
STRINGi3702.AT1G32470.1.

PTM databases

iPTMnetiQ9LQL0.

Proteomic databases

PaxDbiQ9LQL0.
PRIDEiQ9LQL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G32470.1; AT1G32470.1; AT1G32470.
GeneIDi840141.
GrameneiAT1G32470.1; AT1G32470.1; AT1G32470.
KEGGiath:AT1G32470.

Organism-specific databases

TAIRiAT1G32470.

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
HOGENOMiHOG000239392.
InParanoidiQ9LQL0.
KOiK02437.
OMAiIKMTIEK.
OrthoDBiEOG09360PSV.
PhylomeDBiQ9LQL0.

Enzyme and pathway databases

ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-6783984. Glycine degradation.

Miscellaneous databases

PROiQ9LQL0.

Gene expression databases

GenevisibleiQ9LQL0. AT.

Family and domain databases

CDDicd06848. GCS_H. 1 hit.
HAMAPiMF_00272. GcvH. 1 hit.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR033753. GCV_H/Fam206.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCSH3_ARATH
AccessioniPrimary (citable) accession number: Q9LQL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was wrongly referred as Gly deshydrogenase subunit P2 in PubMed:20089767.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.