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Protein

Alkaline/neutral invertase CINV1

Gene

CINV1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytosolic invertase that specifically cleaves sucrose into glucose and fructose and is involved in the regulation of multiple tissue development including primary root elongation, root hair growth, leaf and silique development, and floral transition. Is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells.4 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.1 Publication

Kineticsi

  1. KM=18.4 mM for sucrose2 Publications
  2. KM=8.4 mM for sucrose1 Publication

    pH dependencei

    Optimum pH is 7.0-8.0.3 Publications

    GO - Molecular functioni

    • beta-fructofuranosidase activity Source: TAIR
    • glycopeptide alpha-N-acetylgalactosaminidase activity Source: InterPro
    • sucrose alpha-glucosidase activity Source: TAIR

    GO - Biological processi

    • carbohydrate metabolic process Source: TAIR
    • cellular amino acid metabolic process Source: TAIR
    • response to hydrogen peroxide Source: TAIR
    • root development Source: TAIR
    • sucrose catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Protein family/group databases

    CAZyiGH100. Glycoside Hydrolase Family 100.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline/neutral invertase CINV1Curated (EC:3.2.1.261 Publication)
    Alternative name(s):
    Alkaline/neutral invertase GCurated
    Short name:
    A/N-INVG1 Publication
    Cytosolic invertase 11 Publication
    Short name:
    AtCYT-INV11 Publication
    Gene namesi
    Name:CINV11 Publication
    Synonyms:INVG1 Publication
    Ordered Locus Names:At1g35580
    ORF Names:F15O4.33
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G35580.

    Subcellular locationi

    • Cytoplasmcytosol 1 Publication
    • Nucleus 1 Publication

    • Note: Detected in membrane and nucleus when associated with PIP5K9.1 Publication

    GO - Cellular componenti

    • cytosol Source: TAIR
    • membrane Source: TAIR
    • nucleus Source: TAIR
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Reduced primary root length and increased length and number of lateral roots. Reduced plant growth and early flowering. Reduced seedling levels of glucose and fructose, but increased levels of sucrose.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Alkaline/neutral invertase CINV1PRO_0000422134Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineCombined sources
    Modified residuei11 – 111PhosphoserineCombined sources
    Modified residuei14 – 141PhosphoserineCombined sources
    Modified residuei44 – 441PhosphoserineCombined sources
    Modified residuei61 – 611PhosphoserineCombined sources
    Modified residuei70 – 701PhosphothreonineCombined sources
    Modified residuei547 – 5471PhosphoserineCombined sources

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9LQF2.
    PRIDEiQ9LQF2.

    PTM databases

    iPTMnetiQ9LQF2.

    Expressioni

    Tissue specificityi

    Expressed in radicle, hypocotyls, root tips and vascular cylinder, leaf vasculature, shoot stipules, trichomes, stem, stigma apex and base of siliques.1 Publication

    Inductioni

    Induced by mannitol in roots (PubMed:17508130). Induced by hydrogen peroxide (PubMed:21441406).2 Publications

    Gene expression databases

    ExpressionAtlasiQ9LQF2. baseline and differential.
    GenevisibleiQ9LQF2. AT.

    Interactioni

    Subunit structurei

    Interacts with PIP5K9. Interaction with PIP5K9 represses CINV1 activity.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PIP5K9Q8L8506EBI-2008033,EBI-2008013

    Protein-protein interaction databases

    BioGridi25686. 11 interactions.
    IntActiQ9LQF2. 3 interactions.
    STRINGi3702.AT1G35580.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LQF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 100 family.Curated

    Phylogenomic databases

    eggNOGiENOG410IVHI. Eukaryota.
    ENOG410XPYN. LUCA.
    HOGENOMiHOG000232531.
    InParanoidiQ9LQF2.
    OMAiIRETDNI.
    PhylomeDBiQ9LQF2.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR024746. Glyco_hydro_100.
    [Graphical view]
    PfamiPF12899. Glyco_hydro_100. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9LQF2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEGVGLRAVG SHCSLSEMDD LDLTRALDKP RLKIERKRSF DERSMSELST
    60 70 80 90 100
    GYSRHDGIHD SPRGRSVLDT PLSSARNSFE PHPMMAEAWE ALRRSMVFFR
    110 120 130 140 150
    GQPVGTLAAV DNTTDEVLNY DQVFVRDFVP SALAFLMNGE PDIVKHFLLK
    160 170 180 190 200
    TLQLQGWEKR VDRFKLGEGV MPASFKVLHD PIRETDNIVA DFGESAIGRV
    210 220 230 240 250
    APVDSGFWWI ILLRAYTKST GDLTLSETPE CQKGMKLILS LCLAEGFDTF
    260 270 280 290 300
    PTLLCADGCS MIDRRMGVYG YPIEIQALFF MALRSALSML KPDGDGREVI
    310 320 330 340 350
    ERIVKRLHAL SFHMRNYFWL DHQNLNDIYR FKTEEYSHTA VNKFNVMPDS
    360 370 380 390 400
    IPEWVFDFMP LRGGYFVGNV GPAHMDFRWF ALGNCVSILS SLATPDQSMA
    410 420 430 440 450
    IMDLLEHRWA ELVGEMPLKI CYPCLEGHEW RIVTGCDPKN TRWSYHNGGS
    460 470 480 490 500
    WPVLLWQLTA ACIKTGRPQI ARRAVDLIES RLHRDCWPEY YDGKLGRYVG
    510 520 530 540 550
    KQARKYQTWS IAGYLVAKML LEDPSHIGMI SLEEDKLMKP VIKRSASWPQ

    L
    Length:551
    Mass (Da):62,834
    Last modified:October 1, 2000 - v1
    Checksum:i8FE2EF4BC4DFC91F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AM230708 mRNA. Translation: CAJ76698.1.
    AC007887 Genomic DNA. Translation: AAF79356.1.
    CP002684 Genomic DNA. Translation: AEE31812.1.
    CP002684 Genomic DNA. Translation: AEE31813.1.
    AY065247 mRNA. Translation: AAL38723.1.
    AY142662 mRNA. Translation: AAN13200.1.
    PIRiA86477.
    RefSeqiNP_174791.2. NM_103255.2. [Q9LQF2-1]
    NP_849750.1. NM_179419.3. [Q9LQF2-1]
    UniGeneiAt.28624.
    At.74775.

    Genome annotation databases

    EnsemblPlantsiAT1G35580.1; AT1G35580.1; AT1G35580. [Q9LQF2-1]
    AT1G35580.2; AT1G35580.2; AT1G35580. [Q9LQF2-1]
    GeneIDi840454.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AM230708 mRNA. Translation: CAJ76698.1.
    AC007887 Genomic DNA. Translation: AAF79356.1.
    CP002684 Genomic DNA. Translation: AEE31812.1.
    CP002684 Genomic DNA. Translation: AEE31813.1.
    AY065247 mRNA. Translation: AAL38723.1.
    AY142662 mRNA. Translation: AAN13200.1.
    PIRiA86477.
    RefSeqiNP_174791.2. NM_103255.2. [Q9LQF2-1]
    NP_849750.1. NM_179419.3. [Q9LQF2-1]
    UniGeneiAt.28624.
    At.74775.

    3D structure databases

    ProteinModelPortaliQ9LQF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi25686. 11 interactions.
    IntActiQ9LQF2. 3 interactions.
    STRINGi3702.AT1G35580.1.

    Protein family/group databases

    CAZyiGH100. Glycoside Hydrolase Family 100.

    PTM databases

    iPTMnetiQ9LQF2.

    Proteomic databases

    PaxDbiQ9LQF2.
    PRIDEiQ9LQF2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G35580.1; AT1G35580.1; AT1G35580. [Q9LQF2-1]
    AT1G35580.2; AT1G35580.2; AT1G35580. [Q9LQF2-1]
    GeneIDi840454.

    Organism-specific databases

    TAIRiAT1G35580.

    Phylogenomic databases

    eggNOGiENOG410IVHI. Eukaryota.
    ENOG410XPYN. LUCA.
    HOGENOMiHOG000232531.
    InParanoidiQ9LQF2.
    OMAiIRETDNI.
    PhylomeDBiQ9LQF2.

    Miscellaneous databases

    PROiQ9LQF2.

    Gene expression databases

    ExpressionAtlasiQ9LQF2. baseline and differential.
    GenevisibleiQ9LQF2. AT.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR024746. Glyco_hydro_100.
    [Graphical view]
    PfamiPF12899. Glyco_hydro_100. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth."
      Lou Y., Gou J.Y., Xue H.W.
      Plant Cell 19:163-181(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH PIP5K9, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
      Tissue: Root.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis."
      Qi X., Wu Z., Li J., Mo X., Wu S., Chu J., Wu P.
      Plant Mol. Biol. 64:575-587(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION BY MANNITOL, DISRUPTION PHENOTYPE.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; SER-14; SER-61; THR-70 AND SER-547, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44; SER-61 AND THR-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: FUNCTION.
    10. "Exploring the neutral invertase-oxidative stress defence connection in Arabidopsis thaliana."
      Xiang L., Le Roy K., Bolouri-Moghaddam M.R., Vanhaecke M., Lammens W., Rolland F., Van den Ende W.
      J. Exp. Bot. 62:3849-3862(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION BY HYDROGEN PEROXIDE, GENE FAMILY, DISRUPTION PHENOTYPE.
    11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCINV1_ARATH
    AccessioniPrimary (citable) accession number: Q9LQF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: October 1, 2000
    Last modified: January 20, 2016
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.