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Q9LQC0 (HMOX4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heme oxygenase 4, chloroplastic

EC=1.14.99.3
Gene names
Name:HO4
Ordered Locus Names:At1g58300
ORF Names:F19C14.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Plays a minor role in phytochrome assembly and photomorphogenesis. Ref.6 Ref.7

Catalytic activity

Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

Subcellular location

Plastidchloroplast Ref.7.

Tissue specificity

Widely expressed at low levels. Ref.6

Disruption phenotype

No visible phenotype under normal growth conditions. Ref.6

Sequence similarities

Belongs to the heme oxygenase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.2. Ref.7

Sequence caution

The sequence ABK28442.1 differs from that shown. Reason: Erroneous termination at position 284. Translated as stop.

Ontologies

Keywords
   Biological processPhotosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processheme oxidation

Inferred from electronic annotation. Source: InterPro

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from direct assay PubMed 10072395. Source: TAIR

   Molecular_functionheme binding

Inferred from direct assay Ref.7. Source: TAIR

heme oxygenase (decyclizing) activity

Inferred from direct assay Ref.6Ref.7. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 283Heme oxygenase 4, chloroplasticPRO_0000412188

Sites

Metal binding911Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict92 – 943PRD → QEI in AAK63007. Ref.1
Sequence conflict1021D → V in AAK63007. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LQC0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: AB44816340D77DF5

FASTA28332,953
        10         20         30         40         50         60 
MATSRLNASC RFPASRRLDC ESYVSLRAKT VTIRYVRTIA APRRHLVRRA NEDQTLVVNV 

        70         80         90        100        110        120 
VAAAGEKPER RYPREPNGFV EEMRFVVMKI HPRDQVKEGK SDSNDLVSTW NFTIEGYLKF 

       130        140        150        160        170        180 
LVDSKLVFET LERIINESAI QAYAGLKNTG LERAENLSRD LEWFKEQGYE IPESMVPGKA 

       190        200        210        220        230        240 
YSQYLKNIAE KDPPAFICHF YNINFAHSAG GRMIGTKVAE KILDNKELEF YKWDGQLSEL 

       250        260        270        280 
LQNVSEELNK VAELWTREEK NHCLEETEKS FKFYWEIFRY LLS 

« Hide

References

« Hide 'large scale' references
[1]"The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants."
Davis S.J., Bhoo S.H., Durski A.M., Walker J.M., Vierstra R.D.
Plant Physiol. 126:656-669(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Regulation of HEMA1 expression by phytochrome and a plastid signal during de-etiolation in Arabidopsis thaliana."
McCormac A.C., Fischer A., Kumar A.M., Soll D., Terry M.J.
Plant J. 25:549-561(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 144-283.
[6]"Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis."
Emborg T.J., Walker J.M., Noh B., Vierstra R.D.
Plant Physiol. 140:856-868(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[7]"Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions."
Gisk B., Yasui Y., Kohchi T., Frankenberg-Dinkel N.
Biochem. J. 425:425-434(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF320023 Genomic DNA. Translation: AAK63007.1.
AC008051 Genomic DNA. Translation: AAF82257.1.
CP002684 Genomic DNA. Translation: AEE33533.1.
DQ446372 mRNA. Translation: ABE65721.1.
DQ652902 mRNA. Translation: ABK28442.1. Sequence problems.
AF295364 Genomic DNA. Translation: AAG30207.1.
PIRF96616.
RefSeqNP_176126.1. NM_104610.1.
UniGeneAt.52255.

3D structure databases

ProteinModelPortalQ9LQC0.
SMRQ9LQC0. Positions 78-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G58300.1-P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G58300.1; AT1G58300.1; AT1G58300.
GeneID842199.
KEGGath:AT1G58300.

Organism-specific databases

TAIRAT1G58300.

Phylogenomic databases

eggNOGNOG255302.
HOGENOMHOG000265822.
InParanoidQ9LQC0.
KOK00510.
OMAYNINFAH.
PhylomeDBQ9LQC0.

Enzyme and pathway databases

BioCycARA:AT1G58300-MONOMER.
MetaCyc:AT1G58300-MONOMER.

Gene expression databases

GenevestigatorQ9LQC0.

Family and domain databases

Gene3D1.20.910.10. 1 hit.
InterProIPR016053. Haem_Oase-like.
IPR016084. Haem_Oase-like_multi-hlx.
IPR016951. Haem_Oase_decyc_pln.
[Graphical view]
PfamPF01126. Heme_oxygenase. 1 hit.
[Graphical view]
PIRSFPIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAMSSF48613. SSF48613. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHMOX4_ARATH
AccessionPrimary (citable) accession number: Q9LQC0
Secondary accession number(s): A0MED2, Q94FX2, Q9FUN4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names