Q9LQC0 (HMOX4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme oxygenase 4, chloroplastic
EC=1.14.99.3

Gene names

Name:	HO4
Ordered Locus Names:	At1g58300
ORF Names:	F19C14.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Plays a minor role in phytochrome assembly and photomorphogenesis. Ref.6 Ref.7
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
Subcellular location	Plastid › chloroplast Ref.7.
Tissue specificity	Widely expressed at low levels. Ref.6
Disruption phenotype	No visible phenotype under normal growth conditions. Ref.6
Sequence similarities	Belongs to the heme oxygenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.2. Ref.7
Sequence caution	The sequence ABK28442.1 differs from that shown. Reason: Erroneous termination at position 284. Translated as stop.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme oxidation Inferred from electronic annotation. Source: InterPro photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from direct assay PubMed 10072395. Source: TAIR
Molecular_function	heme binding Inferred from direct assay Ref.7. Source: TAIR heme oxygenase (decyclizing) activity Inferred from direct assay Ref.6Ref.7. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 283		Heme oxygenase 4, chloroplastic		PRO_0000412188
Sites
	Metal binding	91	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	92 – 94	3	PRD → QEI in AAK63007. Ref.1
	Sequence conflict	102	1	D → V in AAK63007. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9LQC0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: AB44816340D77DF5	FASTA	283	32,953
	10 20 30 40 50 60 MATSRLNASC RFPASRRLDC ESYVSLRAKT VTIRYVRTIA APRRHLVRRA NEDQTLVVNV 70 80 90 100 110 120 VAAAGEKPER RYPREPNGFV EEMRFVVMKI HPRDQVKEGK SDSNDLVSTW NFTIEGYLKF 130 140 150 160 170 180 LVDSKLVFET LERIINESAI QAYAGLKNTG LERAENLSRD LEWFKEQGYE IPESMVPGKA 190 200 210 220 230 240 YSQYLKNIAE KDPPAFICHF YNINFAHSAG GRMIGTKVAE KILDNKELEF YKWDGQLSEL 250 260 270 280 LQNVSEELNK VAELWTREEK NHCLEETEKS FKFYWEIFRY LLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants." Davis S.J., Bhoo S.H., Durski A.M., Walker J.M., Vierstra R.D. Plant Physiol. 126:656-669(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Regulation of HEMA1 expression by phytochrome and a plastid signal during de-etiolation in Arabidopsis thaliana." McCormac A.C., Fischer A., Kumar A.M., Soll D., Terry M.J. Plant J. 25:549-561(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 144-283.
[6]	"Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis." Emborg T.J., Walker J.M., Noh B., Vierstra R.D. Plant Physiol. 140:856-868(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[7]	"Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions." Gisk B., Yasui Y., Kohchi T., Frankenberg-Dinkel N. Biochem. J. 425:425-434(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF320023 Genomic DNA. Translation: AAK63007.1. AC008051 Genomic DNA. Translation: AAF82257.1. CP002684 Genomic DNA. Translation: AEE33533.1. DQ446372 mRNA. Translation: ABE65721.1. DQ652902 mRNA. Translation: ABK28442.1. Sequence problems. AF295364 Genomic DNA. Translation: AAG30207.1.
IPI	IPI00537146.
PIR	F96616.
RefSeq	NP_176126.1. NM_104610.1.
UniGene	At.52255.
3D structure databases
ProteinModelPortal	Q9LQC0.
SMR	Q9LQC0. Positions 78-281.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G58300.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	842199.
KEGG	ath:AT1G58300.
Organism-specific databases
TAIR	At1g58300.
Phylogenomic databases
eggNOG	NOG255302.
HOGENOM	HOG000265822.
InParanoid	Q9LQC0.
KO	K00510.
OMA	CHFYNIN.
PhylomeDB	Q9LQC0.
ProtClustDB	CLSN2914675.
Gene expression databases
Genevestigator	Q9LQC0.
Family and domain databases
Gene3D	1.20.910.10. 1 hit.
InterPro	IPR016053. Haem_Oase-like. IPR016084. Haem_Oase-like_multi-hlx. IPR016951. Haem_Oase_decyc_pln. [Graphical view]
Pfam	PF01126. Heme_oxygenase. 1 hit. [Graphical view]
PIRSF	PIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HMOX4_ARATH

Accession

Primary (citable) accession number: Q9LQC0
Secondary accession number(s): A0MED2, Q94FX2, Q9FUN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program