Q9LQ55 (DRP2B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynamin-2B
EC=3.6.5.5

Alternative name(s):
Dynamin-like protein 3
Dynamin-related protein 2B

Gene names

Name:	DRP2B
Synonyms:	ADL3, CF1
Ordered Locus Names:	At1g59610
ORF Names:	T30E16.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative microtubule-associated force-producing protein, able to bind and hydrolyze GTP By similarity.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subcellular location	Cytoplasm › cytoskeleton Potential.
Tissue specificity	Ubiquitous. Preferentially expressed in siliques. Ref.1
Post-translational modification	Phosphorylation level varies significantly during early response to general elicitors.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.
Sequence caution	The sequence AAF79753.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA77516.1 differs from that shown. Reason: Frameshift at position 832. The sequence BAA88113.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	clathrin-mediated endocytosis Inferred from direct assay. Source: TAIR response to abscisic acid stimulus Inferred from direct assay. Source: TAIR
Cellular component	cell plate Inferred from direct assay. Source: TAIR clathrin-coated endocytic vesicle Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.7. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro clathrin binding Inferred from direct assay. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DRP1A	P42697	4	EBI-2355848,EBI-994234

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Dynamin-2B		PRO_0000206583
Regions
	Domain	579 – 703	125	PH
	Domain	737 – 830	94	GED
	Nucleotide binding	45 – 52	8	GTP Potential
	Nucleotide binding	143 – 147	5	GTP By similarity
	Nucleotide binding	204 – 207	4	GTP By similarity
	Coiled coil	788 – 812	25	Potential
	Compositional bias	885 – 920	36	Pro-rich
Amino acid modifications
	Modified residue	533	1	Phosphoserine Ref.9
	Modified residue	844	1	Phosphoserine Ref.7 Ref.8
	Modified residue	883	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q9LQ55 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: D95964FE33B79AB2	FASTA	920	100,228
	10 20 30 40 50 60 MEAIDELSQL SDSMRQAASL LADEDPDETS SSRRPATSLN VVALGNVGAG KSAVLNSLIG 70 80 90 100 110 120 HPVLPTGENG ATRAPIIIDL SREESLSSKA IILQIDNKNQ QVSASALRHS LQDRLSKGAS 130 140 150 160 170 180 GRGRDEIYLK LRTSTAPPLK LIDLPGLDQR IVDDSMIGEH AQHNDAILLV VVPASQASEI 190 200 210 220 230 240 SSSRALKIAK EYDPESTRTV GIISKIDQAA ENPKSLAAVQ ALLSNQGPPK TTDIPWVALI 250 260 270 280 290 300 GQSVSIASAQ SGGSENSLET AWRAESESLK SILTGAPQSK LGRIALVDTL ASQIRSRMKL 310 320 330 340 350 360 RLPNILTGLQ GKSQIVQDEL ARLGEQLVSS AEGTRAIALE LCREFEDKFL LHLAGGEGSG 370 380 390 400 410 420 WKVVASFEGN FPNRIKKLPL DRHFDLNNVK RIVLEADGYQ PYLISPEKGL RSLIKTVLEL 430 440 450 460 470 480 AKDPARLCVD EVHRVLVDIV SASANATPGL GRYPPFKREV VAIASAALDG FKNEAKKMVV 490 500 510 520 530 540 ALVDMERAFV PPQHFIRLVQ RRMERQRREE ELKGRSSKKG QDAEQSLLNR ATSPQPDGPS 550 560 570 580 590 600 STGGSLKSLR DKLMPQDKDK DKEKETPEVS GLKTAGPEGE ITAGYLMKKS AKTNGWSRRW 610 620 630 640 650 660 FVLNEKTGKL GYTKKQEERN FRGTVTLEEC SIEEISDDEG EKSKSSKDKK SNGPDSKGPG 670 680 690 700 710 720 LVFKITCRVP YKTVLKAHNA LVLKAESMVD KNEWINKLQK VIQARGGQVG SASMRQSLSE 730 740 750 760 770 780 GSLDKMVRKP VDPEEELRWM SQEVRGYVEA VLNSLAANVP KAVVLCQVEK SKEDMLNQLY 790 800 810 820 830 840 SSISAIGNER IESLIQEDQN VKRRRDRYQK QSSLLSKLTR QLSIHDNRAA AASSWSDNSG 850 860 870 880 890 900 TESSPRTNGG SSGEDWMNAF NAAASGPDSL KRYGSGGHSR RYSDPAQNGE DSSGSGGSSR 910 920 RTTPNRLPPA PPQSGSSYRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Arabidopsis thaliana dynamin-like protein containing the pleckstrin homology domain." Mikami K., Iuchi S., Yamaguchi-Shinozaki K., Shinozaki K. J. Exp. Bot. 51:317-318(2000) [PubMed: 10938838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1." Kato A., Suzuki M., Kuwahara A., Ooe H., Higano-Inaba K., Komeda Y. Gene 239:309-316(1999) [PubMed: 10548732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions." Hong Z., Bednarek S.Y., Blumwald E., Hwang I., Jurgens G., Menzel D., Osteryoung K.W., Raikhel N.V., Shinozaki K., Tsutsumi N., Verma D.P.S. Plant Mol. Biol. 53:261-265(2003) [PubMed: 14750516] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed: 17317660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-844, MASS SPECTROMETRY. Strain: cv. Columbia.
[8]	"Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses." Nuehse T.S., Bottrill A.R., Jones A.M.E., Peck S.C. Plant J. 51:931-940(2007) [PubMed: 17651370] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-844, MASS SPECTROMETRY.
[9]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-533 AND SER-883, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026987 mRNA. Translation: BAA77516.1. Frameshift. AB028467 mRNA. Translation: BAA88111.1. AB028469 Genomic DNA. Translation: BAA88113.1. Sequence problems. AC009317 Genomic DNA. Translation: AAF79753.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE33594.1. AY049230 mRNA. Translation: AAK83573.1. BT000772 mRNA. Translation: AAN31911.1. BT009695 mRNA. Translation: AAP88329.1.
IPI	IPI00544500.
PIR	H96619. T52426.
RefSeq	NP_176170.1. NM_104654.4.
UniGene	At.23927. At.67355.
3D structure databases
ProteinModelPortal	Q9LQ55.
SMR	Q9LQ55. Positions 4-354, 575-704.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-53383N.
IntAct	Q9LQ55. 4 interactions.
STRING	Q9LQ55.
Proteomic databases
PRIDE	Q9LQ55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G59610.1; AT1G59610.1; AT1G59610.
GeneID	842251.
GenomeReviews	Gene locus AT1G59610 in contig CT485782_GR.
KEGG	ath:AT1G59610.
NMPDR	fig|3702.1.peg.5343.
Organism-specific databases
GeneFarm	4741. 463.
TAIR	At1g59610.
Phylogenomic databases
eggNOG	KOG0446.
GeneTree	EPGT00070000028765.
HOGENOM	HBG593279.
InParanoid	Q9LQ55.
OMA	SSKKGHE.
PhylomeDB	Q9LQ55.
ProtClustDB	CLSN2679567.
Gene expression databases
ArrayExpress	Q9LQ55.
Genevestigator	Q9LQ55.
GermOnline	AT1G59610. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR022812. Dynamin. IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR020850. GTPase_effector_domain_GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRP2B_ARATH

Accession

Primary (citable) accession number: Q9LQ55
Secondary accession number(s): Q9SLT3, Q9SLT4, Q9SXX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program