Q9LQ48 (GSTUF_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U15
Short name=AtGSTU15
EC=2.5.1.18

Alternative name(s):
GST class-tau member 15

Gene names

Name:	GSTU15
Ordered Locus Names:	At1g59670
ORF Names:	T30E16.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence ABK28444.1 differs from that shown. Reason: Erroneous termination at position 234. Translated as stop.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.5. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.5. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Glutathione S-transferase U15		PRO_0000413561
Regions
	Domain	5 – 85	81	GST N-terminal
	Domain	92 – 219	128	GST C-terminal
	Region	15 – 16	2	Glutathione binding By similarity
	Region	42 – 43	2	Glutathione binding By similarity
	Region	56 – 57	2	Glutathione binding By similarity
	Region	69 – 70	2	Glutathione binding By similarity
Experimental info
	Sequence conflict	6	1	E → Q in AAM61551. Ref.4
	Sequence conflict	24	1	A → D in AAM61551. Ref.4
	Sequence conflict	38	1	D → N in AAM61551. Ref.4
	Sequence conflict	53 – 54	2	IF → VY in AAM61551. Ref.4
	Sequence conflict	141	1	E → D in AAM61551. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9LQ48 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8722CA2CE7AEC09D	FASTA	233	26,535
	10 20 30 40 50 60 MGEREEVKLL GTWYSPVVIR AKIALRLKSV DYDYVEEDLF GSKSELLLKS NPIFKKVPVL 70 80 90 100 110 120 IHNTKPVCVS LNIVEYIDET WNSSGSSILP SHPYDRALAR FWSVFVDDKW LPTLMAAVVA 130 140 150 160 170 180 KSEEAKAKGM EEVEEGLLQL EAAFIALSKG KSFFGGETIG FIDICLGSFL VLLKAREKLK 190 200 210 220 230 NEKILDELKT PSLYRWANQF LSNEMVKNVV PDIDKVAKFI EEFEDRAQYI RCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009317 Genomic DNA. Translation: AAF79758.1. CP002684 Genomic DNA. Translation: AEE33601.1. DQ446376 mRNA. Translation: ABE65725.1. DQ652904 mRNA. Translation: ABK28444.1. Sequence problems. AY084992 mRNA. Translation: AAM61551.1.
IPI	IPI00545485.
PIR	D96620.
RefSeq	NP_176176.1. NM_104660.2.
UniGene	At.36811.
3D structure databases
HSSP	HSSP built from PDB template 1GWC based on UniProtKB O04941.
ProteinModelPortal	Q9LQ48.
SMR	Q9LQ48. Positions 5-224.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G59670.1-P.
Proteomic databases
PRIDE	Q9LQ48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G59670.1; AT1G59670.1; AT1G59670.
GeneID	842257.
KEGG	ath:AT1G59670.
Organism-specific databases
TAIR	At1g59670.
Phylogenomic databases
eggNOG	NOG287605.
HOGENOM	HOG000125749.
InParanoid	Q9LQ48.
KO	K00799.
OMA	MAIWVEE.
PhylomeDB	Q9LQ48.
ProtClustDB	CLSN2679688.
Gene expression databases
ArrayExpress	Q9LQ48.
Genevestigator	Q9LQ48.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTUF_ARATH

Accession

Primary (citable) accession number: Q9LQ48
Secondary accession number(s): A0MED4, Q8LF83

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program