Reviewed, UniProtKB/Swiss-Prot Q9LPW7 (AFB3_ARATH)

Names and origin

Protein names

Recommended name:
    Protein AUXIN SIGNALING F-BOX 3

Gene names

Name:	AFB3
Ordered Locus Names:	At1g12820
ORF Names:	F13K23.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1 and SKP1A/ASK1. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner. Ref.4
Subcellular location	Nucleus. Ref.4
Tissue specificity	Ubiquitous, with higher levels in flowers. Ref.4
Induction	Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22). Ref.6
Domain	The F-box is necessary for the interaction with SKP1 By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Plant defense Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB defense response Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pollen maturation Inferred from genetic interaction. Source: TAIR response to molecule of bacterial origin Ref.6 Inferred from expression pattern. Source: TAIR stamen development Inferred from genetic interaction. Source: TAIR
Cellular component	SCF ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	auxin binding Ref.5 Inferred from sequence or structural similarity. Source: UniProtKB inositol hexakisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA12	Q38830	1	EBI-617501,EBI-617608
IAA7	Q38825	1	EBI-617501,EBI-602959

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Protein AUXIN SIGNALING F-BOX 3		PRO_0000272244
Regions
	Domain	1 – 45	45	F-box
	Region	76 – 77	2	Interaction with auxin-responsive proteins By similarity
	Region	108 – 109	2	Myo-inositol hexakisphosphate binding By similarity
	Region	343 – 348	6	Interaction with auxin-responsive proteins By similarity
	Region	398 – 400	3	Myo-inositol hexakisphosphate binding By similarity
	Region	402 – 406	5	Interaction with auxin-responsive proteins By similarity
	Region	461 – 462	2	Interaction with auxin-responsive proteins By similarity
	Region	481 – 482	2	Myo-inositol hexakisphosphate binding By similarity
Sites
	Binding site	69	1	Myo-inositol hexakisphosphate By similarity
	Binding site	340	1	Myo-inositol hexakisphosphate By similarity
	Binding site	433	1	Myo-inositol hexakisphosphate By similarity
	Binding site	506	1	Myo-inositol hexakisphosphate By similarity
	Site	134	1	Interaction with auxin-responsive proteins By similarity
	Site	160	1	Interaction with auxin-responsive proteins By similarity
	Site	377	1	Interaction with auxin-responsive proteins By similarity
	Site	486	1	Interaction with auxin-responsive proteins By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LPW7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8E419B4D83068661	FASTA	577	64,906
	10 20 30 40 50 60 MNYFPDEVIE HVFDFVASHK DRNSISLVCK SWHKIERFSR KEVFIGNCYA INPERLIRRF 70 80 90 100 110 120 PCLKSLTLKG KPHFADFNLV PHEWGGFVHP WIEALARSRV GLEELRLKRM VVTDESLDLL 130 140 150 160 170 180 SRSFANFKSL VLVSCEGFTT DGLASIAANC RHLRELDLQE NEIDDHRGQW LNCFPDSCTT 190 200 210 220 230 240 LMSLNFACLK GETNVAALER LVARSPNLKS LKLNRAVPLD ALARLMSCAP QLVDLGVGSY 250 260 270 280 290 300 ENEPDPESFA KLMTAIKKYT SLRSLSGFLE VAPLCLPAFY PICQNLISLN LSYAAEIQGN 310 320 330 340 350 360 HLIKLIQLCK RLQRLWILDS IGDKGLAVVA ATCKELQELR VFPSDVHGEE DNNASVTEVG 370 380 390 400 410 420 LVAISAGCPK LHSILYFCKQ MTNAALIAVA KNCPNFIRFR LCILEPHKPD HITFQSLDEG 430 440 450 460 470 480 FGAIVQACKG LRRLSVSGLL TDQVFLYIGM YAEQLEMLSI AFAGDTDKGM LYVLNGCKKM 490 500 510 520 530 540 RKLEIRDSPF GNAALLADVG RYETMRSLWM SSCEVTLGGC KRLAQNSPRL NVEIINENEN 550 560 570 NGMEQNEEDE REKVDKLYLY RTVVGTRKDA PPYVRIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Plant development is regulated by a family of auxin receptor F box proteins." Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M. Dev. Cell 9:109-119(2005) [PubMed: 15992545] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
[5]	"The F-box protein TIR1 is an auxin receptor." Dharmasiri N., Dharmasiri S., Estelle M. Nature 435:441-445(2005) [PubMed: 15917797] [Abstract] Cited for: FUNCTION.
[6]	"A plant miRNA contributes to antibacterial resistance by repressing auxin signaling." Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G. Science 312:436-439(2006) [PubMed: 16627744] [Abstract] Cited for: INDUCTION.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005172 Genomic DNA. Translation: AAF78487.1. AY099541 mRNA. Translation: AAM20393.1. BT002118 mRNA. Translation: AAN72129.1. AK227280 mRNA. Translation: BAE99304.1.
IPI	IPI00520794.
PIR	F86261.
RefSeq	NP_563915.1.
UniGene	At.71229
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LPW7. 4 interactions.
Proteomic databases
PRIDE	Q9LPW7.
Genome annotation databases
GeneID	837838.
GenomeReviews	Gene locus AT1G12820 in contig CT485782_GR.
KEGG	ath:AT1G12820.
NMPDR	fig|3702.1.peg.1550.
Organism-specific databases
TAIR	At1g12820.
Phylogenomic databases
OMA	Q9LPW7. RSFANFK.
Gene expression databases
ArrayExpress	Q9LPW7.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFB3_ARATH

Accession

Primary (citable) accession number: Q9LPW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)