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Protein

Protein AUXIN SIGNALING F-BOX 3

Gene

AFB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Confers sensitivity to the virulent bacterial pathogen P.syringae (By similarity). Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.By similarity2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691Myo-inositol hexakisphosphateBy similarity
Sitei134 – 1341Interaction with auxin-responsive proteinsBy similarity
Sitei160 – 1601Interaction with auxin-responsive proteinsBy similarity
Binding sitei340 – 3401Myo-inositol hexakisphosphateBy similarity
Sitei377 – 3771Interaction with auxin-responsive proteinsBy similarity
Binding sitei433 – 4331Myo-inositol hexakisphosphateBy similarity
Sitei486 – 4861Interaction with auxin-responsive proteinsBy similarity
Binding sitei506 – 5061Myo-inositol hexakisphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB
  • cellular response to nitrate Source: TAIR
  • defense response Source: UniProtKB-KW
  • lateral root development Source: TAIR
  • pollen maturation Source: TAIR
  • primary root development Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to molecule of bacterial origin Source: TAIR
  • stamen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Auxin signaling pathway, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AUXIN SIGNALING F-BOX 3
Gene namesi
Name:AFB3
Ordered Locus Names:At1g12820
ORF Names:F13K23.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G12820.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Protein AUXIN SIGNALING F-BOX 3PRO_0000272244Add
BLAST

Proteomic databases

PaxDbiQ9LPW7.
PRIDEiQ9LPW7.

Expressioni

Tissue specificityi

Ubiquitous, with higher levels in flowers.1 Publication

Inductioni

Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

Gene expression databases

GenevestigatoriQ9LPW7.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1 and SKP1A/ASK1. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner.1 Publication

Protein-protein interaction databases

BioGridi23078. 2 interactions.
IntActiQ9LPW7. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9LPW7.
SMRiQ9LPW7. Positions 5-574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4545F-boxAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 772Interaction with auxin-responsive proteinsBy similarity
Regioni108 – 1092Myo-inositol hexakisphosphate bindingBy similarity
Regioni343 – 3486Interaction with auxin-responsive proteinsBy similarity
Regioni398 – 4003Myo-inositol hexakisphosphate bindingBy similarity
Regioni402 – 4065Interaction with auxin-responsive proteinsBy similarity
Regioni461 – 4622Interaction with auxin-responsive proteinsBy similarity
Regioni481 – 4822Myo-inositol hexakisphosphate bindingBy similarity

Domaini

The F-box is necessary for the interaction with SKP1.By similarity

Sequence similaritiesi

Contains 1 F-box domain.Curated

Phylogenomic databases

eggNOGiNOG314738.
HOGENOMiHOG000239805.
InParanoidiQ9LPW7.
OMAiMEQNEED.
PhylomeDBiQ9LPW7.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LPW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYFPDEVIE HVFDFVASHK DRNSISLVCK SWHKIERFSR KEVFIGNCYA
60 70 80 90 100
INPERLIRRF PCLKSLTLKG KPHFADFNLV PHEWGGFVHP WIEALARSRV
110 120 130 140 150
GLEELRLKRM VVTDESLDLL SRSFANFKSL VLVSCEGFTT DGLASIAANC
160 170 180 190 200
RHLRELDLQE NEIDDHRGQW LNCFPDSCTT LMSLNFACLK GETNVAALER
210 220 230 240 250
LVARSPNLKS LKLNRAVPLD ALARLMSCAP QLVDLGVGSY ENEPDPESFA
260 270 280 290 300
KLMTAIKKYT SLRSLSGFLE VAPLCLPAFY PICQNLISLN LSYAAEIQGN
310 320 330 340 350
HLIKLIQLCK RLQRLWILDS IGDKGLAVVA ATCKELQELR VFPSDVHGEE
360 370 380 390 400
DNNASVTEVG LVAISAGCPK LHSILYFCKQ MTNAALIAVA KNCPNFIRFR
410 420 430 440 450
LCILEPHKPD HITFQSLDEG FGAIVQACKG LRRLSVSGLL TDQVFLYIGM
460 470 480 490 500
YAEQLEMLSI AFAGDTDKGM LYVLNGCKKM RKLEIRDSPF GNAALLADVG
510 520 530 540 550
RYETMRSLWM SSCEVTLGGC KRLAQNSPRL NVEIINENEN NGMEQNEEDE
560 570
REKVDKLYLY RTVVGTRKDA PPYVRIL
Length:577
Mass (Da):64,906
Last modified:October 1, 2000 - v1
Checksum:i8E419B4D83068661
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012187 Genomic DNA. Translation: AAF78487.1.
CP002684 Genomic DNA. Translation: AEE28934.1.
AY099541 mRNA. Translation: AAM20393.1.
BT002118 mRNA. Translation: AAN72129.1.
AK227280 mRNA. Translation: BAE99304.1.
PIRiF86261.
RefSeqiNP_563915.1. NM_101152.4.
UniGeneiAt.23978.
At.71229.

Genome annotation databases

EnsemblPlantsiAT1G12820.1; AT1G12820.1; AT1G12820.
GeneIDi837838.
KEGGiath:AT1G12820.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012187 Genomic DNA. Translation: AAF78487.1.
CP002684 Genomic DNA. Translation: AEE28934.1.
AY099541 mRNA. Translation: AAM20393.1.
BT002118 mRNA. Translation: AAN72129.1.
AK227280 mRNA. Translation: BAE99304.1.
PIRiF86261.
RefSeqiNP_563915.1. NM_101152.4.
UniGeneiAt.23978.
At.71229.

3D structure databases

ProteinModelPortaliQ9LPW7.
SMRiQ9LPW7. Positions 5-574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23078. 2 interactions.
IntActiQ9LPW7. 4 interactions.

Proteomic databases

PaxDbiQ9LPW7.
PRIDEiQ9LPW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G12820.1; AT1G12820.1; AT1G12820.
GeneIDi837838.
KEGGiath:AT1G12820.

Organism-specific databases

TAIRiAT1G12820.

Phylogenomic databases

eggNOGiNOG314738.
HOGENOMiHOG000239805.
InParanoidiQ9LPW7.
OMAiMEQNEED.
PhylomeDBiQ9LPW7.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9LPW7.

Gene expression databases

GenevestigatoriQ9LPW7.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Plant development is regulated by a family of auxin receptor F box proteins."
    Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
    Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
  6. "The F-box protein TIR1 is an auxin receptor."
    Dharmasiri N., Dharmasiri S., Estelle M.
    Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
    Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
    Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiAFB3_ARATH
AccessioniPrimary (citable) accession number: Q9LPW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.