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Q9LPW7

- AFB3_ARATH

UniProt

Q9LPW7 - AFB3_ARATH

Protein

Protein AUXIN SIGNALING F-BOX 3

Gene

AFB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity. Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.By similarity2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691Myo-inositol hexakisphosphateBy similarity
    Sitei134 – 1341Interaction with auxin-responsive proteinsBy similarity
    Sitei160 – 1601Interaction with auxin-responsive proteinsBy similarity
    Binding sitei340 – 3401Myo-inositol hexakisphosphateBy similarity
    Sitei377 – 3771Interaction with auxin-responsive proteinsBy similarity
    Binding sitei433 – 4331Myo-inositol hexakisphosphateBy similarity
    Sitei486 – 4861Interaction with auxin-responsive proteinsBy similarity
    Binding sitei506 – 5061Myo-inositol hexakisphosphateBy similarity

    GO - Molecular functioni

    1. auxin binding Source: UniProtKB
    2. inositol hexakisphosphate binding Source: UniProtKB

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB
    2. cellular response to nitrate Source: TAIR
    3. defense response Source: UniProtKB-KW
    4. lateral root development Source: TAIR
    5. pollen maturation Source: TAIR
    6. primary root development Source: TAIR
    7. protein ubiquitination Source: UniProtKB-UniPathway
    8. response to molecule of bacterial origin Source: TAIR
    9. stamen development Source: TAIR

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Auxin signaling pathway, Plant defense, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein AUXIN SIGNALING F-BOX 3
    Gene namesi
    Name:AFB3
    Ordered Locus Names:At1g12820
    ORF Names:F13K23.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G12820.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. SCF ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 577577Protein AUXIN SIGNALING F-BOX 3PRO_0000272244Add
    BLAST

    Proteomic databases

    PaxDbiQ9LPW7.
    PRIDEiQ9LPW7.

    Expressioni

    Tissue specificityi

    Ubiquitous, with higher levels in flowers.1 Publication

    Inductioni

    Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

    Gene expression databases

    ArrayExpressiQ9LPW7.
    GenevestigatoriQ9LPW7.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1 and SKP1A/ASK1. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner.1 Publication

    Protein-protein interaction databases

    BioGridi23078. 2 interactions.
    IntActiQ9LPW7. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LPW7.
    SMRiQ9LPW7. Positions 5-574.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 4545F-boxAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 772Interaction with auxin-responsive proteinsBy similarity
    Regioni108 – 1092Myo-inositol hexakisphosphate bindingBy similarity
    Regioni343 – 3486Interaction with auxin-responsive proteinsBy similarity
    Regioni398 – 4003Myo-inositol hexakisphosphate bindingBy similarity
    Regioni402 – 4065Interaction with auxin-responsive proteinsBy similarity
    Regioni461 – 4622Interaction with auxin-responsive proteinsBy similarity
    Regioni481 – 4822Myo-inositol hexakisphosphate bindingBy similarity

    Domaini

    The F-box is necessary for the interaction with SKP1.By similarity

    Sequence similaritiesi

    Contains 1 F-box domain.Curated

    Phylogenomic databases

    eggNOGiNOG314738.
    HOGENOMiHOG000239805.
    InParanoidiQ9LPW7.
    OMAiMEQNEED.
    PhylomeDBiQ9LPW7.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LPW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYFPDEVIE HVFDFVASHK DRNSISLVCK SWHKIERFSR KEVFIGNCYA    50
    INPERLIRRF PCLKSLTLKG KPHFADFNLV PHEWGGFVHP WIEALARSRV 100
    GLEELRLKRM VVTDESLDLL SRSFANFKSL VLVSCEGFTT DGLASIAANC 150
    RHLRELDLQE NEIDDHRGQW LNCFPDSCTT LMSLNFACLK GETNVAALER 200
    LVARSPNLKS LKLNRAVPLD ALARLMSCAP QLVDLGVGSY ENEPDPESFA 250
    KLMTAIKKYT SLRSLSGFLE VAPLCLPAFY PICQNLISLN LSYAAEIQGN 300
    HLIKLIQLCK RLQRLWILDS IGDKGLAVVA ATCKELQELR VFPSDVHGEE 350
    DNNASVTEVG LVAISAGCPK LHSILYFCKQ MTNAALIAVA KNCPNFIRFR 400
    LCILEPHKPD HITFQSLDEG FGAIVQACKG LRRLSVSGLL TDQVFLYIGM 450
    YAEQLEMLSI AFAGDTDKGM LYVLNGCKKM RKLEIRDSPF GNAALLADVG 500
    RYETMRSLWM SSCEVTLGGC KRLAQNSPRL NVEIINENEN NGMEQNEEDE 550
    REKVDKLYLY RTVVGTRKDA PPYVRIL 577
    Length:577
    Mass (Da):64,906
    Last modified:October 1, 2000 - v1
    Checksum:i8E419B4D83068661
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012187 Genomic DNA. Translation: AAF78487.1.
    CP002684 Genomic DNA. Translation: AEE28934.1.
    AY099541 mRNA. Translation: AAM20393.1.
    BT002118 mRNA. Translation: AAN72129.1.
    AK227280 mRNA. Translation: BAE99304.1.
    PIRiF86261.
    RefSeqiNP_563915.1. NM_101152.4.
    UniGeneiAt.23978.
    At.71229.

    Genome annotation databases

    EnsemblPlantsiAT1G12820.1; AT1G12820.1; AT1G12820.
    GeneIDi837838.
    KEGGiath:AT1G12820.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012187 Genomic DNA. Translation: AAF78487.1 .
    CP002684 Genomic DNA. Translation: AEE28934.1 .
    AY099541 mRNA. Translation: AAM20393.1 .
    BT002118 mRNA. Translation: AAN72129.1 .
    AK227280 mRNA. Translation: BAE99304.1 .
    PIRi F86261.
    RefSeqi NP_563915.1. NM_101152.4.
    UniGenei At.23978.
    At.71229.

    3D structure databases

    ProteinModelPortali Q9LPW7.
    SMRi Q9LPW7. Positions 5-574.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23078. 2 interactions.
    IntActi Q9LPW7. 4 interactions.

    Proteomic databases

    PaxDbi Q9LPW7.
    PRIDEi Q9LPW7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G12820.1 ; AT1G12820.1 ; AT1G12820 .
    GeneIDi 837838.
    KEGGi ath:AT1G12820.

    Organism-specific databases

    TAIRi AT1G12820.

    Phylogenomic databases

    eggNOGi NOG314738.
    HOGENOMi HOG000239805.
    InParanoidi Q9LPW7.
    OMAi MEQNEED.
    PhylomeDBi Q9LPW7.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Gene expression databases

    ArrayExpressi Q9LPW7.
    Genevestigatori Q9LPW7.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Plant development is regulated by a family of auxin receptor F box proteins."
      Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
      Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
    6. "The F-box protein TIR1 is an auxin receptor."
      Dharmasiri N., Dharmasiri S., Estelle M.
      Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
      Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
      Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiAFB3_ARATH
    AccessioniPrimary (citable) accession number: Q9LPW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3