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Q9LPW7 (AFB3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein AUXIN SIGNALING F-BOX 3
Gene names
Name:AFB3
Ordered Locus Names:At1g12820
ORF Names:F13K23.7
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity.Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin. Ref.5 Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1 and SKP1A/ASK1. Interacts with Aux/IAA proteins (IAA7 and IAA12) in an auxin-dependent manner. Ref.5

Subcellular location

Nucleus Ref.5.

Tissue specificity

Ubiquitous, with higher levels in flowers. Ref.5

Induction

Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22). Ref.7

Domain

The F-box is necessary for the interaction with SKP1 By similarity.

Sequence similarities

Contains 1 F-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Protein AUXIN SIGNALING F-BOX 3
PRO_0000272244

Regions

Domain1 – 4545F-box
Region76 – 772Interaction with auxin-responsive proteins By similarity
Region108 – 1092Myo-inositol hexakisphosphate binding By similarity
Region343 – 3486Interaction with auxin-responsive proteins By similarity
Region398 – 4003Myo-inositol hexakisphosphate binding By similarity
Region402 – 4065Interaction with auxin-responsive proteins By similarity
Region461 – 4622Interaction with auxin-responsive proteins By similarity
Region481 – 4822Myo-inositol hexakisphosphate binding By similarity

Sites

Binding site691Myo-inositol hexakisphosphate By similarity
Binding site3401Myo-inositol hexakisphosphate By similarity
Binding site4331Myo-inositol hexakisphosphate By similarity
Binding site5061Myo-inositol hexakisphosphate By similarity
Site1341Interaction with auxin-responsive proteins By similarity
Site1601Interaction with auxin-responsive proteins By similarity
Site3771Interaction with auxin-responsive proteins By similarity
Site4861Interaction with auxin-responsive proteins By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LPW7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 8E419B4D83068661

FASTA57764,906
        10         20         30         40         50         60 
MNYFPDEVIE HVFDFVASHK DRNSISLVCK SWHKIERFSR KEVFIGNCYA INPERLIRRF 

        70         80         90        100        110        120 
PCLKSLTLKG KPHFADFNLV PHEWGGFVHP WIEALARSRV GLEELRLKRM VVTDESLDLL 

       130        140        150        160        170        180 
SRSFANFKSL VLVSCEGFTT DGLASIAANC RHLRELDLQE NEIDDHRGQW LNCFPDSCTT 

       190        200        210        220        230        240 
LMSLNFACLK GETNVAALER LVARSPNLKS LKLNRAVPLD ALARLMSCAP QLVDLGVGSY 

       250        260        270        280        290        300 
ENEPDPESFA KLMTAIKKYT SLRSLSGFLE VAPLCLPAFY PICQNLISLN LSYAAEIQGN 

       310        320        330        340        350        360 
HLIKLIQLCK RLQRLWILDS IGDKGLAVVA ATCKELQELR VFPSDVHGEE DNNASVTEVG 

       370        380        390        400        410        420 
LVAISAGCPK LHSILYFCKQ MTNAALIAVA KNCPNFIRFR LCILEPHKPD HITFQSLDEG 

       430        440        450        460        470        480 
FGAIVQACKG LRRLSVSGLL TDQVFLYIGM YAEQLEMLSI AFAGDTDKGM LYVLNGCKKM 

       490        500        510        520        530        540 
RKLEIRDSPF GNAALLADVG RYETMRSLWM SSCEVTLGGC KRLAQNSPRL NVEIINENEN 

       550        560        570 
NGMEQNEEDE REKVDKLYLY RTVVGTRKDA PPYVRIL

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Plant development is regulated by a family of auxin receptor F box proteins."
Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
Dev. Cell 9:109-119(2005) [PubMed: 15992545] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH CUL1; IAA7; IAA12 AND SKP1A/ASK1.
[6]"The F-box protein TIR1 is an auxin receptor."
Dharmasiri N., Dharmasiri S., Estelle M.
Nature 435:441-445(2005) [PubMed: 15917797] [Abstract]
Cited for: FUNCTION.
[7]"A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
Science 312:436-439(2006) [PubMed: 16627744] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC012187 Genomic DNA. Translation: AAF78487.1.
CP002684 Genomic DNA. Translation: AEE28934.1.
AY099541 mRNA. Translation: AAM20393.1.
BT002118 mRNA. Translation: AAN72129.1.
AK227280 mRNA. Translation: BAE99304.1.
IPIIPI00520794.
PIRF86261.
RefSeqNP_563915.1. NM_101152.4.
UniGeneAt.23978.
At.71229.

3D structure databases

ProteinModelPortalQ9LPW7.
SMRQ9LPW7. Positions 3-577.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9LPW7. 4 interactions.
STRINGQ9LPW7.

Proteomic databases

PRIDEQ9LPW7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G12820.1; AT1G12820.1; AT1G12820.
GeneID837838.
GenomeReviewsGene locus AT1G12820 in contig CT485782_GR.
KEGGath:AT1G12820.
NMPDRfig|3702.1.peg.1550.

Organism-specific databases

TAIRAt1g12820.

Phylogenomic databases

GeneTreeEPGT00050000007569.
HOGENOMHBG319412.
InParanoidQ9LPW7.
OMAIINENEN.
PhylomeDBQ9LPW7.
ProtClustDBCLSN2687785.

Gene expression databases

ArrayExpressQ9LPW7.
GenevestigatorQ9LPW7.

Family and domain databases

InterProIPR001810. F-box_dom_cyclin-like.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
PROSITEPS50181. FBOX. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAFB3_ARATH
AccessionPrimary (citable) accession number: Q9LPW7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: October 19, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents