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Protein

Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplastic

Gene

GAPA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Catalyzes the reduction of 1,3-diphosphoglycerate by NADPH (By similarity).By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NADPBy similarity
Binding sitei143 – 1431NADP; via carbonyl oxygenBy similarity
Active sitei216 – 2161NucleophilePROSITE-ProRule annotation
Sitei243 – 2431Activates thiol group during catalysisBy similarity
Binding sitei246 – 2461Glyceraldehyde 3-phosphateBy similarity
Binding sitei297 – 2971Glyceraldehyde 3-phosphateBy similarity
Binding sitei379 – 3791NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 752NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:GQT-230-MONOMER.
ARA:GQT-231-MONOMER.
ARA:GQT-232-MONOMER.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase A subunit 2
Gene namesi
Name:GAPA2
Ordered Locus Names:At1g12900
ORF Names:F13K23.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G12900.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848ChloroplastSequence analysisAdd
BLAST
Chaini49 – 399351Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplasticPRO_0000422409Add
BLAST

Proteomic databases

PaxDbiQ9LPW0.
PRIDEiQ9LPW0.
ProMEXiQ9LPW0.

PTM databases

iPTMnetiQ9LPW0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LPW0. baseline and differential.
GenevisibleiQ9LPW0. AT.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

BioGridi23088. 6 interactions.
IntActiQ9LPW0. 2 interactions.
STRINGi3702.AT1G12900.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LPW0.
SMRiQ9LPW0. Positions 63-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni215 – 2173Glyceraldehyde 3-phosphate bindingBy similarity
Regioni274 – 2752Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiQ9LPW0.
KOiK05298.
OMAiVDFVFES.
OrthoDBiEOG09360CQC.
PhylomeDBiQ9LPW0.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LPW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASATFSVAK PSLQGFSEFS GLRNSSALPF AKRSSSDEFV SFVSFQTSAM
60 70 80 90 100
RSNGGYRKGV TEAKIKVAIN GFGRIGRNFL RCWHGRKDSP LDVVVINDTG
110 120 130 140 150
GVKQASHLLK YDSTLGIFDA DVKPSGDSAL SVDGKIIKIV SDRNPSNLPW
160 170 180 190 200
GELGIDLVIE GTGVFVDRDG AGKHLQAGAK KVLITAPGKG DIPTYVVGVN
210 220 230 240 250
AELYSHEDTI ISNASCTTNC LAPFVKVLDQ KFGIIKGTMT TTHSYTGDQR
260 270 280 290 300
LLDASHRDLR RARAAALNIV PTSTGAAKAV ALVLPNLKGK LNGIALRVPT
310 320 330 340 350
PNVSVVDLVV QVSKKTFAEE VNAAFRDAAE KELKGILDVC DEPLVSVDFR
360 370 380 390
CSDVSSTIDS SLTMVMGDDM VKVIAWYDNE WGYSQRVVDL ADIVANNWK
Length:399
Mass (Da):42,847
Last modified:October 1, 2000 - v1
Checksum:iEC5EDDFA8A58295D
GO
Isoform 2 (identifier: Q9LPW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:350
Mass (Da):37,667
Checksum:i6F8D17929BFD2C60
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191D → G in BAH57068 (PubMed:19423640).Curated
Sequence conflicti387 – 3871V → D in BAH57068 (PubMed:19423640).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949Missing in isoform 2. 1 PublicationVSP_046526Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012187 Genomic DNA. Translation: AAF78494.1.
CP002684 Genomic DNA. Translation: AEE28944.1.
CP002684 Genomic DNA. Translation: AEE28946.1.
CP002684 Genomic DNA. Translation: AEE28947.1.
BT008637 mRNA. Translation: AAP40454.1.
AK318953 mRNA. Translation: BAH57068.1.
BT015867 mRNA. Translation: AAU94430.1.
PIRiF86262.
RefSeqiNP_001117276.1. NM_001123804.1. [Q9LPW0-2]
NP_001184977.1. NM_001198048.1. [Q9LPW0-2]
NP_172750.1. NM_101161.3. [Q9LPW0-1]
UniGeneiAt.28212.

Genome annotation databases

EnsemblPlantsiAT1G12900.1; AT1G12900.1; AT1G12900. [Q9LPW0-1]
GeneIDi837848.
KEGGiath:AT1G12900.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012187 Genomic DNA. Translation: AAF78494.1.
CP002684 Genomic DNA. Translation: AEE28944.1.
CP002684 Genomic DNA. Translation: AEE28946.1.
CP002684 Genomic DNA. Translation: AEE28947.1.
BT008637 mRNA. Translation: AAP40454.1.
AK318953 mRNA. Translation: BAH57068.1.
BT015867 mRNA. Translation: AAU94430.1.
PIRiF86262.
RefSeqiNP_001117276.1. NM_001123804.1. [Q9LPW0-2]
NP_001184977.1. NM_001198048.1. [Q9LPW0-2]
NP_172750.1. NM_101161.3. [Q9LPW0-1]
UniGeneiAt.28212.

3D structure databases

ProteinModelPortaliQ9LPW0.
SMRiQ9LPW0. Positions 63-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23088. 6 interactions.
IntActiQ9LPW0. 2 interactions.
STRINGi3702.AT1G12900.1.

PTM databases

iPTMnetiQ9LPW0.

Proteomic databases

PaxDbiQ9LPW0.
PRIDEiQ9LPW0.
ProMEXiQ9LPW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G12900.1; AT1G12900.1; AT1G12900. [Q9LPW0-1]
GeneIDi837848.
KEGGiath:AT1G12900.

Organism-specific databases

TAIRiAT1G12900.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiQ9LPW0.
KOiK05298.
OMAiVDFVFES.
OrthoDBiEOG09360CQC.
PhylomeDBiQ9LPW0.

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciARA:GQT-230-MONOMER.
ARA:GQT-231-MONOMER.
ARA:GQT-232-MONOMER.

Miscellaneous databases

PROiQ9LPW0.

Gene expression databases

ExpressionAtlasiQ9LPW0. baseline and differential.
GenevisibleiQ9LPW0. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA2_ARATH
AccessioniPrimary (citable) accession number: Q9LPW0
Secondary accession number(s): B3H4P2, C0Z2Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-depedent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.