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Protein

Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplastic

Gene

GAPA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Catalyzes the reduction of 1,3-diphosphoglycerate by NADPH (By similarity).By similarity

Miscellaneous

Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-dependent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98NADPBy similarity1
Binding sitei143NADP; via carbonyl oxygenBy similarity1
Active sitei216NucleophilePROSITE-ProRule annotation1
Sitei243Activates thiol group during catalysisBy similarity1
Binding sitei246Glyceraldehyde 3-phosphateBy similarity1
Binding sitei297Glyceraldehyde 3-phosphateBy similarity1
Binding sitei379NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 75NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCalvin cycle
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00116

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase A subunit 2
Gene namesi
Name:GAPA2
Ordered Locus Names:At1g12900
ORF Names:F13K23.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G12900
TAIRilocus:2010361 AT1G12900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000042240949 – 399Glyceraldehyde-3-phosphate dehydrogenase GAPA2, chloroplasticAdd BLAST351

Proteomic databases

PaxDbiQ9LPW0
PRIDEiQ9LPW0
ProMEXiQ9LPW0

PTM databases

iPTMnetiQ9LPW0

Expressioni

Gene expression databases

ExpressionAtlasiQ9LPW0 baseline and differential
GenevisibleiQ9LPW0 AT

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

BioGridi23088, 6 interactors
IntActiQ9LPW0, 2 interactors
MINTiQ9LPW0
STRINGi3702.AT1G12900.1

Structurei

3D structure databases

ProteinModelPortaliQ9LPW0
SMRiQ9LPW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 217Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni274 – 275Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657 Eukaryota
COG0057 LUCA
HOGENOMiHOG000071679
InParanoidiQ9LPW0
KOiK05298
OMAiNAKVLAW
OrthoDBiEOG09360CQC
PhylomeDBiQ9LPW0

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LPW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASATFSVAK PSLQGFSEFS GLRNSSALPF AKRSSSDEFV SFVSFQTSAM
60 70 80 90 100
RSNGGYRKGV TEAKIKVAIN GFGRIGRNFL RCWHGRKDSP LDVVVINDTG
110 120 130 140 150
GVKQASHLLK YDSTLGIFDA DVKPSGDSAL SVDGKIIKIV SDRNPSNLPW
160 170 180 190 200
GELGIDLVIE GTGVFVDRDG AGKHLQAGAK KVLITAPGKG DIPTYVVGVN
210 220 230 240 250
AELYSHEDTI ISNASCTTNC LAPFVKVLDQ KFGIIKGTMT TTHSYTGDQR
260 270 280 290 300
LLDASHRDLR RARAAALNIV PTSTGAAKAV ALVLPNLKGK LNGIALRVPT
310 320 330 340 350
PNVSVVDLVV QVSKKTFAEE VNAAFRDAAE KELKGILDVC DEPLVSVDFR
360 370 380 390
CSDVSSTIDS SLTMVMGDDM VKVIAWYDNE WGYSQRVVDL ADIVANNWK
Length:399
Mass (Da):42,847
Last modified:October 1, 2000 - v1
Checksum:iEC5EDDFA8A58295D
GO
Isoform 2 (identifier: Q9LPW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:350
Mass (Da):37,667
Checksum:i6F8D17929BFD2C60
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119D → G in BAH57068 (PubMed:19423640).Curated1
Sequence conflicti387V → D in BAH57068 (PubMed:19423640).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0465261 – 49Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012187 Genomic DNA Translation: AAF78494.1
CP002684 Genomic DNA Translation: AEE28944.1
CP002684 Genomic DNA Translation: AEE28946.1
CP002684 Genomic DNA Translation: AEE28947.1
BT008637 mRNA Translation: AAP40454.1
AK318953 mRNA Translation: BAH57068.1
BT015867 mRNA Translation: AAU94430.1
PIRiF86262
RefSeqiNP_001117276.1, NM_001123804.1 [Q9LPW0-2]
NP_001184977.1, NM_001198048.1 [Q9LPW0-2]
NP_172750.1, NM_101161.3 [Q9LPW0-1]
UniGeneiAt.28212

Genome annotation databases

EnsemblPlantsiAT1G12900.1; AT1G12900.1; AT1G12900 [Q9LPW0-1]
AT1G12900.3; AT1G12900.3; AT1G12900 [Q9LPW0-2]
AT1G12900.4; AT1G12900.4; AT1G12900 [Q9LPW0-2]
AT1G12900.5; AT1G12900.5; AT1G12900
GeneIDi837848
GrameneiAT1G12900.1; AT1G12900.1; AT1G12900 [Q9LPW0-1]
AT1G12900.3; AT1G12900.3; AT1G12900 [Q9LPW0-2]
AT1G12900.4; AT1G12900.4; AT1G12900 [Q9LPW0-2]
AT1G12900.5; AT1G12900.5; AT1G12900
KEGGiath:AT1G12900

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiG3PA2_ARATH
AccessioniPrimary (citable) accession number: Q9LPW0
Secondary accession number(s): B3H4P2, C0Z2Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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