Q9LPS1 (HXK3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-3
EC=2.7.1.1

Gene names

Ordered Locus Names:	At1g50460
ORF Names:	F11F12.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol By similarity.
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Ref.4 Ref.5.
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to cold Inferred from expression pattern. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Hexokinase-3		PRO_0000259631
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Nucleotide binding	101 – 106	6	ATP Potential
	Region	170 – 196	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LPS1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1818AD18A17063D6	FASTA	498	54,591
	10 20 30 40 50 60 MGKVAVAFAA VAVVAACSVA AVMVGRRMKS RRKWRTVVEI LKELEDDCDT PVGRLRQVVD 70 80 90 100 110 120 AMAVEMHAGL ASEGGSKLKM LLTFVDDLPT GREKGTYYAL HLGGTYFRIL RVLLGDQRSY 130 140 150 160 170 180 LDVQDVERHP IPSHLMNSTS EVLFNFLAFS LERFIEKEEN GSDSQGVRRE LAFTFSFPVK 190 200 210 220 230 240 HTSISSGVLI KWTKGFEISE MVGQDIAECL QGALNRRGLD MHVAALVNDT VGALSLGYYH 250 260 270 280 290 300 DPDTVVAVVF GTGSNACYLE RTDAIIKCQG LLTTSGSMVV NMEWGNFWSS HLPRTSYDID 310 320 330 340 350 360 LDAESSNAND MGFEKMISGM YLGDIVRRVI LRMSEDSDIF GPISPVLSEP YVLRTNSVSA 370 380 390 400 410 420 IHEDDTPELQ EVARILKDIG VSDVPLKVRK LVVKICDVVT RRAGRLAAAG IAGILKKIGR 430 440 450 460 470 480 DGSGGITSGR SRSEIQMQKR TVVAVEGGLY MNYTMFREYM EEALVEILGE EVSQYVVVKA 490 MEDGSSIGSA LLVASLQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells." Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J. Plant Cell 15:2140-2151(2003) [PubMed: 12953116] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC012561 Genomic DNA. Translation: AAF87885.1. CP002684 Genomic DNA. Translation: AEE32553.1. AY074314 mRNA. Translation: AAL67011.1. AY096416 mRNA. Translation: AAM20056.1.
IPI	IPI00534749.
PIR	A96541.
RefSeq	NP_175463.1. NM_103929.3.
UniGene	At.27557.
3D structure databases
HSSP	HSSP built from PDB template 1HKB based on UniProtKB P19367.
ProteinModelPortal	Q9LPS1.
SMR	Q9LPS1. Positions 8-497.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LPS1.
Proteomic databases
PRIDE	Q9LPS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G50460.1; AT1G50460.1; AT1G50460.
GeneID	841468.
GenomeReviews	Gene locus AT1G50460 in contig CT485782_GR.
KEGG	ath:AT1G50460.
NMPDR	fig|3702.1.peg.4520.
Organism-specific databases
GeneFarm	4245. 430.
TAIR	At1g50460.
Phylogenomic databases
eggNOG	KOG1369.
GeneTree	EPGT00050000013968.
HOGENOM	HBG522186.
InParanoid	Q9LPS1.
OMA	YTMFREY.
PhylomeDB	Q9LPS1.
ProtClustDB	PLN02362.
Gene expression databases
ArrayExpress	Q9LPS1.
Genevestigator	Q9LPS1.
GermOnline	AT1G50460. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR022672. Hexokinase_N. [Graphical view]
KO	K00844.
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProtoNet	Search...

Entry information

Entry name

HXK3_ARATH

Accession

Primary (citable) accession number: Q9LPS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program