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Protein

Mitogen-activated protein kinase kinase 7

Gene

MKK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a negative regulator of polar auxin transport. Positively regulates plant basal and systemic acquired resistance (SAR). Activates MPK3 and MPK6 in vitro.3 Publications

Miscellaneous

Overexpression of MKK5 (mutant bud1) causes deficiency in polar auxin transport and leads to plant architectural abnormality (PubMed:16377756). The bud1 mutant accumulates elevated levels of SA, and exhibits constitutive PR gene expression and enhanced resistance to both bacterial and oomycete pathogens (PubMed:17908155).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74ATPCurated1
Active sitei165Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 59ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • MAP kinase kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • auxin polar transport Source: TAIR
  • defense response to bacterium Source: TAIR
  • defense response to oomycetes Source: TAIR
  • regulation of mitotic cell cycle Source: GO_Central
  • stress-activated protein kinase signaling cascade Source: GO_Central
  • systemic acquired resistance, salicylic acid mediated signaling pathway Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 7 (EC:2.7.12.2)
Short name:
AtMKK7
Short name:
MAP kinase kinase 7
Alternative name(s):
Protein BUSHY AND DWARF 1
Gene namesi
Name:MKK7
Synonyms:BUD1
Ordered Locus Names:At1g18350
ORF Names:F15H18.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G18350.
TAIRilocus:2014099. AT1G18350.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi74K → R: Abolishes kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004286231 – 307Mitogen-activated protein kinase kinase 7Add BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphoserineBy similarity1
Modified residuei203PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-193 and Ser-199 by MAP kinase kinase kinases positively regulates kinase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LPQ3.

Expressioni

Tissue specificityi

Expressed in all tissues, with a relatively higher level in leaves and lower level in roots and flowers.1 Publication

Inductioni

By Pseudomonas syringae pv. tomato (Pst) DC3000/avrRpt2 infection.1 Publication

Gene expression databases

ExpressionAtlasiQ9LPQ3. baseline and differential.
GenevisibleiQ9LPQ3. AT.

Interactioni

Subunit structurei

Interacts with MPK15.1 Publication

Protein-protein interaction databases

DIPiDIP-52434N.
IntActiQ9LPQ3. 3 interactors.
STRINGi3702.AT1G18350.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LPQ3.
SMRiQ9LPQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 303Protein kinasePROSITE-ProRule annotationAdd BLAST259

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9LPQ3.
OMAiLKVLHQG.
OrthoDBiEOG09360IH3.
PhylomeDBiQ9LPQ3.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LPQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVRKRRQI NLRLPVPPLS VHLPWFSFAS STAPVINNGI SASDVEKLHV
60 70 80 90 100
LGRGSSGIVY KVHHKTTGEI YALKSVNGDM SPAFTRQLAR EMEILRRTDS
110 120 130 140 150
PYVVRCQGIF EKPIVGEVSI LMEYMDGGNL ESLRGAVTEK QLAGFSRQIL
160 170 180 190 200
KGLSYLHSLK IVHRDIKPAN LLLNSRNEVK IADFGVSKII TRSLDYCNSY
210 220 230 240 250
VGTCAYMSPE RFDSAAGENS DVYAGDIWSF GVMILELFVG HFPLLPQGQR
260 270 280 290 300
PDWATLMCVV CFGEPPRAPE GCSDEFRSFV DCCLRKESSE RWTASQLLGH

PFLRESL
Length:307
Mass (Da):34,272
Last modified:October 1, 2000 - v1
Checksum:i7F831976745C4FEA
GO

Sequence cautioni

The sequence ABK28402 differs from that shown. Reason: Erroneous termination at position 308. Translated as stop.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ185389 mRNA. Translation: ABA70752.1.
AC013354 Genomic DNA. Translation: AAF25995.1.
CP002684 Genomic DNA. Translation: AEE29706.1.
DQ446261 mRNA. Translation: ABE65631.1.
DQ652843 mRNA. Translation: ABK28402.1. Sequence problems.
RefSeqiNP_173271.1. NM_101693.3.
UniGeneiAt.28338.
At.51662.

Genome annotation databases

EnsemblPlantsiAT1G18350.1; AT1G18350.1; AT1G18350.
GeneIDi838416.
GrameneiAT1G18350.1; AT1G18350.1; AT1G18350.
KEGGiath:AT1G18350.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiM2K7_ARATH
AccessioniPrimary (citable) accession number: Q9LPQ3
Secondary accession number(s): A0ME75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families