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Protein

Branched-chain-amino-acid aminotransferase 6

Gene

BCAT6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Acts on leucine, isoleucine and valine (By similarity).By similarity

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810), Acetolactate synthase (AXX17_At3g42690)
  2. Ketol-acid reductoisomerase (AXX17_At3g53150), Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase 2, chloroplastic (IPMS2), 2-isopropylmalate synthase 1, chloroplastic (IPMS1)
  2. 3-isopropylmalate dehydratase small subunit 1 (IPMI2), 3-isopropylmalate dehydratase small subunit 3 (At2g43090), 3-isopropylmalate dehydratase small subunit 2 (IPMI1), 3-isopropylmalate dehydratase large subunit, chloroplastic (IIL1)
  3. 3-isopropylmalate dehydrogenase (At1g31180/F28K20_14), 3-isopropylmalate dehydrogenase (IMD1), 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3), 3-isopropylmalate dehydrogenase 2, chloroplastic (IMDH2), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase (AXX17_At1g31810), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (AXX17_At5g13660), 3-isopropylmalate dehydrogenase (AXX17_At1g75410), 3-isopropylmalate dehydrogenase (IMD3), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase 1, chloroplastic (IMDH1)
  4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810), Acetolactate synthase (AXX17_At3g42690)
  2. Ketol-acid reductoisomerase (AXX17_At3g53150), Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • L-isoleucine transaminase activity Source: TAIR
  • L-leucine transaminase activity Source: TAIR
  • L-valine transaminase activity Source: TAIR
  • methionine-oxo-acid transaminase activity Source: TAIR

GO - Biological processi

  • glucosinolate biosynthetic process from homomethionine Source: TAIR
  • isoleucine biosynthetic process Source: UniProtKB-UniPathway
  • leucine biosynthetic process Source: UniProtKB-UniPathway
  • L-methionine salvage Source: TAIR
  • valine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT1G50110-MONOMER
ReactomeiR-ATH-70895 Branched-chain amino acid catabolism
UniPathwayiUPA00047; UER00058
UPA00048; UER00073
UPA00049; UER00062

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase 6 (EC:2.6.1.42)
Short name:
Atbcat-6
Gene namesi
Name:BCAT6
Ordered Locus Names:At1g50110
ORF Names:F2J10.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G50110
TAIRilocus:2031030 AT1G50110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001032981 – 356Branched-chain-amino-acid aminotransferase 6Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9LPM9
PRIDEiQ9LPM9

PTM databases

iPTMnetiQ9LPM9

Expressioni

Gene expression databases

ExpressionAtlasiQ9LPM9 baseline and differential
GenevisibleiQ9LPM9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G50110.1

Structurei

3D structure databases

ProteinModelPortaliQ9LPM9
SMRiQ9LPM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975 Eukaryota
COG0115 LUCA
HOGENOMiHOG000276704
InParanoidiQ9LPM9
KOiK00826
OMAiDYHKVSS
OrthoDBiEOG09360CSJ
PhylomeDBiQ9LPM9

Family and domain databases

CDDicd01557 BCAT_beta_family, 1 hit
InterProiView protein in InterPro
IPR001544 Aminotrans_IV
IPR018300 Aminotrans_IV_CS
IPR036038 Aminotransferase-like
IPR005786 B_amino_transII
IPR033939 BCAT_family
PfamiView protein in Pfam
PF01063 Aminotran_4, 1 hit
PIRSFiPIRSF006468 BCAT1, 1 hit
SUPFAMiSSF56752 SSF56752, 1 hit
TIGRFAMsiTIGR01123 ilvE_II, 1 hit
PROSITEiView protein in PROSITE
PS00770 AA_TRANSFER_CLASS_4, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LPM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSSSPLRT TSETDEKYAN VKWEELGFAL TPIDYMYVAK CRQGESFTQG
60 70 80 90 100
KIVPYGDISI SPCSPILNYG QGLFEGLKAY RTEDDRIRIF RPDQNALRMQ
110 120 130 140 150
TGAERLCMTP PTLEQFVEAV KQTVLANKKW VPPPGKGTLY IRPLLLGSGA
160 170 180 190 200
TLGVAPAPEY TFLIYASPVG DYHKVSSGLN LKVDHKYHRA HSGGTGGVKS
210 220 230 240 250
CTNYSPVVKS LLEAKSAGFS DVLFLDAATG RNIEELTACN IFIVKGNIVS
260 270 280 290 300
TPPTSGTILP GVTRKSISEL AHDIGYQVEE RDVSVDELLE AEEVFCTGTA
310 320 330 340 350
VVVKAVETVT FHDKKVKYRT GEAALSTKLH SMLTNIQMGV VEDKKGWMVD

IDPCQG
Length:356
Mass (Da):38,861
Last modified:October 1, 2000 - v1
Checksum:iC3F1A506BD3B82BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135 – 140GKGTLY → VKELCI in CAA81418 (PubMed:8580968).Curated6
Sequence conflicti145L → P in AAM66943 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ312747 mRNA Translation: CAC37393.1
AC015445 Genomic DNA Translation: AAF76437.1
CP002684 Genomic DNA Translation: AEE32516.1
AY088621 mRNA Translation: AAM66943.1
Z26805 mRNA Translation: CAA81418.1
PIRiD96537
RefSeqiNP_175431.1, NM_103897.4
UniGeneiAt.10368
At.67645

Genome annotation databases

EnsemblPlantsiAT1G50110.1; AT1G50110.1; AT1G50110
GeneIDi841433
GrameneiAT1G50110.1; AT1G50110.1; AT1G50110
KEGGiath:AT1G50110

Similar proteinsi

Entry informationi

Entry nameiBCAT6_ARATH
AccessioniPrimary (citable) accession number: Q9LPM9
Secondary accession number(s): Q42148, Q8L961
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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