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Protein

Branched-chain-amino-acid aminotransferase 6

Gene

BCAT6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Acts on leucine, isoleucine and valine (By similarity).By similarity

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810)
  2. Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-leucine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase 2, chloroplastic (IPMS2), 2-isopropylmalate synthase 1, chloroplastic (IPMS1)
  2. 3-isopropylmalate dehydratase small subunit 1 (IPMI2), 3-isopropylmalate dehydratase small subunit 3 (At2g43090), 3-isopropylmalate dehydratase small subunit 2 (IPMI1), 3-isopropylmalate dehydratase large subunit (IIL1)
  3. 3-isopropylmalate dehydrogenase (At1g31180/F28K20_14), 3-isopropylmalate dehydrogenase, 3-isopropylmalate dehydrogenase (IMD1), 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3), 3-isopropylmalate dehydrogenase 2, chloroplastic (IMDH2), 3-isopropylmalate dehydrogenase 1, chloroplastic (IMDH1), 3-isopropylmalate dehydrogenase (At5g14200), 3-isopropylmalate dehydrogenase
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit 1, chloroplastic (VAT1), Acetolactate synthase, chloroplastic (ALS), Acetolactate synthase small subunit 2, chloroplastic (At2g31810)
  2. Ketol-acid reductoisomerase, chloroplastic (At3g58610), Ketol-acid reductoisomerase (At3g58610)
  3. Dihydroxy-acid dehydratase, chloroplastic (DHAD)
  4. Branched-chain-amino-acid aminotransferase 6 (BCAT6), Branched-chain-amino-acid aminotransferase 2, chloroplastic (BCAT2), Branched-chain-amino-acid aminotransferase 5, chloroplastic (BCAT5), Branched-chain-amino-acid aminotransferase 3, chloroplastic (BCAT3)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • branched-chain-amino-acid transaminase activity Source: GO_Central
  • L-isoleucine transaminase activity Source: TAIR
  • L-leucine transaminase activity Source: TAIR
  • L-valine transaminase activity Source: TAIR
  • methionine-oxo-acid transaminase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • aspartate biosynthetic process Source: GO_Central
  • glucosinolate biosynthetic process from homomethionine Source: TAIR
  • isoleucine biosynthetic process Source: UniProtKB-UniPathway
  • leucine biosynthetic process Source: GO_Central
  • L-methionine salvage Source: TAIR
  • valine biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT1G50110-MONOMER.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00047; UER00058.
UPA00048; UER00073.
UPA00049; UER00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase 6 (EC:2.6.1.42)
Short name:
Atbcat-6
Gene namesi
Name:BCAT6
Ordered Locus Names:At1g50110
ORF Names:F2J10.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G50110.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Branched-chain-amino-acid aminotransferase 6PRO_0000103298Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ9LPM9.
PRIDEiQ9LPM9.

PTM databases

iPTMnetiQ9LPM9.

Expressioni

Gene expression databases

GenevisibleiQ9LPM9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G50110.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LPM9.
SMRiQ9LPM9. Positions 20-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
InParanoidiQ9LPM9.
KOiK00826.
OMAiKVKYRTG.
OrthoDBiEOG09360CSJ.
PhylomeDBiQ9LPM9.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LPM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSSSPLRT TSETDEKYAN VKWEELGFAL TPIDYMYVAK CRQGESFTQG
60 70 80 90 100
KIVPYGDISI SPCSPILNYG QGLFEGLKAY RTEDDRIRIF RPDQNALRMQ
110 120 130 140 150
TGAERLCMTP PTLEQFVEAV KQTVLANKKW VPPPGKGTLY IRPLLLGSGA
160 170 180 190 200
TLGVAPAPEY TFLIYASPVG DYHKVSSGLN LKVDHKYHRA HSGGTGGVKS
210 220 230 240 250
CTNYSPVVKS LLEAKSAGFS DVLFLDAATG RNIEELTACN IFIVKGNIVS
260 270 280 290 300
TPPTSGTILP GVTRKSISEL AHDIGYQVEE RDVSVDELLE AEEVFCTGTA
310 320 330 340 350
VVVKAVETVT FHDKKVKYRT GEAALSTKLH SMLTNIQMGV VEDKKGWMVD

IDPCQG
Length:356
Mass (Da):38,861
Last modified:October 1, 2000 - v1
Checksum:iC3F1A506BD3B82BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1406GKGTLY → VKELCI in CAA81418 (PubMed:8580968).Curated
Sequence conflicti145 – 1451L → P in AAM66943 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ312747 mRNA. Translation: CAC37393.1.
AC015445 Genomic DNA. Translation: AAF76437.1.
CP002684 Genomic DNA. Translation: AEE32516.1.
AY088621 mRNA. Translation: AAM66943.1.
Z26805 mRNA. Translation: CAA81418.1.
PIRiD96537.
RefSeqiNP_175431.1. NM_103897.3.
UniGeneiAt.10368.
At.67645.

Genome annotation databases

EnsemblPlantsiAT1G50110.1; AT1G50110.1; AT1G50110.
GeneIDi841433.
GrameneiAT1G50110.1; AT1G50110.1; AT1G50110.
KEGGiath:AT1G50110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ312747 mRNA. Translation: CAC37393.1.
AC015445 Genomic DNA. Translation: AAF76437.1.
CP002684 Genomic DNA. Translation: AEE32516.1.
AY088621 mRNA. Translation: AAM66943.1.
Z26805 mRNA. Translation: CAA81418.1.
PIRiD96537.
RefSeqiNP_175431.1. NM_103897.3.
UniGeneiAt.10368.
At.67645.

3D structure databases

ProteinModelPortaliQ9LPM9.
SMRiQ9LPM9. Positions 20-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G50110.1.

PTM databases

iPTMnetiQ9LPM9.

Proteomic databases

PaxDbiQ9LPM9.
PRIDEiQ9LPM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G50110.1; AT1G50110.1; AT1G50110.
GeneIDi841433.
GrameneiAT1G50110.1; AT1G50110.1; AT1G50110.
KEGGiath:AT1G50110.

Organism-specific databases

TAIRiAT1G50110.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
InParanoidiQ9LPM9.
KOiK00826.
OMAiKVKYRTG.
OrthoDBiEOG09360CSJ.
PhylomeDBiQ9LPM9.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00058.
UPA00048; UER00073.
UPA00049; UER00062.
BioCyciARA:AT1G50110-MONOMER.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ9LPM9.

Gene expression databases

GenevisibleiQ9LPM9. AT.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAT6_ARATH
AccessioniPrimary (citable) accession number: Q9LPM9
Secondary accession number(s): Q42148, Q8L961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.