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Protein

DNA replication licensing factor MCM2

Gene

MCM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells. May play a crucial role in the control of de-differentiation and cell proliferation processes required for lateral root formation. Is essential for embryo development. Is involved in the geminivirus mungbean yellow mosaic virus (MYMV) DNA replication, presumably in conjunction with other host factors.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri355 – 38127C4-typeSequence analysisAdd
BLAST
Nucleotide bindingi549 – 5568ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • regulation of cell proliferation Source: TAIR
  • regulation of root meristem growth Source: TAIR
  • root development Source: TAIR
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication, Host-virus interaction

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM2 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 2
Short name:
AtMCM2
Gene namesi
Name:MCM2
Ordered Locus Names:At1g44900
ORF Names:T12C22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G44900.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • MCM complex Source: InterPro
  • THO complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936DNA replication licensing factor MCM2PRO_0000425986Add
BLAST

Proteomic databases

PaxDbiQ9LPD9.
PRIDEiQ9LPD9.

PTM databases

iPTMnetiQ9LPD9.

Expressioni

Tissue specificityi

Expressed in root apical meristem, lateral root meristem primordia, leaf primordia, shoot apical meristem and flower buds.2 Publications

Gene expression databases

ExpressionAtlasiQ9LPD9. baseline and differential.
GenevisibleiQ9LPD9. AT.

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1 and the replication-associated protein of the geminivirus mungbean yellow mosaic virus (MYMV).2 Publications

Protein-protein interaction databases

BioGridi26281. 2 interactions.
IntActiQ9LPD9. 2 interactions.
MINTiMINT-6799282.
STRINGi3702.AT1G44900.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LPD9.
SMRiQ9LPD9. Positions 214-846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini499 – 705207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi681 – 6844Arginine finger

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 192141Asp-richAdd
BLAST
Compositional biasi56 – 6611Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri355 – 38127C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0477. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224124.
InParanoidiQ9LPD9.
OMAiKYDRIAH.
PhylomeDBiQ9LPD9.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF44. PTHR11630:SF44. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LPD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGENSDNEP SSPASPSSAG FNTDQLPIST SQNSENFSDE EEAAVDTQVI
60 70 80 90 100
RDEPDEAEDE EEEEGEDLFN DTFMNDYRKM DENDQYESNG IDDSVDDERD
110 120 130 140 150
LGQAMLDRRA ADADLDAREN RLANRKLPHL LHDNDSDDWN YRPSKRSRTT
160 170 180 190 200
VPPRGNGGDP DGNPPSSPGV SQPDISMTDQ TDDYQDEDDN DDEAEFEMYR
210 220 230 240 250
IQGTLREWVM RDEVRRFIAK KFKDFLLTYV KPKNENGDIE YVRLINEMVS
260 270 280 290 300
ANKCSLEIDY KEFIHVHPNI AIWLADAPQP VLEVMEEVSE KVIFDLHPNY
310 320 330 340 350
KNIHTKIYVR VTNLPVNDQI RNIRQIHLNT MIRIGGVVTR RSGVFPQLQQ
360 370 380 390 400
VKYDCNKCGA VLGPFFQNSY SEVKVGSCSE CQSKGPFTVN VEQTIYRNYQ
410 420 430 440 450
KLTIQESPGT VPAGRLPRHK EVILLNDLID CARPGEEIEV TGIYTNNFDL
460 470 480 490 500
SLNTKNGFPV FATVVEANYV TKKQDLFSAY KLTQEDKTQI EELSKDPRIV
510 520 530 540 550
ERIIKSIAPS IYGHEDIKTA LALAMFGGQE KNIKGKHRLR GDINVLLLGD
560 570 580 590 600
PGTAKSQFLK YVEKTGQRAV YTTGKGASAV GLTAAVHKDP VTREWTLEGG
610 620 630 640 650
ALVLADRGIC LIDEFDKMND QDRVSIHEAM EQQSISISKA GIVTSLQARC
660 670 680 690 700
SVIAAANPVG GRYDSSKSFA QNVELTDPIL SRFDILCVVK DVVDPVTDEM
710 720 730 740 750
LAEFVVNSHF KSQPKGGKME DSDPEDGIQG SSGSTDPEVL PQNLLKKYLT
760 770 780 790 800
YSKLYVFPKL GELDAKKLET VYANLRRESM NGQGVSIATR HLESMIRMSE
810 820 830 840 850
AHARMHLRQY VTEEDVNMAI RVLLDSFIST QKFGVQRTLR ESFKRYITYK
860 870 880 890 900
KDFNSLLLVL LKELVKNALK FEEIISGSNS GLPTIEVKIE ELQTKAKEYD
910 920 930
IADLRPFFSS TDFSKAHFEL DHGRGMIKCP KRLITW
Length:936
Mass (Da):105,584
Last modified:October 1, 2000 - v1
Checksum:iEDED5161964DBC69
GO

Sequence cautioni

The sequence AK227536 differs from that shown.Sequencing errors.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020576 Genomic DNA. Translation: AAF78275.1.
CP002684 Genomic DNA. Translation: AEE32060.1.
AK227536 mRNA. No translation available.
Y08301 mRNA. Translation: CAA69609.1.
PIRiE96508.
RefSeqiNP_175112.2. NM_103572.4. [Q9LPD9-1]
UniGeneiAt.5390.

Genome annotation databases

EnsemblPlantsiAT1G44900.1; AT1G44900.1; AT1G44900. [Q9LPD9-1]
GeneIDi841056.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020576 Genomic DNA. Translation: AAF78275.1.
CP002684 Genomic DNA. Translation: AEE32060.1.
AK227536 mRNA. No translation available.
Y08301 mRNA. Translation: CAA69609.1.
PIRiE96508.
RefSeqiNP_175112.2. NM_103572.4. [Q9LPD9-1]
UniGeneiAt.5390.

3D structure databases

ProteinModelPortaliQ9LPD9.
SMRiQ9LPD9. Positions 214-846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26281. 2 interactions.
IntActiQ9LPD9. 2 interactions.
MINTiMINT-6799282.
STRINGi3702.AT1G44900.1.

PTM databases

iPTMnetiQ9LPD9.

Proteomic databases

PaxDbiQ9LPD9.
PRIDEiQ9LPD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G44900.1; AT1G44900.1; AT1G44900. [Q9LPD9-1]
GeneIDi841056.

Organism-specific databases

TAIRiAT1G44900.

Phylogenomic databases

eggNOGiKOG0477. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224124.
InParanoidiQ9LPD9.
OMAiKYDRIAH.
PhylomeDBiQ9LPD9.

Enzyme and pathway databases

ReactomeiR-ATH-68867. Assembly of the pre-replicative complex.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69052. Switching of origins to a post-replicative state.
R-ATH-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiQ9LPD9.

Gene expression databases

ExpressionAtlasiQ9LPD9. baseline and differential.
GenevisibleiQ9LPD9. AT.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF44. PTHR11630:SF44. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A.thaliana mRNA for MCM2-related protein."
    Callard P., Axelos M.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 446-936.
    Strain: cv. Columbia.
  5. "Genome-wide analysis of the core DNA replication machinery in the higher plants Arabidopsis and rice."
    Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.
    Plant Physiol. 144:1697-1714(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1."
    Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G., Witters E., Inze D., De Veylder L.
    EMBO J. 27:1840-1851(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH ETG1.
  7. "The Arabidopsis MCM2 gene is essential to embryo development and its over-expression alters root meristem function."
    Ni D.A., Sozzani R., Blanchet S., Domenichini S., Reuzeau C., Cella R., Bergounioux C., Raynaud C.
    New Phytol. 184:311-322(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. "Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants."
    Shultz R.W., Lee T.J., Allen G.C., Thompson W.F., Hanley-Bowdoin L.
    Plant Physiol. 150:658-669(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "Arabidopsis thaliana MCM2 plays role(s) in mungbean yellow mosaic India virus (MYMIV) DNA replication."
    Suyal G., Mukherjee S.K., Srivastava P.S., Choudhury N.R.
    Arch. Virol. 158:981-992(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE GEMINIVIRUS MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) REPLICATION INITIATOR PROTEIN.

Entry informationi

Entry nameiMCM2_ARATH
AccessioniPrimary (citable) accession number: Q9LPD9
Secondary accession number(s): Q96275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2000
Last modified: January 20, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Over-expression of MCM2 strongly reduces plant and cell sizes and inhibits endoreduplication.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.