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Protein

DNA replication licensing factor MCM2

Gene

MCM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable component of the MCM2-7 complex (MCM complex) that may function as a DNA helicase and which is essential to undergo a single round of replication initiation and elongation per cell cycle in eukaryotic cells. May play a crucial role in the control of de-differentiation and cell proliferation processes required for lateral root formation. Is essential for embryo development. Is involved in the geminivirus mungbean yellow mosaic virus (MYMV) DNA replication, presumably in conjunction with other host factors.2 Publications

Miscellaneous

Over-expression of MCM2 strongly reduces plant and cell sizes and inhibits endoreduplication.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri355 – 381C4-typeSequence analysisAdd BLAST27
Nucleotide bindingi549 – 556ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • negative regulation of DNA helicase activity Source: InterPro
  • regulation of cell proliferation Source: TAIR
  • regulation of root meristem growth Source: TAIR
  • root development Source: TAIR
  • viral process Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication, Host-virus interaction
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex
R-ATH-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM2 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 2
Short name:
AtMCM2
Gene namesi
Name:MCM2
Ordered Locus Names:At1g44900
ORF Names:T12C22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G44900
TAIRilocus:2028240 AT1G44900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004259861 – 936DNA replication licensing factor MCM2Add BLAST936

Proteomic databases

PaxDbiQ9LPD9

PTM databases

iPTMnetiQ9LPD9

Expressioni

Tissue specificityi

Expressed in root apical meristem, lateral root meristem primordia, leaf primordia, shoot apical meristem and flower buds.2 Publications

Gene expression databases

ExpressionAtlasiQ9LPD9 baseline and differential
GenevisibleiQ9LPD9 AT

Interactioni

Subunit structurei

Component of the minichromosome maintenance (MCM) complex, a heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7. Interacts with ETG1 and the replication-associated protein of the geminivirus mungbean yellow mosaic virus (MYMV).2 Publications

Protein-protein interaction databases

BioGridi26281, 2 interactors
IntActiQ9LPD9, 2 interactors
MINTiQ9LPD9
STRINGi3702.AT1G44900.1

Structurei

3D structure databases

ProteinModelPortaliQ9LPD9
SMRiQ9LPD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini499 – 705MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi681 – 684Arginine finger4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi52 – 192Asp-richAdd BLAST141
Compositional biasi56 – 66Poly-GluAdd BLAST11

Sequence similaritiesi

Belongs to the MCM family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri355 – 381C4-typeSequence analysisAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0477 Eukaryota
COG1241 LUCA
HOGENOMiHOG000224124
InParanoidiQ9LPD9
KOiK02540
OMAiKYDCVKC
OrthoDBiEOG0936020T
PhylomeDBiQ9LPD9

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008045 MCM2
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF44 PTHR11630:SF44, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF12619 MCM2_N, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01658 MCMPROTEIN2
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LPD9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGENSDNEP SSPASPSSAG FNTDQLPIST SQNSENFSDE EEAAVDTQVI
60 70 80 90 100
RDEPDEAEDE EEEEGEDLFN DTFMNDYRKM DENDQYESNG IDDSVDDERD
110 120 130 140 150
LGQAMLDRRA ADADLDAREN RLANRKLPHL LHDNDSDDWN YRPSKRSRTT
160 170 180 190 200
VPPRGNGGDP DGNPPSSPGV SQPDISMTDQ TDDYQDEDDN DDEAEFEMYR
210 220 230 240 250
IQGTLREWVM RDEVRRFIAK KFKDFLLTYV KPKNENGDIE YVRLINEMVS
260 270 280 290 300
ANKCSLEIDY KEFIHVHPNI AIWLADAPQP VLEVMEEVSE KVIFDLHPNY
310 320 330 340 350
KNIHTKIYVR VTNLPVNDQI RNIRQIHLNT MIRIGGVVTR RSGVFPQLQQ
360 370 380 390 400
VKYDCNKCGA VLGPFFQNSY SEVKVGSCSE CQSKGPFTVN VEQTIYRNYQ
410 420 430 440 450
KLTIQESPGT VPAGRLPRHK EVILLNDLID CARPGEEIEV TGIYTNNFDL
460 470 480 490 500
SLNTKNGFPV FATVVEANYV TKKQDLFSAY KLTQEDKTQI EELSKDPRIV
510 520 530 540 550
ERIIKSIAPS IYGHEDIKTA LALAMFGGQE KNIKGKHRLR GDINVLLLGD
560 570 580 590 600
PGTAKSQFLK YVEKTGQRAV YTTGKGASAV GLTAAVHKDP VTREWTLEGG
610 620 630 640 650
ALVLADRGIC LIDEFDKMND QDRVSIHEAM EQQSISISKA GIVTSLQARC
660 670 680 690 700
SVIAAANPVG GRYDSSKSFA QNVELTDPIL SRFDILCVVK DVVDPVTDEM
710 720 730 740 750
LAEFVVNSHF KSQPKGGKME DSDPEDGIQG SSGSTDPEVL PQNLLKKYLT
760 770 780 790 800
YSKLYVFPKL GELDAKKLET VYANLRRESM NGQGVSIATR HLESMIRMSE
810 820 830 840 850
AHARMHLRQY VTEEDVNMAI RVLLDSFIST QKFGVQRTLR ESFKRYITYK
860 870 880 890 900
KDFNSLLLVL LKELVKNALK FEEIISGSNS GLPTIEVKIE ELQTKAKEYD
910 920 930
IADLRPFFSS TDFSKAHFEL DHGRGMIKCP KRLITW
Length:936
Mass (Da):105,584
Last modified:October 1, 2000 - v1
Checksum:iEDED5161964DBC69
GO

Sequence cautioni

The sequence AK227536 differs from that shown. Sequencing errors.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020576 Genomic DNA Translation: AAF78275.1
CP002684 Genomic DNA Translation: AEE32060.1
AK227536 mRNA No translation available.
Y08301 mRNA Translation: CAA69609.1
PIRiE96508
RefSeqiNP_175112.2, NM_103572.5 [Q9LPD9-1]
UniGeneiAt.5390

Genome annotation databases

EnsemblPlantsiAT1G44900.1; AT1G44900.1; AT1G44900 [Q9LPD9-1]
GeneIDi841056
GrameneiAT1G44900.1; AT1G44900.1; AT1G44900 [Q9LPD9-1]
KEGGiath:AT1G44900

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMCM2_ARATH
AccessioniPrimary (citable) accession number: Q9LPD9
Secondary accession number(s): Q96275
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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