Reviewed, UniProtKB/Swiss-Prot Q9LPC6 (ARK2_ARATH)

Names and origin

Protein names

Recommended name:
    Armadillo repeat-containing kinesin-like protein 2

Gene names

Name:	ARK2
Ordered Locus Names:	At1g01950
ORF Names:	F22M8.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the control of epidermal-cell morphogenesis in roots and helical growth of roots by promoting microtubule depolymerization and limiting the accumulation of endoplasmic microtubules. Seems to be involved in the control of cell-file rotation (or twisting). Ref.1
Subunit structure	Interacts (via C-terminus) with NEK5. Ref.1
Tissue specificity	Expressed in the basal regions and petioles of immature leaves and in the root elongation zone. Ref.1
Sequence similarities	Belongs to the kinesin-like protein family. Contains 4 ARM repeats. Contains 1 kinesin-motor domain.
Sequence caution	The sequence AAF76473.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	microtubule-based movement Inferred from electronic annotation. Source: InterPro
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LPC6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 894	894	Armadillo repeat-containing kinesin-like protein 2		PRO_0000342331
Regions
	Domain	57 – 333	277	Kinesin-motor
	Repeat	626 – 665	40	ARM 1
	Repeat	667 – 707	41	ARM 2
	Repeat	709 – 749	41	ARM 3
	Repeat	751 – 790	40	ARM 4
	Nucleotide binding	145 – 152	8	ATP Potential
	Coiled coil	413 – 592	180	Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: 7902A8AA2A1F3829	FASTA	894	98,423
	10 20 30 40 50 60 MAMASSRNGA VRGSMRPVSG ANSSNLRSSS FKSRIPSSAP APRRSSSASI GAADNGVPGR 70 80 90 100 110 120 VRVAVRLRPR NADESVADAD FADCVELQPE LKRLKLRKNN WDTETYEFDE VLTEAASQKR 130 140 150 160 170 180 VYEVVAKPVV ESVLEGYNGT VMAYGQTGTG KTFTLGRLGD EDTAARGIMV RSMEDIIGGT 190 200 210 220 230 240 SLDTDSISVS YLQLYMETIQ DLLDPTNDNI AIVEDPRTGD VSLPGATHVE IRNQQNFLEL 250 260 270 280 290 300 LQLGETHRVA ANTKLNTESS RSHAILMVHV KRSVVENEFP VSNEMESSSH FVRPSKPLVR 310 320 330 340 350 360 RSKLVLVDLA GSERVHKSGS EGHMLEEAKS INLSLSALGK CINAIAENSP HVPLRDSKLT 370 380 390 400 410 420 RLLRDSFGGT ARTSLIVTIG PSPRHRGETT STILFGQRAM KVENMLKIKE EFDYKSLSKK 430 440 450 460 470 480 LEVQLDKVIA ENERQLKAFD DDVERINRQA QNRISEVEKN FAEALEKEKL KCQMEYMESV 490 500 510 520 530 540 KKLEEKLISN QRNHENGKRN GEVNGVVTAS EFTRLKESLE NEMKLRKSAE EEVSKVKSQS 550 560 570 580 590 600 TLKTRSGEGE DAGITRLQKL LEDEALQKKK LEEEVTILRS QLVQLTFEAD QMRRCLDRGA 610 620 630 640 650 660 PGNSYSGTDS LPSRHSQARE SVNGQKAPFA TLCEQVGLQK ILQLLESDDA NIRIHAVKVV 670 680 690 700 710 720 ANLAAEEANQ EKIVEAGGLT SLLMLLRSYE DETVRRVAAG AIANLAMNEV SQQLIVDQGG 730 740 750 760 770 780 ISLLSLTAAD AEDPQTLRMV AGAIANLCGN DKLQARLWSD GGIKALLGMV RCGHPDVLAQ 790 800 810 820 830 840 VARGIANFAK CESRATTQGV KSGRSLLIED GALPWIVQHA NDEAAPIRRH IELALCHLAQ 850 860 870 880 890 HEVNAKEMIS GGALWELVRI SKECSREDIR SLAHRTLSSS PVFRSEIRRL GIQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal-cell morphogenesis in Arabidopsis." Sakai T., van der Honing H., Nishioka M., Uehara Y., Takahashi M., Fujisawa N., Saji K., Seki M., Shinozaki K., Jones M.A., Smirnoff N., Okada K., Wasteneys G.O. Plant J. 53:157-171(2008) [PubMed: 17971038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH NEK5. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 684-894. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB290929 mRNA. Translation: BAF95586.1. AC020622 Genomic DNA. Translation: AAF76473.1. Sequence problems. AK229671 mRNA. Translation: BAF01514.1.
IPI	IPI00527888.
PIR	D86151.
RefSeq	NP_171697.3.
UniGene	At.66839
3D structure databases
HSSP	HSSP built from PDB template 1T5C based on UniProtKB Q02224.
ModBase	Search...
Proteomic databases
PRIDE	Q9LPC6.
Genome annotation databases
GeneID	839306.
GenomeReviews	Gene locus AT1G01950 in contig CT485782_GR.
KEGG	ath:AT1G01950.
Organism-specific databases
GeneFarm	2958. 282.
TAIR	At1g01950.
Phylogenomic databases
OMA	SEGHMLE
Gene expression databases
Genevestigator	Q9LPC6.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR001752. Kinesin_motor. IPR019821. Kinesin_motor_CS. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit. G3DSA:3.40.850.10. kinesin_motor. 1 hit.
Pfam	PF00514. Arm. 4 hits. PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00185. ARM. 4 hits. SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS50176. ARM_REPEAT. 2 hits. PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARK2_ARATH

Accession

Primary (citable) accession number: Q9LPC6
Secondary accession number(s): A9CP40, Q0WMY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	November 24, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)