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Q9LNW3

- P2C03_ARATH

UniProt

Q9LNW3 - P2C03_ARATH

Protein

Protein phosphatase 2C 3

Gene

AIP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in the negative regulation of the K+ potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23).1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi162 – 1621Manganese 1By similarity
    Metal bindingi162 – 1621Manganese 2By similarity
    Metal bindingi163 – 1631Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi339 – 3391Manganese 2By similarity
    Metal bindingi424 – 4241Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. potassium channel activity Source: UniProtKB-KW
    3. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. negative regulation of abscisic acid-activated signaling pathway Source: TAIR
    2. negative regulation of seed dormancy process Source: TAIR
    3. positive regulation of gibberellic acid mediated signaling pathway Source: TAIR
    4. positive regulation of seed germination Source: TAIR
    5. protein dephosphorylation Source: InterPro
    6. release of seed from dormancy Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Ion channel, Potassium channel, Protein phosphatase

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, Potassium

    Enzyme and pathway databases

    BioCyciARA:AT1G07430-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 3 (EC:3.1.3.16)
    Short name:
    AtPP2C03
    Alternative name(s):
    Protein AKT1-INTERACTING 1
    Protein phosphatase 2C AIP1
    Short name:
    PP2C AIP1
    Gene namesi
    Name:AIP1
    Ordered Locus Names:At1g07430
    ORF Names:F22G5.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G07430.

    Subcellular locationi

    Cell membrane Curated
    Note: Probably associated to the plasma membrane when interacting with AKT1 and CIPK23.

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Protein phosphatase 2C 3PRO_0000344523Add
    BLAST

    Proteomic databases

    PRIDEiQ9LNW3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LNW3.

    Interactioni

    Subunit structurei

    Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1 and CIPK23.1 Publication

    Protein-protein interaction databases

    BioGridi22496. 12 interactions.
    DIPiDIP-40200N.
    IntActiQ9LNW3. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LNW3.
    SMRiQ9LNW3. Positions 119-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini119 – 426308PP2C-likeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi71 – 9929Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiQ9LNW3.
    KOiK14497.
    OMAiSANCRCE.
    PhylomeDBiQ9LNW3.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LNW3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADICYEDET SACESRPLWS SRKWRIGVQR FRMSPSEMNP TASTTEEEDK    50
    SEGIYNKRNK QEEYDFMNCA SSSPSQSSPE EESVSLEDSD VSISDGNSSV 100
    NDVAVIPSKK TVKETDLRPR YGVASVCGRR RDMEDAVALH PSFVRKQTEF 150
    SRTRWHYFGV YDGHGCSHVA ARCKERLHEL VQEEALSDKK EEWKKMMERS 200
    FTRMDKEVVR WGETVMSANC RCELQTPDCD AVGSTAVVSV ITPEKIIVAN 250
    CGDSRAVLCR NGKAVPLSTD HKPDRPDELD RIQEAGGRVI YWDGARVLGV 300
    LAMSRAIGDN YLKPYVTSEP EVTVTDRTEE DEFLILATDG LWDVVTNEAA 350
    CTMVRMCLNR KSGRGRRRGE TQTPGRRSEE EGKEEEEKVV GSRKNGKRGE 400
    ITDKACTEAS VLLTKLALAK HSSDNVSVVV IDLRRRRKRH VA 442
    Length:442
    Mass (Da):49,763
    Last modified:October 1, 2000 - v1
    Checksum:i892DB70AB83312AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti253 – 2531D → G in AAM61361. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC022464 Genomic DNA. Translation: AAF79555.1.
    CP002684 Genomic DNA. Translation: AEE28124.1.
    AY070089 mRNA. Translation: AAL49783.1.
    AY091341 mRNA. Translation: AAM14280.1.
    AY084794 mRNA. Translation: AAM61361.1.
    PIRiB86209.
    RefSeqiNP_172223.1. NM_100617.2.
    UniGeneiAt.28399.

    Genome annotation databases

    EnsemblPlantsiAT1G07430.1; AT1G07430.1; AT1G07430.
    GeneIDi837255.
    KEGGiath:AT1G07430.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC022464 Genomic DNA. Translation: AAF79555.1 .
    CP002684 Genomic DNA. Translation: AEE28124.1 .
    AY070089 mRNA. Translation: AAL49783.1 .
    AY091341 mRNA. Translation: AAM14280.1 .
    AY084794 mRNA. Translation: AAM61361.1 .
    PIRi B86209.
    RefSeqi NP_172223.1. NM_100617.2.
    UniGenei At.28399.

    3D structure databases

    ProteinModelPortali Q9LNW3.
    SMRi Q9LNW3. Positions 119-434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22496. 12 interactions.
    DIPi DIP-40200N.
    IntActi Q9LNW3. 3 interactions.

    Proteomic databases

    PRIDEi Q9LNW3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G07430.1 ; AT1G07430.1 ; AT1G07430 .
    GeneIDi 837255.
    KEGGi ath:AT1G07430.

    Organism-specific databases

    TAIRi AT1G07430.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi Q9LNW3.
    KOi K14497.
    OMAi SANCRCE.
    PhylomeDBi Q9LNW3.

    Enzyme and pathway databases

    BioCyci ARA:AT1G07430-MONOMER.

    Gene expression databases

    Genevestigatori Q9LNW3.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Plant PP2C phosphatases: emerging functions in stress signaling."
      Schweighofer A., Hirt H., Meskiene I.
      Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "A protein phosphorylation/dephosphorylation network regulates a plant potassium channel."
      Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S.
      Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AKT1 AND CIPK23.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C03_ARATH
    AccessioniPrimary (citable) accession number: Q9LNW3
    Secondary accession number(s): Q8LFK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3