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Q9LNW3 (P2C03_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C 3

Short name=AtPP2C03
EC=3.1.3.16
Alternative name(s):
Protein AKT1-INTERACTING 1
Protein phosphatase 2C AIP1
Short name=PP2C AIP1
Gene names
Name:AIP1
Ordered Locus Names:At1g07430
ORF Names:F22G5.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the negative regulation of the K+ potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23). Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1 and CIPK23. Ref.6

Subcellular location

Cell membrane Probable. Note: Probably associated to the plasma membrane when interacting with AKT1 and CIPK23.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Protein phosphatase 2C 3
PRO_0000344523

Regions

Domain119 – 426308PP2C-like
Compositional bias71 – 9929Ser-rich

Sites

Metal binding1621Manganese 1 By similarity
Metal binding1621Manganese 2 By similarity
Metal binding1631Manganese 1; via carbonyl oxygen By similarity
Metal binding3391Manganese 2 By similarity
Metal binding4241Manganese 2 By similarity

Experimental info

Sequence conflict2531D → G in AAM61361. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9LNW3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 892DB70AB83312AD

FASTA44249,763
        10         20         30         40         50         60 
MADICYEDET SACESRPLWS SRKWRIGVQR FRMSPSEMNP TASTTEEEDK SEGIYNKRNK 

        70         80         90        100        110        120 
QEEYDFMNCA SSSPSQSSPE EESVSLEDSD VSISDGNSSV NDVAVIPSKK TVKETDLRPR 

       130        140        150        160        170        180 
YGVASVCGRR RDMEDAVALH PSFVRKQTEF SRTRWHYFGV YDGHGCSHVA ARCKERLHEL 

       190        200        210        220        230        240 
VQEEALSDKK EEWKKMMERS FTRMDKEVVR WGETVMSANC RCELQTPDCD AVGSTAVVSV 

       250        260        270        280        290        300 
ITPEKIIVAN CGDSRAVLCR NGKAVPLSTD HKPDRPDELD RIQEAGGRVI YWDGARVLGV 

       310        320        330        340        350        360 
LAMSRAIGDN YLKPYVTSEP EVTVTDRTEE DEFLILATDG LWDVVTNEAA CTMVRMCLNR 

       370        380        390        400        410        420 
KSGRGRRRGE TQTPGRRSEE EGKEEEEKVV GSRKNGKRGE ITDKACTEAS VLLTKLALAK 

       430        440 
HSSDNVSVVV IDLRRRRKRH VA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Plant PP2C phosphatases: emerging functions in stress signaling."
Schweighofer A., Hirt H., Meskiene I.
Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"A protein phosphorylation/dephosphorylation network regulates a plant potassium channel."
Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S.
Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AKT1 AND CIPK23.
[7]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC022464 Genomic DNA. Translation: AAF79555.1.
CP002684 Genomic DNA. Translation: AEE28124.1.
AY070089 mRNA. Translation: AAL49783.1.
AY091341 mRNA. Translation: AAM14280.1.
AY084794 mRNA. Translation: AAM61361.1.
PIRB86209.
RefSeqNP_172223.1. NM_100617.2.
UniGeneAt.28399.

3D structure databases

ProteinModelPortalQ9LNW3.
SMRQ9LNW3. Positions 119-434.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22496. 12 interactions.
DIPDIP-40200N.
IntActQ9LNW3. 3 interactions.

Proteomic databases

PRIDEQ9LNW3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G07430.1; AT1G07430.1; AT1G07430.
GeneID837255.
KEGGath:AT1G07430.

Organism-specific databases

TAIRAT1G07430.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233896.
InParanoidQ9LNW3.
KOK14497.
OMASANCRCE.
PhylomeDBQ9LNW3.

Enzyme and pathway databases

BioCycARA:AT1G07430-MONOMER.

Gene expression databases

GenevestigatorQ9LNW3.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 2 hits.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C03_ARATH
AccessionPrimary (citable) accession number: Q9LNW3
Secondary accession number(s): Q8LFK5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names