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Protein

Protein phosphatase 2C 3

Gene

AIP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the negative regulation of the K+ potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23).1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi162Manganese 1By similarity1
Metal bindingi162Manganese 2By similarity1
Metal bindingi163Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi339Manganese 2By similarity1
Metal bindingi424Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  • negative regulation of seed dormancy process Source: TAIR
  • positive regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • positive regulation of seed germination Source: TAIR
  • release of seed from dormancy Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ion channel, Potassium channel, Protein phosphatase

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 3 (EC:3.1.3.16)
Short name:
AtPP2C03
Alternative name(s):
Protein AKT1-INTERACTING 1
Protein phosphatase 2C AIP1
Short name:
PP2C AIP1
Gene namesi
Name:AIP1
Ordered Locus Names:At1g07430
ORF Names:F22G5.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07430.

Subcellular locationi

  • Cell membrane Curated

  • Note: Probably associated to the plasma membrane when interacting with AKT1 and CIPK23.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003445231 – 442Protein phosphatase 2C 3Add BLAST442

Proteomic databases

PaxDbiQ9LNW3.

PTM databases

iPTMnetiQ9LNW3.

Expressioni

Gene expression databases

GenevisibleiQ9LNW3. AT.

Interactioni

Subunit structurei

Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1 and CIPK23.1 Publication

Protein-protein interaction databases

BioGridi22496. 12 interactors.
DIPiDIP-40200N.
IntActiQ9LNW3. 3 interactors.
STRINGi3702.AT1G07430.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LNW3.
SMRiQ9LNW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 433PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST314

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi71 – 99Ser-richAdd BLAST29

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9LNW3.
KOiK14497.
OMAiMSANCRC.
OrthoDBiEOG09360IAR.
PhylomeDBiQ9LNW3.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LNW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADICYEDET SACESRPLWS SRKWRIGVQR FRMSPSEMNP TASTTEEEDK
60 70 80 90 100
SEGIYNKRNK QEEYDFMNCA SSSPSQSSPE EESVSLEDSD VSISDGNSSV
110 120 130 140 150
NDVAVIPSKK TVKETDLRPR YGVASVCGRR RDMEDAVALH PSFVRKQTEF
160 170 180 190 200
SRTRWHYFGV YDGHGCSHVA ARCKERLHEL VQEEALSDKK EEWKKMMERS
210 220 230 240 250
FTRMDKEVVR WGETVMSANC RCELQTPDCD AVGSTAVVSV ITPEKIIVAN
260 270 280 290 300
CGDSRAVLCR NGKAVPLSTD HKPDRPDELD RIQEAGGRVI YWDGARVLGV
310 320 330 340 350
LAMSRAIGDN YLKPYVTSEP EVTVTDRTEE DEFLILATDG LWDVVTNEAA
360 370 380 390 400
CTMVRMCLNR KSGRGRRRGE TQTPGRRSEE EGKEEEEKVV GSRKNGKRGE
410 420 430 440
ITDKACTEAS VLLTKLALAK HSSDNVSVVV IDLRRRRKRH VA
Length:442
Mass (Da):49,763
Last modified:October 1, 2000 - v1
Checksum:i892DB70AB83312AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253D → G in AAM61361 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022464 Genomic DNA. Translation: AAF79555.1.
CP002684 Genomic DNA. Translation: AEE28124.1.
AY070089 mRNA. Translation: AAL49783.1.
AY091341 mRNA. Translation: AAM14280.1.
AY084794 mRNA. Translation: AAM61361.1.
PIRiB86209.
RefSeqiNP_172223.1. NM_100617.3.
UniGeneiAt.28399.

Genome annotation databases

EnsemblPlantsiAT1G07430.1; AT1G07430.1; AT1G07430.
GeneIDi837255.
GrameneiAT1G07430.1; AT1G07430.1; AT1G07430.
KEGGiath:AT1G07430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022464 Genomic DNA. Translation: AAF79555.1.
CP002684 Genomic DNA. Translation: AEE28124.1.
AY070089 mRNA. Translation: AAL49783.1.
AY091341 mRNA. Translation: AAM14280.1.
AY084794 mRNA. Translation: AAM61361.1.
PIRiB86209.
RefSeqiNP_172223.1. NM_100617.3.
UniGeneiAt.28399.

3D structure databases

ProteinModelPortaliQ9LNW3.
SMRiQ9LNW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22496. 12 interactors.
DIPiDIP-40200N.
IntActiQ9LNW3. 3 interactors.
STRINGi3702.AT1G07430.1.

PTM databases

iPTMnetiQ9LNW3.

Proteomic databases

PaxDbiQ9LNW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07430.1; AT1G07430.1; AT1G07430.
GeneIDi837255.
GrameneiAT1G07430.1; AT1G07430.1; AT1G07430.
KEGGiath:AT1G07430.

Organism-specific databases

TAIRiAT1G07430.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9LNW3.
KOiK14497.
OMAiMSANCRC.
OrthoDBiEOG09360IAR.
PhylomeDBiQ9LNW3.

Miscellaneous databases

PROiQ9LNW3.

Gene expression databases

GenevisibleiQ9LNW3. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C03_ARATH
AccessioniPrimary (citable) accession number: Q9LNW3
Secondary accession number(s): Q8LFK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.