Q9LNW3 (P2C03_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 3
Short name=AtPP2C03
EC=3.1.3.16

Alternative name(s):
Protein AKT1-INTERACTING 1
Protein phosphatase 2C AIP1
Short name=PP2C AIP1

Gene names

Name:	AIP1
Ordered Locus Names:	At1g07430
ORF Names:	F22G5.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the negative regulation of the K+ potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23). Ref.6
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1 and CIPK23. Ref.6
Subcellular location	Cell membrane Probable. Note: Probably associated to the plasma membrane when interacting with AKT1 and CIPK23.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Membrane
Ligand	Magnesium Manganese Metal-binding Potassium
Molecular function	Hydrolase Ion channel Potassium channel Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium channel activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Protein phosphatase 2C 3		PRO_0000344523
Regions
	Domain	119 – 426	308	PP2C-like
	Compositional bias	71 – 99	29	Ser-rich
Sites
	Metal binding	162	1	Manganese 1 By similarity
	Metal binding	162	1	Manganese 2 By similarity
	Metal binding	163	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	339	1	Manganese 2 By similarity
	Metal binding	424	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	253	1	D → G in AAM61361. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9LNW3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 892DB70AB83312AD	FASTA	442	49,763
	10 20 30 40 50 60 MADICYEDET SACESRPLWS SRKWRIGVQR FRMSPSEMNP TASTTEEEDK SEGIYNKRNK 70 80 90 100 110 120 QEEYDFMNCA SSSPSQSSPE EESVSLEDSD VSISDGNSSV NDVAVIPSKK TVKETDLRPR 130 140 150 160 170 180 YGVASVCGRR RDMEDAVALH PSFVRKQTEF SRTRWHYFGV YDGHGCSHVA ARCKERLHEL 190 200 210 220 230 240 VQEEALSDKK EEWKKMMERS FTRMDKEVVR WGETVMSANC RCELQTPDCD AVGSTAVVSV 250 260 270 280 290 300 ITPEKIIVAN CGDSRAVLCR NGKAVPLSTD HKPDRPDELD RIQEAGGRVI YWDGARVLGV 310 320 330 340 350 360 LAMSRAIGDN YLKPYVTSEP EVTVTDRTEE DEFLILATDG LWDVVTNEAA CTMVRMCLNR 370 380 390 400 410 420 KSGRGRRRGE TQTPGRRSEE EGKEEEEKVV GSRKNGKRGE ITDKACTEAS VLLTKLALAK 430 440 HSSDNVSVVV IDLRRRRKRH VA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Plant PP2C phosphatases: emerging functions in stress signaling." Schweighofer A., Hirt H., Meskiene I. Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"A protein phosphorylation/dephosphorylation network regulates a plant potassium channel." Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S. Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AKT1 AND CIPK23.
[7]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC022464 Genomic DNA. Translation: AAF79555.1. CP002684 Genomic DNA. Translation: AEE28124.1. AY070089 mRNA. Translation: AAL49783.1. AY091341 mRNA. Translation: AAM14280.1. AY084794 mRNA. Translation: AAM61361.1.
IPI	IPI00521891.
PIR	B86209.
RefSeq	NP_172223.1. NM_100617.2.
UniGene	At.28399.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q9LNW3.
SMR	Q9LNW3. Positions 119-434.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-40200N.
IntAct	Q9LNW3. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G07430.1; AT1G07430.1; AT1G07430.
GeneID	837255.
KEGG	ath:AT1G07430.
Organism-specific databases
TAIR	At1g07430.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q9LNW3.
KO	K14497.
OMA	SANCRCE.
PhylomeDB	Q9LNW3.
ProtClustDB	CLSN2682567.
Gene expression databases
Genevestigator	Q9LNW3.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C03_ARATH

Accession

Primary (citable) accession number: Q9LNW3
Secondary accession number(s): Q8LFK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program