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Q9LNW3

- P2C03_ARATH

UniProt

Q9LNW3 - P2C03_ARATH

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Protein

Protein phosphatase 2C 3

Gene
AIP1, At1g07430, F22G5.22
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the negative regulation of the K+ potassium channel AKT1 by its dephosphorylation, antagonistically to CIPK proteins (e.g. CIPK23).1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621Manganese 1 By similarity
Metal bindingi162 – 1621Manganese 2 By similarity
Metal bindingi163 – 1631Manganese 1; via carbonyl oxygen By similarity
Metal bindingi339 – 3391Manganese 2 By similarity
Metal bindingi424 – 4241Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. potassium channel activity Source: UniProtKB-KW
  3. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  2. negative regulation of seed dormancy process Source: TAIR
  3. positive regulation of gibberellic acid mediated signaling pathway Source: TAIR
  4. positive regulation of seed germination Source: TAIR
  5. protein dephosphorylation Source: InterPro
  6. release of seed from dormancy Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ion channel, Potassium channel, Protein phosphatase

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT1G07430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 3 (EC:3.1.3.16)
Short name:
AtPP2C03
Alternative name(s):
Protein AKT1-INTERACTING 1
Protein phosphatase 2C AIP1
Short name:
PP2C AIP1
Gene namesi
Name:AIP1
Ordered Locus Names:At1g07430
ORF Names:F22G5.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G07430.

Subcellular locationi

Cell membrane Inferred
Note: Probably associated to the plasma membrane when interacting with AKT1 and CIPK23.

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Protein phosphatase 2C 3PRO_0000344523Add
BLAST

Proteomic databases

PRIDEiQ9LNW3.

Expressioni

Gene expression databases

GenevestigatoriQ9LNW3.

Interactioni

Subunit structurei

Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with AKT1 and CIPK23.1 Publication

Protein-protein interaction databases

BioGridi22496. 12 interactions.
DIPiDIP-40200N.
IntActiQ9LNW3. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9LNW3.
SMRiQ9LNW3. Positions 119-434.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 426308PP2C-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 9929Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LNW3.
KOiK14497.
OMAiSANCRCE.
PhylomeDBiQ9LNW3.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LNW3-1 [UniParc]FASTAAdd to Basket

« Hide

MADICYEDET SACESRPLWS SRKWRIGVQR FRMSPSEMNP TASTTEEEDK    50
SEGIYNKRNK QEEYDFMNCA SSSPSQSSPE EESVSLEDSD VSISDGNSSV 100
NDVAVIPSKK TVKETDLRPR YGVASVCGRR RDMEDAVALH PSFVRKQTEF 150
SRTRWHYFGV YDGHGCSHVA ARCKERLHEL VQEEALSDKK EEWKKMMERS 200
FTRMDKEVVR WGETVMSANC RCELQTPDCD AVGSTAVVSV ITPEKIIVAN 250
CGDSRAVLCR NGKAVPLSTD HKPDRPDELD RIQEAGGRVI YWDGARVLGV 300
LAMSRAIGDN YLKPYVTSEP EVTVTDRTEE DEFLILATDG LWDVVTNEAA 350
CTMVRMCLNR KSGRGRRRGE TQTPGRRSEE EGKEEEEKVV GSRKNGKRGE 400
ITDKACTEAS VLLTKLALAK HSSDNVSVVV IDLRRRRKRH VA 442
Length:442
Mass (Da):49,763
Last modified:October 1, 2000 - v1
Checksum:i892DB70AB83312AD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531D → G in AAM61361. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC022464 Genomic DNA. Translation: AAF79555.1.
CP002684 Genomic DNA. Translation: AEE28124.1.
AY070089 mRNA. Translation: AAL49783.1.
AY091341 mRNA. Translation: AAM14280.1.
AY084794 mRNA. Translation: AAM61361.1.
PIRiB86209.
RefSeqiNP_172223.1. NM_100617.2.
UniGeneiAt.28399.

Genome annotation databases

EnsemblPlantsiAT1G07430.1; AT1G07430.1; AT1G07430.
GeneIDi837255.
KEGGiath:AT1G07430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC022464 Genomic DNA. Translation: AAF79555.1 .
CP002684 Genomic DNA. Translation: AEE28124.1 .
AY070089 mRNA. Translation: AAL49783.1 .
AY091341 mRNA. Translation: AAM14280.1 .
AY084794 mRNA. Translation: AAM61361.1 .
PIRi B86209.
RefSeqi NP_172223.1. NM_100617.2.
UniGenei At.28399.

3D structure databases

ProteinModelPortali Q9LNW3.
SMRi Q9LNW3. Positions 119-434.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22496. 12 interactions.
DIPi DIP-40200N.
IntActi Q9LNW3. 3 interactions.

Proteomic databases

PRIDEi Q9LNW3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G07430.1 ; AT1G07430.1 ; AT1G07430 .
GeneIDi 837255.
KEGGi ath:AT1G07430.

Organism-specific databases

TAIRi AT1G07430.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233896.
InParanoidi Q9LNW3.
KOi K14497.
OMAi SANCRCE.
PhylomeDBi Q9LNW3.

Enzyme and pathway databases

BioCyci ARA:AT1G07430-MONOMER.

Gene expression databases

Genevestigatori Q9LNW3.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 2 hits.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Plant PP2C phosphatases: emerging functions in stress signaling."
    Schweighofer A., Hirt H., Meskiene I.
    Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "A protein phosphorylation/dephosphorylation network regulates a plant potassium channel."
    Lee S.-C., Lan W.-Z., Kim B.-G., Li L., Cheong Y.H., Pandey G.K., Lu G., Buchanan B.B., Luan S.
    Proc. Natl. Acad. Sci. U.S.A. 104:15959-15964(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AKT1 AND CIPK23.
  7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C03_ARATH
AccessioniPrimary (citable) accession number: Q9LNW3
Secondary accession number(s): Q8LFK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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